Restriction map for
pF1KA0099
(Linear) (Six-base) MAPSORT of: KIAA0099.seq Check: 9660 from: 1 to: 3486
KIAA0099.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AatII G_ACGT'C
Cuts at: 0 875 3486
Size: 875 2611
AccI GT'mk_AC
Cuts at: 0 1406 3486
Size: 1406 2080
Acc65I G'GTAC_C
Cuts at: 0 1098 2441 3486
Size: 1098 1343 1045
Fragments arranged by size:
1343 1098 1045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 2315 2319 2744 3486
Size: 1057 1258 4 425 742
Fragments arranged by size:
1258 1057 742 425 4
AfeI AGC'GCT
Cuts at: 0 1299 3486
Size: 1299 2187
AflIII A'CryG_T
Cuts at: 0 1053 3069 3486
Size: 1053 2016 417
Fragments arranged by size:
2016 1053 417
AgeI A'CCGG_T
Cuts at: 0 2438 3486
Size: 2438 1048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1235 1451 3173 3486
Size: 1235 216 1722 313
Fragments arranged by size:
1722 1235 313 216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1721 1753 3486
Size: 1721 32 1733
Fragments arranged by size:
1733 1721 32
AlwNI CAG_nnn'CTG
Cuts at: 0 527 1123 2293 2664 3486
Size: 527 596 1170 371 822
Fragments arranged by size:
1170 822 596 527 371
ApaI G_GGCC'C
Cuts at: 0 3458 3486
Size: 3458 28
ApaLI G'TGCA_C
Cuts at: 0 3231 3486
Size: 3231 255
ApoI r'AATT_y
Cuts at: 0 1602 2485 2641 3154 3486
Size: 1602 883 156 513 332
Fragments arranged by size:
1602 883 513 332 156
AseI AT'TA_AT
Cuts at: 0 2777 3486
Size: 2777 709
AvaI C'yCGr_G
Cuts at: 0 687 2340 3486
Size: 687 1653 1146
Fragments arranged by size:
1653 1146 687
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1082 1115 3486
Size: 1082 33 2371
Fragments arranged by size:
2371 1082 33
BamHI G'GATC_C
Cuts at: 0 1575 1978 2221 3486
Size: 1575 403 243 1265
Fragments arranged by size:
1575 1265 403 243
BanI G'GyrC_C
Cuts at: 0 988 1098 1690 1966 2441 3486
Size: 988 110 592 276 475 1045
Fragments arranged by size:
1045 988 592 475 276 110
BanII G_rGCy'C
Cuts at: 0 682 1734 1915 3458 3486
Size: 682 1052 181 1543 28
Fragments arranged by size:
1543 1052 682 181 28
BbeI G_GCGC'C
Cuts at: 0 1970 3486
Size: 1970 1516
BbsI GAAGACnn'nnnn_
Cuts at: 0 582 2556 3486
Size: 582 1974 930
Fragments arranged by size:
1974 930 582
BbvCI CC'TCA_GC
Cuts at: 0 1776 1805 3486
Size: 1776 29 1681
Fragments arranged by size:
1776 1681 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1944 1978 3486
Size: 1944 34 1508
Fragments arranged by size:
1944 1508 34
BclI T'GATC_A
Cuts at: 0 507 3486
Size: 507 2979
BfrBI ATG'CAT
Cuts at: 0 2858 3351 3486
Size: 2858 493 135
Fragments arranged by size:
2858 493 135
BlpI GC'TnA_GC
Cuts at: 0 963 1205 1490 2549 3486
Size: 963 242 285 1059 937
Fragments arranged by size:
1059 963 937 285 242
Bme1580I G_kGCm'C
Cuts at: 0 1695 3235 3458 3486
Size: 1695 1540 223 28
Fragments arranged by size:
1695 1540 223 28
BmgBI CAC'GTC
Cuts at: 0 2694 3318 3486
Size: 2694 624 168
Fragments arranged by size:
2694 624 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 1753 2871 3486
Size: 344 1409 1118 615
Fragments arranged by size:
1409 1118 615 344
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 1026 2366 3486
Size: 451 575 1340 1120
Fragments arranged by size:
1340 1120 575 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 430 2270 2310 2551 2563 2989 3097
Size: 168 262 1840 40 241 12 426 108
Cuts at: 3097 3439 3486
Size: 342 47
Fragments arranged by size:
1840 426 342 262 241 168 108 47
40 12
Bpu10I CC'TnA_GC
Cuts at: 0 1776 1805 2125 3486
Size: 1776 29 320 1361
Fragments arranged by size:
1776 1361 320 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 2767 3486
Size: 62 2705 719
Fragments arranged by size:
2705 719 62
BsaAI yAC'GTr
Cuts at: 0 2844 3199 3297 3486
Size: 2844 355 98 189
Fragments arranged by size:
2844 355 189 98
BsaHI Gr'CG_yC
Cuts at: 0 872 1967 3486
Size: 872 1095 1519
Fragments arranged by size:
1519 1095 872
BsaWI w'CCGG_w
Cuts at: 0 1653 2438 3486
Size: 1653 785 1048
Fragments arranged by size:
1653 1048 785
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 658 688 1721 1751 2184 2214 3486
Size: 658 30 1033 30 433 30 1272
Fragments arranged by size:
1272 1033 658 433 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 299 311 826 1535 2127 3022 3486
Size: 299 12 515 709 592 895 464
Fragments arranged by size:
895 709 592 515 464 299 12
BseYI C'CCAG_C
Cuts at: 0 172 554 968 1359 1811 1817 1919 3486
Size: 172 382 414 391 452 6 102 1567
Fragments arranged by size:
1567 452 414 391 382 172 102 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 871 1766 3486
Size: 589 282 895 1720
Fragments arranged by size:
1720 895 589 282
BsiEI CG_ry'CG
Cuts at: 0 634 3087 3486
Size: 634 2453 399
Fragments arranged by size:
2453 634 399
BsiHKAI G_wGCw'C
Cuts at: 0 682 1734 2975 3083 3218 3235 3486
Size: 682 1052 1241 108 135 17 251
Fragments arranged by size:
1241 1052 682 251 135 108 17
BsiWI C'GTAC_G
Cuts at: 0 3199 3486
Size: 3199 287
BsmI GAATG_Cn'
Cuts at: 0 1710 3486
Size: 1710 1776
Bsp1286I G_dGCh'C
Cuts at: 0 682 1695 1734 1915 2975 3083 3218 3235
Size: 682 1013 39 181 1060 108 135 17
Cuts at: 3235 3458 3486
Size: 223 28
Fragments arranged by size:
1060 1013 682 223 181 135 108 39
28 17
BspHI T'CATG_A
Cuts at: 0 387 3486
Size: 387 3099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1086 1243 1497 2760 3486
Size: 1086 157 254 1263 726
Fragments arranged by size:
1263 1086 726 254 157
BsrDI GCAATG_nn'
Cuts at: 0 3135 3171 3486
Size: 3135 36 315
Fragments arranged by size:
3135 315 36
BsrFI r'CCGG_y
Cuts at: 0 2438 3486
Size: 2438 1048
BsrGI T'GTAC_A
Cuts at: 0 2868 3036 3486
Size: 2868 168 450
Fragments arranged by size:
2868 450 168
BssHII G'CGCG_C
Cuts at: 0 3207 3486
Size: 3207 279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 3486
Size: 438 3048
BstBI TT'CG_AA
Cuts at: 0 2430 3486
Size: 2430 1056
BstXI CCAn_nnnn'nTGG
Cuts at: 0 999 1384 3297 3486
Size: 999 385 1913 189
Fragments arranged by size:
1913 999 385 189
BstYI r'GATC_y
Cuts at: 0 199 927 1575 1978 2221 3356 3486
Size: 199 728 648 403 243 1135 130
Fragments arranged by size:
1135 728 648 403 243 199 130
Bsu36I CC'TnA_GG
Cuts at: 0 3092 3486
Size: 3092 394
BtgI C'CryG_G
Cuts at: 0 371 1046 1515 2150 3486
Size: 371 675 469 635 1336
Fragments arranged by size:
1336 675 635 469 371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 436 1233 3354 3486
Size: 436 797 2121 132
Fragments arranged by size:
2121 797 436 132
BtsI GCAGTG_nn'
Cuts at: 0 38 1604 2034 2067 2268 2316 3486
Size: 38 1566 430 33 201 48 1170
Fragments arranged by size:
1566 1170 430 201 48 38 33
ClaI AT'CG_AT
Cuts at: 0 324 2897 3221 3486
Size: 324 2573 324 265
Fragments arranged by size:
2573 324 324 265
EaeI y'GGCC_r
Cuts at: 0 2802 3410 3486
Size: 2802 608 76
Fragments arranged by size:
2802 608 76
EarI CTCTTCn'nnn_
Cuts at: 0 16 411 1076 1906 2217 2232 2338 2364
Size: 16 395 665 830 311 15 106 26
Cuts at: 2364 3383 3486
Size: 1019 103
Fragments arranged by size:
1019 830 665 395 311 106 103 26
16 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1971 3337 3486
Size: 1971 1366 149
Fragments arranged by size:
1971 1366 149
EcoICRI GAG'CTC
Cuts at: 0 680 1732 3486
Size: 680 1052 1754
Fragments arranged by size:
1754 1052 680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 430 1057 2270 2310 2315 2319 2551
Size: 168 262 627 1213 40 5 4 232
Cuts at: 2551 2563 2744 2989 3097 3439 3486
Size: 12 181 245 108 342 47
Fragments arranged by size:
1213 627 342 262 245 232 181 168
108 47 40 12 5 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 3486
Size: 194 3292
EcoO109I rG'GnC_Cy
Cuts at: 0 3454 3486
Size: 3454 32
EcoRI G'AATT_C
Cuts at: 0 1602 3486
Size: 1602 1884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 52 946 3486
Size: 52 894 2540
Fragments arranged by size:
2540 894 52
HaeII r_GCGC'y
Cuts at: 0 1301 1970 2294 2324 2556 3486
Size: 1301 669 324 30 232 930
Fragments arranged by size:
1301 930 669 324 232 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 1707 1739 2184 2216 2678 2710
Size: 1188 32 487 32 445 32 462 32
Cuts at: 2710 3486
Size: 776
Fragments arranged by size:
1188 776 487 462 445 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1196 3447 3486
Size: 1196 2251 39
Fragments arranged by size:
2251 1196 39
HindIII A'AGCT_T
Cuts at: 0 2232 3486
Size: 2232 1254
KasI G'GCGC_C
Cuts at: 0 1966 3486
Size: 1966 1520
KpnI G_GTAC'C
Cuts at: 0 1102 2445 3486
Size: 1102 1343 1041
Fragments arranged by size:
1343 1102 1041
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 2042 3090 3486
Size: 1037 1005 1048 396
Fragments arranged by size:
1048 1037 1005 396
MscI TGG'CCA
Cuts at: 0 2804 3412 3486
Size: 2804 608 74
Fragments arranged by size:
2804 608 74
MslI CAynn'nnrTG
Cuts at: 0 2939 3271 3486
Size: 2939 332 215
Fragments arranged by size:
2939 332 215
MspA1I CmG'CkG
Cuts at: 0 151 172 972 1158 1201 1231 1267 1330
Size: 151 21 800 186 43 30 36 63
Cuts at: 1330 1363 1441 1447 1591 1780 2523 2548 3336
Size: 33 78 6 144 189 743 25 788
Cuts at: 3336 3486
Size: 150
Fragments arranged by size:
800 788 743 189 186 151 150 144
78 63 43 36 33 30 25 21
6
NarI GG'CG_CC
Cuts at: 0 1967 3486
Size: 1967 1519
NcoI C'CATG_G
Cuts at: 0 371 1046 3486
Size: 371 675 2440
Fragments arranged by size:
2440 675 371
NsiI A_TGCA'T
Cuts at: 0 2860 3353 3486
Size: 2860 493 133
Fragments arranged by size:
2860 493 133
NspI r_CATG'y
Cuts at: 0 14 102 1057 1644 2160 3073 3486
Size: 14 88 955 587 516 913 413
Fragments arranged by size:
955 913 587 516 413 88 14
PasI CC'CwG_GG
Cuts at: 0 1309 1397 1908 1974 3486
Size: 1309 88 511 66 1512
Fragments arranged by size:
1512 1309 511 88 66
PciI A'CATG_T
Cuts at: 0 1053 3069 3486
Size: 1053 2016 417
Fragments arranged by size:
2016 1053 417
PfoI T'CCnGG_A
Cuts at: 0 2142 2966 3486
Size: 2142 824 520
Fragments arranged by size:
2142 824 520
PmlI CAC'GTG
Cuts at: 0 2844 3486
Size: 2844 642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1721 1753 3486
Size: 1721 32 1733
Fragments arranged by size:
1733 1721 32
PsiI TTA'TAA
Cuts at: 0 2072 2117 3486
Size: 2072 45 1369
Fragments arranged by size:
2072 1369 45
PspOMI G'GGCC_C
Cuts at: 0 3454 3486
Size: 3454 32
PstI C_TGCA'G
Cuts at: 0 1344 1452 1596 3486
Size: 1344 108 144 1890
Fragments arranged by size:
1890 1344 144 108
PvuI CG_AT'CG
Cuts at: 0 634 3486
Size: 634 2852
PvuII CAG'CTG
Cuts at: 0 151 172 972 1158 1201 1231 1330 1363
Size: 151 21 800 186 43 30 99 33
Cuts at: 1363 1591 1780 2523 2548 3486
Size: 228 189 743 25 938
Fragments arranged by size:
938 800 743 228 189 186 151 99
43 33 30 25 21
SacI G_AGCT'C
Cuts at: 0 682 1734 3486
Size: 682 1052 1752
Fragments arranged by size:
1752 1052 682
SapI GCTCTTCn'nnn_
Cuts at: 0 411 3486
Size: 411 3075
ScaI AGT'ACT
Cuts at: 0 3427 3486
Size: 3427 59
SexAI A'CCwGG_T
Cuts at: 0 579 3486
Size: 579 2907
SfcI C'TryA_G
Cuts at: 0 203 1340 1448 1592 2710 3486
Size: 203 1137 108 144 1118 776
Fragments arranged by size:
1137 1118 776 203 144 108
SfoI GGC'GCC
Cuts at: 0 1968 3486
Size: 1968 1518
SmaI CCC'GGG
Cuts at: 0 689 3486
Size: 689 2797
SmlI C'TyrA_G
Cuts at: 0 77 2746 3486
Size: 77 2669 740
Fragments arranged by size:
2669 740 77
SphI G_CATG'C
Cuts at: 0 1644 2160 3486
Size: 1644 516 1326
Fragments arranged by size:
1644 1326 516
SspI AAT'ATT
Cuts at: 0 497 3486
Size: 497 2989
StyI C'CwwG_G
Cuts at: 0 371 724 1037 1046 1937 1986 3486
Size: 371 353 313 9 891 49 1500
Fragments arranged by size:
1500 891 371 353 313 49 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 3486
Size: 493 2993
TatI w'GTAC_w
Cuts at: 0 618 2868 3036 3072 3132 3386 3425 3486
Size: 618 2250 168 36 60 254 39 61
Fragments arranged by size:
2250 618 254 168 61 60 39 36
Tth111I GACn'n_nGTC
Cuts at: 0 581 1625 3486
Size: 581 1044 1861
Fragments arranged by size:
1861 1044 581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 1836 1935 2500 3486
Size: 1053 783 99 565 986
Fragments arranged by size:
1053 986 783 565 99
XmaI C'CCGG_G
Cuts at: 0 687 3486
Size: 687 2799
XmnI GAAnn'nnTTC
Cuts at: 0 2426 2681 3486
Size: 2426 255 805
Fragments arranged by size:
2426 805 255
ZraI GAC'GTC
Cuts at: 0 873 3486
Size: 873 2613
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PciI PfoI PmlI PpiI
PsiI PspOMI PstI PvuI PvuII SacI SapI ScaI
SexAI SfcI SfoI SmaI SmlI SphI SspI StyI
TaqII TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AhdI AleI AscI AsiSI AvrII
BciVI BglI BglII BmtI BsaI BsaBI BsmBI BspEI
BsrBI BssSI BstEII BstZ17I CspCI DraI DraIII DrdI
EagI EcoRV FseI FspI FspAI HpaI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PflMI
PmeI PpuMI PshAI PspXI PsrI RsrII SacII SalI
SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI TstI XbaI XhoI