Restriction map for
pF1KA0107
(Linear) (Six-base) MAPSORT of: KIAA0107.seq Check: 5192 from: 1 to: 1167
KIAA0107.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 777 921 999 1167
Size: 777 144 78 168
Fragments arranged by size:
777 168 144 78
ApoI r'AATT_y
Cuts at: 0 296 899 982 1167
Size: 296 603 83 185
Fragments arranged by size:
603 296 185 83
AseI AT'TA_AT
Cuts at: 0 1160 1167
Size: 1160 7
AvaI C'yCGr_G
Cuts at: 0 85 1167
Size: 85 1082
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 36 69 1167
Size: 36 33 1098
Fragments arranged by size:
1098 36 33
BanII G_rGCy'C
Cuts at: 0 360 1167
Size: 360 807
BbsI GAAGACnn'nnnn_
Cuts at: 0 748 1167
Size: 748 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 196 1167
Size: 162 34 971
Fragments arranged by size:
971 162 34
BclI T'GATC_A
Cuts at: 0 990 1167
Size: 990 177
BfrBI ATG'CAT
Cuts at: 0 907 1167
Size: 907 260
BglII A'GATC_T
Cuts at: 0 702 1119 1167
Size: 702 417 48
Fragments arranged by size:
702 417 48
BlpI GC'TnA_GC
Cuts at: 0 76 1167
Size: 76 1091
BmgBI CAC'GTC
Cuts at: 0 198 1167
Size: 198 969
BmrI ACTGGGnnnn_n'
Cuts at: 0 528 1167
Size: 528 639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 37 1167
Size: 28 9 1130
Fragments arranged by size:
1130 28 9
BsaBI GATnn'nnATC
Cuts at: 0 461 1167
Size: 461 706
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 271 527 1167
Size: 34 3 234 256 640
Fragments arranged by size:
640 256 234 34 3
BsiEI CG_ry'CG
Cuts at: 0 131 1167
Size: 131 1036
BsiHKAI G_wGCw'C
Cuts at: 0 92 360 1167
Size: 92 268 807
Fragments arranged by size:
807 268 92
BsmI GAATG_Cn'
Cuts at: 0 799 1167
Size: 799 368
BsmBI CGTCTCn'nnnn_
Cuts at: 0 92 1167
Size: 92 1075
Bsp1286I G_dGCh'C
Cuts at: 0 92 360 1167
Size: 92 268 807
Fragments arranged by size:
807 268 92
BsrDI GCAATG_nn'
Cuts at: 0 314 1167
Size: 314 853
BstEII G'GTnAC_C
Cuts at: 0 403 1167
Size: 403 764
BstYI r'GATC_y
Cuts at: 0 702 1119 1167
Size: 702 417 48
Fragments arranged by size:
702 417 48
Bsu36I CC'TnA_GG
Cuts at: 0 433 1167
Size: 433 734
BtgI C'CryG_G
Cuts at: 0 93 1167
Size: 93 1074
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 317 1167
Size: 317 850
BtsI GCAGTG_nn'
Cuts at: 0 1029 1167
Size: 1029 138
ClaI AT'CG_AT
Cuts at: 0 876 1167
Size: 876 291
EaeI y'GGCC_r
Cuts at: 0 128 1167
Size: 128 1039
EagI C'GGCC_G
Cuts at: 0 128 1167
Size: 128 1039
EarI CTCTTCn'nnn_
Cuts at: 0 604 1167
Size: 604 563
EcoICRI GAG'CTC
Cuts at: 0 358 1167
Size: 358 809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 37 1167
Size: 28 9 1130
Fragments arranged by size:
1130 28 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 1167
Size: 19 1148
EcoRI G'AATT_C
Cuts at: 0 899 982 1167
Size: 899 83 185
Fragments arranged by size:
899 185 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 1167
Size: 689 32 446
Fragments arranged by size:
689 446 32
HindIII A'AGCT_T
Cuts at: 0 495 1167
Size: 495 672
MspA1I CmG'CkG
Cuts at: 0 6 63 95 592 918 1167
Size: 6 57 32 497 326 249
Fragments arranged by size:
497 326 249 57 32 6
NdeI CA'TA_TG
Cuts at: 0 381 1167
Size: 381 786
NruI TCG'CGA
Cuts at: 0 302 1167
Size: 302 865
NsiI A_TGCA'T
Cuts at: 0 909 1167
Size: 909 258
PasI CC'CwG_GG
Cuts at: 0 399 1167
Size: 399 768
PsiI TTA'TAA
Cuts at: 0 647 1167
Size: 647 520
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1167
Size: 1078 32 57
Fragments arranged by size:
1078 57 32
PvuII CAG'CTG
Cuts at: 0 63 592 918 1167
Size: 63 529 326 249
Fragments arranged by size:
529 326 249 63
SacI G_AGCT'C
Cuts at: 0 360 1167
Size: 360 807
SacII CC_GC'GG
Cuts at: 0 96 1167
Size: 96 1071
StyI C'CwwG_G
Cuts at: 0 945 1167
Size: 945 222
Enzymes that do cut:
AlwNI ApoI AseI AvaI BaeI BanII BbsI BcgI
BclI BfrBI BglII BlpI BmgBI BmrI BpmI BsaBI
BseRI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoRI Hin4I HindIII MspA1I
NdeI NruI NsiI PasI PsiI PsrI PvuII SacI
SacII StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AsiSI AvrII BamHI BanI BbeI BbvCI BciVI
BglI Bme1580I BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiWI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I CspCI DraI DraIII DrdI EciI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NgoMIV NheI NotI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI