Restriction map for pF1KA0103
 (Linear) (Six-base) MAPSORT of: KIAA0103.seq  Check: 2224  from: 1  to: 891



KIAA0103.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AccI GT'mk_AC

Cuts at:      0     598     891 
   Size:        598     293
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608     891 
   Size:        608     283
 
AflII C'TTAA_G

Cuts at:      0     844     891 
   Size:        844      47
 
AflIII A'CryG_T

Cuts at:      0     860     891 
   Size:        860      31
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     497     891 
   Size:        497     394
 
AseI AT'TA_AT

Cuts at:      0     118     122     891 
   Size:        118       4     769

  Fragments arranged by size: 

                 769     118       4
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     217     246     863     891 
   Size:        217      29     617      28

  Fragments arranged by size: 

                 617     217      29      28
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     879     891 
   Size:        879      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     891 
   Size:         63     828
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      15     452     891 
   Size:         15     437     439

  Fragments arranged by size: 

                 439     437      15
 
BseYI C'CCAG_C

Cuts at:      0     141     891 
   Size:        141     750
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     802     891 
   Size:        802      89
 
BsrDI GCAATG_nn'

Cuts at:      0     871     891 
   Size:        871      20
 
BstBI TT'CG_AA

Cuts at:      0     390     891 
   Size:        390     501
 
BstYI r'GATC_y

Cuts at:      0     348     891 
   Size:        348     543
 
BstZ17I GTA'TAC

Cuts at:      0     599     891 
   Size:        599     292
 
BtgI C'CryG_G

Cuts at:      0     296     891 
   Size:        296     595
 
BtsI GCAGTG_nn'

Cuts at:      0     359     891 
   Size:        359     532
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608     891 
   Size:        608     283
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     628     891 
   Size:        628     263
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     537     569     891 
   Size:        537      32     322

  Fragments arranged by size: 

                 537     322      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     376     435     845     891 
   Size:         87     289      59     410      46

  Fragments arranged by size: 

                 410     289      87      59      46
 
MslI CAynn'nnrTG

Cuts at:      0     509     891 
   Size:        509     382
 
NcoI C'CATG_G

Cuts at:      0     296     891 
   Size:        296     595
 
NspI r_CATG'y

Cuts at:      0     864     891 
   Size:        864      27
 
PacI TTA_AT'TAA

Cuts at:      0     122     891 
   Size:        122     769
 
PasI CC'CwG_GG

Cuts at:      0     399     891 
   Size:        399     492
 
PciI A'CATG_T

Cuts at:      0     860     891 
   Size:        860      31
 
PshAI GACnn'nnGTC

Cuts at:      0     197     891 
   Size:        197     694
 
SmlI C'TyrA_G

Cuts at:      0      78     844     891 
   Size:         78     766      47

  Fragments arranged by size: 

                 766      78      47
 
SspI AAT'ATT

Cuts at:      0     838     891 
   Size:        838      53
 
StyI C'CwwG_G

Cuts at:      0     296     603     891 
   Size:        296     307     288

  Fragments arranged by size: 

                 307     296     288
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AlfI     AseI     BbsI     BmrI
    BpuEI     BsaI    BseYI    BspMI    BsrDI    BstBI    BstYI  BstZ17I
     BtgI     BtsI  Eco57MI     FalI    Hin4I     MmeI     MslI     NcoI
     NspI     PacI     PasI     PciI    PshAI     SmlI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com