Restriction map for
pF1KA0060
(Linear) (Six-base) MAPSORT of: KIAA0060.seq Check: 3508 from: 1 to: 867
KIAA0060.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 373 867
Size: 373 494
AccI GT'mk_AC
Cuts at: 0 335 867
Size: 335 532
AflIII A'CryG_T
Cuts at: 0 665 867
Size: 665 202
AleI CACnn'nnGTG
Cuts at: 0 538 867
Size: 538 329
AvaI C'yCGr_G
Cuts at: 0 233 867
Size: 233 634
BanI G'GyrC_C
Cuts at: 0 540 867
Size: 540 327
BanII G_rGCy'C
Cuts at: 0 116 867
Size: 116 751
BbsI GAAGACnn'nnnn_
Cuts at: 0 205 484 754 867
Size: 205 279 270 113
Fragments arranged by size:
279 270 205 113
BciVI GTATCCnnnnn_n'
Cuts at: 0 486 867
Size: 486 381
Bme1580I G_kGCm'C
Cuts at: 0 545 574 867
Size: 545 29 293
Fragments arranged by size:
545 293 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 867
Size: 131 736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 867
Size: 465 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 435 867
Size: 37 398 432
Fragments arranged by size:
432 398 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 230 867
Size: 230 637
BsaAI yAC'GTr
Cuts at: 0 222 867
Size: 222 645
BsaBI GATnn'nnATC
Cuts at: 0 604 867
Size: 604 263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 867
Size: 664 203
BsiHKAI G_wGCw'C
Cuts at: 0 23 618 867
Size: 23 595 249
Fragments arranged by size:
595 249 23
BsmI GAATG_Cn'
Cuts at: 0 333 867
Size: 333 534
Bsp1286I G_dGCh'C
Cuts at: 0 23 116 545 574 618 867
Size: 23 93 429 29 44 249
Fragments arranged by size:
429 249 93 44 29 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 373 867
Size: 373 494
BsrDI GCAATG_nn'
Cuts at: 0 428 867
Size: 428 439
BsrGI T'GTAC_A
Cuts at: 0 635 806 867
Size: 635 171 61
Fragments arranged by size:
635 171 61
BstBI TT'CG_AA
Cuts at: 0 847 867
Size: 847 20
BtgI C'CryG_G
Cuts at: 0 488 548 867
Size: 488 60 319
Fragments arranged by size:
488 319 60
DraI TTT'AAA
Cuts at: 0 189 867
Size: 189 678
EaeI y'GGCC_r
Cuts at: 0 485 503 867
Size: 485 18 364
Fragments arranged by size:
485 364 18
EarI CTCTTCn'nnn_
Cuts at: 0 359 867
Size: 359 508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 435 867
Size: 37 398 432
Fragments arranged by size:
432 398 37
EcoO109I rG'GnC_Cy
Cuts at: 0 177 467 867
Size: 177 290 400
Fragments arranged by size:
400 290 177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 860 867
Size: 860 7
HincII GTy'rAC
Cuts at: 0 336 867
Size: 336 531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 867
Size: 384 483
MscI TGG'CCA
Cuts at: 0 487 505 867
Size: 487 18 362
Fragments arranged by size:
487 362 18
MslI CAynn'nnrTG
Cuts at: 0 538 577 867
Size: 538 39 290
Fragments arranged by size:
538 290 39
NcoI C'CATG_G
Cuts at: 0 488 548 867
Size: 488 60 319
Fragments arranged by size:
488 319 60
NspI r_CATG'y
Cuts at: 0 669 867
Size: 669 198
PasI CC'CwG_GG
Cuts at: 0 85 108 867
Size: 85 23 759
Fragments arranged by size:
759 85 23
PciI A'CATG_T
Cuts at: 0 665 867
Size: 665 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 867
Size: 318 549
PfoI T'CCnGG_A
Cuts at: 0 14 463 867
Size: 14 449 404
Fragments arranged by size:
449 404 14
PpuMI rG'GwC_Cy
Cuts at: 0 177 467 867
Size: 177 290 400
Fragments arranged by size:
400 290 177
PstI C_TGCA'G
Cuts at: 0 384 867
Size: 384 483
SalI G'TCGA_C
Cuts at: 0 334 867
Size: 334 533
ScaI AGT'ACT
Cuts at: 0 100 867
Size: 100 767
SfcI C'TryA_G
Cuts at: 0 340 380 867
Size: 340 40 487
Fragments arranged by size:
487 340 40
SmlI C'TyrA_G
Cuts at: 0 233 867
Size: 233 634
StyI C'CwwG_G
Cuts at: 0 488 536 548 731 867
Size: 488 48 12 183 136
Fragments arranged by size:
488 183 136 48 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 314 325 867
Size: 314 11 542
Fragments arranged by size:
542 314 11
TatI w'GTAC_w
Cuts at: 0 98 635 806 867
Size: 98 537 171 61
Fragments arranged by size:
537 171 98 61
Tth111I GACn'n_nGTC
Cuts at: 0 675 867
Size: 675 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 319 867
Size: 319 548
XhoI C'TCGA_G
Cuts at: 0 233 867
Size: 233 634
XmnI GAAnn'nnTTC
Cuts at: 0 100 202 274 867
Size: 100 102 72 593
Fragments arranged by size:
593 102 100 72
Enzymes that do cut:
AarI AccI AflIII AleI AvaI BanI BanII BbsI
BciVI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaBI
BseRI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstBI
BtgI DraI EaeI EarI Eco57MI EcoO109I FalI HincII
MmeI MscI MslI NcoI NspI PasI PciI PflMI
PfoI PpuMI PstI SalI ScaI SfcI SmlI StyI
TaqII TatI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XmaI ZraI