Restriction map for
pF1KSDA0076
(Linear) (Six-base) MAPSORT of: KIAA0076.seq Check: 4697 from: 1 to: 5094
KIAA0076.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3302 3450 5094
Size: 3302 148 1644
Fragments arranged by size:
3302 1644 148
AatII G_ACGT'C
Cuts at: 0 4862 5094
Size: 4862 232
AccI GT'mk_AC
Cuts at: 0 1691 3959 5094
Size: 1691 2268 1135
Fragments arranged by size:
2268 1691 1135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1730 2089 3637 4456 5094
Size: 1035 695 359 1548 819 638
Fragments arranged by size:
1548 1035 819 695 638 359
AfeI AGC'GCT
Cuts at: 0 2851 5094
Size: 2851 2243
AflIII A'CryG_T
Cuts at: 0 503 5094
Size: 503 4591
AleI CACnn'nnGTG
Cuts at: 0 3647 5094
Size: 3647 1447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3086 5094
Size: 3086 2008
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 2311 2343 3148 3180 4258 4290
Size: 868 32 1411 32 805 32 1078 32
Cuts at: 4290 4972 5004 5094
Size: 682 32 90
Fragments arranged by size:
1411 1078 868 805 682 90 32 32
32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 653 1869 2016 2181 2313 3216 3921 3975
Size: 653 1216 147 165 132 903 705 54
Cuts at: 3975 4235 4386 4929 4945 5094
Size: 260 151 543 16 149
Fragments arranged by size:
1216 903 705 653 543 260 165 151
149 147 132 54 16
ApaI G_GGCC'C
Cuts at: 0 47 272 303 4511 4798 5094
Size: 47 225 31 4208 287 296
Fragments arranged by size:
4208 296 287 225 47 31
ApaLI G'TGCA_C
Cuts at: 0 1267 5094
Size: 1267 3827
ApoI r'AATT_y
Cuts at: 0 25 3066 5094
Size: 25 3041 2028
Fragments arranged by size:
3041 2028 25
AvaI C'yCGr_G
Cuts at: 0 2089 2691 2708 3395 3566 3840 5039 5094
Size: 2089 602 17 687 171 274 1199 55
Fragments arranged by size:
2089 1199 687 602 274 171 55 17
AvrII C'CTAG_G
Cuts at: 0 2898 5094
Size: 2898 2196
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4405 4438 5094
Size: 4405 33 656
Fragments arranged by size:
4405 656 33
BanI G'GyrC_C
Cuts at: 0 33 901 3321 5094
Size: 33 868 2420 1773
Fragments arranged by size:
2420 1773 868 33
BanII G_rGCy'C
Cuts at: 0 47 163 272 303 363 455 828 967
Size: 47 116 109 31 60 92 373 139
Cuts at: 967 1018 1025 1374 2491 3314 3385 3708 4478
Size: 51 7 349 1117 823 71 323 770
Cuts at: 4478 4511 4798 5094
Size: 33 287 296
Fragments arranged by size:
1117 823 770 373 349 323 296 287
139 116 109 92 71 60 51 47
33 31 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1310 2423 3228 4670 5094
Size: 1310 1113 805 1442 424
Fragments arranged by size:
1442 1310 1113 805 424
BbvCI CC'TCA_GC
Cuts at: 0 998 1924 4655 5094
Size: 998 926 2731 439
Fragments arranged by size:
2731 998 926 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 793 827 2682 2716 2926 2960 3678 3712
Size: 793 34 1855 34 210 34 718 34
Cuts at: 3712 5094
Size: 1382
Fragments arranged by size:
1855 1382 793 718 210 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3996 5094
Size: 3996 1098
BclI T'GATC_A
Cuts at: 0 1676 5094
Size: 1676 3418
BglI GCCn_nnn'nGGC
Cuts at: 0 42 1383 2134 5094
Size: 42 1341 751 2960
Fragments arranged by size:
2960 1341 751 42
BglII A'GATC_T
Cuts at: 0 218 1286 5094
Size: 218 1068 3808
Fragments arranged by size:
3808 1068 218
BlpI GC'TnA_GC
Cuts at: 0 185 430 966 1040 4361 5094
Size: 185 245 536 74 3321 733
Fragments arranged by size:
3321 733 536 245 185 74
Bme1580I G_kGCm'C
Cuts at: 0 38 47 272 303 391 904 1271 1607
Size: 38 9 225 31 88 513 367 336
Cuts at: 1607 1665 2979 4261 4511 4798 4897 5051 5094
Size: 58 1314 1282 250 287 99 154 43
Fragments arranged by size:
1314 1282 513 367 336 287 250 225
154 99 88 58 43 38 31 9
BmgBI CAC'GTC
Cuts at: 0 3957 4346 5094
Size: 3957 389 748
Fragments arranged by size:
3957 748 389
BmtI G_CTAG'C
Cuts at: 0 667 5094
Size: 667 4427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2188 2556 4338 4588 5094
Size: 2188 368 1782 250 506
Fragments arranged by size:
2188 1782 506 368 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 394 482 555 895 901 1303 1410
Size: 340 54 88 73 340 6 402 107
Cuts at: 1410 1522 1531 2158 2317 2475 2824 3006 3238
Size: 112 9 627 159 158 349 182 232
Cuts at: 3238 3399 3871 3886 3952 4018 4798 5094
Size: 161 472 15 66 66 780 296
Fragments arranged by size:
780 627 472 402 349 340 340 296
232 182 161 159 158 112 107 88
73 66 66 54 15 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 998 1793 1924 2306 3922 4655 5094
Size: 998 795 131 382 1616 733 439
Fragments arranged by size:
1616 998 795 733 439 382 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3528 4333 4558 4708 5094
Size: 3528 805 225 150 386
Fragments arranged by size:
3528 805 386 225 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 3843 4449 5094
Size: 3843 606 645
Fragments arranged by size:
3843 645 606
BsaAI yAC'GTr
Cuts at: 0 426 3645 5094
Size: 426 3219 1449
Fragments arranged by size:
3219 1449 426
BsaBI GATnn'nnATC
Cuts at: 0 101 5094
Size: 101 4993
BsaHI Gr'CG_yC
Cuts at: 0 4859 4905 5094
Size: 4859 46 189
Fragments arranged by size:
4859 189 46
BsaWI w'CCGG_w
Cuts at: 0 2025 5094
Size: 2025 3069
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 381 411 825 855 868 898 2087 2117
Size: 381 30 414 30 13 30 1189 30
Cuts at: 2117 2248 2278 2500 2530 3148 3178 3367 3397
Size: 131 30 222 30 618 30 189 30
Cuts at: 3397 4258 4288 4849 4879 4972 5002 5094
Size: 861 30 561 30 93 30 92
Fragments arranged by size:
1189 861 618 561 414 381 222 189
131 93 92 30 30 30 30 30
30 30 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 391 395 862 1183 1465 1480 3335
Size: 337 54 4 467 321 282 15 1855
Cuts at: 3335 4126 4135 4138 4980 5094
Size: 791 9 3 842 114
Fragments arranged by size:
1855 842 791 467 337 321 282 114
54 15 9 4 3
BseYI C'CCAG_C
Cuts at: 0 243 577 935 1891 2244 2403 2519 3125
Size: 243 334 358 956 353 159 116 606
Cuts at: 3125 3258 3902 4075 4350 4355 5094
Size: 133 644 173 275 5 739
Fragments arranged by size:
956 739 644 606 358 353 334 275
243 173 159 133 116 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1249 2029 2179 2617 3007 3172 4423
Size: 560 689 780 150 438 390 165 1251
Cuts at: 4423 4732 5046 5094
Size: 309 314 48
Fragments arranged by size:
1251 780 689 560 438 390 314 309
165 150 48
BsiEI CG_ry'CG
Cuts at: 0 4917 5094
Size: 4917 177
BsiHKAI G_wGCw'C
Cuts at: 0 191 431 660 749 967 1271 2174 3051
Size: 191 240 229 89 218 304 903 877
Cuts at: 3051 3385 3524 3928 4052 5094
Size: 334 139 404 124 1042
Fragments arranged by size:
1042 903 877 404 334 304 240 229
218 191 139 124 89
BsmI GAATG_Cn'
Cuts at: 0 716 2879 5094
Size: 716 2163 2215
Fragments arranged by size:
2215 2163 716
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2249 3289 3829 4680 4896 5094
Size: 2249 1040 540 851 216 198
Fragments arranged by size:
2249 1040 851 540 216 198
Bsp1286I G_dGCh'C
Cuts at: 0 38 47 163 191 272 303 363 391
Size: 38 9 116 28 81 31 60 28
Cuts at: 391 431 455 660 749 828 904 967 1018
Size: 40 24 205 89 79 76 63 51
Cuts at: 1018 1025 1271 1374 1607 1665 2174 2491 2979
Size: 7 246 103 233 58 509 317 488
Cuts at: 2979 3051 3314 3385 3524 3708 3928 4052 4261
Size: 72 263 71 139 184 220 124 209
Cuts at: 4261 4478 4511 4798 4897 5051 5094
Size: 217 33 287 99 154 43
Fragments arranged by size:
509 488 317 287 263 246 233 220
217 209 205 184 154 139 124 116
103 99 89 81 79 76 72 71
63 60 58 51 43 40 38 33
31 28 28 24 9 7
BspEI T'CCGG_A
Cuts at: 0 2025 5094
Size: 2025 3069
BspHI T'CATG_A
Cuts at: 0 3416 5094
Size: 3416 1678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 3130 3302 3450 4243 4655 5094
Size: 1221 1909 172 148 793 412 439
Fragments arranged by size:
1909 1221 793 439 412 172 148
BsrDI GCAATG_nn'
Cuts at: 0 3745 4160 5094
Size: 3745 415 934
Fragments arranged by size:
3745 934 415
BsrFI r'CCGG_y
Cuts at: 0 2435 2611 2703 4916 5094
Size: 2435 176 92 2213 178
Fragments arranged by size:
2435 2213 178 176 92
BssSI C'ACGA_G
Cuts at: 0 2113 4543 5094
Size: 2113 2430 551
Fragments arranged by size:
2430 2113 551
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 673 930 2138 3122 3744 3912 5094
Size: 195 478 257 1208 984 622 168 1182
Fragments arranged by size:
1208 1182 984 622 478 257 195 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 577 1614 2274 2379 2863 4815 5094
Size: 577 1037 660 105 484 1952 279
Fragments arranged by size:
1952 1037 660 577 484 279 105
BstYI r'GATC_y
Cuts at: 0 116 218 593 1286 1541 1583 1908 2837
Size: 116 102 375 693 255 42 325 929
Cuts at: 2837 2894 3680 5094
Size: 57 786 1414
Fragments arranged by size:
1414 929 786 693 375 325 255 116
102 57 42
Bsu36I CC'TnA_GG
Cuts at: 0 845 1433 1666 4252 4992 5094
Size: 845 588 233 2586 740 102
Fragments arranged by size:
2586 845 740 588 233 102
BtgI C'CryG_G
Cuts at: 0 896 2120 2228 3146 3252 3446 4731 5094
Size: 896 1224 108 918 106 194 1285 363
Fragments arranged by size:
1285 1224 918 896 363 194 108 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 158 5094
Size: 158 4936
BtsI GCAGTG_nn'
Cuts at: 0 1126 1352 2095 4094 4345 5063 5094
Size: 1126 226 743 1999 251 718 31
Fragments arranged by size:
1999 1126 743 718 251 226 31
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3771 3806 5094
Size: 3771 35 1288
Fragments arranged by size:
3771 1288 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 481 4762 5094
Size: 481 4281 332
Fragments arranged by size:
4281 481 332
EaeI y'GGCC_r
Cuts at: 0 165 255 893 1349 1598 2920 3338 4668
Size: 165 90 638 456 249 1322 418 1330
Cuts at: 4668 5094
Size: 426
Fragments arranged by size:
1330 1322 638 456 426 418 249 165
90
EarI CTCTTCn'nnn_
Cuts at: 0 754 1498 2750 2965 4061 4112 4524 4678
Size: 754 744 1252 215 1096 51 412 154
Cuts at: 4678 5094
Size: 416
Fragments arranged by size:
1252 1096 754 744 416 412 215 154
51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 3832 4117 5094
Size: 67 3765 285 977
Fragments arranged by size:
3765 977 285 67
EcoICRI GAG'CTC
Cuts at: 0 965 3383 5094
Size: 965 2418 1711
Fragments arranged by size:
2418 1711 965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 394 482 555 895 901 1035 1303
Size: 340 54 88 73 340 6 134 268
Cuts at: 1303 1410 1522 1531 1730 2089 2158 2317 2475
Size: 107 112 9 199 359 69 159 158
Cuts at: 2475 2824 3006 3238 3399 3637 3871 3886 3952
Size: 349 182 232 161 238 234 15 66
Cuts at: 3952 4018 4456 4798 5094
Size: 66 438 342 296
Fragments arranged by size:
438 359 349 342 340 340 296 268
238 234 232 199 182 161 159 158
134 112 107 88 73 69 66 66
54 15 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1050 1053 2482 4251 4831 4888 5094
Size: 1050 3 1429 1769 580 57 206
Fragments arranged by size:
1769 1429 1050 580 206 57 3
EcoO109I rG'GnC_Cy
Cuts at: 0 43 269 299 300 474 483 581 732
Size: 43 226 30 1 174 9 98 151
Cuts at: 732 1671 1937 2039 2271 2902 3132 3399 4134
Size: 939 266 102 232 631 230 267 735
Cuts at: 4134 4508 4528 4569 4794 4996 5094
Size: 374 20 41 225 202 98
Fragments arranged by size:
939 735 631 374 267 266 232 230
226 225 202 174 151 102 98 98
43 41 30 20 9 1
EcoRI G'AATT_C
Cuts at: 0 25 5094
Size: 25 5069
HaeII r_GCGC'y
Cuts at: 0 437 1389 2612 2853 3589 3948 5094
Size: 437 952 1223 241 736 359 1146
Fragments arranged by size:
1223 1146 952 736 437 359 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 328 360 573 605 931 963
Size: 145 32 151 32 213 32 326 32
Cuts at: 963 2246 2248 2278 2280 2375 2407 3367 3399
Size: 1283 2 30 2 95 32 960 32
Cuts at: 3399 4677 4709 4849 4881 5094
Size: 1278 32 140 32 213
Fragments arranged by size:
1283 1278 960 326 213 213 151 145
140 95 32 32 32 32 32 32
32 32 30 2 2
HincII GTy'rAC
Cuts at: 0 871 1247 5094
Size: 871 376 3847
Fragments arranged by size:
3847 871 376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2567 2624 4301 5094
Size: 2567 57 1677 793
Fragments arranged by size:
2567 1677 793 57
MscI TGG'CCA
Cuts at: 0 167 257 895 1351 2922 3340 5094
Size: 167 90 638 456 1571 418 1754
Fragments arranged by size:
1754 1571 638 456 418 167 90
MslI CAynn'nnrTG
Cuts at: 0 502 2395 3647 4736 5094
Size: 502 1893 1252 1089 358
Fragments arranged by size:
1893 1252 1089 502 358
MspA1I CmG'CkG
Cuts at: 0 372 653 798 1002 1030 1891 1974 1980
Size: 372 281 145 204 28 861 83 6
Cuts at: 1980 2076 2244 3254 3732 3975 5094
Size: 96 168 1010 478 243 1119
Fragments arranged by size:
1119 1010 861 478 372 281 243 204
168 145 96 83 28 6
NaeI GCC'GGC
Cuts at: 0 2613 2705 5094
Size: 2613 92 2389
Fragments arranged by size:
2613 2389 92
NcoI C'CATG_G
Cuts at: 0 896 4731 5094
Size: 896 3835 363
Fragments arranged by size:
3835 896 363
NgoMIV G'CCGG_C
Cuts at: 0 2611 2703 5094
Size: 2611 92 2391
Fragments arranged by size:
2611 2391 92
NheI G'CTAG_C
Cuts at: 0 663 5094
Size: 663 4431
NspI r_CATG'y
Cuts at: 0 58 507 778 1281 2347 2640 2703 3307
Size: 58 449 271 503 1066 293 63 604
Cuts at: 3307 4393 4491 4847 5094
Size: 1086 98 356 247
Fragments arranged by size:
1086 1066 604 503 449 356 293 271
247 98 63 58
PasI CC'CwG_GG
Cuts at: 0 39 1018 3192 4808 5094
Size: 39 979 2174 1616 286
Fragments arranged by size:
2174 1616 979 286 39
PciI A'CATG_T
Cuts at: 0 503 5094
Size: 503 4591
PflMI CCAn_nnn'nTGG
Cuts at: 0 87 935 2918 2997 3111 3216 3714 3975
Size: 87 848 1983 79 114 105 498 261
Cuts at: 3975 4677 5094
Size: 702 417
Fragments arranged by size:
1983 848 702 498 417 261 114 105
87 79
PfoI T'CCnGG_A
Cuts at: 0 485 788 1547 1904 2174 2801 2930 5094
Size: 485 303 759 357 270 627 129 2164
Fragments arranged by size:
2164 759 627 485 357 303 270 129
PmlI CAC'GTG
Cuts at: 0 426 3645 5094
Size: 426 3219 1449
Fragments arranged by size:
3219 1449 426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 868 900 3148 3180 3532 3564 4258 4290
Size: 868 32 2248 32 352 32 694 32
Cuts at: 4290 4972 5004 5094
Size: 682 32 90
Fragments arranged by size:
2248 868 694 682 352 90 32 32
32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 474 483 581 732 1671 1937 2039 4134
Size: 474 9 98 151 939 266 102 2095
Cuts at: 4134 4569 5094
Size: 435 525
Fragments arranged by size:
2095 939 525 474 435 266 151 102
98 9
PspOMI G'GGCC_C
Cuts at: 0 43 268 299 4507 4794 5094
Size: 43 225 31 4208 287 300
Fragments arranged by size:
4208 300 287 225 43 31
PspXI vC'TCGA_Gb
Cuts at: 0 2691 5094
Size: 2691 2403
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1717 1749 5094
Size: 1717 32 3345
Fragments arranged by size:
3345 1717 32
PstI C_TGCA'G
Cuts at: 0 288 1133 1865 1973 1979 3539 3737 3944
Size: 288 845 732 108 6 1560 198 207
Cuts at: 3944 4340 4370 5094
Size: 396 30 724
Fragments arranged by size:
1560 845 732 724 396 288 207 198
108 30 6
PvuII CAG'CTG
Cuts at: 0 372 798 1002 1891 1974 2076 2244 3732
Size: 372 426 204 889 83 102 168 1488
Cuts at: 3732 3975 5094
Size: 243 1119
Fragments arranged by size:
1488 1119 889 426 372 243 204 168
102 83
RsrII CG'GwC_CG
Cuts at: 0 3819 5094
Size: 3819 1275
SacI G_AGCT'C
Cuts at: 0 967 3385 5094
Size: 967 2418 1709
Fragments arranged by size:
2418 1709 967
SacII CC_GC'GG
Cuts at: 0 3255 5094
Size: 3255 1839
SanDI GG'GwC_CC
Cuts at: 0 581 1937 5094
Size: 581 1356 3157
Fragments arranged by size:
3157 1356 581
SapI GCTCTTCn'nnn_
Cuts at: 0 754 5094
Size: 754 4340
SfcI C'TryA_G
Cuts at: 0 18 284 1129 1861 1969 1975 3535 3733
Size: 18 266 845 732 108 6 1560 198
Cuts at: 3733 3940 4336 4366 5026 5094
Size: 207 396 30 660 68
Fragments arranged by size:
1560 845 732 660 396 266 207 198
108 68 30 18 6
SmaI CCC'GGG
Cuts at: 0 3397 5041 5094
Size: 3397 1644 53
Fragments arranged by size:
3397 1644 53
SmlI C'TyrA_G
Cuts at: 0 2691 3543 3840 4312 4573 4723 5094
Size: 2691 852 297 472 261 150 371
Fragments arranged by size:
2691 852 472 371 297 261 150
SphI G_CATG'C
Cuts at: 0 2347 4847 5094
Size: 2347 2500 247
Fragments arranged by size:
2500 2347 247
SspI AAT'ATT
Cuts at: 0 3531 5094
Size: 3531 1563
StuI AGG'CCT
Cuts at: 0 946 2983 5094
Size: 946 2037 2111
Fragments arranged by size:
2111 2037 946
StyI C'CwwG_G
Cuts at: 0 209 478 896 958 1338 2267 2898 3386
Size: 209 269 418 62 380 929 631 488
Cuts at: 3386 4029 4731 4827 5094
Size: 643 702 96 267
Fragments arranged by size:
929 702 643 631 488 418 380 269
267 209 96 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1254 3865 5086 5094
Size: 1254 2611 1221 8
Fragments arranged by size:
2611 1254 1221 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 381 413 825 857 2087 2119 2500 2532
Size: 381 32 412 32 1230 32 381 32
Cuts at: 2532 4957 4989 5094
Size: 2425 32 105
Fragments arranged by size:
2425 1230 412 381 381 105 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 1746 1911 3034 5094
Size: 1746 165 1123 2060
Fragments arranged by size:
2060 1746 1123 165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1359 5094
Size: 1359 3735
XhoI C'TCGA_G
Cuts at: 0 2691 3840 5094
Size: 2691 1149 1254
Fragments arranged by size:
2691 1254 1149
XmaI C'CCGG_G
Cuts at: 0 3395 5039 5094
Size: 3395 1644 55
Fragments arranged by size:
3395 1644 55
ZraI GAC'GTC
Cuts at: 0 4860 5094
Size: 4860 234
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuII RsrII SacI
SacII SanDI SapI SfcI SmaI SmlI SphI SspI
StuI StyI TaqII TstI XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AscI AseI AsiSI
BamHI BbeI BfrBI BmrI BsiWI BsrBI BsrGI BssHII
BstBI BstEII BstZ17I ClaI DraI DraIII EagI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NotI NruI NsiI PacI
PmeI PshAI PsiI PvuI SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI
TatI Tth111I XmnI