Restriction map for
pF1KA0089
(Linear) (Six-base) MAPSORT of: KIAA0089.seq Check: 4085 from: 1 to: 1053
KIAA0089.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AccI GT'mk_AC
Cuts at: 0 748 1053
Size: 748 305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 938 1015 1053
Size: 76 862 77 38
Fragments arranged by size:
862 77 76 38
AfeI AGC'GCT
Cuts at: 0 344 1053
Size: 344 709
AflII C'TTAA_G
Cuts at: 0 613 1053
Size: 613 440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 929 1053
Size: 929 124
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 33 65 1053
Size: 33 32 988
Fragments arranged by size:
988 33 32
AlwNI CAG_nnn'CTG
Cuts at: 0 308 592 1053
Size: 308 284 461
Fragments arranged by size:
461 308 284
ApaI G_GGCC'C
Cuts at: 0 382 1053
Size: 382 671
ApoI r'AATT_y
Cuts at: 0 97 558 1053
Size: 97 461 495
Fragments arranged by size:
495 461 97
AvaI C'yCGr_G
Cuts at: 0 36 382 1053
Size: 36 346 671
Fragments arranged by size:
671 346 36
BanI G'GyrC_C
Cuts at: 0 9 1053
Size: 9 1044
BanII G_rGCy'C
Cuts at: 0 37 382 1053
Size: 37 345 671
Fragments arranged by size:
671 345 37
BbeI G_GCGC'C
Cuts at: 0 13 1053
Size: 13 1040
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 1021 1053
Size: 856 165 32
Fragments arranged by size:
856 165 32
BclI T'GATC_A
Cuts at: 0 788 1053
Size: 788 265
BglII A'GATC_T
Cuts at: 0 986 1053
Size: 986 67
Bme1580I G_kGCm'C
Cuts at: 0 335 382 1053
Size: 335 47 671
Fragments arranged by size:
671 335 47
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 332 854 1053
Size: 54 278 522 199
Fragments arranged by size:
522 278 199 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 1053
Size: 487 566
BsaHI Gr'CG_yC
Cuts at: 0 10 1053
Size: 10 1043
BseYI C'CCAG_C
Cuts at: 0 345 637 1053
Size: 345 292 416
Fragments arranged by size:
416 345 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1053
Size: 274 779
BsiEI CG_ry'CG
Cuts at: 0 683 1053
Size: 683 370
Bsp1286I G_dGCh'C
Cuts at: 0 37 335 382 1053
Size: 37 298 47 671
Fragments arranged by size:
671 298 47 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 1053
Size: 275 778
BsrDI GCAATG_nn'
Cuts at: 0 458 1053
Size: 458 595
BstBI TT'CG_AA
Cuts at: 0 564 1053
Size: 564 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1053
Size: 471 582
BstYI r'GATC_y
Cuts at: 0 725 986 1053
Size: 725 261 67
Fragments arranged by size:
725 261 67
BtsI GCAGTG_nn'
Cuts at: 0 983 1053
Size: 983 70
EaeI y'GGCC_r
Cuts at: 0 680 779 824 1053
Size: 680 99 45 229
Fragments arranged by size:
680 229 99 45
EagI C'GGCC_G
Cuts at: 0 680 1053
Size: 680 373
EarI CTCTTCn'nnn_
Cuts at: 0 538 854 1053
Size: 538 316 199
Fragments arranged by size:
538 316 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 679 825 1053
Size: 679 146 228
Fragments arranged by size:
679 228 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 938 1015 1053
Size: 76 862 77 38
Fragments arranged by size:
862 77 76 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 1053
Size: 361 692
EcoO109I rG'GnC_Cy
Cuts at: 0 378 379 1053
Size: 378 1 674
Fragments arranged by size:
674 378 1
HaeII r_GCGC'y
Cuts at: 0 13 346 1053
Size: 13 333 707
Fragments arranged by size:
707 333 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1053
Size: 1006 32 15
Fragments arranged by size:
1006 32 15
KasI G'GCGC_C
Cuts at: 0 9 1053
Size: 9 1044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1053
Size: 845 208
MslI CAynn'nnrTG
Cuts at: 0 182 439 706 1053
Size: 182 257 267 347
Fragments arranged by size:
347 267 257 182
MspA1I CmG'CkG
Cuts at: 0 7 55 659 1053
Size: 7 48 604 394
Fragments arranged by size:
604 394 48 7
NarI GG'CG_CC
Cuts at: 0 10 1053
Size: 10 1043
PfoI T'CCnGG_A
Cuts at: 0 198 1053
Size: 198 855
PspOMI G'GGCC_C
Cuts at: 0 378 1053
Size: 378 675
PstI C_TGCA'G
Cuts at: 0 480 551 978 1053
Size: 480 71 427 75
Fragments arranged by size:
480 427 75 71
PvuII CAG'CTG
Cuts at: 0 55 1053
Size: 55 998
SfcI C'TryA_G
Cuts at: 0 476 547 749 974 1053
Size: 476 71 202 225 79
Fragments arranged by size:
476 225 202 79 71
SfoI GGC'GCC
Cuts at: 0 11 1053
Size: 11 1042
SmlI C'TyrA_G
Cuts at: 0 613 1053
Size: 613 440
StuI AGG'CCT
Cuts at: 0 832 1053
Size: 832 221
StyI C'CwwG_G
Cuts at: 0 897 1053
Size: 897 156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 861 1053
Size: 342 519 192
Fragments arranged by size:
519 342 192
Enzymes that do cut:
AccI AcuI AfeI AflII AlfI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BclI BglII
Bme1580I BmrI BsaI BsaHI BseYI BsgI BsiEI Bsp1286I
BspMI BsrDI BstBI BstXI BstYI BtsI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MmeI MslI MspA1I NarI PfoI PspOMI PstI PvuII
SfcI SfoI SmlI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI