(Linear) (Six-base) MAPSORT of: KIAA0069.seq  Check: 4581  from: 1  to: 609



KIAA0069.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     144     609 
   Size:        144     465
 
AhdI GACnn_n'nnGTC

Cuts at:      0      50     460     609 
   Size:         50     410     149

  Fragments arranged by size: 

                 410     149      50
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      46     609 
   Size:         46     563
 
AlwNI CAG_nnn'CTG

Cuts at:      0      46     609 
   Size:         46     563
 
ApoI r'AATT_y

Cuts at:      0     269     320     609 
   Size:        269      51     289

  Fragments arranged by size: 

                 289     269      51
 
BspHI T'CATG_A

Cuts at:      0     413     609 
   Size:        413     196
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      38     144     479     609 
   Size:         38     106     335     130

  Fragments arranged by size: 

                 335     130     106      38
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      46     609 
   Size:         46     563
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     609 
   Size:         33     576
 
BstYI r'GATC_y

Cuts at:      0     186     609 
   Size:        186     423
 
EaeI y'GGCC_r

Cuts at:      0     557     609 
   Size:        557      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     382     609 
   Size:        382     227
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     609 
   Size:         19     590
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     426     609 
   Size:        426     183
 
HaeII r_GCGC'y

Cuts at:      0     265     609 
   Size:        265     344
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      36      68     446     478     609 
   Size:         36      32     378      32     131

  Fragments arranged by size: 

                 378     131      36      32      32
 
MscI TGG'CCA

Cuts at:      0     559     609 
   Size:        559      50
 
MslI CAynn'nnrTG

Cuts at:      0     148     418     553     609 
   Size:        148     270     135      56

  Fragments arranged by size: 

                 270     148     135      56
 
MspA1I CmG'CkG

Cuts at:      0      66     609 
   Size:         66     543
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     287     609 
   Size:        287     322
 
PfoI T'CCnGG_A

Cuts at:      0     513     609 
   Size:        513      96
 
PstI C_TGCA'G

Cuts at:      0      42     609 
   Size:         42     567
 
PvuII CAG'CTG

Cuts at:      0      66     609 
   Size:         66     543
 
SfcI C'TryA_G

Cuts at:      0      38     609 
   Size:         38     571
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     136     438     609 
   Size:        136     302     171

  Fragments arranged by size: 

                 302     171     136
 
TatI w'GTAC_w

Cuts at:      0     407     545     609 
   Size:        407     138      64

  Fragments arranged by size: 

                 407     138      64
 
XbaI T'CTAG_A

Cuts at:      0     183     609 
   Size:        183     426
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     147     609 
   Size:        147     462
 

 Enzymes that do cut:

     AarI     AhdI     AlfI    AlwNI     ApoI    BspHI    BspMI   BstAPI
    BstXI    BstYI     EaeI     EarI     EciI     FalI    HaeII    Hin4I
     MscI     MslI   MspA1I    PflMI     PfoI     PstI    PvuII     SfcI
    TaqII     TatI     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI