Restriction map for
pF1KSDA0070
(Linear) (Six-base) MAPSORT of: KIAA0070.seq Check: 5950 from: 1 to: 1791
KIAA0070.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 350 1552 1791
Size: 350 1202 239
Fragments arranged by size:
1202 350 239
AccI GT'mk_AC
Cuts at: 0 302 1495 1791
Size: 302 1193 296
Fragments arranged by size:
1193 302 296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 280 1268 1791
Size: 88 192 988 523
Fragments arranged by size:
988 523 192 88
AfeI AGC'GCT
Cuts at: 0 1453 1791
Size: 1453 338
ApoI r'AATT_y
Cuts at: 0 502 727 1262 1791
Size: 502 225 535 529
Fragments arranged by size:
535 529 502 225
AseI AT'TA_AT
Cuts at: 0 515 1791
Size: 515 1276
BamHI G'GATC_C
Cuts at: 0 392 1791
Size: 392 1399
BanI G'GyrC_C
Cuts at: 0 1424 1791
Size: 1424 367
BanII G_rGCy'C
Cuts at: 0 128 1411 1791
Size: 128 1283 380
Fragments arranged by size:
1283 380 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 281 1733 1791
Size: 281 1452 58
Fragments arranged by size:
1452 281 58
BclI T'GATC_A
Cuts at: 0 1389 1791
Size: 1389 402
BglI GCCn_nnn'nGGC
Cuts at: 0 13 1136 1791
Size: 13 1123 655
Fragments arranged by size:
1123 655 13
BlpI GC'TnA_GC
Cuts at: 0 142 586 1791
Size: 142 444 1205
Fragments arranged by size:
1205 444 142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 911 1791
Size: 911 880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1791
Size: 881 910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 1791
Size: 1219 572
BsaBI GATnn'nnATC
Cuts at: 0 814 1791
Size: 814 977
BsaHI Gr'CG_yC
Cuts at: 0 75 957 1791
Size: 75 882 834
Fragments arranged by size:
882 834 75
BsaWI w'CCGG_w
Cuts at: 0 965 1791
Size: 965 826
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 1791
Size: 437 30 1324
Fragments arranged by size:
1324 437 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 458 1791
Size: 458 1333
BseYI C'CCAG_C
Cuts at: 0 780 1637 1791
Size: 780 857 154
Fragments arranged by size:
857 780 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1791
Size: 31 1760
BsiEI CG_ry'CG
Cuts at: 0 8 20 939 1791
Size: 8 12 919 852
Fragments arranged by size:
919 852 12 8
BsiHKAI G_wGCw'C
Cuts at: 0 128 1791
Size: 128 1663
BsmI GAATG_Cn'
Cuts at: 0 1303 1791
Size: 1303 488
BsmBI CGTCTCn'nnnn_
Cuts at: 0 66 1791
Size: 66 1725
Bsp1286I G_dGCh'C
Cuts at: 0 128 1411 1791
Size: 128 1283 380
Fragments arranged by size:
1283 380 128
BspEI T'CCGG_A
Cuts at: 0 965 1791
Size: 965 826
BspHI T'CATG_A
Cuts at: 0 1469 1791
Size: 1469 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 376 1552 1791
Size: 350 26 1176 239
Fragments arranged by size:
1176 350 239 26
BsrBI CCG'CTC
Cuts at: 0 740 1430 1791
Size: 740 690 361
Fragments arranged by size:
740 690 361
BsrDI GCAATG_nn'
Cuts at: 0 1494 1791
Size: 1494 297
BstEII G'GTnAC_C
Cuts at: 0 1110 1791
Size: 1110 681
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1126 1791
Size: 1126 665
BstYI r'GATC_y
Cuts at: 0 392 1791
Size: 392 1399
BstZ17I GTA'TAC
Cuts at: 0 1496 1791
Size: 1496 295
Bsu36I CC'TnA_GG
Cuts at: 0 197 1791
Size: 197 1594
BtgI C'CryG_G
Cuts at: 0 833 1791
Size: 833 958
BtsI GCAGTG_nn'
Cuts at: 0 152 610 1791
Size: 152 458 1181
Fragments arranged by size:
1181 458 152
EaeI y'GGCC_r
Cuts at: 0 5 17 470 836 1791
Size: 5 12 453 366 955
Fragments arranged by size:
955 453 366 12 5
EagI C'GGCC_G
Cuts at: 0 5 17 1791
Size: 5 12 1774
Fragments arranged by size:
1774 12 5
EarI CTCTTCn'nnn_
Cuts at: 0 64 197 1187 1791
Size: 64 133 990 604
Fragments arranged by size:
990 604 133 64
EcoICRI GAG'CTC
Cuts at: 0 126 1791
Size: 126 1665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 280 881 1268 1791
Size: 88 192 601 387 523
Fragments arranged by size:
601 523 387 192 88
EcoO109I rG'GnC_Cy
Cuts at: 0 193 1791
Size: 193 1598
EcoRV GAT'ATC
Cuts at: 0 1275 1791
Size: 1275 516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 444 1791
Size: 444 1347
HaeII r_GCGC'y
Cuts at: 0 1455 1791
Size: 1455 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 786 818 978 1010 1791
Size: 786 32 160 32 781
Fragments arranged by size:
786 781 160 32 32
HincII GTy'rAC
Cuts at: 0 1155 1791
Size: 1155 636
HindIII A'AGCT_T
Cuts at: 0 1336 1791
Size: 1336 455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 1791
Size: 1706 85
MscI TGG'CCA
Cuts at: 0 472 838 1791
Size: 472 366 953
Fragments arranged by size:
953 472 366
MslI CAynn'nnrTG
Cuts at: 0 1124 1791
Size: 1124 667
MspA1I CmG'CkG
Cuts at: 0 258 1524 1633 1791
Size: 258 1266 109 158
Fragments arranged by size:
1266 258 158 109
NdeI CA'TA_TG
Cuts at: 0 632 1791
Size: 632 1159
PasI CC'CwG_GG
Cuts at: 0 823 1209 1791
Size: 823 386 582
Fragments arranged by size:
823 582 386
PfoI T'CCnGG_A
Cuts at: 0 1352 1791
Size: 1352 439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 1791
Size: 1564 32 195
Fragments arranged by size:
1564 195 32
PpuMI rG'GwC_Cy
Cuts at: 0 193 1791
Size: 193 1598
PsiI TTA'TAA
Cuts at: 0 488 1791
Size: 488 1303
PstI C_TGCA'G
Cuts at: 0 347 1563 1791
Size: 347 1216 228
Fragments arranged by size:
1216 347 228
PvuII CAG'CTG
Cuts at: 0 258 1633 1791
Size: 258 1375 158
Fragments arranged by size:
1375 258 158
SacI G_AGCT'C
Cuts at: 0 128 1791
Size: 128 1663
SapI GCTCTTCn'nnn_
Cuts at: 0 1187 1791
Size: 1187 604
ScaI AGT'ACT
Cuts at: 0 1700 1791
Size: 1700 91
SfcI C'TryA_G
Cuts at: 0 343 1559 1791
Size: 343 1216 232
Fragments arranged by size:
1216 343 232
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1136 1791
Size: 1136 655
SmlI C'TyrA_G
Cuts at: 0 1198 1791
Size: 1198 593
StyI C'CwwG_G
Cuts at: 0 1547 1553 1791
Size: 1547 6 238
Fragments arranged by size:
1547 238 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1136 1409 1791
Size: 1136 273 382
Fragments arranged by size:
1136 382 273
TatI w'GTAC_w
Cuts at: 0 1600 1698 1791
Size: 1600 98 93
Fragments arranged by size:
1600 98 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 437 469 1791
Size: 278 32 127 32 1322
Fragments arranged by size:
1322 278 127 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1758 1791
Size: 1758 33
XmnI GAAnn'nnTTC
Cuts at: 0 506 1700 1791
Size: 506 1194 91
Fragments arranged by size:
1194 506 91
Enzymes that do cut:
AarI AccI AcuI AfeI ApoI AseI BamHI BanI
BanII BbsI BclI BglI BlpI BplI BpmI BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NdeI PasI PfoI PpiI PpuMI PsiI PstI PvuII
SacI SapI ScaI SfcI SfiI SmlI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII
Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsiWI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoRI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI