Restriction map for pF1KSDA0063
 (Linear) (Six-base) MAPSORT of: KIAA0063.seq  Check: 8046  from: 1  to: 606



KIAA0063.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

Acc65I G'GTAC_C

Cuts at:      0     561     606 
   Size:        561      45
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     606 
   Size:        256     350
 
AleI CACnn'nnGTG

Cuts at:      0     202     606 
   Size:        202     404
 
AvaI C'yCGr_G

Cuts at:      0     155     478     606 
   Size:        155     323     128

  Fragments arranged by size: 

                 323     155     128
 
BanI G'GyrC_C

Cuts at:      0     561     606 
   Size:        561      45
 
BanII G_rGCy'C

Cuts at:      0     506     606 
   Size:        506     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     123     606 
   Size:        123     483
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     300     334     606 
   Size:        300      34     272

  Fragments arranged by size: 

                 300     272      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     153     606 
   Size:        153     453
 
Bme1580I G_kGCm'C

Cuts at:      0     111     606 
   Size:        111     495
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     206     606 
   Size:        206     400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     606 
   Size:        354     252
 
Bpu10I CC'TnA_GC

Cuts at:      0     505     606 
   Size:        505     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     606 
   Size:        454     152
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     606 
   Size:        445      30     131

  Fragments arranged by size: 

                 445     131      30
 
BseYI C'CCAG_C

Cuts at:      0     228     380     606 
   Size:        228     152     226

  Fragments arranged by size: 

                 228     226     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     506     606 
   Size:        506     100
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     506     606 
   Size:        111     395     100

  Fragments arranged by size: 

                 395     111     100
 
BspHI T'CATG_A

Cuts at:      0     356     606 
   Size:        356     250
 
BsrDI GCAATG_nn'

Cuts at:      0     150     326     606 
   Size:        150     176     280

  Fragments arranged by size: 

                 280     176     150
 
BstYI r'GATC_y

Cuts at:      0     419     606 
   Size:        419     187
 
BtgI C'CryG_G

Cuts at:      0      13     200     606 
   Size:         13     187     406

  Fragments arranged by size: 

                 406     187      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     214     563     606 
   Size:        214     349      43

  Fragments arranged by size: 

                 349     214      43
 
EcoICRI GAG'CTC

Cuts at:      0     504     606 
   Size:        504     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     354     606 
   Size:        256      98     252

  Fragments arranged by size: 

                 256     252      98
 
EcoO109I rG'GnC_Cy

Cuts at:      0     443     606 
   Size:        443     163
 
KpnI G_GTAC'C

Cuts at:      0     565     606 
   Size:        565      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     606 
   Size:        571      35
 
MslI CAynn'nnrTG

Cuts at:      0     202     606 
   Size:        202     404
 
NcoI C'CATG_G

Cuts at:      0      13     200     606 
   Size:         13     187     406

  Fragments arranged by size: 

                 406     187      13
 
NspI r_CATG'y

Cuts at:      0     228     606 
   Size:        228     378
 
PfoI T'CCnGG_A

Cuts at:      0     134     606 
   Size:        134     472
 
SacI G_AGCT'C

Cuts at:      0     506     606 
   Size:        506     100
 
SapI GCTCTTCn'nnn_

Cuts at:      0     214     606 
   Size:        214     392
 
SmaI CCC'GGG

Cuts at:      0     157     606 
   Size:        157     449
 
SmlI C'TyrA_G

Cuts at:      0     469     606 
   Size:        469     137
 
SphI G_CATG'C

Cuts at:      0     228     606 
   Size:        228     378
 
StyI C'CwwG_G

Cuts at:      0      13     200     606 
   Size:         13     187     406

  Fragments arranged by size: 

                 406     187      13
 
XmaI C'CCGG_G

Cuts at:      0     155     606 
   Size:        155     451
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AvaI     BanI    BanII     BbsI     BcgI
    BciVI Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BsaXI    BseYI
  BsiHKAI Bsp1286I    BspHI    BsrDI    BstYI     BtgI     EarI  EcoICRI
  Eco57MI EcoO109I     KpnI     MmeI     MslI     NcoI     NspI     PfoI
     SacI     SapI     SmaI     SmlI     SphI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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