Restriction map for
pF1KA0104
(Linear) (Six-base) MAPSORT of: KIAA0104.seq Check: 1272 from: 1 to: 876
KIAA0104.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AgeI A'CCGG_T
Cuts at: 0 177 876
Size: 177 699
AhdI GACnn_n'nnGTC
Cuts at: 0 475 876
Size: 475 401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 47 876
Size: 47 829
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 876
Size: 205 32 639
Fragments arranged by size:
639 205 32
AlwNI CAG_nnn'CTG
Cuts at: 0 399 876
Size: 399 477
ApoI r'AATT_y
Cuts at: 0 235 271 708 796 876
Size: 235 36 437 88 80
Fragments arranged by size:
437 235 88 80 36
AvaI C'yCGr_G
Cuts at: 0 506 876
Size: 506 370
AvrII C'CTAG_G
Cuts at: 0 42 876
Size: 42 834
BanII G_rGCy'C
Cuts at: 0 152 876
Size: 152 724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 876
Size: 136 34 706
Fragments arranged by size:
706 136 34
BglI GCCn_nnn'nGGC
Cuts at: 0 123 876
Size: 123 753
Bme1580I G_kGCm'C
Cuts at: 0 26 876
Size: 26 850
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 146 876
Size: 36 110 730
Fragments arranged by size:
730 110 36
Bpu10I CC'TnA_GC
Cuts at: 0 677 876
Size: 677 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 876
Size: 246 630
BsaWI w'CCGG_w
Cuts at: 0 177 210 876
Size: 177 33 666
Fragments arranged by size:
666 177 33
BseYI C'CCAG_C
Cuts at: 0 695 876
Size: 695 181
BsiHKAI G_wGCw'C
Cuts at: 0 136 876
Size: 136 740
BsmI GAATG_Cn'
Cuts at: 0 410 876
Size: 410 466
Bsp1286I G_dGCh'C
Cuts at: 0 26 136 152 876
Size: 26 110 16 724
Fragments arranged by size:
724 110 26 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 876
Size: 209 667
BsrDI GCAATG_nn'
Cuts at: 0 11 41 876
Size: 11 30 835
Fragments arranged by size:
835 30 11
BsrFI r'CCGG_y
Cuts at: 0 84 177 876
Size: 84 93 699
Fragments arranged by size:
699 93 84
BstYI r'GATC_y
Cuts at: 0 261 876
Size: 261 615
Bsu36I CC'TnA_GG
Cuts at: 0 451 876
Size: 451 425
BtgI C'CryG_G
Cuts at: 0 203 787 876
Size: 203 584 89
Fragments arranged by size:
584 203 89
EarI CTCTTCn'nnn_
Cuts at: 0 420 876
Size: 420 456
EcoO109I rG'GnC_Cy
Cuts at: 0 115 876
Size: 115 761
EcoRI G'AATT_C
Cuts at: 0 235 876
Size: 235 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 461 493 876
Size: 461 32 383
Fragments arranged by size:
461 383 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 318 876
Size: 186 132 558
Fragments arranged by size:
558 186 132
MspA1I CmG'CkG
Cuts at: 0 19 364 876
Size: 19 345 512
Fragments arranged by size:
512 345 19
NaeI GCC'GGC
Cuts at: 0 86 876
Size: 86 790
NcoI C'CATG_G
Cuts at: 0 787 876
Size: 787 89
NdeI CA'TA_TG
Cuts at: 0 372 876
Size: 372 504
NgoMIV G'CCGG_C
Cuts at: 0 84 876
Size: 84 792
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 876
Size: 399 477
PfoI T'CCnGG_A
Cuts at: 0 512 876
Size: 512 364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 876
Size: 249 32 595
Fragments arranged by size:
595 249 32
PsiI TTA'TAA
Cuts at: 0 500 876
Size: 500 376
PstI C_TGCA'G
Cuts at: 0 103 876
Size: 103 773
PvuII CAG'CTG
Cuts at: 0 364 876
Size: 364 512
SbfI CC_TGCA'GG
Cuts at: 0 103 876
Size: 103 773
ScaI AGT'ACT
Cuts at: 0 314 876
Size: 314 562
SfcI C'TryA_G
Cuts at: 0 99 359 876
Size: 99 260 517
Fragments arranged by size:
517 260 99
SmlI C'TyrA_G
Cuts at: 0 225 876
Size: 225 651
SspI AAT'ATT
Cuts at: 0 717 876
Size: 717 159
StyI C'CwwG_G
Cuts at: 0 42 787 876
Size: 42 745 89
Fragments arranged by size:
745 89 42
TatI w'GTAC_w
Cuts at: 0 312 621 876
Size: 312 309 255
Fragments arranged by size:
312 309 255
XmnI GAAnn'nnTTC
Cuts at: 0 239 876
Size: 239 637
Enzymes that do cut:
AgeI AhdI AlfI AloI AlwNI ApoI AvaI AvrII
BanII BcgI BglI Bme1580I BmrI Bpu10I BpuEI BsaWI
BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstYI
Bsu36I BtgI EarI EcoO109I EcoRI Hin4I MmeI MspA1I
NaeI NcoI NdeI NgoMIV PflMI PfoI PpiI PsiI
PstI PvuII SbfI ScaI SfcI SmlI SspI StyI
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NarI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI