Restriction map for
pF1KSDA0085
(Linear) (Six-base) MAPSORT of: KIAA0085.seq Check: 4293 from: 1 to: 786
KIAA0085.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 608 786
Size: 608 178
AflIII A'CryG_T
Cuts at: 0 611 786
Size: 611 175
AlwNI CAG_nnn'CTG
Cuts at: 0 423 786
Size: 423 363
ApaI G_GGCC'C
Cuts at: 0 760 786
Size: 760 26
BanII G_rGCy'C
Cuts at: 0 337 760 786
Size: 337 423 26
Fragments arranged by size:
423 337 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 748 786
Size: 748 38
BclI T'GATC_A
Cuts at: 0 86 563 644 786
Size: 86 477 81 142
Fragments arranged by size:
477 142 86 81
Bme1580I G_kGCm'C
Cuts at: 0 476 760 786
Size: 476 284 26
Fragments arranged by size:
476 284 26
BmtI G_CTAG'C
Cuts at: 0 389 786
Size: 389 397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 676 786
Size: 177 499 110
Fragments arranged by size:
499 177 110
Bpu10I CC'TnA_GC
Cuts at: 0 232 436 786
Size: 232 204 350
Fragments arranged by size:
350 232 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 786
Size: 346 440
BsaBI GATnn'nnATC
Cuts at: 0 562 565 786
Size: 562 3 221
Fragments arranged by size:
562 221 3
BsaHI Gr'CG_yC
Cuts at: 0 142 786
Size: 142 644
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 786
Size: 175 30 581
Fragments arranged by size:
581 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 381 682 786
Size: 381 301 104
Fragments arranged by size:
381 301 104
BseYI C'CCAG_C
Cuts at: 0 330 520 759 786
Size: 330 190 239 27
Fragments arranged by size:
330 239 190 27
BsiHKAI G_wGCw'C
Cuts at: 0 116 786
Size: 116 670
Bsp1286I G_dGCh'C
Cuts at: 0 116 337 476 760 786
Size: 116 221 139 284 26
Fragments arranged by size:
284 221 139 116 26
BspHI T'CATG_A
Cuts at: 0 89 786
Size: 89 697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 281 786
Size: 40 241 505
Fragments arranged by size:
505 241 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 786
Size: 69 717
Bsu36I CC'TnA_GG
Cuts at: 0 172 786
Size: 172 614
BtgI C'CryG_G
Cuts at: 0 132 786
Size: 132 654
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 561 786
Size: 39 522 225
Fragments arranged by size:
522 225 39
EaeI y'GGCC_r
Cuts at: 0 71 786
Size: 71 715
EarI CTCTTCn'nnn_
Cuts at: 0 223 667 786
Size: 223 444 119
Fragments arranged by size:
444 223 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 13 786
Size: 13 773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 676 786
Size: 177 499 110
Fragments arranged by size:
499 177 110
EcoO109I rG'GnC_Cy
Cuts at: 0 756 786
Size: 756 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 786
Size: 175 32 579
Fragments arranged by size:
579 175 32
MscI TGG'CCA
Cuts at: 0 73 786
Size: 73 713
MslI CAynn'nnrTG
Cuts at: 0 211 469 556 786
Size: 211 258 87 230
Fragments arranged by size:
258 230 211 87
MspA1I CmG'CkG
Cuts at: 0 420 786
Size: 420 366
NcoI C'CATG_G
Cuts at: 0 132 786
Size: 132 654
NheI G'CTAG_C
Cuts at: 0 385 786
Size: 385 401
NspI r_CATG'y
Cuts at: 0 615 786
Size: 615 171
PciI A'CATG_T
Cuts at: 0 611 786
Size: 611 175
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 786
Size: 699 87
PfoI T'CCnGG_A
Cuts at: 0 373 786
Size: 373 413
PspOMI G'GGCC_C
Cuts at: 0 756 786
Size: 756 30
PstI C_TGCA'G
Cuts at: 0 419 457 786
Size: 419 38 329
Fragments arranged by size:
419 329 38
PvuII CAG'CTG
Cuts at: 0 420 786
Size: 420 366
SapI GCTCTTCn'nnn_
Cuts at: 0 223 786
Size: 223 563
SfcI C'TryA_G
Cuts at: 0 415 453 636 786
Size: 415 38 183 150
Fragments arranged by size:
415 183 150 38
SmlI C'TyrA_G
Cuts at: 0 361 786
Size: 361 425
StyI C'CwwG_G
Cuts at: 0 132 786
Size: 132 654
Enzymes that do cut:
AccI AflIII AlwNI ApaI BanII BbsI BclI Bme1580I
BmtI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspHI BspMI BstXI Bsu36I BtgI
BtgZI EaeI EarI EciI Eco57MI EcoO109I Hin4I MscI
MslI MspA1I NcoI NheI NspI PciI PflMI PfoI
PspOMI PstI PvuII SapI SfcI SmlI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI
BplI BsaI BsaAI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI