Restriction map for
pF1KSDA0067
(Linear) (Six-base) MAPSORT of: KIAA0067.seq Check: 4563 from: 1 to: 3873
KIAA0067.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1377 1395 1402 3873
Size: 1377 18 7 2471
Fragments arranged by size:
2471 1377 18 7
AatII G_ACGT'C
Cuts at: 0 3867 3873
Size: 3867 6
AccI GT'mk_AC
Cuts at: 0 720 2319 2648 3873
Size: 720 1599 329 1225
Fragments arranged by size:
1599 1225 720 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 2714 2730 3230 3440 3873
Size: 77 2637 16 500 210 433
Fragments arranged by size:
2637 500 433 210 77 16
AflII C'TTAA_G
Cuts at: 0 468 3873
Size: 468 3405
AflIII A'CryG_T
Cuts at: 0 1252 2369 3873
Size: 1252 1117 1504
Fragments arranged by size:
1504 1252 1117
AgeI A'CCGG_T
Cuts at: 0 1315 2381 3873
Size: 1315 1066 1492
Fragments arranged by size:
1492 1315 1066
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 2390 2422 3873
Size: 1177 32 1181 32 1451
Fragments arranged by size:
1451 1181 1177 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 537 1219 1311 1492 1603 2167 3873
Size: 537 682 92 181 111 564 1706
Fragments arranged by size:
1706 682 564 537 181 111 92
ApaI G_GGCC'C
Cuts at: 0 1674 3873
Size: 1674 2199
ApaLI G'TGCA_C
Cuts at: 0 2373 3873
Size: 2373 1500
ApoI r'AATT_y
Cuts at: 0 608 697 2170 3873
Size: 608 89 1473 1703
Fragments arranged by size:
1703 1473 608 89
AvaI C'yCGr_G
Cuts at: 0 1094 1751 2136 2792 3491 3873
Size: 1094 657 385 656 699 382
Fragments arranged by size:
1094 699 657 656 385 382
BamHI G'GATC_C
Cuts at: 0 1127 1980 3873
Size: 1127 853 1893
Fragments arranged by size:
1893 1127 853
BanI G'GyrC_C
Cuts at: 0 646 3873
Size: 646 3227
BanII G_rGCy'C
Cuts at: 0 1190 1501 1674 1685 3873
Size: 1190 311 173 11 2188
Fragments arranged by size:
2188 1190 311 173 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 980 1641 3264 3696 3859 3873
Size: 980 661 1623 432 163 14
Fragments arranged by size:
1623 980 661 432 163 14
BbvCI CC'TCA_GC
Cuts at: 0 1212 1617 3873
Size: 1212 405 2256
Fragments arranged by size:
2256 1212 405
BciVI GTATCCnnnnn_n'
Cuts at: 0 941 2143 2458 3705 3873
Size: 941 1202 315 1247 168
Fragments arranged by size:
1247 1202 941 315 168
BfrBI ATG'CAT
Cuts at: 0 253 3246 3873
Size: 253 2993 627
Fragments arranged by size:
2993 627 253
BglII A'GATC_T
Cuts at: 0 2850 3873
Size: 2850 1023
Bme1580I G_kGCm'C
Cuts at: 0 1674 2377 3873
Size: 1674 703 1496
Fragments arranged by size:
1674 1496 703
BmgBI CAC'GTC
Cuts at: 0 1255 1475 3873
Size: 1255 220 2398
Fragments arranged by size:
2398 1255 220
BmrI ACTGGGnnnn_n'
Cuts at: 0 106 1323 1437 1639 3873
Size: 106 1217 114 202 2234
Fragments arranged by size:
2234 1217 202 114 106
BmtI G_CTAG'C
Cuts at: 0 184 3873
Size: 184 3689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 446 3087 3873
Size: 446 2641 786
Fragments arranged by size:
2641 786 446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 587 1204 1246 1619 1684 1984 3873
Size: 163 424 617 42 373 65 300 1889
Fragments arranged by size:
1889 617 424 373 300 163 65 42
Bpu10I CC'TnA_GC
Cuts at: 0 871 1212 1246 1617 3873
Size: 871 341 34 371 2256
Fragments arranged by size:
2256 871 371 341 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 573 1027 3873
Size: 467 106 454 2846
Fragments arranged by size:
2846 467 454 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 1900 3873
Size: 1900 1973
BsaAI yAC'GTr
Cuts at: 0 1082 3873
Size: 1082 2791
BsaBI GATnn'nnATC
Cuts at: 0 812 3873
Size: 812 3061
BsaHI Gr'CG_yC
Cuts at: 0 1842 3864 3873
Size: 1842 2022 9
Fragments arranged by size:
2022 1842 9
BsaWI w'CCGG_w
Cuts at: 0 1315 2381 3873
Size: 1315 1066 1492
Fragments arranged by size:
1492 1315 1066
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 283 313 1177 1207 2085 2115 3873
Size: 283 30 864 30 878 30 1758
Fragments arranged by size:
1758 878 864 283 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1015 1117 1290 3625 3873
Size: 1015 102 173 2335 248
Fragments arranged by size:
2335 1015 248 173 102
BseYI C'CCAG_C
Cuts at: 0 306 418 1608 1674 2441 3040 3769 3873
Size: 306 112 1190 66 767 599 729 104
Fragments arranged by size:
1190 767 729 599 306 112 104 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 1511 3873
Size: 1205 306 2362
Fragments arranged by size:
2362 1205 306
BsiEI CG_ry'CG
Cuts at: 0 3141 3873
Size: 3141 732
BsiHKAI G_wGCw'C
Cuts at: 0 1474 1698 2377 3873
Size: 1474 224 679 1496
Fragments arranged by size:
1496 1474 679 224
BsmI GAATG_Cn'
Cuts at: 0 3859 3873
Size: 3859 14
Bsp1286I G_dGCh'C
Cuts at: 0 1190 1474 1501 1674 1685 1698 2377 3873
Size: 1190 284 27 173 11 13 679 1496
Fragments arranged by size:
1496 1190 679 284 173 27 13 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1377 1395 1402 1446 3873
Size: 1377 18 7 44 2427
Fragments arranged by size:
2427 1377 44 18 7
BsrBI CCG'CTC
Cuts at: 0 1668 3873
Size: 1668 2205
BsrDI GCAATG_nn'
Cuts at: 0 2465 3873
Size: 2465 1408
BsrFI r'CCGG_y
Cuts at: 0 941 1315 2381 3873
Size: 941 374 1066 1492
Fragments arranged by size:
1492 1066 941 374
BsrGI T'GTAC_A
Cuts at: 0 159 3873
Size: 159 3714
BstEII G'GTnAC_C
Cuts at: 0 586 3873
Size: 586 3287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 670 2668 3873
Size: 670 1998 1205
Fragments arranged by size:
1998 1205 670
BstYI r'GATC_y
Cuts at: 0 534 1127 1160 1308 1489 1980 2850 3873
Size: 534 593 33 148 181 491 870 1023
Fragments arranged by size:
1023 870 593 534 491 181 148 33
Bsu36I CC'TnA_GG
Cuts at: 0 3078 3873
Size: 3078 795
BtgI C'CryG_G
Cuts at: 0 1031 1653 1700 3516 3873
Size: 1031 622 47 1816 357
Fragments arranged by size:
1816 1031 622 357 47
BtsI GCAGTG_nn'
Cuts at: 0 89 269 1010 2646 3353 3816 3873
Size: 89 180 741 1636 707 463 57
Fragments arranged by size:
1636 741 707 463 180 89 57
ClaI AT'CG_AT
Cuts at: 0 131 3873
Size: 131 3742
DraIII CAC_nnn'GTG
Cuts at: 0 1085 1534 1891 3873
Size: 1085 449 357 1982
Fragments arranged by size:
1982 1085 449 357
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 3873
Size: 595 3278
EaeI y'GGCC_r
Cuts at: 0 943 1050 3873
Size: 943 107 2823
Fragments arranged by size:
2823 943 107
EarI CTCTTCn'nnn_
Cuts at: 0 53 514 616 1138 1963 2306 2690 2915
Size: 53 461 102 522 825 343 384 225
Cuts at: 2915 3873
Size: 958
Fragments arranged by size:
958 825 522 461 384 343 225 102
53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1842 2781 3873
Size: 1842 939 1092
Fragments arranged by size:
1842 1092 939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 163 587 1204 1246 1619 1684 1984
Size: 77 86 424 617 42 373 65 300
Cuts at: 1984 2714 2730 3230 3440 3873
Size: 730 16 500 210 433
Fragments arranged by size:
730 617 500 433 424 373 300 210
86 77 65 42 16
EcoO109I rG'GnC_Cy
Cuts at: 0 1285 1671 2164 2688 3873
Size: 1285 386 493 524 1185
Fragments arranged by size:
1285 1185 524 493 386
EcoRI G'AATT_C
Cuts at: 0 608 3873
Size: 608 3265
EcoRV GAT'ATC
Cuts at: 0 3396 3873
Size: 3396 477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 667 1881 3873
Size: 667 1214 1992
Fragments arranged by size:
1992 1214 667
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 283 315 316 328 348 360
Size: 238 32 13 32 1 12 20 12
Cuts at: 360 2258 2290 2698 2730 3074 3106 3873
Size: 1898 32 408 32 344 32 767
Fragments arranged by size:
1898 767 408 344 238 32 32 32
32 32 20 13 12 12 1
HincII GTy'rAC
Cuts at: 0 234 513 1851 3384 3873
Size: 234 279 1338 1533 489
Fragments arranged by size:
1533 1338 489 279 234
HindIII A'AGCT_T
Cuts at: 0 1708 3637 3873
Size: 1708 1929 236
Fragments arranged by size:
1929 1708 236
HpaI GTT'AAC
Cuts at: 0 1851 3873
Size: 1851 2022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 3305 3873
Size: 902 2403 568
Fragments arranged by size:
2403 902 568
MscI TGG'CCA
Cuts at: 0 1052 3873
Size: 1052 2821
MspA1I CmG'CkG
Cuts at: 0 2453 2678 3379 3873
Size: 2453 225 701 494
Fragments arranged by size:
2453 701 494 225
NaeI GCC'GGC
Cuts at: 0 943 3873
Size: 943 2930
NcoI C'CATG_G
Cuts at: 0 1031 1653 1700 3516 3873
Size: 1031 622 47 1816 357
Fragments arranged by size:
1816 1031 622 357 47
NdeI CA'TA_TG
Cuts at: 0 1986 3873
Size: 1986 1887
NgoMIV G'CCGG_C
Cuts at: 0 941 3873
Size: 941 2932
NheI G'CTAG_C
Cuts at: 0 180 3873
Size: 180 3693
NsiI A_TGCA'T
Cuts at: 0 255 3248 3873
Size: 255 2993 625
Fragments arranged by size:
2993 625 255
NspI r_CATG'y
Cuts at: 0 606 1662 2373 3873
Size: 606 1056 711 1500
Fragments arranged by size:
1500 1056 711 606
PasI CC'CwG_GG
Cuts at: 0 3734 3873
Size: 3734 139
PciI A'CATG_T
Cuts at: 0 2369 3873
Size: 2369 1504
PflMI CCAn_nnn'nTGG
Cuts at: 0 2854 3873
Size: 2854 1019
PfoI T'CCnGG_A
Cuts at: 0 1136 1961 3873
Size: 1136 825 1912
Fragments arranged by size:
1912 1136 825
PmlI CAC'GTG
Cuts at: 0 1082 3873
Size: 1082 2791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 2800 2832 3873
Size: 1177 32 1591 32 1041
Fragments arranged by size:
1591 1177 1041 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2688 3873
Size: 2688 1185
PspOMI G'GGCC_C
Cuts at: 0 1670 3873
Size: 1670 2203
PstI C_TGCA'G
Cuts at: 0 1735 3873
Size: 1735 2138
SapI GCTCTTCn'nnn_
Cuts at: 0 514 3873
Size: 514 3359
ScaI AGT'ACT
Cuts at: 0 2557 3873
Size: 2557 1316
SfcI C'TryA_G
Cuts at: 0 44 356 1358 1731 2118 2249 2413 2891
Size: 44 312 1002 373 387 131 164 478
Cuts at: 2891 3374 3873
Size: 483 499
Fragments arranged by size:
1002 499 483 478 387 373 312 164
131 44
SmaI CCC'GGG
Cuts at: 0 2138 2794 3873
Size: 2138 656 1079
Fragments arranged by size:
2138 1079 656
SmlI C'TyrA_G
Cuts at: 0 468 482 552 1042 1751 3873
Size: 468 14 70 490 709 2122
Fragments arranged by size:
2122 709 490 468 70 14
SpeI A'CTAG_T
Cuts at: 0 3238 3873
Size: 3238 635
SphI G_CATG'C
Cuts at: 0 606 1662 3873
Size: 606 1056 2211
Fragments arranged by size:
2211 1056 606
StuI AGG'CCT
Cuts at: 0 3070 3873
Size: 3070 803
StyI C'CwwG_G
Cuts at: 0 1031 1653 1700 2831 3516 3873
Size: 1031 622 47 1131 685 357
Fragments arranged by size:
1131 1031 685 622 357 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 5 1255 2015 2189 2536 2943 3155 3726
Size: 5 1250 760 174 347 407 212 571
Cuts at: 3726 3873
Size: 147
Fragments arranged by size:
1250 760 571 407 347 212 174 147
5
TatI w'GTAC_w
Cuts at: 0 159 1298 2297 2555 3873
Size: 159 1139 999 258 1318
Fragments arranged by size:
1318 1139 999 258 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1066 1098 2085 2117 3873
Size: 1066 32 987 32 1756
Fragments arranged by size:
1756 1066 987 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3303 3873
Size: 3303 570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1029 2937 3601 3873
Size: 1029 1908 664 272
Fragments arranged by size:
1908 1029 664 272
XhoI C'TCGA_G
Cuts at: 0 1751 3873
Size: 1751 2122
XmaI C'CCGG_G
Cuts at: 0 2136 2792 3873
Size: 2136 656 1081
Fragments arranged by size:
2136 1081 656
XmnI GAAnn'nnTTC
Cuts at: 0 292 1711 2063 3215 3703 3873
Size: 292 1419 352 1152 488 170
Fragments arranged by size:
1419 1152 488 352 292 170
ZraI GAC'GTC
Cuts at: 0 3865 3873
Size: 3865 8
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BfrBI BglII Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI
EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII HpaI MmeI MscI MspA1I NaeI
NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI SapI
ScaI SfcI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AhdI AleI AlfI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BclI BglI BlpI
BsiWI BsmBI BspEI BspHI BssHII BssSI BstAPI BstBI
BstZ17I BtgZI CspCI DraI EagI EcoICRI EcoNI FseI
FspI FspAI HaeII KasI KpnI MfeI MluI MslI
NarI NotI NruI PacI PmeI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI
SspI SwaI XbaI