(Linear) (Six-base) MAPSORT of: KIAA0102.seq  Check: 27  from: 1  to: 678



KIAA0102.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:49  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     547     579     678 
   Size:        547      32      99

  Fragments arranged by size: 

                 547      99      32
 
ApoI r'AATT_y

Cuts at:      0     228     678 
   Size:        228     450
 
BamHI G'GATC_C

Cuts at:      0     453     678 
   Size:        453     225
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     402     436     678 
   Size:        402      34     242

  Fragments arranged by size: 

                 402     242      34
 
BfrBI ATG'CAT

Cuts at:      0     619     678 
   Size:        619      59
 
BglI GCCn_nnn'nGGC

Cuts at:      0      98     289     678 
   Size:         98     191     389

  Fragments arranged by size: 

                 389     191      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465     678 
   Size:        465     213
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      18      48     678 
   Size:         18      30     630

  Fragments arranged by size: 

                 630      30      18
 
BsrDI GCAATG_nn'

Cuts at:      0     575     678 
   Size:        575     103
 
BsrGI T'GTAC_A

Cuts at:      0      15     262     678 
   Size:         15     247     416

  Fragments arranged by size: 

                 416     247      15
 
BssSI C'ACGA_G

Cuts at:      0     421     678 
   Size:        421     257
 
BstYI r'GATC_y

Cuts at:      0     438     453     678 
   Size:        438      15     225

  Fragments arranged by size: 

                 438     225      15
 
EaeI y'GGCC_r

Cuts at:      0      90     678 
   Size:         90     588
 
EarI CTCTTCn'nnn_

Cuts at:      0     403     678 
   Size:        403     275
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465     678 
   Size:        465     213
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     432     540     678 
   Size:        432     108     138

  Fragments arranged by size: 

                 432     138     108
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86      95     678 
   Size:         86       9     583

  Fragments arranged by size: 

                 583      86       9
 
MspA1I CmG'CkG

Cuts at:      0      13     163     474     552     678 
   Size:         13     150     311      78     126

  Fragments arranged by size: 

                 311     150     126      78      13
 
NdeI CA'TA_TG

Cuts at:      0     621     678 
   Size:        621      57
 
NsiI A_TGCA'T

Cuts at:      0     621     678 
   Size:        621      57
 
PshAI GACnn'nnGTC

Cuts at:      0     607     678 
   Size:        607      71
 
PvuII CAG'CTG

Cuts at:      0      13     163     474     678 
   Size:         13     150     311     204

  Fragments arranged by size: 

                 311     204     150      13
 
SapI GCTCTTCn'nnn_

Cuts at:      0     403     678 
   Size:        403     275
 
SfcI C'TryA_G

Cuts at:      0      48     443     678 
   Size:         48     395     235

  Fragments arranged by size: 

                 395     235      48
 
TatI w'GTAC_w

Cuts at:      0      15     262     678 
   Size:         15     247     416

  Fragments arranged by size: 

                 416     247      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      18      50      72     104     149     181     678 
   Size:         18      32      22      32      45      32     497

  Fragments arranged by size: 

                 497      45      32      32      32      22      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     336     678 
   Size:        336     342
 

 Enzymes that do cut:

     AloI     ApoI    BamHI     BcgI    BfrBI     BglI     BpmI    BsaXI
    BsrDI    BsrGI    BssSI    BstYI     EaeI     EarI  Eco57MI     FalI
     MmeI   MspA1I     NdeI     NsiI    PshAI    PvuII     SapI     SfcI
     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI