Restriction map for
pF1KA0128
(Linear) (Six-base) MAPSORT of: KIAA0128.seq Check: 1190 from: 1 to: 1281
KIAA0128.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 704 1281
Size: 704 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 751 936 1132 1276 1281
Size: 505 246 185 196 144 5
Fragments arranged by size:
505 246 196 185 144 5
AflIII A'CryG_T
Cuts at: 0 63 1281
Size: 63 1218
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 1281
Size: 169 32 1080
Fragments arranged by size:
1080 169 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1254 1281
Size: 1254 27
ApoI r'AATT_y
Cuts at: 0 196 358 1281
Size: 196 162 923
Fragments arranged by size:
923 196 162
AvrII C'CTAG_G
Cuts at: 0 993 1281
Size: 993 288
BanII G_rGCy'C
Cuts at: 0 1070 1231 1281
Size: 1070 161 50
Fragments arranged by size:
1070 161 50
BciVI GTATCCnnnnn_n'
Cuts at: 0 776 1281
Size: 776 505
BglII A'GATC_T
Cuts at: 0 632 1281
Size: 632 649
BlpI GC'TnA_GC
Cuts at: 0 1208 1281
Size: 1208 73
Bme1580I G_kGCm'C
Cuts at: 0 778 1281
Size: 778 503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 928 988 1165 1201 1259 1281
Size: 226 702 60 177 36 58 22
Fragments arranged by size:
702 226 177 60 58 36 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 1281
Size: 418 30 833
Fragments arranged by size:
833 418 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 925 1033 1281
Size: 925 108 248
Fragments arranged by size:
925 248 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1075 1105 1281
Size: 802 273 30 176
Fragments arranged by size:
802 273 176 30
BsiEI CG_ry'CG
Cuts at: 0 11 296 1281
Size: 11 285 985
Fragments arranged by size:
985 285 11
BsiHKAI G_wGCw'C
Cuts at: 0 1070 1281
Size: 1070 211
BsmI GAATG_Cn'
Cuts at: 0 1185 1281
Size: 1185 96
Bsp1286I G_dGCh'C
Cuts at: 0 778 1070 1231 1281
Size: 778 292 161 50
Fragments arranged by size:
778 292 161 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 704 774 1281
Size: 395 309 70 507
Fragments arranged by size:
507 395 309 70
BsrDI GCAATG_nn'
Cuts at: 0 461 545 1281
Size: 461 84 736
Fragments arranged by size:
736 461 84
BssSI C'ACGA_G
Cuts at: 0 1093 1281
Size: 1093 188
BstBI TT'CG_AA
Cuts at: 0 200 568 1281
Size: 200 368 713
Fragments arranged by size:
713 368 200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 1281
Size: 174 1107
BstYI r'GATC_y
Cuts at: 0 404 632 852 1281
Size: 404 228 220 429
Fragments arranged by size:
429 404 228 220
BtgI C'CryG_G
Cuts at: 0 470 1281
Size: 470 811
BtsI GCAGTG_nn'
Cuts at: 0 801 1281
Size: 801 480
ClaI AT'CG_AT
Cuts at: 0 365 1281
Size: 365 916
EarI CTCTTCn'nnn_
Cuts at: 0 389 405 565 1010 1252 1281
Size: 389 16 160 445 242 29
Fragments arranged by size:
445 389 242 160 29 16
EcoICRI GAG'CTC
Cuts at: 0 1068 1281
Size: 1068 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 505 751 928 936 988 1132 1165
Size: 226 279 246 177 8 52 144 33
Cuts at: 1165 1201 1259 1276 1281
Size: 36 58 17 5
Fragments arranged by size:
279 246 226 177 144 58 52 36
33 17 8 5
EcoRI G'AATT_C
Cuts at: 0 358 1281
Size: 358 923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 617 643 649 675 1281
Size: 617 26 6 26 606
Fragments arranged by size:
617 606 26 26 6
HincII GTy'rAC
Cuts at: 0 843 1281
Size: 843 438
MslI CAynn'nnrTG
Cuts at: 0 172 1281
Size: 172 1109
MspA1I CmG'CkG
Cuts at: 0 93 899 1281
Size: 93 806 382
Fragments arranged by size:
806 382 93
NdeI CA'TA_TG
Cuts at: 0 969 1281
Size: 969 312
NspI r_CATG'y
Cuts at: 0 67 1281
Size: 67 1214
PasI CC'CwG_GG
Cuts at: 0 1224 1281
Size: 1224 57
PciI A'CATG_T
Cuts at: 0 63 1281
Size: 63 1218
PstI C_TGCA'G
Cuts at: 0 392 1281
Size: 392 889
PvuI CG_AT'CG
Cuts at: 0 296 1281
Size: 296 985
PvuII CAG'CTG
Cuts at: 0 93 1281
Size: 93 1188
SacI G_AGCT'C
Cuts at: 0 1070 1281
Size: 1070 211
SapI GCTCTTCn'nnn_
Cuts at: 0 389 1281
Size: 389 892
SbfI CC_TGCA'GG
Cuts at: 0 392 1281
Size: 392 889
SfcI C'TryA_G
Cuts at: 0 388 647 1281
Size: 388 259 634
Fragments arranged by size:
634 388 259
StuI AGG'CCT
Cuts at: 0 382 1281
Size: 382 899
StyI C'CwwG_G
Cuts at: 0 769 993 1281
Size: 769 224 288
Fragments arranged by size:
769 288 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 25 232 1281
Size: 19 6 207 1049
Fragments arranged by size:
1049 207 19 6
XmnI GAAnn'nnTTC
Cuts at: 0 988 1183 1281
Size: 988 195 98
Fragments arranged by size:
988 195 98
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApoI AvrII BanII
BciVI BglII BlpI Bme1580I BpmI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstBI
BstXI BstYI BtgI BtsI ClaI EarI EcoICRI Eco57MI
EcoRI Hin4I HincII MslI MspA1I NdeI NspI PasI
PciI PstI PvuI PvuII SacI SapI SbfI SfcI
StuI StyI TaqII XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI RsrII SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI