Restriction map for
pF1KSDA0062
(Linear) (Six-base) MAPSORT of: KIAA0062.seq Check: 6574 from: 1 to: 1476
KIAA0062.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1476
Size: 205 1271
AfeI AGC'GCT
Cuts at: 0 117 447 1476
Size: 117 330 1029
Fragments arranged by size:
1029 330 117
AflII C'TTAA_G
Cuts at: 0 733 1476
Size: 733 743
AleI CACnn'nnGTG
Cuts at: 0 1108 1476
Size: 1108 368
AlwNI CAG_nnn'CTG
Cuts at: 0 183 372 942 1407 1476
Size: 183 189 570 465 69
Fragments arranged by size:
570 465 189 183 69
ApaLI G'TGCA_C
Cuts at: 0 101 1476
Size: 101 1375
AvaI C'yCGr_G
Cuts at: 0 380 1476
Size: 380 1096
BanI G'GyrC_C
Cuts at: 0 522 891 1476
Size: 522 369 585
Fragments arranged by size:
585 522 369
BanII G_rGCy'C
Cuts at: 0 341 925 1476
Size: 341 584 551
Fragments arranged by size:
584 551 341
BbeI G_GCGC'C
Cuts at: 0 895 1476
Size: 895 581
BbsI GAAGACnn'nnnn_
Cuts at: 0 544 1476
Size: 544 932
BbvCI CC'TCA_GC
Cuts at: 0 860 1476
Size: 860 616
BciVI GTATCCnnnnn_n'
Cuts at: 0 457 1476
Size: 457 1019
BclI T'GATC_A
Cuts at: 0 1016 1476
Size: 1016 460
BglII A'GATC_T
Cuts at: 0 716 1476
Size: 716 760
Bme1580I G_kGCm'C
Cuts at: 0 105 527 1476
Size: 105 422 949
Fragments arranged by size:
949 422 105
BmtI G_CTAG'C
Cuts at: 0 1294 1476
Size: 1294 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 325 349 1316 1476
Size: 302 23 24 967 160
Fragments arranged by size:
967 302 160 24 23
Bpu10I CC'TnA_GC
Cuts at: 0 860 1476
Size: 860 616
BsaI GGTCTCn'nnnn_
Cuts at: 0 278 475 1476
Size: 278 197 1001
Fragments arranged by size:
1001 278 197
BsaAI yAC'GTr
Cuts at: 0 243 269 1476
Size: 243 26 1207
Fragments arranged by size:
1207 243 26
BsaBI GATnn'nnATC
Cuts at: 0 1180 1476
Size: 1180 296
BsaHI Gr'CG_yC
Cuts at: 0 892 1476
Size: 892 584
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1139 1169 1476
Size: 1139 30 307
Fragments arranged by size:
1139 307 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 445 463 823 1141 1476
Size: 427 18 18 360 318 335
Fragments arranged by size:
427 360 335 318 18 18
BseYI C'CCAG_C
Cuts at: 0 236 1171 1476
Size: 236 935 305
Fragments arranged by size:
935 305 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 372 955 1476
Size: 1 371 583 521
Fragments arranged by size:
583 521 371 1
BsiHKAI G_wGCw'C
Cuts at: 0 105 341 1476
Size: 105 236 1135
Fragments arranged by size:
1135 236 105
Bsp1286I G_dGCh'C
Cuts at: 0 105 341 527 925 1476
Size: 105 236 186 398 551
Fragments arranged by size:
551 398 236 186 105
BspHI T'CATG_A
Cuts at: 0 530 1476
Size: 530 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 950 1476
Size: 950 526
BsrFI r'CCGG_y
Cuts at: 0 1255 1476
Size: 1255 221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 942 1476
Size: 942 534
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1437 1476
Size: 1437 39
BstYI r'GATC_y
Cuts at: 0 135 716 1463 1476
Size: 135 581 747 13
Fragments arranged by size:
747 581 135 13
Bsu36I CC'TnA_GG
Cuts at: 0 77 1476
Size: 77 1399
BtgI C'CryG_G
Cuts at: 0 896 1476
Size: 896 580
BtsI GCAGTG_nn'
Cuts at: 0 293 680 864 879 1476
Size: 293 387 184 15 597
Fragments arranged by size:
597 387 293 184 15
ClaI AT'CG_AT
Cuts at: 0 1049 1476
Size: 1049 427
DrdI GACnn_nn'nnGTC
Cuts at: 0 910 1476
Size: 910 566
EaeI y'GGCC_r
Cuts at: 0 439 1058 1112 1253 1476
Size: 439 619 54 141 223
Fragments arranged by size:
619 439 223 141 54
EarI CTCTTCn'nnn_
Cuts at: 0 295 613 1201 1476
Size: 295 318 588 275
Fragments arranged by size:
588 318 295 275
EcoICRI GAG'CTC
Cuts at: 0 339 1476
Size: 339 1137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 302 325 349 1316 1476
Size: 205 97 23 24 967 160
Fragments arranged by size:
967 205 160 97 24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1123 1476
Size: 1123 353
EcoO109I rG'GnC_Cy
Cuts at: 0 189 840 939 1476
Size: 189 651 99 537
Fragments arranged by size:
651 537 189 99
EcoRV GAT'ATC
Cuts at: 0 996 1476
Size: 996 480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 558 1476
Size: 558 918
HaeII r_GCGC'y
Cuts at: 0 119 449 895 1476
Size: 119 330 446 581
Fragments arranged by size:
581 446 330 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 1005 1037 1139 1171 1415 1447
Size: 455 32 518 32 102 32 244 32
Cuts at: 1447 1476
Size: 29
Fragments arranged by size:
518 455 244 102 32 32 32 32
29
KasI G'GCGC_C
Cuts at: 0 891 1476
Size: 891 585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1209 1476
Size: 623 586 267
Fragments arranged by size:
623 586 267
MscI TGG'CCA
Cuts at: 0 1060 1114 1476
Size: 1060 54 362
Fragments arranged by size:
1060 362 54
MslI CAynn'nnrTG
Cuts at: 0 149 206 755 1108 1432 1435 1476
Size: 149 57 549 353 324 3 41
Fragments arranged by size:
549 353 324 149 57 41 3
MspA1I CmG'CkG
Cuts at: 0 183 372 1476
Size: 183 189 1104
Fragments arranged by size:
1104 189 183
NaeI GCC'GGC
Cuts at: 0 1257 1476
Size: 1257 219
NarI GG'CG_CC
Cuts at: 0 892 1476
Size: 892 584
NcoI C'CATG_G
Cuts at: 0 896 1476
Size: 896 580
NgoMIV G'CCGG_C
Cuts at: 0 1255 1476
Size: 1255 221
NheI G'CTAG_C
Cuts at: 0 1290 1476
Size: 1290 186
PasI CC'CwG_GG
Cuts at: 0 96 1476
Size: 96 1380
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 1407 1476
Size: 372 1035 69
Fragments arranged by size:
1035 372 69
PfoI T'CCnGG_A
Cuts at: 0 341 620 1476
Size: 341 279 856
Fragments arranged by size:
856 341 279
PmlI CAC'GTG
Cuts at: 0 243 269 1476
Size: 243 26 1207
Fragments arranged by size:
1207 243 26
PpuMI rG'GwC_Cy
Cuts at: 0 840 939 1476
Size: 840 99 537
Fragments arranged by size:
840 537 99
PstI C_TGCA'G
Cuts at: 0 134 179 675 833 871 947 1476
Size: 134 45 496 158 38 76 529
Fragments arranged by size:
529 496 158 134 76 45 38
PvuII CAG'CTG
Cuts at: 0 183 372 1476
Size: 183 189 1104
Fragments arranged by size:
1104 189 183
SacI G_AGCT'C
Cuts at: 0 341 1476
Size: 341 1135
SbfI CC_TGCA'GG
Cuts at: 0 134 947 1476
Size: 134 813 529
Fragments arranged by size:
813 529 134
SfcI C'TryA_G
Cuts at: 0 130 175 671 829 867 943 1476
Size: 130 45 496 158 38 76 533
Fragments arranged by size:
533 496 158 130 76 45 38
SfoI GGC'GCC
Cuts at: 0 893 1476
Size: 893 583
SmaI CCC'GGG
Cuts at: 0 382 1476
Size: 382 1094
SmlI C'TyrA_G
Cuts at: 0 733 1476
Size: 733 743
StyI C'CwwG_G
Cuts at: 0 896 1092 1476
Size: 896 196 384
Fragments arranged by size:
896 384 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 250 1476
Size: 88 162 1226
Fragments arranged by size:
1226 162 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 1476
Size: 76 32 1368
Fragments arranged by size:
1368 76 32
Tth111I GACn'n_nGTC
Cuts at: 0 1153 1476
Size: 1153 323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 1476
Size: 672 804
XmaI C'CCGG_G
Cuts at: 0 380 1476
Size: 380 1096
Enzymes that do cut:
AcuI AfeI AflII AleI AlwNI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII Bme1580I
BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI PasI PflMI PfoI PmlI
PpuMI PstI PvuII SacI SbfI SfcI SfoI SmaI
SmlI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BfrBI BglI BlpI BmgBI BmrI
BplI BpuEI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI CspCI DraI DraIII EagI EciI EcoRI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI XbaI XhoI XmnI ZraI