Restriction map for
pF1KA0078
(Linear) (Six-base) MAPSORT of: KIAA0078.seq Check: 5462 from: 1 to: 1893
KIAA0078.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1177 1684 1893
Size: 353 824 507 209
Fragments arranged by size:
824 507 353 209
AflIII A'CryG_T
Cuts at: 0 542 1893
Size: 542 1351
AlwNI CAG_nnn'CTG
Cuts at: 0 360 1459 1893
Size: 360 1099 434
Fragments arranged by size:
1099 434 360
ApoI r'AATT_y
Cuts at: 0 47 337 1243 1255 1893
Size: 47 290 906 12 638
Fragments arranged by size:
906 638 290 47 12
BamHI G'GATC_C
Cuts at: 0 843 1893
Size: 843 1050
BanI G'GyrC_C
Cuts at: 0 1059 1893
Size: 1059 834
BanII G_rGCy'C
Cuts at: 0 1017 1328 1893
Size: 1017 311 565
Fragments arranged by size:
1017 565 311
BbsI GAAGACnn'nnnn_
Cuts at: 0 1202 1690 1893
Size: 1202 488 203
Fragments arranged by size:
1202 488 203
BbvCI CC'TCA_GC
Cuts at: 0 1472 1893
Size: 1472 421
BclI T'GATC_A
Cuts at: 0 351 873 1893
Size: 351 522 1020
Fragments arranged by size:
1020 522 351
BfrBI ATG'CAT
Cuts at: 0 1630 1893
Size: 1630 263
BlpI GC'TnA_GC
Cuts at: 0 1130 1893
Size: 1130 763
BmrI ACTGGGnnnn_n'
Cuts at: 0 190 1893
Size: 190 1703
BmtI G_CTAG'C
Cuts at: 0 58 1893
Size: 58 1835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1742 1893
Size: 1336 406 151
Fragments arranged by size:
1336 406 151
Bpu10I CC'TnA_GC
Cuts at: 0 1472 1893
Size: 1472 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1765 1893
Size: 1765 128
BsaBI GATnn'nnATC
Cuts at: 0 1646 1893
Size: 1646 247
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1115 1235 1460 1893
Size: 1115 120 225 433
Fragments arranged by size:
1115 433 225 120
BseYI C'CCAG_C
Cuts at: 0 389 1893
Size: 389 1504
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1893
Size: 761 1132
BsiHKAI G_wGCw'C
Cuts at: 0 595 1710 1893
Size: 595 1115 183
Fragments arranged by size:
1115 595 183
BsmI GAATG_Cn'
Cuts at: 0 241 1893
Size: 241 1652
Bsp1286I G_dGCh'C
Cuts at: 0 595 1017 1328 1710 1893
Size: 595 422 311 382 183
Fragments arranged by size:
595 422 382 311 183
BspHI T'CATG_A
Cuts at: 0 342 1893
Size: 342 1551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 1134 1467 1893
Size: 293 841 333 426
Fragments arranged by size:
841 426 333 293
BstBI TT'CG_AA
Cuts at: 0 192 1893
Size: 192 1701
BstYI r'GATC_y
Cuts at: 0 843 1053 1893
Size: 843 210 840
Fragments arranged by size:
843 840 210
Bsu36I CC'TnA_GG
Cuts at: 0 290 1418 1893
Size: 290 1128 475
Fragments arranged by size:
1128 475 290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1845 1893
Size: 1845 48
BtsI GCAGTG_nn'
Cuts at: 0 358 528 1893
Size: 358 170 1365
Fragments arranged by size:
1365 358 170
ClaI AT'CG_AT
Cuts at: 0 380 1319 1893
Size: 380 939 574
Fragments arranged by size:
939 574 380
EaeI y'GGCC_r
Cuts at: 0 41 268 1893
Size: 41 227 1625
Fragments arranged by size:
1625 227 41
EarI CTCTTCn'nnn_
Cuts at: 0 416 955 1331 1604 1610 1893
Size: 416 539 376 273 6 283
Fragments arranged by size:
539 416 376 283 273 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1177 1336 1684 1742 1893
Size: 353 824 159 348 58 151
Fragments arranged by size:
824 353 348 159 151 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 745 1893
Size: 289 456 1148
Fragments arranged by size:
1148 456 289
EcoO109I rG'GnC_Cy
Cuts at: 0 34 1893
Size: 34 1859
EcoRI G'AATT_C
Cuts at: 0 1243 1893
Size: 1243 650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 793 825 1455 1487 1893
Size: 793 32 630 32 406
Fragments arranged by size:
793 630 406 32 32
HincII GTy'rAC
Cuts at: 0 282 978 1893
Size: 282 696 915
Fragments arranged by size:
915 696 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1893
Size: 974 919
MscI TGG'CCA
Cuts at: 0 43 1893
Size: 43 1850
MslI CAynn'nnrTG
Cuts at: 0 382 1893
Size: 382 1511
NheI G'CTAG_C
Cuts at: 0 54 1893
Size: 54 1839
NsiI A_TGCA'T
Cuts at: 0 1632 1893
Size: 1632 261
NspI r_CATG'y
Cuts at: 0 546 1893
Size: 546 1347
PciI A'CATG_T
Cuts at: 0 542 1893
Size: 542 1351
PflMI CCAn_nnn'nTGG
Cuts at: 0 50 1893
Size: 50 1843
PfoI T'CCnGG_A
Cuts at: 0 1352 1893
Size: 1352 541
PsiI TTA'TAA
Cuts at: 0 314 1893
Size: 314 1579
SapI GCTCTTCn'nnn_
Cuts at: 0 1331 1893
Size: 1331 562
SfcI C'TryA_G
Cuts at: 0 1502 1791 1893
Size: 1502 289 102
Fragments arranged by size:
1502 289 102
SmlI C'TyrA_G
Cuts at: 0 1744 1893
Size: 1744 149
SspI AAT'ATT
Cuts at: 0 683 1893
Size: 683 1210
StyI C'CwwG_G
Cuts at: 0 1876 1893
Size: 1876 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1893
Size: 805 1088
Tth111I GACn'n_nGTC
Cuts at: 0 982 1893
Size: 982 911
XmnI GAAnn'nnTTC
Cuts at: 0 338 1893
Size: 338 1555
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BamHI BanI BanII BbsI
BbvCI BclI BfrBI BlpI BmrI BmtI BpmI Bpu10I
BpuEI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BstBI BstYI Bsu36I BtgZI BtsI ClaI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
MmeI MscI MslI NheI NsiI NspI PciI PflMI
PfoI PsiI SapI SfcI SmlI SspI StyI TaqII
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI
BglII Bme1580I BmgBI BplI BsaI BsaAI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI XbaI XcmI XhoI
XmaI ZraI