Restriction map for
pF1KSDA0098
(Linear) (Six-base) MAPSORT of: KIAA0098.seq Check: 8930 from: 1 to: 1623
KIAA0098.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 928 1623
Size: 814 114 695
Fragments arranged by size:
814 695 114
AflII C'TTAA_G
Cuts at: 0 231 1623
Size: 231 1392
AflIII A'CryG_T
Cuts at: 0 754 1623
Size: 754 869
AhdI GACnn_n'nnGTC
Cuts at: 0 72 1623
Size: 72 1551
AleI CACnn'nnGTG
Cuts at: 0 322 1623
Size: 322 1301
ApoI r'AATT_y
Cuts at: 0 850 1623
Size: 850 773
BanI G'GyrC_C
Cuts at: 0 38 340 1623
Size: 38 302 1283
Fragments arranged by size:
1283 302 38
BanII G_rGCy'C
Cuts at: 0 523 1043 1623
Size: 523 520 580
Fragments arranged by size:
580 523 520
BbeI G_GCGC'C
Cuts at: 0 42 1623
Size: 42 1581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 437 1623
Size: 403 34 1186
Fragments arranged by size:
1186 403 34
BclI T'GATC_A
Cuts at: 0 249 1178 1623
Size: 249 929 445
Fragments arranged by size:
929 445 249
BglII A'GATC_T
Cuts at: 0 1082 1623
Size: 1082 541
Bme1580I G_kGCm'C
Cuts at: 0 1290 1321 1623
Size: 1290 31 302
Fragments arranged by size:
1290 302 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1122 1384 1623
Size: 676 446 262 239
Fragments arranged by size:
676 446 262 239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 112 1623
Size: 85 27 1511
Fragments arranged by size:
1511 85 27
BsaBI GATnn'nnATC
Cuts at: 0 248 1000 1623
Size: 248 752 623
Fragments arranged by size:
752 623 248
BsaHI Gr'CG_yC
Cuts at: 0 4 39 1623
Size: 4 35 1584
Fragments arranged by size:
1584 35 4
BsaWI w'CCGG_w
Cuts at: 0 1226 1623
Size: 1226 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 1623
Size: 314 30 1279
Fragments arranged by size:
1279 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1178 1201 1277 1623
Size: 1178 23 76 346
Fragments arranged by size:
1178 346 76 23
BseYI C'CCAG_C
Cuts at: 0 516 968 1056 1623
Size: 516 452 88 567
Fragments arranged by size:
567 516 452 88
BsiHKAI G_wGCw'C
Cuts at: 0 1043 1623
Size: 1043 580
BsmI GAATG_Cn'
Cuts at: 0 380 576 1623
Size: 380 196 1047
Fragments arranged by size:
1047 380 196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 599 1623
Size: 599 1024
Bsp1286I G_dGCh'C
Cuts at: 0 523 1043 1290 1321 1623
Size: 523 520 247 31 302
Fragments arranged by size:
523 520 302 247 31
BspEI T'CCGG_A
Cuts at: 0 1226 1623
Size: 1226 397
BsrBI CCG'CTC
Cuts at: 0 600 1623
Size: 600 1023
BsrGI T'GTAC_A
Cuts at: 0 1074 1623
Size: 1074 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 324 1623
Size: 270 54 1299
Fragments arranged by size:
1299 270 54
BstYI r'GATC_y
Cuts at: 0 1082 1623
Size: 1082 541
BtgI C'CryG_G
Cuts at: 0 8 1376 1623
Size: 8 1368 247
Fragments arranged by size:
1368 247 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 987 1623
Size: 987 636
BtsI GCAGTG_nn'
Cuts at: 0 1132 1623
Size: 1132 491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 707 742 1623
Size: 707 35 881
Fragments arranged by size:
881 707 35
EarI CTCTTCn'nnn_
Cuts at: 0 851 1623
Size: 851 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1032 1324 1623
Size: 1032 292 299
Fragments arranged by size:
1032 299 292
EcoICRI GAG'CTC
Cuts at: 0 1041 1623
Size: 1041 582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 814 928 1122 1384 1623
Size: 676 138 114 194 262 239
Fragments arranged by size:
676 262 239 194 138 114
EcoO109I rG'GnC_Cy
Cuts at: 0 14 96 979 1623
Size: 14 82 883 644
Fragments arranged by size:
883 644 82 14
EcoRV GAT'ATC
Cuts at: 0 1280 1550 1623
Size: 1280 270 73
Fragments arranged by size:
1280 270 73
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1077 1623
Size: 1077 546
HaeII r_GCGC'y
Cuts at: 0 42 1623
Size: 42 1581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 314 346 790 822 1004 1036
Size: 58 32 224 32 444 32 182 32
Cuts at: 1036 1623
Size: 587
Fragments arranged by size:
587 444 224 182 58 32 32 32
32
HincII GTy'rAC
Cuts at: 0 471 534 612 1623
Size: 471 63 78 1011
Fragments arranged by size:
1011 471 78 63
KasI G'GCGC_C
Cuts at: 0 38 1623
Size: 38 1585
MfeI C'AATT_G
Cuts at: 0 364 1623
Size: 364 1259
MslI CAynn'nnrTG
Cuts at: 0 322 1375 1623
Size: 322 1053 248
Fragments arranged by size:
1053 322 248
NarI GG'CG_CC
Cuts at: 0 39 1623
Size: 39 1584
NcoI C'CATG_G
Cuts at: 0 8 1376 1623
Size: 8 1368 247
Fragments arranged by size:
1368 247 8
NspI r_CATG'y
Cuts at: 0 758 1516 1623
Size: 758 758 107
Fragments arranged by size:
758 758 107
PciI A'CATG_T
Cuts at: 0 754 1623
Size: 754 869
PpuMI rG'GwC_Cy
Cuts at: 0 14 979 1623
Size: 14 965 644
Fragments arranged by size:
965 644 14
PshAI GACnn'nnGTC
Cuts at: 0 808 1426 1623
Size: 808 618 197
Fragments arranged by size:
808 618 197
PsiI TTA'TAA
Cuts at: 0 821 1623
Size: 821 802
SacI G_AGCT'C
Cuts at: 0 1043 1623
Size: 1043 580
SanDI GG'GwC_CC
Cuts at: 0 14 1623
Size: 14 1609
SfcI C'TryA_G
Cuts at: 0 128 584 1623
Size: 128 456 1039
Fragments arranged by size:
1039 456 128
SfoI GGC'GCC
Cuts at: 0 40 1623
Size: 40 1583
SmlI C'TyrA_G
Cuts at: 0 91 100 231 1623
Size: 91 9 131 1392
Fragments arranged by size:
1392 131 91 9
StyI C'CwwG_G
Cuts at: 0 8 1376 1623
Size: 8 1368 247
Fragments arranged by size:
1368 247 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 389 401 722 786 1438 1623
Size: 389 12 321 64 652 185
Fragments arranged by size:
652 389 321 185 64 12
TatI w'GTAC_w
Cuts at: 0 1074 1623
Size: 1074 549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 1623
Size: 270 1353
Enzymes that do cut:
AcuI AflII AflIII AhdI AleI ApoI BanI BanII
BbeI BcgI BclI BglII Bme1580I BpmI BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrGI BstXI BstYI BtgI BtgZI
BtsI CspCI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HincII KasI MfeI MslI NarI
NcoI NspI PciI PpuMI PshAI PsiI SacI SanDI
SfcI SfoI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsgI BsiEI BsiWI BspHI BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraI
DraIII DrdI EaeI EagI EcoNI EcoRI FseI FspI
FspAI HindIII HpaI KpnI MluI MmeI MscI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI