Restriction map for
pF1KSDA0115
(Linear) (Six-base) MAPSORT of: KIAA0115.seq Check: 9770 from: 1 to: 1368
KIAA0115.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 10 1368
Size: 10 1358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 562 753 774 1123 1308 1368
Size: 220 342 191 21 349 185 60
Fragments arranged by size:
349 342 220 191 185 60 21
AfeI AGC'GCT
Cuts at: 0 1258 1368
Size: 1258 110
AflIII A'CryG_T
Cuts at: 0 1148 1368
Size: 1148 220
AleI CACnn'nnGTG
Cuts at: 0 142 1368
Size: 142 1226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 1368
Size: 160 1208
AlwNI CAG_nnn'CTG
Cuts at: 0 25 1368
Size: 25 1343
ApaI G_GGCC'C
Cuts at: 0 84 1368
Size: 84 1284
ApoI r'AATT_y
Cuts at: 0 271 1368
Size: 271 1097
AvaI C'yCGr_G
Cuts at: 0 71 1368
Size: 71 1297
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1205 1238 1368
Size: 1205 33 130
Fragments arranged by size:
1205 130 33
BanI G'GyrC_C
Cuts at: 0 828 1368
Size: 828 540
BanII G_rGCy'C
Cuts at: 0 59 84 221 652 740 1316 1368
Size: 59 25 137 431 88 576 52
Fragments arranged by size:
576 431 137 88 59 52 25
BbeI G_GCGC'C
Cuts at: 0 832 1368
Size: 832 536
BbsI GAAGACnn'nnnn_
Cuts at: 0 1326 1368
Size: 1326 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 707 1233 1368
Size: 707 526 135
Fragments arranged by size:
707 526 135
Bme1580I G_kGCm'C
Cuts at: 0 84 1368
Size: 84 1284
BmgBI CAC'GTC
Cuts at: 0 1149 1368
Size: 1149 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 687 1368
Size: 687 681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1084 1222 1368
Size: 724 360 138 146
Fragments arranged by size:
724 360 146 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 1368
Size: 334 1034
BsaHI Gr'CG_yC
Cuts at: 0 829 1368
Size: 829 539
BsaWI w'CCGG_w
Cuts at: 0 11 191 1368
Size: 11 180 1177
Fragments arranged by size:
1177 180 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 359 389 1368
Size: 359 30 979
Fragments arranged by size:
979 359 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 466 715 919 1368
Size: 57 409 249 204 449
Fragments arranged by size:
449 409 249 204 57
BseYI C'CCAG_C
Cuts at: 0 25 58 244 420 733 890 1308 1368
Size: 25 33 186 176 313 157 418 60
Fragments arranged by size:
418 313 186 176 157 60 33 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 838 1368
Size: 38 800 530
Fragments arranged by size:
800 530 38
BsiHKAI G_wGCw'C
Cuts at: 0 221 1368
Size: 221 1147
Bsp1286I G_dGCh'C
Cuts at: 0 59 84 221 652 740 1316 1368
Size: 59 25 137 431 88 576 52
Fragments arranged by size:
576 431 137 88 59 52 25
BspEI T'CCGG_A
Cuts at: 0 191 1368
Size: 191 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 545 1368
Size: 10 535 823
Fragments arranged by size:
823 535 10
BsrDI GCAATG_nn'
Cuts at: 0 728 1368
Size: 728 640
BsrFI r'CCGG_y
Cuts at: 0 1184 1368
Size: 1184 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 431 1368
Size: 431 937
BstEII G'GTnAC_C
Cuts at: 0 403 1368
Size: 403 965
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1308 1368
Size: 1308 60
BtgI C'CryG_G
Cuts at: 0 65 1368
Size: 65 1303
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1064 1368
Size: 1064 304
BtsI GCAGTG_nn'
Cuts at: 0 378 432 821 1368
Size: 378 54 389 547
Fragments arranged by size:
547 389 378 54
DraI TTT'AAA
Cuts at: 0 1170 1368
Size: 1170 198
EaeI y'GGCC_r
Cuts at: 0 501 608 1231 1368
Size: 501 107 623 137
Fragments arranged by size:
623 501 137 107
EarI CTCTTCn'nnn_
Cuts at: 0 416 657 1215 1321 1368
Size: 416 241 558 106 47
Fragments arranged by size:
558 416 241 106 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 380 1368
Size: 380 988
EcoICRI GAG'CTC
Cuts at: 0 219 1368
Size: 219 1149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 562 724 753 774 1084 1123 1222
Size: 220 342 162 29 21 310 39 99
Cuts at: 1222 1308 1368
Size: 86 60
Fragments arranged by size:
342 310 220 162 99 86 60 39
29 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 36 541 1368
Size: 36 505 827
Fragments arranged by size:
827 505 36
EcoO109I rG'GnC_Cy
Cuts at: 0 29 81 546 925 1035 1094 1368
Size: 29 52 465 379 110 59 274
Fragments arranged by size:
465 379 274 110 59 52 29
EcoRI G'AATT_C
Cuts at: 0 271 1368
Size: 271 1097
HaeII r_GCGC'y
Cuts at: 0 832 1260 1295 1368
Size: 832 428 35 73
Fragments arranged by size:
832 428 73 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 1368
Size: 314 32 1022
Fragments arranged by size:
1022 314 32
KasI G'GCGC_C
Cuts at: 0 828 1368
Size: 828 540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1368
Size: 419 949
MscI TGG'CCA
Cuts at: 0 503 1368
Size: 503 865
MslI CAynn'nnrTG
Cuts at: 0 142 334 482 1368
Size: 142 192 148 886
Fragments arranged by size:
886 192 148 142
MspA1I CmG'CkG
Cuts at: 0 67 128 235 773 890 1062 1368
Size: 67 61 107 538 117 172 306
Fragments arranged by size:
538 306 172 117 107 67 61
NarI GG'CG_CC
Cuts at: 0 829 1368
Size: 829 539
NspI r_CATG'y
Cuts at: 0 706 1368
Size: 706 662
PfoI T'CCnGG_A
Cuts at: 0 674 1368
Size: 674 694
PpuMI rG'GwC_Cy
Cuts at: 0 29 1035 1094 1368
Size: 29 1006 59 274
Fragments arranged by size:
1006 274 59 29
PspOMI G'GGCC_C
Cuts at: 0 80 1368
Size: 80 1288
PvuII CAG'CTG
Cuts at: 0 1062 1368
Size: 1062 306
SacI G_AGCT'C
Cuts at: 0 221 1368
Size: 221 1147
SacII CC_GC'GG
Cuts at: 0 68 1368
Size: 68 1300
SanDI GG'GwC_CC
Cuts at: 0 1035 1368
Size: 1035 333
SapI GCTCTTCn'nnn_
Cuts at: 0 657 1321 1368
Size: 657 664 47
Fragments arranged by size:
664 657 47
SfoI GGC'GCC
Cuts at: 0 830 1368
Size: 830 538
SmaI CCC'GGG
Cuts at: 0 73 1368
Size: 73 1295
SrfI GCCC'GGGC
Cuts at: 0 73 1368
Size: 73 1295
StyI C'CwwG_G
Cuts at: 0 498 1368
Size: 498 870
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 882 978 1368
Size: 706 176 96 390
Fragments arranged by size:
706 390 176 96
TatI w'GTAC_w
Cuts at: 0 1205 1368
Size: 1205 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 681 713 1368
Size: 359 32 290 32 655
Fragments arranged by size:
655 359 290 32 32
XmaI C'CCGG_G
Cuts at: 0 71 1368
Size: 71 1297
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BaeI BanI BanII BbeI BbsI BciVI
Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI
BsrFI BstAPI BstEII BstXI BtgI BtgZI BtsI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NspI PfoI PpuMI PspOMI PvuII SacI SacII SanDI
SapI SfoI SmaI SrfI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BamHI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I
ClaI CspCI DraIII DrdI EagI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI