Restriction map for
pF1KSDA0112
(Linear) (Six-base) MAPSORT of: KIAA0112.seq Check: 1872 from: 1 to: 1095
KIAA0112.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 91 1095
Size: 91 1004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 493 525 1095
Size: 493 32 570
Fragments arranged by size:
570 493 32
AlwNI CAG_nnn'CTG
Cuts at: 0 189 1095
Size: 189 906
ApaI G_GGCC'C
Cuts at: 0 979 1095
Size: 979 116
ApaLI G'TGCA_C
Cuts at: 0 76 1095
Size: 76 1019
ApoI r'AATT_y
Cuts at: 0 1026 1095
Size: 1026 69
AvaI C'yCGr_G
Cuts at: 0 446 734 1095
Size: 446 288 361
Fragments arranged by size:
446 361 288
BanI G'GyrC_C
Cuts at: 0 477 729 1095
Size: 477 252 366
Fragments arranged by size:
477 366 252
BanII G_rGCy'C
Cuts at: 0 284 742 979 1095
Size: 284 458 237 116
Fragments arranged by size:
458 284 237 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 388 1095
Size: 388 707
BbvCI CC'TCA_GC
Cuts at: 0 839 1095
Size: 839 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 129 163 1095
Size: 129 34 932
Fragments arranged by size:
932 129 34
BglI GCCn_nnn'nGGC
Cuts at: 0 362 926 1095
Size: 362 564 169
Fragments arranged by size:
564 362 169
Bme1580I G_kGCm'C
Cuts at: 0 80 482 734 979 1095
Size: 80 402 252 245 116
Fragments arranged by size:
402 252 245 116 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1095
Size: 109 986
Bpu10I CC'TnA_GC
Cuts at: 0 839 1095
Size: 839 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1095
Size: 343 752
BsaI GGTCTCn'nnnn_
Cuts at: 0 684 1095
Size: 684 411
BsaHI Gr'CG_yC
Cuts at: 0 121 1095
Size: 121 974
BsaWI w'CCGG_w
Cuts at: 0 128 628 1095
Size: 128 500 467
Fragments arranged by size:
500 467 128
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 493 523 1095
Size: 82 30 381 30 572
Fragments arranged by size:
572 381 82 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 661 895 907 921 1095
Size: 34 627 234 12 14 174
Fragments arranged by size:
627 234 174 34 14 12
BseYI C'CCAG_C
Cuts at: 0 428 604 651 982 1095
Size: 428 176 47 331 113
Fragments arranged by size:
428 331 176 113 47
BsiEI CG_ry'CG
Cuts at: 0 143 322 614 1095
Size: 143 179 292 481
Fragments arranged by size:
481 292 179 143
BsiHKAI G_wGCw'C
Cuts at: 0 80 1095
Size: 80 1015
Bsp1286I G_dGCh'C
Cuts at: 0 80 284 482 734 742 979 1095
Size: 80 204 198 252 8 237 116
Fragments arranged by size:
252 237 204 198 116 80 8
BspHI T'CATG_A
Cuts at: 0 821 1095
Size: 821 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 1095
Size: 122 973
BsrDI GCAATG_nn'
Cuts at: 0 492 1095
Size: 492 603
BsrFI r'CCGG_y
Cuts at: 0 563 613 944 1095
Size: 563 50 331 151
Fragments arranged by size:
563 331 151 50
BssHII G'CGCG_C
Cuts at: 0 352 441 584 1095
Size: 352 89 143 511
Fragments arranged by size:
511 352 143 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 594 1095
Size: 594 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 669 1095
Size: 669 426
BtgI C'CryG_G
Cuts at: 0 239 322 1095
Size: 239 83 773
Fragments arranged by size:
773 239 83
EaeI y'GGCC_r
Cuts at: 0 325 411 545 611 671 1095
Size: 325 86 134 66 60 424
Fragments arranged by size:
424 325 134 86 66 60
EagI C'GGCC_G
Cuts at: 0 611 1095
Size: 611 484
EarI CTCTTCn'nnn_
Cuts at: 0 248 991 1095
Size: 248 743 104
Fragments arranged by size:
743 248 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1095
Size: 109 986
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 1095
Size: 718 377
EcoO109I rG'GnC_Cy
Cuts at: 0 186 952 975 1095
Size: 186 766 23 120
Fragments arranged by size:
766 186 120 23
EcoRI G'AATT_C
Cuts at: 0 1026 1095
Size: 1026 69
HaeII r_GCGC'y
Cuts at: 0 429 702 714 1074 1095
Size: 429 273 12 360 21
Fragments arranged by size:
429 360 273 21 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 785 1095
Size: 104 681 310
Fragments arranged by size:
681 310 104
MscI TGG'CCA
Cuts at: 0 413 547 673 1095
Size: 413 134 126 422
Fragments arranged by size:
422 413 134 126
MslI CAynn'nnrTG
Cuts at: 0 73 667 1095
Size: 73 594 428
Fragments arranged by size:
594 428 73
MspA1I CmG'CkG
Cuts at: 0 225 234 324 600 843 1095
Size: 225 9 90 276 243 252
Fragments arranged by size:
276 252 243 225 90 9
NaeI GCC'GGC
Cuts at: 0 615 946 1095
Size: 615 331 149
Fragments arranged by size:
615 331 149
NgoMIV G'CCGG_C
Cuts at: 0 613 944 1095
Size: 613 331 151
Fragments arranged by size:
613 331 151
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 464 1095
Size: 15 449 631
Fragments arranged by size:
631 449 15
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 1095
Size: 493 32 570
Fragments arranged by size:
570 493 32
PspOMI G'GGCC_C
Cuts at: 0 975 1095
Size: 975 120
PstI C_TGCA'G
Cuts at: 0 98 1095
Size: 98 997
PvuII CAG'CTG
Cuts at: 0 225 234 600 843 1095
Size: 225 9 366 243 252
Fragments arranged by size:
366 252 243 225 9
RsrII CG'GwC_CG
Cuts at: 0 126 1095
Size: 126 969
SacII CC_GC'GG
Cuts at: 0 325 1095
Size: 325 770
SexAI A'CCwGG_T
Cuts at: 0 389 1095
Size: 389 706
SfcI C'TryA_G
Cuts at: 0 94 181 1095
Size: 94 87 914
Fragments arranged by size:
914 94 87
SmaI CCC'GGG
Cuts at: 0 448 736 1095
Size: 448 288 359
Fragments arranged by size:
448 359 288
SmlI C'TyrA_G
Cuts at: 0 358 1095
Size: 358 737
StyI C'CwwG_G
Cuts at: 0 497 674 719 1095
Size: 497 177 45 376
Fragments arranged by size:
497 376 177 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1036 1095
Size: 1036 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 335 690 1095
Size: 246 89 355 405
Fragments arranged by size:
405 355 246 89
XmaI C'CCGG_G
Cuts at: 0 446 734 1095
Size: 446 288 361
Fragments arranged by size:
446 361 288
Enzymes that do cut:
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BglI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BssHII BstAPI
BstXI BtgI EaeI EagI EarI Eco57MI EcoNI EcoO109I
EcoRI HaeII MmeI MscI MslI MspA1I NaeI NgoMIV
PflMI PpiI PspOMI PstI PvuII RsrII SacII SexAI
SfcI SmaI SmlI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaAI BsaBI BsgI BsiWI
BsmI BsmBI BspEI BsrBI BsrGI BssSI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI SacI SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI