Restriction map for
pF1KSDA0057
(Linear) (Six-base) MAPSORT of: KIAA0057.seq Check: 6513 from: 1 to: 1110
KIAA0057.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1110
Size: 21 1089
AhdI GACnn_n'nnGTC
Cuts at: 0 358 1110
Size: 358 752
AleI CACnn'nnGTG
Cuts at: 0 379 1110
Size: 379 731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 1110
Size: 1063 32 15
Fragments arranged by size:
1063 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 740 878 1110
Size: 740 138 232
Fragments arranged by size:
740 232 138
ApaLI G'TGCA_C
Cuts at: 0 196 460 1110
Size: 196 264 650
Fragments arranged by size:
650 264 196
ApoI r'AATT_y
Cuts at: 0 560 1110
Size: 560 550
AvaI C'yCGr_G
Cuts at: 0 841 1110
Size: 841 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 452 1110
Size: 452 658
BciVI GTATCCnnnnn_n'
Cuts at: 0 409 1110
Size: 409 701
BglI GCCn_nnn'nGGC
Cuts at: 0 638 793 907 1110
Size: 638 155 114 203
Fragments arranged by size:
638 203 155 114
Bme1580I G_kGCm'C
Cuts at: 0 200 464 1110
Size: 200 264 646
Fragments arranged by size:
646 264 200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 634 1110
Size: 634 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 629 1110
Size: 559 70 481
Fragments arranged by size:
559 481 70
Bpu10I CC'TnA_GC
Cuts at: 0 527 628 1110
Size: 527 101 482
Fragments arranged by size:
527 482 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1110
Size: 1084 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 184 1110
Size: 184 926
BsaAI yAC'GTr
Cuts at: 0 399 1110
Size: 399 711
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1063 1093 1110
Size: 1063 30 17
Fragments arranged by size:
1063 30 17
BseYI C'CCAG_C
Cuts at: 0 938 993 1110
Size: 938 55 117
Fragments arranged by size:
938 117 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1110
Size: 499 611
BsiHKAI G_wGCw'C
Cuts at: 0 92 200 464 1110
Size: 92 108 264 646
Fragments arranged by size:
646 264 108 92
Bsp1286I G_dGCh'C
Cuts at: 0 92 200 464 1110
Size: 92 108 264 646
Fragments arranged by size:
646 264 108 92
BsrBI CCG'CTC
Cuts at: 0 33 1110
Size: 33 1077
BstAPI GCAn_nnn'nTGC
Cuts at: 0 878 1110
Size: 878 232
BstEII G'GTnAC_C
Cuts at: 0 759 1039 1110
Size: 759 280 71
Fragments arranged by size:
759 280 71
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1051 1110
Size: 1051 59
Bsu36I CC'TnA_GG
Cuts at: 0 215 1110
Size: 215 895
BtgI C'CryG_G
Cuts at: 0 1084 1110
Size: 1084 26
EaeI y'GGCC_r
Cuts at: 0 794 1110
Size: 794 316
EarI CTCTTCn'nnn_
Cuts at: 0 40 685 703 775 1110
Size: 40 645 18 72 335
Fragments arranged by size:
645 335 72 40 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 559 629 1110
Size: 21 538 70 481
Fragments arranged by size:
538 481 70 21
EcoO109I rG'GnC_Cy
Cuts at: 0 219 1110
Size: 219 891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 153 1110
Size: 153 957
HaeII r_GCGC'y
Cuts at: 0 927 1110
Size: 927 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 344 376 1110
Size: 344 32 734
Fragments arranged by size:
734 344 32
MscI TGG'CCA
Cuts at: 0 796 1110
Size: 796 314
MslI CAynn'nnrTG
Cuts at: 0 62 169 379 1049 1110
Size: 62 107 210 670 61
Fragments arranged by size:
670 210 107 62 61
MspA1I CmG'CkG
Cuts at: 0 357 501 942 1110
Size: 357 144 441 168
Fragments arranged by size:
441 357 168 144
NspI r_CATG'y
Cuts at: 0 268 460 1110
Size: 268 192 650
Fragments arranged by size:
650 268 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 1063 1095 1110
Size: 715 32 316 32 15
Fragments arranged by size:
715 316 32 32 15
PpuMI rG'GwC_Cy
Cuts at: 0 219 1110
Size: 219 891
PstI C_TGCA'G
Cuts at: 0 662 1110
Size: 662 448
PvuII CAG'CTG
Cuts at: 0 357 501 942 1110
Size: 357 144 441 168
Fragments arranged by size:
441 357 168 144
SapI GCTCTTCn'nnn_
Cuts at: 0 40 1110
Size: 40 1070
ScaI AGT'ACT
Cuts at: 0 664 1110
Size: 664 446
SexAI A'CCwGG_T
Cuts at: 0 221 593 1110
Size: 221 372 517
Fragments arranged by size:
517 372 221
SfcI C'TryA_G
Cuts at: 0 176 658 1110
Size: 176 482 452
Fragments arranged by size:
482 452 176
SmlI C'TyrA_G
Cuts at: 0 1099 1110
Size: 1099 11
SphI G_CATG'C
Cuts at: 0 268 1110
Size: 268 842
StuI AGG'CCT
Cuts at: 0 910 1110
Size: 910 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1017 1110
Size: 1017 93
TatI w'GTAC_w
Cuts at: 0 281 662 1110
Size: 281 381 448
Fragments arranged by size:
448 381 281
Tth111I GACn'n_nGTC
Cuts at: 0 223 1110
Size: 223 887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 437 1110
Size: 437 673
XmnI GAAnn'nnTTC
Cuts at: 0 484 730 1110
Size: 484 246 380
Fragments arranged by size:
484 380 246
Enzymes that do cut:
AcuI AhdI AleI AloI AlwNI ApaLI ApoI AvaI
BbsI BciVI BglI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrBI
BstAPI BstEII BstXI Bsu36I BtgI EaeI EarI Eco57MI
EcoO109I FalI HaeII Hin4I MscI MslI MspA1I NspI
PpiI PpuMI PstI PvuII SapI ScaI SexAI SfcI
SmlI SphI StuI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstYI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StyI SwaI TstI
XbaI XhoI XmaI ZraI