Restriction map for
pF1KA0068
(Linear) (Six-base) MAPSORT of: KIAA0068.seq Check: 8940 from: 1 to: 3759
KIAA0068.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1374 3759
Size: 1374 2385
AccI GT'mk_AC
Cuts at: 0 3410 3759
Size: 3410 349
Acc65I G'GTAC_C
Cuts at: 0 2441 3759
Size: 2441 1318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1316 2269 2821 2959 2986 3697 3759
Size: 541 775 953 552 138 27 711 62
Fragments arranged by size:
953 775 711 552 541 138 62 27
AfeI AGC'GCT
Cuts at: 0 1293 3759
Size: 1293 2466
AflIII A'CryG_T
Cuts at: 0 497 2492 3759
Size: 497 1995 1267
Fragments arranged by size:
1995 1267 497
AgeI A'CCGG_T
Cuts at: 0 2585 3759
Size: 2585 1174
AhdI GACnn_n'nnGTC
Cuts at: 0 30 456 3759
Size: 30 426 3303
Fragments arranged by size:
3303 426 30
AleI CACnn'nnGTG
Cuts at: 0 3703 3759
Size: 3703 56
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2660 2851 3759
Size: 2660 191 908
Fragments arranged by size:
2660 908 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1396 1428 3759
Size: 1396 32 2331
Fragments arranged by size:
2331 1396 32
AlwNI CAG_nnn'CTG
Cuts at: 0 627 669 1191 1389 2058 2661 2988 3039
Size: 627 42 522 198 669 603 327 51
Cuts at: 3039 3759
Size: 720
Fragments arranged by size:
720 669 627 603 522 327 198 51
42
ApaI G_GGCC'C
Cuts at: 0 1760 3759
Size: 1760 1999
ApaLI G'TGCA_C
Cuts at: 0 999 1249 3759
Size: 999 250 2510
Fragments arranged by size:
2510 999 250
ApoI r'AATT_y
Cuts at: 0 120 369 487 1780 1818 2620 3620 3759
Size: 120 249 118 1293 38 802 1000 139
Fragments arranged by size:
1293 1000 802 249 139 120 118 38
AvaI C'yCGr_G
Cuts at: 0 269 1122 3759
Size: 269 853 2637
Fragments arranged by size:
2637 853 269
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2757 2790 3759
Size: 2757 33 969
Fragments arranged by size:
2757 969 33
BamHI G'GATC_C
Cuts at: 0 1904 1937 2668 3296 3759
Size: 1904 33 731 628 463
Fragments arranged by size:
1904 731 628 463 33
BanI G'GyrC_C
Cuts at: 0 422 2441 3759
Size: 422 2019 1318
Fragments arranged by size:
2019 1318 422
BanII G_rGCy'C
Cuts at: 0 92 710 1123 1580 1760 2540 3759
Size: 92 618 413 457 180 780 1219
Fragments arranged by size:
1219 780 618 457 413 180 92
BbeI G_GCGC'C
Cuts at: 0 426 3759
Size: 426 3333
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 1150 1552 2520 2854 2974 3759
Size: 146 1004 402 968 334 120 785
Fragments arranged by size:
1004 968 785 402 334 146 120
BbvCI CC'TCA_GC
Cuts at: 0 3206 3759
Size: 3206 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 980 1014 1152 1186 2171 2205 3759
Size: 980 34 138 34 985 34 1554
Fragments arranged by size:
1554 985 980 138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2923 3759
Size: 2923 836
BclI T'GATC_A
Cuts at: 0 473 1370 2300 3488 3759
Size: 473 897 930 1188 271
Fragments arranged by size:
1188 930 897 473 271
BfrBI ATG'CAT
Cuts at: 0 151 3357 3759
Size: 151 3206 402
Fragments arranged by size:
3206 402 151
BglI GCCn_nnn'nGGC
Cuts at: 0 1488 3759
Size: 1488 2271
BglII A'GATC_T
Cuts at: 0 2873 3759
Size: 2873 886
BlpI GC'TnA_GC
Cuts at: 0 2434 3759
Size: 2434 1325
Bme1580I G_kGCm'C
Cuts at: 0 1003 1253 1760 3700 3759
Size: 1003 250 507 1940 59
Fragments arranged by size:
1940 1003 507 250 59
BmgBI CAC'GTC
Cuts at: 0 998 2526 3759
Size: 998 1528 1233
Fragments arranged by size:
1528 1233 998
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 764 1569 3205 3483 3759
Size: 490 274 805 1636 278 276
Fragments arranged by size:
1636 805 490 278 276 274
BmtI G_CTAG'C
Cuts at: 0 2119 3759
Size: 2119 1640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 590 2919 3759
Size: 590 2329 840
Fragments arranged by size:
2329 840 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 70 238 276 364 889 1647 1903
Size: 40 30 168 38 88 525 758 256
Cuts at: 1903 1978 2679 2938 3759
Size: 75 701 259 821
Fragments arranged by size:
821 758 701 525 259 256 168 88
75 40 38 30
Bpu10I CC'TnA_GC
Cuts at: 0 3206 3759
Size: 3206 553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1758 1771 3759
Size: 1758 13 1988
Fragments arranged by size:
1988 1758 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 1954 3759
Size: 1954 1805
BsaAI yAC'GTr
Cuts at: 0 586 1221 1989 2844 3759
Size: 586 635 768 855 915
Fragments arranged by size:
915 855 768 635 586
BsaBI GATnn'nnATC
Cuts at: 0 1051 3759
Size: 1051 2708
BsaHI Gr'CG_yC
Cuts at: 0 423 766 2465 3759
Size: 423 343 1699 1294
Fragments arranged by size:
1699 1294 423 343
BsaWI w'CCGG_w
Cuts at: 0 2585 2714 3299 3759
Size: 2585 129 585 460
Fragments arranged by size:
2585 585 460 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 819 849 1252 1282 1396 1426 3759
Size: 819 30 403 30 114 30 2333
Fragments arranged by size:
2333 819 403 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 235 273 456 1351 2260 2806 3759
Size: 67 168 38 183 895 909 546 953
Fragments arranged by size:
953 909 895 546 183 168 67 38
BseYI C'CCAG_C
Cuts at: 0 1590 2306 3340 3759
Size: 1590 716 1034 419
Fragments arranged by size:
1590 1034 716 419
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 2284 2506 3052 3759
Size: 555 1729 222 546 707
Fragments arranged by size:
1729 707 555 546 222
BsiHKAI G_wGCw'C
Cuts at: 0 710 1003 1253 1994 2540 3759
Size: 710 293 250 741 546 1219
Fragments arranged by size:
1219 741 710 546 293 250
BsmI GAATG_Cn'
Cuts at: 0 2194 3416 3759
Size: 2194 1222 343
Fragments arranged by size:
2194 1222 343
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1558 2233 2319 3759
Size: 1558 675 86 1440
Fragments arranged by size:
1558 1440 675 86
Bsp1286I G_dGCh'C
Cuts at: 0 92 710 1003 1123 1253 1580 1760 1994
Size: 92 618 293 120 130 327 180 234
Cuts at: 1994 2540 3700 3759
Size: 546 1160 59
Fragments arranged by size:
1160 618 546 327 293 234 180 130
120 92 59
BspEI T'CCGG_A
Cuts at: 0 3299 3759
Size: 3299 460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1374 2996 3071 3245 3759
Size: 1374 1622 75 174 514
Fragments arranged by size:
1622 1374 514 174 75
BsrBI CCG'CTC
Cuts at: 0 574 1622 3759
Size: 574 1048 2137
Fragments arranged by size:
2137 1048 574
BsrDI GCAATG_nn'
Cuts at: 0 1706 3351 3759
Size: 1706 1645 408
Fragments arranged by size:
1706 1645 408
BsrFI r'CCGG_y
Cuts at: 0 2438 2585 2753 3759
Size: 2438 147 168 1006
Fragments arranged by size:
2438 1006 168 147
BsrGI T'GTAC_A
Cuts at: 0 248 2009 3759
Size: 248 1761 1750
Fragments arranged by size:
1761 1750 248
BssHII G'CGCG_C
Cuts at: 0 1090 1214 3759
Size: 1090 124 2545
Fragments arranged by size:
2545 1090 124
BssSI C'ACGA_G
Cuts at: 0 2893 3433 3759
Size: 2893 540 326
Fragments arranged by size:
2893 540 326
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1191 3759
Size: 1191 2568
BstEII G'GTnAC_C
Cuts at: 0 1467 3759
Size: 1467 2292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 1767 3759
Size: 639 1128 1992
Fragments arranged by size:
1992 1128 639
BstYI r'GATC_y
Cuts at: 0 597 1052 1904 1937 2668 2873 3296 3759
Size: 597 455 852 33 731 205 423 463
Fragments arranged by size:
852 731 597 463 455 423 205 33
Bsu36I CC'TnA_GG
Cuts at: 0 350 1474 3759
Size: 350 1124 2285
Fragments arranged by size:
2285 1124 350
BtgI C'CryG_G
Cuts at: 0 332 1652 1892 2780 3422 3759
Size: 332 1320 240 888 642 337
Fragments arranged by size:
1320 888 642 337 332 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 150 3204 3759
Size: 150 3054 555
Fragments arranged by size:
3054 555 150
BtsI GCAGTG_nn'
Cuts at: 0 543 1213 1447 1846 3457 3759
Size: 543 670 234 399 1611 302
Fragments arranged by size:
1611 670 543 399 302 234
ClaI AT'CG_AT
Cuts at: 0 1975 3759
Size: 1975 1784
DraIII CAC_nnn'GTG
Cuts at: 0 2493 3759
Size: 2493 1266
EaeI y'GGCC_r
Cuts at: 0 641 3759
Size: 641 3118
EarI CTCTTCn'nnn_
Cuts at: 0 698 1174 1292 3019 3759
Size: 698 476 118 1727 740
Fragments arranged by size:
1727 740 698 476 118
EcoICRI GAG'CTC
Cuts at: 0 708 2538 3759
Size: 708 1830 1221
Fragments arranged by size:
1830 1221 708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 70 238 276 364 541 889 1316
Size: 40 30 168 38 88 177 348 427
Cuts at: 1316 1647 1903 1978 2269 2679 2821 2938 2959
Size: 331 256 75 291 410 142 117 21
Cuts at: 2959 2986 3697 3759
Size: 27 711 62
Fragments arranged by size:
711 427 410 348 331 291 256 177
168 142 117 88 75 62 40 38
30 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 1354 2508 2992 3759
Size: 49 1305 1154 484 767
Fragments arranged by size:
1305 1154 767 484 49
EcoO109I rG'GnC_Cy
Cuts at: 0 1188 1611 1657 1756 2728 3200 3234 3759
Size: 1188 423 46 99 972 472 34 525
Fragments arranged by size:
1188 972 525 472 423 99 46 34
EcoRI G'AATT_C
Cuts at: 0 3620 3759
Size: 3620 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1641 1752 3759
Size: 1641 111 2007
Fragments arranged by size:
2007 1641 111
HaeII r_GCGC'y
Cuts at: 0 426 968 1183 1295 2024 2503 3255 3759
Size: 426 542 215 112 729 479 752 504
Fragments arranged by size:
752 729 542 504 479 426 215 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 302 334 442 474 589 621
Size: 16 32 254 32 108 32 115 32
Cuts at: 621 1252 1284 2195 2227 2362 2394 3759
Size: 631 32 911 32 135 32 1365
Fragments arranged by size:
1365 911 631 254 135 115 108 32
32 32 32 32 32 32 16
HindIII A'AGCT_T
Cuts at: 0 1243 3759
Size: 1243 2516
KasI G'GCGC_C
Cuts at: 0 422 3759
Size: 422 3337
KpnI G_GTAC'C
Cuts at: 0 2445 3759
Size: 2445 1314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 830 1295 2384 3759
Size: 59 771 465 1089 1375
Fragments arranged by size:
1375 1089 771 465 59
MscI TGG'CCA
Cuts at: 0 643 3759
Size: 643 3116
MslI CAynn'nnrTG
Cuts at: 0 496 1921 3358 3421 3703 3759
Size: 496 1425 1437 63 282 56
Fragments arranged by size:
1437 1425 496 282 63 56
MspA1I CmG'CkG
Cuts at: 0 1197 1485 1860 2937 3168 3513 3759
Size: 1197 288 375 1077 231 345 246
Fragments arranged by size:
1197 1077 375 345 288 246 231
NaeI GCC'GGC
Cuts at: 0 2755 3759
Size: 2755 1004
NarI GG'CG_CC
Cuts at: 0 423 3759
Size: 423 3336
NcoI C'CATG_G
Cuts at: 0 1892 3759
Size: 1892 1867
NgoMIV G'CCGG_C
Cuts at: 0 2753 3759
Size: 2753 1006
NheI G'CTAG_C
Cuts at: 0 2115 3759
Size: 2115 1644
NsiI A_TGCA'T
Cuts at: 0 153 3359 3759
Size: 153 3206 400
Fragments arranged by size:
3206 400 153
NspI r_CATG'y
Cuts at: 0 501 786 933 1071 2263 3361 3715 3759
Size: 501 285 147 138 1192 1098 354 44
Fragments arranged by size:
1192 1098 501 354 285 147 138 44
PciI A'CATG_T
Cuts at: 0 497 3759
Size: 497 3262
PflMI CCAn_nnn'nTGG
Cuts at: 0 1956 3759
Size: 1956 1803
PfoI T'CCnGG_A
Cuts at: 0 47 611 1237 1880 1955 2474 2915 3662
Size: 47 564 626 643 75 519 441 747
Cuts at: 3662 3759
Size: 97
Fragments arranged by size:
747 643 626 564 519 441 97 75
47
PmlI CAC'GTG
Cuts at: 0 1221 3759
Size: 1221 2538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1396 1428 3759
Size: 538 32 826 32 2331
Fragments arranged by size:
2331 826 538 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1611 1657 2728 3200 3234 3759
Size: 1611 46 1071 472 34 525
Fragments arranged by size:
1611 1071 525 472 46 34
PsiI TTA'TAA
Cuts at: 0 2141 3759
Size: 2141 1618
PspOMI G'GGCC_C
Cuts at: 0 1756 3759
Size: 1756 2003
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2674 2706 3759
Size: 2674 32 1053
Fragments arranged by size:
2674 1053 32
PstI C_TGCA'G
Cuts at: 0 674 1187 1196 1385 1454 1535 1838 2840
Size: 674 513 9 189 69 81 303 1002
Cuts at: 2840 3759
Size: 919
Fragments arranged by size:
1002 919 674 513 303 189 81 69
9
PvuII CAG'CTG
Cuts at: 0 1197 1860 2937 3759
Size: 1197 663 1077 822
Fragments arranged by size:
1197 1077 822 663
SacI G_AGCT'C
Cuts at: 0 710 2540 3759
Size: 710 1830 1219
Fragments arranged by size:
1830 1219 710
SanDI GG'GwC_CC
Cuts at: 0 1657 3200 3759
Size: 1657 1543 559
Fragments arranged by size:
1657 1543 559
SapI GCTCTTCn'nnn_
Cuts at: 0 698 1174 3759
Size: 698 476 2585
Fragments arranged by size:
2585 698 476
SbfI CC_TGCA'GG
Cuts at: 0 1385 1535 3759
Size: 1385 150 2224
Fragments arranged by size:
2224 1385 150
ScaI AGT'ACT
Cuts at: 0 892 1267 3759
Size: 892 375 2492
Fragments arranged by size:
2492 892 375
SexAI A'CCwGG_T
Cuts at: 0 2038 3759
Size: 2038 1721
SfcI C'TryA_G
Cuts at: 0 670 1095 1183 1192 1381 1450 1531 1834
Size: 670 425 88 9 189 69 81 303
Cuts at: 1834 2836 3759
Size: 1002 923
Fragments arranged by size:
1002 923 670 425 303 189 88 81
69 9
SfoI GGC'GCC
Cuts at: 0 424 3759
Size: 424 3335
SmaI CCC'GGG
Cuts at: 0 271 3759
Size: 271 3488
SmlI C'TyrA_G
Cuts at: 0 1737 1750 3759
Size: 1737 13 2009
Fragments arranged by size:
2009 1737 13
SnaBI TAC'GTA
Cuts at: 0 586 3759
Size: 586 3173
StuI AGG'CCT
Cuts at: 0 1379 3759
Size: 1379 2380
StyI C'CwwG_G
Cuts at: 0 1892 1964 2672 3759
Size: 1892 72 708 1087
Fragments arranged by size:
1892 1087 708 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2320 3759
Size: 2320 1439
TatI w'GTAC_w
Cuts at: 0 248 377 890 1025 1265 2009 3759
Size: 248 129 513 135 240 744 1750
Fragments arranged by size:
1750 744 513 248 240 135 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 593 625 3759
Size: 593 32 3134
Fragments arranged by size:
3134 593 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2388 3565 3759
Size: 2388 1177 194
Fragments arranged by size:
2388 1177 194
XmaI C'CCGG_G
Cuts at: 0 269 3759
Size: 269 3490
XmnI GAAnn'nnTTC
Cuts at: 0 451 1781 3759
Size: 451 1330 1978
Fragments arranged by size:
1978 1330 451
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmlI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII
SacI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI SnaBI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AscI AseI AsiSI AvrII BsiEI
BsiWI BspHI BstBI BstZ17I CspCI DraI DrdI EagI
EciI EcoRV FseI FspI FspAI HincII HpaI MfeI
MluI NdeI NotI NruI PacI PasI PmeI PshAI
PspXI PvuI RsrII SacII SalI SfiI SgfI SgrAI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI