Restriction map for
pF1KSDA0119
(Linear) (Six-base) MAPSORT of: KIAA0119.seq Check: 2042 from: 1 to: 969
KIAA0119.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
BanI G'GyrC_C
Cuts at: 0 64 969
Size: 64 905
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 969
Size: 236 733
BmrI ACTGGGnnnn_n'
Cuts at: 0 709 969
Size: 709 260
BmtI G_CTAG'C
Cuts at: 0 938 969
Size: 938 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 538 969
Size: 62 476 431
Fragments arranged by size:
476 431 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 969
Size: 529 440
BsaWI w'CCGG_w
Cuts at: 0 593 969
Size: 593 376
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 969
Size: 676 30 263
Fragments arranged by size:
676 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 969
Size: 187 782
BseYI C'CCAG_C
Cuts at: 0 130 753 969
Size: 130 623 216
Fragments arranged by size:
623 216 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 969
Size: 862 107
BsiHKAI G_wGCw'C
Cuts at: 0 654 704 969
Size: 654 50 265
Fragments arranged by size:
654 265 50
Bsp1286I G_dGCh'C
Cuts at: 0 654 704 969
Size: 654 50 265
Fragments arranged by size:
654 265 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 834 969
Size: 168 666 135
Fragments arranged by size:
666 168 135
BstYI r'GATC_y
Cuts at: 0 426 658 969
Size: 426 232 311
Fragments arranged by size:
426 311 232
BtgI C'CryG_G
Cuts at: 0 449 969
Size: 449 520
BtsI GCAGTG_nn'
Cuts at: 0 22 222 969
Size: 22 200 747
Fragments arranged by size:
747 200 22
EaeI y'GGCC_r
Cuts at: 0 36 188 476 803 969
Size: 36 152 288 327 166
Fragments arranged by size:
327 288 166 152 36
EarI CTCTTCn'nnn_
Cuts at: 0 217 969
Size: 217 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 969
Size: 127 842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 538 969
Size: 62 476 431
Fragments arranged by size:
476 431 62
EcoO109I rG'GnC_Cy
Cuts at: 0 711 969
Size: 711 258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 116 969
Size: 116 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 676 708 969
Size: 176 32 468 32 261
Fragments arranged by size:
468 261 176 32 32
HincII GTy'rAC
Cuts at: 0 333 863 969
Size: 333 530 106
Fragments arranged by size:
530 333 106
HindIII A'AGCT_T
Cuts at: 0 98 250 969
Size: 98 152 719
Fragments arranged by size:
719 152 98
MfeI C'AATT_G
Cuts at: 0 319 969
Size: 319 650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 309 969
Size: 162 147 660
Fragments arranged by size:
660 162 147
MscI TGG'CCA
Cuts at: 0 38 190 478 805 969
Size: 38 152 288 327 164
Fragments arranged by size:
327 288 164 152 38
MslI CAynn'nnrTG
Cuts at: 0 490 969
Size: 490 479
MspA1I CmG'CkG
Cuts at: 0 516 780 969
Size: 516 264 189
Fragments arranged by size:
516 264 189
NcoI C'CATG_G
Cuts at: 0 449 969
Size: 449 520
NheI G'CTAG_C
Cuts at: 0 934 969
Size: 934 35
PpuMI rG'GwC_Cy
Cuts at: 0 711 969
Size: 711 258
PstI C_TGCA'G
Cuts at: 0 785 870 969
Size: 785 85 99
Fragments arranged by size:
785 99 85
PvuII CAG'CTG
Cuts at: 0 516 780 969
Size: 516 264 189
Fragments arranged by size:
516 264 189
SexAI A'CCwGG_T
Cuts at: 0 566 969
Size: 566 403
SfcI C'TryA_G
Cuts at: 0 645 781 866 969
Size: 645 136 85 103
Fragments arranged by size:
645 136 103 85
SmlI C'TyrA_G
Cuts at: 0 544 969
Size: 544 425
SspI AAT'ATT
Cuts at: 0 413 969
Size: 413 556
StyI C'CwwG_G
Cuts at: 0 293 449 728 969
Size: 293 156 279 241
Fragments arranged by size:
293 279 241 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 969
Size: 669 300
TatI w'GTAC_w
Cuts at: 0 548 969
Size: 548 421
Tth111I GACn'n_nGTC
Cuts at: 0 715 969
Size: 715 254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 784 969
Size: 784 185
Enzymes that do cut:
BanI BbsI BmrI BmtI BpmI BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstYI BtgI
BtsI EaeI EarI EciI Eco57MI EcoO109I FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI
NheI PpuMI PstI PvuII SexAI SfcI SmlI SspI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TstI XbaI XhoI XmaI XmnI ZraI