Restriction map for
pF1KA0109
(Linear) (Six-base) MAPSORT of: KIAA0109.seq Check: 613 from: 1 to: 1305
KIAA0109.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 114 558 990 1305
Size: 114 444 432 315
Fragments arranged by size:
444 432 315 114
AccI GT'mk_AC
Cuts at: 0 56 1305
Size: 56 1249
AleI CACnn'nnGTG
Cuts at: 0 749 1305
Size: 749 556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 737 1305
Size: 737 568
AlwNI CAG_nnn'CTG
Cuts at: 0 573 1305
Size: 573 732
ApoI r'AATT_y
Cuts at: 0 238 366 1305
Size: 238 128 939
Fragments arranged by size:
939 238 128
AvaI C'yCGr_G
Cuts at: 0 50 1305
Size: 50 1255
BamHI G'GATC_C
Cuts at: 0 968 1305
Size: 968 337
BanI G'GyrC_C
Cuts at: 0 1173 1305
Size: 1173 132
BanII G_rGCy'C
Cuts at: 0 518 1146 1305
Size: 518 628 159
Fragments arranged by size:
628 518 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 1305
Size: 225 1080
BglI GCCn_nnn'nGGC
Cuts at: 0 1283 1305
Size: 1283 22
Bme1580I G_kGCm'C
Cuts at: 0 582 1305
Size: 582 723
BmgBI CAC'GTC
Cuts at: 0 261 1305
Size: 261 1044
BmrI ACTGGGnnnn_n'
Cuts at: 0 476 1305
Size: 476 829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 922 1305
Size: 553 369 383
Fragments arranged by size:
553 383 369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1305
Size: 1180 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 1018 1305
Size: 720 298 287
Fragments arranged by size:
720 298 287
BsiHKAI G_wGCw'C
Cuts at: 0 44 320 518 1305
Size: 44 276 198 787
Fragments arranged by size:
787 276 198 44
BsmI GAATG_Cn'
Cuts at: 0 637 1050 1305
Size: 637 413 255
Fragments arranged by size:
637 413 255
Bsp1286I G_dGCh'C
Cuts at: 0 44 320 518 582 1146 1305
Size: 44 276 198 64 564 159
Fragments arranged by size:
564 276 198 159 64 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 554 558 990 1305
Size: 114 440 4 432 315
Fragments arranged by size:
440 432 315 114 4
BsrBI CCG'CTC
Cuts at: 0 707 1305
Size: 707 598
BsrDI GCAATG_nn'
Cuts at: 0 146 1305
Size: 146 1159
BssHII G'CGCG_C
Cuts at: 0 380 1305
Size: 380 925
BstAPI GCAn_nnn'nTGC
Cuts at: 0 88 126 1305
Size: 88 38 1179
Fragments arranged by size:
1179 88 38
BstBI TT'CG_AA
Cuts at: 0 236 1305
Size: 236 1069
BstXI CCAn_nnnn'nTGG
Cuts at: 0 466 1305
Size: 466 839
BstYI r'GATC_y
Cuts at: 0 968 1305
Size: 968 337
BtgI C'CryG_G
Cuts at: 0 227 1305
Size: 227 1078
BtsI GCAGTG_nn'
Cuts at: 0 89 940 1287 1305
Size: 89 851 347 18
Fragments arranged by size:
851 347 89 18
DraI TTT'AAA
Cuts at: 0 933 1305
Size: 933 372
DrdI GACnn_nn'nnGTC
Cuts at: 0 1249 1305
Size: 1249 56
EaeI y'GGCC_r
Cuts at: 0 1275 1305
Size: 1275 30
EarI CTCTTCn'nnn_
Cuts at: 0 431 523 601 1305
Size: 431 92 78 704
Fragments arranged by size:
704 431 92 78
EcoICRI GAG'CTC
Cuts at: 0 516 1305
Size: 516 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 922 1305
Size: 553 369 383
Fragments arranged by size:
553 383 369
EcoRI G'AATT_C
Cuts at: 0 238 366 1305
Size: 238 128 939
Fragments arranged by size:
939 238 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 945 1305
Size: 945 360
FspI TGC'GCA
Cuts at: 0 130 1305
Size: 130 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 408 440 479 511 1305
Size: 53 32 323 32 39 32 794
Fragments arranged by size:
794 323 53 39 32 32 32
HincII GTy'rAC
Cuts at: 0 219 1305
Size: 219 1086
MfeI C'AATT_G
Cuts at: 0 719 1305
Size: 719 586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 487 950 1305
Size: 206 281 463 355
Fragments arranged by size:
463 355 281 206
MslI CAynn'nnrTG
Cuts at: 0 749 1159 1305
Size: 749 410 146
Fragments arranged by size:
749 410 146
MspA1I CmG'CkG
Cuts at: 0 688 1305
Size: 688 617
NcoI C'CATG_G
Cuts at: 0 227 1305
Size: 227 1078
NspI r_CATG'y
Cuts at: 0 627 1305
Size: 627 678
PflMI CCAn_nnn'nTGG
Cuts at: 0 188 1055 1305
Size: 188 867 250
Fragments arranged by size:
867 250 188
PfoI T'CCnGG_A
Cuts at: 0 521 1305
Size: 521 784
PvuII CAG'CTG
Cuts at: 0 688 1305
Size: 688 617
SacI G_AGCT'C
Cuts at: 0 518 1305
Size: 518 787
SapI GCTCTTCn'nnn_
Cuts at: 0 431 523 601 1305
Size: 431 92 78 704
Fragments arranged by size:
704 431 92 78
SfcI C'TryA_G
Cuts at: 0 1236 1305
Size: 1236 69
SmlI C'TyrA_G
Cuts at: 0 1195 1305
Size: 1195 110
SphI G_CATG'C
Cuts at: 0 627 1305
Size: 627 678
StyI C'CwwG_G
Cuts at: 0 227 839 1305
Size: 227 612 466
Fragments arranged by size:
612 466 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1251 1305
Size: 1251 54
TatI w'GTAC_w
Cuts at: 0 1028 1305
Size: 1028 277
XbaI T'CTAG_A
Cuts at: 0 345 1305
Size: 345 960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 1305
Size: 189 1116
XmnI GAAnn'nnTTC
Cuts at: 0 391 1305
Size: 391 914
Enzymes that do cut:
AarI AccI AleI AlfI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BglI Bme1580I BmgBI BmrI BpmI
BpuEI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BssHII BstAPI BstBI BstXI BstYI BtgI BtsI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoRI FalI FspI
Hin4I HincII MfeI MmeI MslI MspA1I NcoI NspI
PflMI PfoI PvuII SacI SapI SfcI SmlI SphI
StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BsrFI BsrGI BssSI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII EagI EciI EcoNI EcoO109I EcoRV
FseI FspAI HaeII HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI ZraI