Restriction map for
pF1KSDA0088
(Linear) (Six-base) MAPSORT of: KIAA0088.seq Check: 247 from: 1 to: 2541
KIAA0088.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AatII G_ACGT'C
Cuts at: 0 800 2541
Size: 800 1741
AccI GT'mk_AC
Cuts at: 0 1109 2541
Size: 1109 1432
Acc65I G'GTAC_C
Cuts at: 0 1697 2541
Size: 1697 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 2150 2366 2541
Size: 532 1618 216 175
Fragments arranged by size:
1618 532 216 175
AfeI AGC'GCT
Cuts at: 0 1072 1477 1537 2541
Size: 1072 405 60 1004
Fragments arranged by size:
1072 1004 405 60
AflIII A'CryG_T
Cuts at: 0 1277 2258 2541
Size: 1277 981 283
Fragments arranged by size:
1277 981 283
AlwNI CAG_nnn'CTG
Cuts at: 0 784 1987 2079 2344 2413 2541
Size: 784 1203 92 265 69 128
Fragments arranged by size:
1203 784 265 128 92 69
ApaI G_GGCC'C
Cuts at: 0 258 306 818 2541
Size: 258 48 512 1723
Fragments arranged by size:
1723 512 258 48
ApaLI G'TGCA_C
Cuts at: 0 1444 2541
Size: 1444 1097
AvaI C'yCGr_G
Cuts at: 0 224 1184 2541
Size: 224 960 1357
Fragments arranged by size:
1357 960 224
AvrII C'CTAG_G
Cuts at: 0 259 2541
Size: 259 2282
BamHI G'GATC_C
Cuts at: 0 482 2541
Size: 482 2059
BanI G'GyrC_C
Cuts at: 0 1697 2541
Size: 1697 844
BanII G_rGCy'C
Cuts at: 0 258 306 742 818 1306 1995 2240 2541
Size: 258 48 436 76 488 689 245 301
Fragments arranged by size:
689 488 436 301 258 245 76 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 792 2156 2541
Size: 709 83 1364 385
Fragments arranged by size:
1364 709 385 83
BbvCI CC'TCA_GC
Cuts at: 0 137 290 1049 2541
Size: 137 153 759 1492
Fragments arranged by size:
1492 759 153 137
BciVI GTATCCnnnnn_n'
Cuts at: 0 2116 2541
Size: 2116 425
BclI T'GATC_A
Cuts at: 0 960 1938 2541
Size: 960 978 603
Fragments arranged by size:
978 960 603
BglI GCCn_nnn'nGGC
Cuts at: 0 1270 2541
Size: 1270 1271
BglII A'GATC_T
Cuts at: 0 1589 2541
Size: 1589 952
BlpI GC'TnA_GC
Cuts at: 0 1224 1305 2334 2541
Size: 1224 81 1029 207
Fragments arranged by size:
1224 1029 207 81
Bme1580I G_kGCm'C
Cuts at: 0 258 306 818 1448 1734 2541
Size: 258 48 512 630 286 807
Fragments arranged by size:
807 630 512 286 258 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 1031 1760 2541
Size: 1031 729 781
Fragments arranged by size:
1031 781 729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 2009 2313 2407 2423 2541
Size: 46 1963 304 94 16 118
Fragments arranged by size:
1963 304 118 94 46 16
Bpu10I CC'TnA_GC
Cuts at: 0 137 290 488 1049 2541
Size: 137 153 198 561 1492
Fragments arranged by size:
1492 561 198 153 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1363 2213 2541
Size: 1087 276 850 328
Fragments arranged by size:
1087 850 328 276
BsaI GGTCTCn'nnnn_
Cuts at: 0 270 661 742 2467 2541
Size: 270 391 81 1725 74
Fragments arranged by size:
1725 391 270 81 74
BsaHI Gr'CG_yC
Cuts at: 0 797 2541
Size: 797 1744
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2020 2050 2541
Size: 2020 30 491
Fragments arranged by size:
2020 491 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 403 1165 2541
Size: 358 45 762 1376
Fragments arranged by size:
1376 762 358 45
BseYI C'CCAG_C
Cuts at: 0 285 305 810 1388 1403 1532 2541
Size: 285 20 505 578 15 129 1009
Fragments arranged by size:
1009 578 505 285 129 20 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1921 2541
Size: 1921 620
BsiHKAI G_wGCw'C
Cuts at: 0 614 1412 1448 2240 2541
Size: 614 798 36 792 301
Fragments arranged by size:
798 792 614 301 36
BsmI GAATG_Cn'
Cuts at: 0 1874 2541
Size: 1874 667
Bsp1286I G_dGCh'C
Cuts at: 0 258 306 614 742 818 1306 1412 1448
Size: 258 48 308 128 76 488 106 36
Cuts at: 1448 1734 1995 2240 2541
Size: 286 261 245 301
Fragments arranged by size:
488 308 301 286 261 258 245 128
106 76 48 36
BspHI T'CATG_A
Cuts at: 0 1883 2541
Size: 1883 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 740 977 2093 2541
Size: 740 237 1116 448
Fragments arranged by size:
1116 740 448 237
BsrBI CCG'CTC
Cuts at: 0 2379 2541
Size: 2379 162
BsrGI T'GTAC_A
Cuts at: 0 575 2541
Size: 575 1966
BssSI C'ACGA_G
Cuts at: 0 1147 2541
Size: 1147 1394
BstEII G'GTnAC_C
Cuts at: 0 1231 1368 2541
Size: 1231 137 1173
Fragments arranged by size:
1231 1173 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 383 438 1396 1540 2541
Size: 383 55 958 144 1001
Fragments arranged by size:
1001 958 383 144 55
BstYI r'GATC_y
Cuts at: 0 482 1589 2434 2541
Size: 482 1107 845 107
Fragments arranged by size:
1107 845 482 107
Bsu36I CC'TnA_GG
Cuts at: 0 311 505 1364 1910 2216 2541
Size: 311 194 859 546 306 325
Fragments arranged by size:
859 546 325 311 306 194
BtgI C'CryG_G
Cuts at: 0 131 1765 1995 2541
Size: 131 1634 230 546
Fragments arranged by size:
1634 546 230 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 311 616 2541
Size: 311 305 1925
Fragments arranged by size:
1925 311 305
BtsI GCAGTG_nn'
Cuts at: 0 692 1565 2541
Size: 692 873 976
Fragments arranged by size:
976 873 692
EaeI y'GGCC_r
Cuts at: 0 169 1100 1216 1508 2019 2541
Size: 169 931 116 292 511 522
Fragments arranged by size:
931 522 511 292 169 116
EarI CTCTTCn'nnn_
Cuts at: 0 886 2541
Size: 886 1655
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 1104 1660 2541
Size: 166 938 556 881
Fragments arranged by size:
938 881 556 166
EcoICRI GAG'CTC
Cuts at: 0 2238 2541
Size: 2238 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 532 2009 2150 2313 2366 2407 2423
Size: 46 486 1477 141 163 53 41 16
Cuts at: 2423 2541
Size: 118
Fragments arranged by size:
1477 486 163 141 118 53 46 41
16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 2021 2541
Size: 190 1831 520
Fragments arranged by size:
1831 520 190
EcoO109I rG'GnC_Cy
Cuts at: 0 142 254 255 302 814 815 1032 1262
Size: 142 112 1 47 512 1 217 230
Cuts at: 1262 1514 1889 2541
Size: 252 375 652
Fragments arranged by size:
652 512 375 252 230 217 142 112
47 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 2541
Size: 174 2367
FspI TGC'GCA
Cuts at: 0 2494 2541
Size: 2494 47
HaeII r_GCGC'y
Cuts at: 0 1074 1479 1539 2541
Size: 1074 405 60 1002
Fragments arranged by size:
1074 1002 405 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2117 2149 2541
Size: 2117 32 392
Fragments arranged by size:
2117 392 32
HincII GTy'rAC
Cuts at: 0 128 2541
Size: 128 2413
HpaI GTT'AAC
Cuts at: 0 128 2541
Size: 128 2413
KpnI G_GTAC'C
Cuts at: 0 1701 2541
Size: 1701 840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 889 2541
Size: 713 176 1652
Fragments arranged by size:
1652 713 176
MscI TGG'CCA
Cuts at: 0 1102 1218 1510 2021 2541
Size: 1102 116 292 511 520
Fragments arranged by size:
1102 520 511 292 116
MslI CAynn'nnrTG
Cuts at: 0 436 476 1007 1123 1415 2509 2541
Size: 436 40 531 116 292 1094 32
Fragments arranged by size:
1094 531 436 292 116 40 32
MspA1I CmG'CkG
Cuts at: 0 289 1228 2413 2541
Size: 289 939 1185 128
Fragments arranged by size:
1185 939 289 128
NcoI C'CATG_G
Cuts at: 0 131 1765 1995 2541
Size: 131 1634 230 546
Fragments arranged by size:
1634 546 230 131
NruI TCG'CGA
Cuts at: 0 635 2541
Size: 635 1906
NspI r_CATG'y
Cuts at: 0 475 496 1006 1281 1738 2541
Size: 475 21 510 275 457 803
Fragments arranged by size:
803 510 475 457 275 21
PasI CC'CwG_GG
Cuts at: 0 323 2541
Size: 323 2218
PciI A'CATG_T
Cuts at: 0 1277 2541
Size: 1277 1264
PpuMI rG'GwC_Cy
Cuts at: 0 142 1032 2541
Size: 142 890 1509
Fragments arranged by size:
1509 890 142
PshAI GACnn'nnGTC
Cuts at: 0 200 1037 2541
Size: 200 837 1504
Fragments arranged by size:
1504 837 200
PspOMI G'GGCC_C
Cuts at: 0 254 302 814 2541
Size: 254 48 512 1727
Fragments arranged by size:
1727 512 254 48
PstI C_TGCA'G
Cuts at: 0 666 737 2541
Size: 666 71 1804
Fragments arranged by size:
1804 666 71
PvuII CAG'CTG
Cuts at: 0 289 1228 2413 2541
Size: 289 939 1185 128
Fragments arranged by size:
1185 939 289 128
SacI G_AGCT'C
Cuts at: 0 2240 2541
Size: 2240 301
SanDI GG'GwC_CC
Cuts at: 0 142 1032 2541
Size: 142 890 1509
Fragments arranged by size:
1509 890 142
SbfI CC_TGCA'GG
Cuts at: 0 737 2541
Size: 737 1804
ScaI AGT'ACT
Cuts at: 0 1945 2541
Size: 1945 596
SfcI C'TryA_G
Cuts at: 0 662 733 2541
Size: 662 71 1808
Fragments arranged by size:
1808 662 71
SmaI CCC'GGG
Cuts at: 0 1186 2541
Size: 1186 1355
SmlI C'TyrA_G
Cuts at: 0 1066 1378 2228 2541
Size: 1066 312 850 313
Fragments arranged by size:
1066 850 313 312
SphI G_CATG'C
Cuts at: 0 496 2541
Size: 496 2045
StyI C'CwwG_G
Cuts at: 0 131 259 1765 1808 1995 2022 2541
Size: 131 128 1506 43 187 27 519
Fragments arranged by size:
1506 519 187 131 128 43 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 212 336 1642 1696 1887 2541
Size: 212 124 1306 54 191 654
Fragments arranged by size:
1306 654 212 191 124 54
TatI w'GTAC_w
Cuts at: 0 575 1943 2541
Size: 575 1368 598
Fragments arranged by size:
1368 598 575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 2541
Size: 158 32 2351
Fragments arranged by size:
2351 158 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1747 2062 2541
Size: 1747 315 479
Fragments arranged by size:
1747 479 315
XmaI C'CCGG_G
Cuts at: 0 1184 2541
Size: 1184 1357
XmnI GAAnn'nnTTC
Cuts at: 0 1874 2541
Size: 1874 667
ZraI GAC'GTC
Cuts at: 0 798 2541
Size: 798 1743
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AlwNI ApaI
ApaLI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HpaI KpnI MmeI MscI MslI
MspA1I NcoI NruI NspI PasI PciI PpuMI PshAI
PspOMI PstI PvuII SacI SanDI SbfI ScaI SfcI
SmaI SmlI SphI StyI TaqII TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AhdI AleI AlfI AloI
ApoI AscI AseI AsiSI BaeI BbeI BcgI BfrBI
BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstBI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspAI HindIII KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NsiI PacI PflMI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI