Restriction map for
pF1KA0100
(Linear) (Six-base) MAPSORT of: KIAA0100.seq Check: 100 from: 1 to: 6705
KIAA0100.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 592 1071 1178 3617 4250 6705
Size: 592 479 107 2439 633 2455
Fragments arranged by size:
2455 2439 633 592 479 107
AatII G_ACGT'C
Cuts at: 0 1825 6705
Size: 1825 4880
AccI GT'mk_AC
Cuts at: 0 3129 6705
Size: 3129 3576
Acc65I G'GTAC_C
Cuts at: 0 3092 6705
Size: 3092 3613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 928 1573 1760 2108 2563 4214 4483
Size: 348 580 645 187 348 455 1651 269
Cuts at: 4483 4545 5213 5557 5952 6568 6705
Size: 62 668 344 395 616 137
Fragments arranged by size:
1651 668 645 616 580 455 395 348
348 344 269 187 137 62
AfeI AGC'GCT
Cuts at: 0 336 1856 6705
Size: 336 1520 4849
Fragments arranged by size:
4849 1520 336
AflII C'TTAA_G
Cuts at: 0 169 6705
Size: 169 6536
AflIII A'CryG_T
Cuts at: 0 2024 4601 6705
Size: 2024 2577 2104
Fragments arranged by size:
2577 2104 2024
AleI CACnn'nnGTG
Cuts at: 0 859 3283 3674 6705
Size: 859 2424 391 3031
Fragments arranged by size:
3031 2424 859 391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 668 1018 4123 4919 5458 6203 6705
Size: 668 350 3105 796 539 745 502
Fragments arranged by size:
3105 796 745 668 539 502 350
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5615 5647 6705
Size: 5615 32 1058
Fragments arranged by size:
5615 1058 32
AlwNI CAG_nnn'CTG
Cuts at: 0 822 924 1530 2730 3645 5010 6565 6705
Size: 822 102 606 1200 915 1365 1555 140
Fragments arranged by size:
1555 1365 1200 915 822 606 140 102
ApaI G_GGCC'C
Cuts at: 0 733 3295 3693 6217 6705
Size: 733 2562 398 2524 488
Fragments arranged by size:
2562 2524 733 488 398
ApoI r'AATT_y
Cuts at: 0 1150 3241 3821 5282 6705
Size: 1150 2091 580 1461 1423
Fragments arranged by size:
2091 1461 1423 1150 580
AvaI C'yCGr_G
Cuts at: 0 4067 6705
Size: 4067 2638
BamHI G'GATC_C
Cuts at: 0 152 3303 3465 4320 6705
Size: 152 3151 162 855 2385
Fragments arranged by size:
3151 2385 855 162 152
BanI G'GyrC_C
Cuts at: 0 890 1142 1446 3092 3139 3711 4112 5753
Size: 890 252 304 1646 47 572 401 1641
Cuts at: 5753 6030 6705
Size: 277 675
Fragments arranged by size:
1646 1641 890 675 572 401 304 277
252 47
BanII G_rGCy'C
Cuts at: 0 733 2014 2801 3295 3693 4304 5932 6217
Size: 733 1281 787 494 398 611 1628 285
Cuts at: 6217 6705
Size: 488
Fragments arranged by size:
1628 1281 787 733 611 494 488 398
285
BbeI G_GCGC'C
Cuts at: 0 1450 4116 5757 6705
Size: 1450 2666 1641 948
Fragments arranged by size:
2666 1641 1450 948
BbsI GAAGACnn'nnnn_
Cuts at: 0 1742 3391 4053 6705
Size: 1742 1649 662 2652
Fragments arranged by size:
2652 1742 1649 662
BbvCI CC'TCA_GC
Cuts at: 0 2234 3354 4292 4530 5075 6705
Size: 2234 1120 938 238 545 1630
Fragments arranged by size:
2234 1630 1120 938 545 238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4264 4298 6705
Size: 4264 34 2407
Fragments arranged by size:
4264 2407 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 438 5244 6705
Size: 438 4806 1461
Fragments arranged by size:
4806 1461 438
BclI T'GATC_A
Cuts at: 0 3666 4206 6705
Size: 3666 540 2499
Fragments arranged by size:
3666 2499 540
BfrBI ATG'CAT
Cuts at: 0 1467 6705
Size: 1467 5238
BglII A'GATC_T
Cuts at: 0 383 3015 3179 5220 5405 6705
Size: 383 2632 164 2041 185 1300
Fragments arranged by size:
2632 2041 1300 383 185 164
BlpI GC'TnA_GC
Cuts at: 0 46 1924 2901 4246 6705
Size: 46 1878 977 1345 2459
Fragments arranged by size:
2459 1878 1345 977 46
Bme1580I G_kGCm'C
Cuts at: 0 733 1617 2157 2886 3142 3295 3693 3716
Size: 733 884 540 729 256 153 398 23
Cuts at: 3716 6217 6705
Size: 2501 488
Fragments arranged by size:
2501 884 733 729 540 488 398 256
153 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 1542 1912 3504 6220 6420 6705
Size: 1542 370 1592 2716 200 285
Fragments arranged by size:
2716 1592 1542 370 285 200
BmtI G_CTAG'C
Cuts at: 0 1177 5956 6705
Size: 1177 4779 749
Fragments arranged by size:
4779 1177 749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2646 4320 6705
Size: 2646 1674 2385
Fragments arranged by size:
2646 2385 1674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 799 1993 2218 5512 5878 6463 6705
Size: 595 204 1194 225 3294 366 585 242
Fragments arranged by size:
3294 1194 595 585 366 242 225 204
Bpu10I CC'TnA_GC
Cuts at: 0 232 2159 2234 2381 2951 3354 4292 4530
Size: 232 1927 75 147 570 403 938 238
Cuts at: 4530 5075 5354 5741 6541 6705
Size: 545 279 387 800 164
Fragments arranged by size:
1927 938 800 570 545 403 387 279
238 232 164 147 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1051 3028 4076 5221 5422 6705
Size: 421 630 1977 1048 1145 201 1283
Fragments arranged by size:
1977 1283 1145 1048 630 421 201
BsaI GGTCTCn'nnnn_
Cuts at: 0 2060 6705
Size: 2060 4645
BsaBI GATnn'nnATC
Cuts at: 0 941 4319 6705
Size: 941 3378 2386
Fragments arranged by size:
3378 2386 941
BsaHI Gr'CG_yC
Cuts at: 0 1388 1447 1822 3174 4113 5754 6705
Size: 1388 59 375 1352 939 1641 951
Fragments arranged by size:
1641 1388 1352 951 939 375 59
BsaWI w'CCGG_w
Cuts at: 0 80 2405 2435 6705
Size: 80 2325 30 4270
Fragments arranged by size:
4270 2325 80 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1966 1996 6705
Size: 1966 30 4709
Fragments arranged by size:
4709 1966 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2443 2809 4309 4312 4987 5437 5878 6631
Size: 2443 366 1500 3 675 450 441 753
Cuts at: 6631 6637 6640 6705
Size: 6 3 65
Fragments arranged by size:
2443 1500 753 675 450 441 366 65
6 3 3
BseYI C'CCAG_C
Cuts at: 0 337 3299 3364 3812 3928 3986 4019 4555
Size: 337 2962 65 448 116 58 33 536
Cuts at: 4555 5090 5295 5778 6705
Size: 535 205 483 927
Fragments arranged by size:
2962 927 536 535 483 448 337 205
116 65 58 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1150 1835 2009 2389 3430 4065 4330
Size: 1099 51 685 174 380 1041 635 265
Cuts at: 4330 5023 6705
Size: 693 1682
Fragments arranged by size:
1682 1099 1041 693 685 635 380 265
174 51
BsiHKAI G_wGCw'C
Cuts at: 0 997 1925 2209 2627 2801 4304 4979 5932
Size: 997 928 284 418 174 1503 675 953
Cuts at: 5932 6290 6705
Size: 358 415
Fragments arranged by size:
1503 997 953 928 675 418 415 358
284 174
BsmI GAATG_Cn'
Cuts at: 0 3044 4206 6705
Size: 3044 1162 2499
Fragments arranged by size:
3044 2499 1162
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1813 2860 6705
Size: 1813 1047 3845
Fragments arranged by size:
3845 1813 1047
Bsp1286I G_dGCh'C
Cuts at: 0 733 997 1617 1925 2014 2157 2209 2627
Size: 733 264 620 308 89 143 52 418
Cuts at: 2627 2801 2886 3142 3295 3693 3716 4304 4979
Size: 174 85 256 153 398 23 588 675
Cuts at: 4979 5932 6217 6290 6705
Size: 953 285 73 415
Fragments arranged by size:
953 733 675 620 588 418 415 398
308 285 264 256 174 153 143 89
85 73 52 23
BspEI T'CCGG_A
Cuts at: 0 2435 6705
Size: 2435 4270
BspHI T'CATG_A
Cuts at: 0 2670 3729 5513 6705
Size: 2670 1059 1784 1192
Fragments arranged by size:
2670 1784 1192 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 592 1071 1178 3452 3617 3644 3984 4250
Size: 592 479 107 2274 165 27 340 266
Cuts at: 4250 5342 5612 6705
Size: 1092 270 1093
Fragments arranged by size:
2274 1093 1092 592 479 340 270 266
165 107 27
BsrBI CCG'CTC
Cuts at: 0 1967 6705
Size: 1967 4738
BsrDI GCAATG_nn'
Cuts at: 0 3057 4391 4685 6705
Size: 3057 1334 294 2020
Fragments arranged by size:
3057 2020 1334 294
BsrFI r'CCGG_y
Cuts at: 0 65 1139 2279 4979 6705
Size: 65 1074 1140 2700 1726
Fragments arranged by size:
2700 1726 1140 1074 65
BssSI C'ACGA_G
Cuts at: 0 2564 4310 4541 6705
Size: 2564 1746 231 2164
Fragments arranged by size:
2564 2164 1746 231
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1052 6705
Size: 1052 5653
BstBI TT'CG_AA
Cuts at: 0 957 6705
Size: 957 5748
BstEII G'GTnAC_C
Cuts at: 0 1083 4164 5043 6415 6705
Size: 1083 3081 879 1372 290
Fragments arranged by size:
3081 1372 1083 879 290
BstXI CCAn_nnnn'nTGG
Cuts at: 0 337 3780 4681 5316 6705
Size: 337 3443 901 635 1389
Fragments arranged by size:
3443 1389 901 635 337
BstYI r'GATC_y
Cuts at: 0 152 383 3015 3104 3179 3303 3465 3783
Size: 152 231 2632 89 75 124 162 318
Cuts at: 3783 4320 5220 5405 6705
Size: 537 900 185 1300
Fragments arranged by size:
2632 1300 900 537 318 231 185 162
152 124 89 75
Bsu36I CC'TnA_GG
Cuts at: 0 1360 3005 3707 6705
Size: 1360 1645 702 2998
Fragments arranged by size:
2998 1645 1360 702
BtgI C'CryG_G
Cuts at: 0 2943 3088 3199 3408 3651 6482 6705
Size: 2943 145 111 209 243 2831 223
Fragments arranged by size:
2943 2831 243 223 209 145 111
BtsI GCAGTG_nn'
Cuts at: 0 650 1431 1818 2884 3221 3646 4825 5036
Size: 650 781 387 1066 337 425 1179 211
Cuts at: 5036 6705
Size: 1669
Fragments arranged by size:
1669 1179 1066 781 650 425 387 337
211
DraIII CAC_nnn'GTG
Cuts at: 0 95 255 4543 6294 6376 6705
Size: 95 160 4288 1751 82 329
Fragments arranged by size:
4288 1751 329 160 95 82
DrdI GACnn_nn'nnGTC
Cuts at: 0 1729 6705
Size: 1729 4976
EaeI y'GGCC_r
Cuts at: 0 86 2048 2744 3324 3455 4938 6093 6705
Size: 86 1962 696 580 131 1483 1155 612
Fragments arranged by size:
1962 1483 1155 696 612 580 131 86
EarI CTCTTCn'nnn_
Cuts at: 0 709 1689 2384 3916 4190 4616 4646 5971
Size: 709 980 695 1532 274 426 30 1325
Cuts at: 5971 6122 6614 6705
Size: 151 492 91
Fragments arranged by size:
1532 1325 980 709 695 492 426 274
151 91 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 5950 6705
Size: 133 5817 755
Fragments arranged by size:
5817 755 133
EcoICRI GAG'CTC
Cuts at: 0 2799 4302 5930 6705
Size: 2799 1503 1628 775
Fragments arranged by size:
2799 1628 1503 775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 595 799 928 1573 1760 1993 2108
Size: 348 247 204 129 645 187 233 115
Cuts at: 2108 2218 2563 4214 4483 4545 5213 5512 5557
Size: 110 345 1651 269 62 668 299 45
Cuts at: 5557 5878 5952 6463 6568 6705
Size: 321 74 511 105 137
Fragments arranged by size:
1651 668 645 511 348 345 321 299
269 247 233 204 187 137 129 115
110 105 74 62 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3706 4094 4159 5761 6705
Size: 3706 388 65 1602 944
Fragments arranged by size:
3706 1602 944 388 65
EcoO109I rG'GnC_Cy
Cuts at: 0 699 729 1899 3291 3690 4458 6214 6705
Size: 699 30 1170 1392 399 768 1756 491
Fragments arranged by size:
1756 1392 1170 768 699 491 399 30
EcoRI G'AATT_C
Cuts at: 0 1150 3821 6705
Size: 1150 2671 2884
Fragments arranged by size:
2884 2671 1150
EcoRV GAT'ATC
Cuts at: 0 3561 6705
Size: 3561 3144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1740 3570 6705
Size: 1740 1830 3135
Fragments arranged by size:
3135 1830 1740
HaeII r_GCGC'y
Cuts at: 0 53 338 1450 1858 4116 5757 6705
Size: 53 285 1112 408 2258 1641 948
Fragments arranged by size:
2258 1641 1112 948 408 285 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 888 920 2254 2286 3244 3276 4567 4599
Size: 888 32 1334 32 958 32 1291 32
Cuts at: 4599 6121 6153 6705
Size: 1522 32 552
Fragments arranged by size:
1522 1334 1291 958 888 552 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1284 3130 4548 4743 5328 6705
Size: 1284 1846 1418 195 585 1377
Fragments arranged by size:
1846 1418 1377 1284 585 195
HindIII A'AGCT_T
Cuts at: 0 960 5569 6705
Size: 960 4609 1136
Fragments arranged by size:
4609 1136 960
HpaI GTT'AAC
Cuts at: 0 4548 6705
Size: 4548 2157
KasI G'GCGC_C
Cuts at: 0 1446 4112 5753 6705
Size: 1446 2666 1641 952
Fragments arranged by size:
2666 1641 1446 952
KpnI G_GTAC'C
Cuts at: 0 3096 6705
Size: 3096 3609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5682 6705
Size: 5682 1023
MscI TGG'CCA
Cuts at: 0 88 2050 3326 3457 6705
Size: 88 1962 1276 131 3248
Fragments arranged by size:
3248 1962 1276 131 88
MslI CAynn'nnrTG
Cuts at: 0 413 859 935 1710 2872 3283 3674 3778
Size: 413 446 76 775 1162 411 391 104
Cuts at: 3778 4105 6465 6705
Size: 327 2360 240
Fragments arranged by size:
2360 1162 775 446 413 411 391 327
240 104 76
MspA1I CmG'CkG
Cuts at: 0 105 573 822 948 1383 1443 1669 2373
Size: 105 468 249 126 435 60 226 704
Cuts at: 2373 3358 3453 3990 4080 4291 4344 4429 4798
Size: 985 95 537 90 211 53 85 369
Cuts at: 4798 5074 5103 5490 5610 5667 5778 6048 6183
Size: 276 29 387 120 57 111 270 135
Cuts at: 6183 6705
Size: 522
Fragments arranged by size:
985 704 537 522 468 435 387 369
276 270 249 226 211 135 126 120
111 105 95 90 85 60 57 53
29
NaeI GCC'GGC
Cuts at: 0 2281 6705
Size: 2281 4424
NarI GG'CG_CC
Cuts at: 0 1447 4113 5754 6705
Size: 1447 2666 1641 951
Fragments arranged by size:
2666 1641 1447 951
NcoI C'CATG_G
Cuts at: 0 2943 3408 3651 6482 6705
Size: 2943 465 243 2831 223
Fragments arranged by size:
2943 2831 465 243 223
NgoMIV G'CCGG_C
Cuts at: 0 2279 6705
Size: 2279 4426
NheI G'CTAG_C
Cuts at: 0 1173 5952 6705
Size: 1173 4779 753
Fragments arranged by size:
4779 1173 753
NsiI A_TGCA'T
Cuts at: 0 1469 6705
Size: 1469 5236
NspI r_CATG'y
Cuts at: 0 1471 2028 2044 2959 3231 4605 4896 5473
Size: 1471 557 16 915 272 1374 291 577
Cuts at: 5473 6609 6705
Size: 1136 96
Fragments arranged by size:
1471 1374 1136 915 577 557 291 272
96 16
PasI CC'CwG_GG
Cuts at: 0 6217 6705
Size: 6217 488
PciI A'CATG_T
Cuts at: 0 2024 4601 6705
Size: 2024 2577 2104
Fragments arranged by size:
2577 2104 2024
PflMI CCAn_nnn'nTGG
Cuts at: 0 95 1245 1530 1657 3333 5060 5277 6705
Size: 95 1150 285 127 1676 1727 217 1428
Fragments arranged by size:
1727 1676 1428 1150 285 217 127 95
PfoI T'CCnGG_A
Cuts at: 0 4369 4870 5315 6705
Size: 4369 501 445 1390
Fragments arranged by size:
4369 1390 501 445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2227 2259 6705
Size: 2227 32 4446
Fragments arranged by size:
4446 2227 32
PshAI GACnn'nnGTC
Cuts at: 0 617 3880 4157 6705
Size: 617 3263 277 2548
Fragments arranged by size:
3263 2548 617 277
PspOMI G'GGCC_C
Cuts at: 0 729 3291 3689 6213 6705
Size: 729 2562 398 2524 492
Fragments arranged by size:
2562 2524 729 492 398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2954 2986 6705
Size: 2954 32 3719
Fragments arranged by size:
3719 2954 32
PstI C_TGCA'G
Cuts at: 0 116 920 2262 2300 2429 3641 3901 3995
Size: 116 804 1342 38 129 1212 260 94
Cuts at: 3995 4820 5609 5666 5804 6705
Size: 825 789 57 138 901
Fragments arranged by size:
1342 1212 901 825 804 789 260 138
129 116 94 57 38
PvuII CAG'CTG
Cuts at: 0 573 822 948 1669 2373 3358 3453 3990
Size: 573 249 126 721 704 985 95 537
Cuts at: 3990 4291 4344 4429 4798 5074 5490 5610 5778
Size: 301 53 85 369 276 416 120 168
Cuts at: 5778 6048 6183 6705
Size: 270 135 522
Fragments arranged by size:
985 721 704 573 537 522 416 369
301 276 270 249 168 135 126 120
95 85 53
SacI G_AGCT'C
Cuts at: 0 2801 4304 5932 6705
Size: 2801 1503 1628 773
Fragments arranged by size:
2801 1628 1503 773
SalI G'TCGA_C
Cuts at: 0 3128 6705
Size: 3128 3577
SapI GCTCTTCn'nnn_
Cuts at: 0 3916 6705
Size: 3916 2789
SbfI CC_TGCA'GG
Cuts at: 0 2300 6705
Size: 2300 4405
ScaI AGT'ACT
Cuts at: 0 4900 5915 6705
Size: 4900 1015 790
Fragments arranged by size:
4900 1015 790
SexAI A'CCwGG_T
Cuts at: 0 830 3528 6425 6705
Size: 830 2698 2897 280
Fragments arranged by size:
2897 2698 830 280
SfcI C'TryA_G
Cuts at: 0 112 295 916 1507 2056 2258 2296 2425
Size: 112 183 621 591 549 202 38 129
Cuts at: 2425 2722 3637 3897 3991 4816 5605 5662 5800
Size: 297 915 260 94 825 789 57 138
Cuts at: 5800 5814 6705
Size: 14 891
Fragments arranged by size:
915 891 825 789 621 591 549 297
260 202 183 138 129 112 94 57
38 14
SfoI GGC'GCC
Cuts at: 0 1448 4114 5755 6705
Size: 1448 2666 1641 950
Fragments arranged by size:
2666 1641 1448 950
SmlI C'TyrA_G
Cuts at: 0 169 436 1066 3043 4091 5200 5401 6705
Size: 169 267 630 1977 1048 1109 201 1304
Fragments arranged by size:
1977 1304 1109 1048 630 267 201 169
SpeI A'CTAG_T
Cuts at: 0 4522 6705
Size: 4522 2183
SphI G_CATG'C
Cuts at: 0 4896 6705
Size: 4896 1809
SspI AAT'ATT
Cuts at: 0 3269 6705
Size: 3269 3436
StuI AGG'CCT
Cuts at: 0 3891 6705
Size: 3891 2814
StyI C'CwwG_G
Cuts at: 0 1188 1322 1763 2943 3408 3651 3716 4223
Size: 1188 134 441 1180 465 243 65 507
Cuts at: 4223 6482 6705
Size: 2259 223
Fragments arranged by size:
2259 1188 1180 507 465 441 243 223
134 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1469 1473 1561 2886 4203 6705
Size: 1469 4 88 1325 1317 2502
Fragments arranged by size:
2502 1469 1325 1317 88 4
TatI w'GTAC_w
Cuts at: 0 3275 4431 4898 5132 5913 6705
Size: 3275 1156 467 234 781 792
Fragments arranged by size:
3275 1156 792 781 467 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1358 1390 1577 1609 1966 1998 2073 2105
Size: 1358 32 187 32 357 32 75 32
Cuts at: 2105 3450 3482 3625 3657 6149 6181 6705
Size: 1345 32 143 32 2492 32 524
Fragments arranged by size:
2492 1358 1345 524 357 187 143 75
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 832 5323 6705
Size: 832 4491 1382
Fragments arranged by size:
4491 1382 832
XbaI T'CTAG_A
Cuts at: 0 2511 6705
Size: 2511 4194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3377 5607 6705
Size: 3377 2230 1098
Fragments arranged by size:
3377 2230 1098
XmnI GAAnn'nnTTC
Cuts at: 0 385 2133 2413 2969 5852 6705
Size: 385 1748 280 556 2883 853
Fragments arranged by size:
2883 1748 853 556 385 280
ZraI GAC'GTC
Cuts at: 0 1823 6705
Size: 1823 4882
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PpiI
PshAI PspOMI PsrI PstI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AhdI ApaLI AscI AseI AsiSI AvrII
BaeI BglI BmgBI BsaAI BsiEI BsiWI BsrGI BssHII
BstZ17I BtgZI ClaI CspCI DraI EagI FseI FspI
FspAI MfeI MluI NdeI NotI NruI PacI PmeI
PmlI PpuMI PsiI PspXI PvuI RsrII SacII SanDI
SfiI SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI
XmaI