Restriction map for
pF1KSDA0122
(Linear) (Six-base) MAPSORT of: KIAA0122.seq Check: 6965 from: 1 to: 2787
KIAA0122.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 1703 2787
Size: 1703 1084
AccI GT'mk_AC
Cuts at: 0 1295 1576 2534 2787
Size: 1295 281 958 253
Fragments arranged by size:
1295 958 281 253
Acc65I G'GTAC_C
Cuts at: 0 1264 2787
Size: 1264 1523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 2787
Size: 482 2305
AhdI GACnn_n'nnGTC
Cuts at: 0 1467 2787
Size: 1467 1320
AleI CACnn'nnGTG
Cuts at: 0 617 2787
Size: 617 2170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2605 2787
Size: 2605 182
AlwNI CAG_nnn'CTG
Cuts at: 0 421 738 771 804 1074 1083 1092 1812
Size: 421 317 33 33 270 9 9 720
Cuts at: 1812 2062 2071 2271 2530 2787
Size: 250 9 200 259 257
Fragments arranged by size:
720 421 317 270 259 257 250 200
33 33 9 9 9
ApaI G_GGCC'C
Cuts at: 0 957 1393 1974 2236 2787
Size: 957 436 581 262 551
Fragments arranged by size:
957 581 551 436 262
ApaLI G'TGCA_C
Cuts at: 0 2696 2787
Size: 2696 91
AvaI C'yCGr_G
Cuts at: 0 219 290 1435 2086 2479 2552 2787
Size: 219 71 1145 651 393 73 235
Fragments arranged by size:
1145 651 393 235 219 73 71
BamHI G'GATC_C
Cuts at: 0 1416 2787
Size: 1416 1371
BanI G'GyrC_C
Cuts at: 0 960 1264 1381 1887 2324 2787
Size: 960 304 117 506 437 463
Fragments arranged by size:
960 506 463 437 304 117
BanII G_rGCy'C
Cuts at: 0 226 871 957 1393 1613 1621 1974 2236
Size: 226 645 86 436 220 8 353 262
Cuts at: 2236 2345 2379 2495 2686 2787
Size: 109 34 116 191 101
Fragments arranged by size:
645 436 353 262 226 220 191 116
109 101 86 34 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1343 2787
Size: 1343 1444
BbvCI CC'TCA_GC
Cuts at: 0 397 2787
Size: 397 2390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 677 711 2533 2567 2787
Size: 677 34 1822 34 220
Fragments arranged by size:
1822 677 220 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 155 272 281 1201 1231 2787
Size: 155 117 9 920 30 1556
Fragments arranged by size:
1556 920 155 117 30 9
BlpI GC'TnA_GC
Cuts at: 0 793 2787
Size: 793 1994
Bme1580I G_kGCm'C
Cuts at: 0 716 957 1345 1384 1393 1555 1890 1974
Size: 716 241 388 39 9 162 335 84
Cuts at: 1974 2236 2700 2787
Size: 262 464 87
Fragments arranged by size:
716 464 388 335 262 241 162 87
84 39 9
BmgBI CAC'GTC
Cuts at: 0 16 2787
Size: 16 2771
BmrI ACTGGGnnnn_n'
Cuts at: 0 787 1221 1773 1827 2787
Size: 787 434 552 54 960
Fragments arranged by size:
960 787 552 434 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 897 2787
Size: 897 1890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1445 1597 1873 2787
Size: 1445 152 276 914
Fragments arranged by size:
1445 914 276 152
Bpu10I CC'TnA_GC
Cuts at: 0 397 2237 2787
Size: 397 1840 550
Fragments arranged by size:
1840 550 397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 2380 2787
Size: 1360 1020 407
Fragments arranged by size:
1360 1020 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 89 1717 2787
Size: 89 1628 1070
Fragments arranged by size:
1628 1070 89
BsaHI Gr'CG_yC
Cuts at: 0 668 1700 2292 2787
Size: 668 1032 592 495
Fragments arranged by size:
1032 668 592 495
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 1150 1180 1759 1789 2787
Size: 199 30 921 30 579 30 998
Fragments arranged by size:
998 921 579 199 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 352 355 358 361 364 367 376
Size: 199 153 3 3 3 3 3 9
Cuts at: 376 379 382 385 972 1297 2227 2230 2260
Size: 3 3 3 587 325 930 3 30
Cuts at: 2260 2514 2787
Size: 254 273
Fragments arranged by size:
930 587 325 273 254 199 153 30
9 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 1067 1493 1967 2302 2562 2787
Size: 1067 426 474 335 260 225
Fragments arranged by size:
1067 474 426 335 260 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 2468 2734 2787
Size: 1078 1390 266 53
Fragments arranged by size:
1390 1078 266 53
BsiEI CG_ry'CG
Cuts at: 0 1295 1865 2157 2787
Size: 1295 570 292 630
Fragments arranged by size:
1295 630 570 292
BsiHKAI G_wGCw'C
Cuts at: 0 1601 1613 2103 2700 2787
Size: 1601 12 490 597 87
Fragments arranged by size:
1601 597 490 87 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 287 1707 2787
Size: 287 1420 1080
Fragments arranged by size:
1420 1080 287
Bsp1286I G_dGCh'C
Cuts at: 0 226 716 871 957 1345 1384 1393 1555
Size: 226 490 155 86 388 39 9 162
Cuts at: 1555 1601 1613 1621 1890 1974 2103 2236 2345
Size: 46 12 8 269 84 129 133 109
Cuts at: 2345 2379 2495 2686 2700 2787
Size: 34 116 191 14 87
Fragments arranged by size:
490 388 269 226 191 162 155 133
129 116 109 87 86 84 46 39
34 14 12 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1420 2787
Size: 1420 1367
BsrBI CCG'CTC
Cuts at: 0 62 2241 2787
Size: 62 2179 546
Fragments arranged by size:
2179 546 62
BsrDI GCAATG_nn'
Cuts at: 0 1214 2787
Size: 1214 1573
BsrFI r'CCGG_y
Cuts at: 0 271 1290 1647 1753 2369 2787
Size: 271 1019 357 106 616 418
Fragments arranged by size:
1019 616 418 357 271 106
BssSI C'ACGA_G
Cuts at: 0 750 2787
Size: 750 2037
BstAPI GCAn_nnn'nTGC
Cuts at: 0 459 738 771 1083 1092 2787
Size: 459 279 33 312 9 1695
Fragments arranged by size:
1695 459 312 279 33 9
BstEII G'GTnAC_C
Cuts at: 0 2718 2763 2787
Size: 2718 45 24
Fragments arranged by size:
2718 45 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1959 2787
Size: 1959 828
BstYI r'GATC_y
Cuts at: 0 1079 1416 2130 2787
Size: 1079 337 714 657
Fragments arranged by size:
1079 714 657 337
BstZ17I GTA'TAC
Cuts at: 0 1577 2787
Size: 1577 1210
BtgI C'CryG_G
Cuts at: 0 435 935 1026 1365 2600 2787
Size: 435 500 91 339 1235 187
Fragments arranged by size:
1235 500 435 339 187 91
BtsI GCAGTG_nn'
Cuts at: 0 769 1208 1553 1661 2060 2574 2746 2787
Size: 769 439 345 108 399 514 172 41
Fragments arranged by size:
769 514 439 399 345 172 108 41
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 645 680 2787
Size: 645 35 2107
Fragments arranged by size:
2107 645 35
DraIII CAC_nnn'GTG
Cuts at: 0 459 781 2787
Size: 459 322 2006
Fragments arranged by size:
2006 459 322
DrdI GACnn_nn'nnGTC
Cuts at: 0 20 2787
Size: 20 2767
EaeI y'GGCC_r
Cuts at: 0 39 438 783 1202 1862 2144 2787
Size: 39 399 345 419 660 282 643
Fragments arranged by size:
660 643 419 399 345 282 39
EagI C'GGCC_G
Cuts at: 0 1862 2787
Size: 1862 925
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1848 2787
Size: 1848 939
EcoICRI GAG'CTC
Cuts at: 0 1611 2787
Size: 1611 1176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1445 1597 1873 2787
Size: 482 963 152 276 914
Fragments arranged by size:
963 914 482 276 152
EcoNI CCTnn'n_nnAGG
Cuts at: 0 862 2171 2787
Size: 862 1309 616
Fragments arranged by size:
1309 862 616
EcoO109I rG'GnC_Cy
Cuts at: 0 801 953 954 1089 1389 1390 1432 2232
Size: 801 152 1 135 300 1 42 800
Cuts at: 2232 2233 2787
Size: 1 554
Fragments arranged by size:
801 800 554 300 152 135 42 1
1 1
FspI TGC'GCA
Cuts at: 0 913 2787
Size: 913 1874
HaeII r_GCGC'y
Cuts at: 0 2317 2787
Size: 2317 470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 744 776 1150 1182 1453 1485
Size: 163 32 549 32 374 32 271 32
Cuts at: 1485 1711 1743 1759 1791 2787
Size: 226 32 16 32 996
Fragments arranged by size:
996 549 374 271 226 163 32 32
32 32 32 32 16
HincII GTy'rAC
Cuts at: 0 1296 2787
Size: 1296 1491
KpnI G_GTAC'C
Cuts at: 0 1268 2787
Size: 1268 1519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 2566 2787
Size: 1010 1556 221
Fragments arranged by size:
1556 1010 221
MscI TGG'CCA
Cuts at: 0 440 1204 2146 2787
Size: 440 764 942 641
Fragments arranged by size:
942 764 641 440
MslI CAynn'nnrTG
Cuts at: 0 167 391 617 2125 2787
Size: 167 224 226 1508 662
Fragments arranged by size:
1508 662 226 224 167
MspA1I CmG'CkG
Cuts at: 0 444 1028 1071 1594 2195 2452 2621 2787
Size: 444 584 43 523 601 257 169 166
Fragments arranged by size:
601 584 523 444 257 169 166 43
NcoI C'CATG_G
Cuts at: 0 935 1365 2600 2787
Size: 935 430 1235 187
Fragments arranged by size:
1235 935 430 187
NruI TCG'CGA
Cuts at: 0 137 2787
Size: 137 2650
PasI CC'CwG_GG
Cuts at: 0 834 2787
Size: 834 1953
PflMI CCAn_nnn'nTGG
Cuts at: 0 781 2606 2787
Size: 781 1825 181
Fragments arranged by size:
1825 781 181
PpuMI rG'GwC_Cy
Cuts at: 0 1432 2787
Size: 1432 1355
PspOMI G'GGCC_C
Cuts at: 0 953 1389 1970 2232 2787
Size: 953 436 581 262 555
Fragments arranged by size:
953 581 555 436 262
PstI C_TGCA'G
Cuts at: 0 449 1079 1088 2067 2597 2787
Size: 449 630 9 979 530 190
Fragments arranged by size:
979 630 530 449 190 9
PvuII CAG'CTG
Cuts at: 0 444 1071 2452 2787
Size: 444 627 1381 335
Fragments arranged by size:
1381 627 444 335
RsrII CG'GwC_CG
Cuts at: 0 323 2787
Size: 323 2464
SacI G_AGCT'C
Cuts at: 0 1613 2787
Size: 1613 1174
SacII CC_GC'GG
Cuts at: 0 1029 2787
Size: 1029 1758
SalI G'TCGA_C
Cuts at: 0 1294 2787
Size: 1294 1493
SbfI CC_TGCA'GG
Cuts at: 0 1088 2787
Size: 1088 1699
SfcI C'TryA_G
Cuts at: 0 445 615 1075 1084 1299 1677 2063 2190
Size: 445 170 460 9 215 378 386 127
Cuts at: 2190 2531 2593 2787
Size: 341 62 194
Fragments arranged by size:
460 445 386 378 341 215 194 170
127 62 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1231 2787
Size: 1231 1556
SgrAI Cr'CCGG_yG
Cuts at: 0 2369 2787
Size: 2369 418
SmaI CCC'GGG
Cuts at: 0 221 2787
Size: 221 2566
SmlI C'TyrA_G
Cuts at: 0 1375 2086 2359 2787
Size: 1375 711 273 428
Fragments arranged by size:
1375 711 428 273
StuI AGG'CCT
Cuts at: 0 214 1246 1441 2787
Size: 214 1032 195 1346
Fragments arranged by size:
1346 1032 214 195
StyI C'CwwG_G
Cuts at: 0 935 1142 1251 1365 1385 1952 2600 2787
Size: 935 207 109 114 20 567 648 187
Fragments arranged by size:
935 648 567 207 187 114 109 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 769 1112 2683 2787
Size: 340 429 343 1571 104
Fragments arranged by size:
1571 429 343 340 104
TatI w'GTAC_w
Cuts at: 0 1814 2787
Size: 1814 973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 2787
Size: 637 32 2118
Fragments arranged by size:
2118 637 32
XhoI C'TCGA_G
Cuts at: 0 2086 2787
Size: 2086 701
XmaI C'CCGG_G
Cuts at: 0 219 2787
Size: 219 2568
XmnI GAAnn'nnTTC
Cuts at: 0 697 2787
Size: 697 2090
ZraI GAC'GTC
Cuts at: 0 1701 2787
Size: 1701 1086
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtsI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KpnI MmeI
MscI MslI MspA1I NcoI NruI PasI PflMI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SalI SbfI
SfcI SfiI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI
BfrBI BglII BmtI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstBI Bsu36I BtgZI ClaI
DraI EarI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI SanDI
SapI ScaI SexAI SfoI SgfI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI