Restriction map for
pF1KA0129
(Linear) (Six-base) MAPSORT of: KIAA0129.seq Check: 3923 from: 1 to: 1326
KIAA0129.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1247 1326
Size: 1247 79
Acc65I G'GTAC_C
Cuts at: 0 742 1326
Size: 742 584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 517 1326
Size: 352 165 809
Fragments arranged by size:
809 352 165
AfeI AGC'GCT
Cuts at: 0 380 1096 1326
Size: 380 716 230
Fragments arranged by size:
716 380 230
AflII C'TTAA_G
Cuts at: 0 691 1326
Size: 691 635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1159 1326
Size: 1159 167
AlwNI CAG_nnn'CTG
Cuts at: 0 494 1326
Size: 494 832
ApaI G_GGCC'C
Cuts at: 0 896 1326
Size: 896 430
ApaLI G'TGCA_C
Cuts at: 0 196 1326
Size: 196 1130
ApoI r'AATT_y
Cuts at: 0 340 391 1326
Size: 340 51 935
Fragments arranged by size:
935 340 51
AvaI C'yCGr_G
Cuts at: 0 580 1326
Size: 580 746
BamHI G'GATC_C
Cuts at: 0 813 1326
Size: 813 513
BanI G'GyrC_C
Cuts at: 0 742 994 1034 1085 1326
Size: 742 252 40 51 241
Fragments arranged by size:
742 252 241 51 40
BanII G_rGCy'C
Cuts at: 0 101 515 620 896 1326
Size: 101 414 105 276 430
Fragments arranged by size:
430 414 276 105 101
BbeI G_GCGC'C
Cuts at: 0 998 1038 1326
Size: 998 40 288
Fragments arranged by size:
998 288 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 1170 1326
Size: 1170 156
BglI GCCn_nnn'nGGC
Cuts at: 0 881 1326
Size: 881 445
BlpI GC'TnA_GC
Cuts at: 0 95 1326
Size: 95 1231
Bme1580I G_kGCm'C
Cuts at: 0 200 605 896 902 1326
Size: 200 405 291 6 424
Fragments arranged by size:
424 405 291 200 6
BmgBI CAC'GTC
Cuts at: 0 981 1218 1326
Size: 981 237 108
Fragments arranged by size:
981 237 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 1122 1326
Size: 978 144 204
Fragments arranged by size:
978 204 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 406 516 1326
Size: 205 201 110 810
Fragments arranged by size:
810 205 201 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1075 1126 1326
Size: 283 792 51 200
Fragments arranged by size:
792 283 200 51
BsaAI yAC'GTr
Cuts at: 0 201 1326
Size: 201 1125
BsaHI Gr'CG_yC
Cuts at: 0 673 995 1035 1175 1199 1326
Size: 673 322 40 140 24 127
Fragments arranged by size:
673 322 140 127 40 24
BseYI C'CCAG_C
Cuts at: 0 147 879 1326
Size: 147 732 447
Fragments arranged by size:
732 447 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1003 1326
Size: 223 780 323
Fragments arranged by size:
780 323 223
BsiEI CG_ry'CG
Cuts at: 0 17 87 855 960 1052 1167 1326
Size: 17 70 768 105 92 115 159
Fragments arranged by size:
768 159 115 105 92 70 17
BsiHKAI G_wGCw'C
Cuts at: 0 101 200 1326
Size: 101 99 1126
Fragments arranged by size:
1126 101 99
Bsp1286I G_dGCh'C
Cuts at: 0 101 200 515 605 620 896 902 1326
Size: 101 99 315 90 15 276 6 424
Fragments arranged by size:
424 315 276 101 99 90 15 6
BspHI T'CATG_A
Cuts at: 0 470 1326
Size: 470 856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1247 1326
Size: 1247 79
BsrBI CCG'CTC
Cuts at: 0 1272 1326
Size: 1272 54
BsrFI r'CCGG_y
Cuts at: 0 955 997 1166 1195 1326
Size: 955 42 169 29 131
Fragments arranged by size:
955 169 131 42 29
BsrGI T'GTAC_A
Cuts at: 0 428 1326
Size: 428 898
BssHII G'CGCG_C
Cuts at: 0 131 152 796 832 1326
Size: 131 21 644 36 494
Fragments arranged by size:
644 494 131 36 21
BssSI C'ACGA_G
Cuts at: 0 643 938 1326
Size: 643 295 388
Fragments arranged by size:
643 388 295
BstAPI GCAn_nnn'nTGC
Cuts at: 0 561 707 1326
Size: 561 146 619
Fragments arranged by size:
619 561 146
BstYI r'GATC_y
Cuts at: 0 813 1326
Size: 813 513
BtgI C'CryG_G
Cuts at: 0 159 653 1038 1326
Size: 159 494 385 288
Fragments arranged by size:
494 385 288 159
DraI TTT'AAA
Cuts at: 0 227 1326
Size: 227 1099
DraIII CAC_nnn'GTG
Cuts at: 0 171 1326
Size: 171 1155
EaeI y'GGCC_r
Cuts at: 0 162 383 545 957 1049 1128 1326
Size: 162 221 162 412 92 79 198
Fragments arranged by size:
412 221 198 162 162 92 79
EagI C'GGCC_G
Cuts at: 0 957 1049 1326
Size: 957 92 277
Fragments arranged by size:
957 277 92
EarI CTCTTCn'nnn_
Cuts at: 0 106 365 1326
Size: 106 259 961
Fragments arranged by size:
961 259 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 837 1326
Size: 837 489
EcoICRI GAG'CTC
Cuts at: 0 99 1326
Size: 99 1227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 352 406 516 517 1326
Size: 205 147 54 110 1 809
Fragments arranged by size:
809 205 147 110 54 1
EcoO109I rG'GnC_Cy
Cuts at: 0 639 892 1043 1326
Size: 639 253 151 283
Fragments arranged by size:
639 283 253 151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 648 1326
Size: 648 678
HaeII r_GCGC'y
Cuts at: 0 69 184 382 998 1038 1098 1326
Size: 69 115 198 616 40 60 228
Fragments arranged by size:
616 228 198 115 69 60 40
HincII GTy'rAC
Cuts at: 0 978 1326
Size: 978 348
KasI G'GCGC_C
Cuts at: 0 994 1034 1326
Size: 994 40 292
Fragments arranged by size:
994 292 40
KpnI G_GTAC'C
Cuts at: 0 746 1326
Size: 746 580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 509 1326
Size: 20 489 817
Fragments arranged by size:
817 489 20
MscI TGG'CCA
Cuts at: 0 164 385 1326
Size: 164 221 941
Fragments arranged by size:
941 221 164
MspA1I CmG'CkG
Cuts at: 0 113 122 193 234 1040 1326
Size: 113 9 71 41 806 286
Fragments arranged by size:
806 286 113 71 41 9
NaeI GCC'GGC
Cuts at: 0 999 1197 1326
Size: 999 198 129
Fragments arranged by size:
999 198 129
NarI GG'CG_CC
Cuts at: 0 995 1035 1326
Size: 995 40 291
Fragments arranged by size:
995 291 40
NgoMIV G'CCGG_C
Cuts at: 0 997 1195 1326
Size: 997 198 131
Fragments arranged by size:
997 198 131
PasI CC'CwG_GG
Cuts at: 0 31 1326
Size: 31 1295
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 1326
Size: 171 1155
PfoI T'CCnGG_A
Cuts at: 0 1181 1262 1326
Size: 1181 81 64
Fragments arranged by size:
1181 81 64
PmlI CAC'GTG
Cuts at: 0 201 1326
Size: 201 1125
PspOMI G'GGCC_C
Cuts at: 0 892 1326
Size: 892 434
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 341 373 1326
Size: 341 32 953
Fragments arranged by size:
953 341 32
PvuI CG_AT'CG
Cuts at: 0 855 1326
Size: 855 471
PvuII CAG'CTG
Cuts at: 0 234 1326
Size: 234 1092
SacI G_AGCT'C
Cuts at: 0 101 1326
Size: 101 1225
SacII CC_GC'GG
Cuts at: 0 1041 1326
Size: 1041 285
SapI GCTCTTCn'nnn_
Cuts at: 0 106 1326
Size: 106 1220
ScaI AGT'ACT
Cuts at: 0 1111 1326
Size: 1111 215
SfoI GGC'GCC
Cuts at: 0 996 1036 1326
Size: 996 40 290
Fragments arranged by size:
996 290 40
SmlI C'TyrA_G
Cuts at: 0 298 691 1090 1105 1326
Size: 298 393 399 15 221
Fragments arranged by size:
399 393 298 221 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 996 1326
Size: 996 330
TatI w'GTAC_w
Cuts at: 0 428 661 1109 1326
Size: 428 233 448 217
Fragments arranged by size:
448 428 233 217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 798 830 1326
Size: 798 32 496
Fragments arranged by size:
798 496 32
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI
BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstYI BtgI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII HincII KasI KpnI MmeI MscI
MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PmlI
PspOMI PsrI PvuI PvuII SacI SacII SapI ScaI
SfoI SmlI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI AclI AflIII AgeI AhdI AleI AloI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BclI BfrBI BglII BmtI BplI Bpu10I BsaI BsaBI
BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BsrDI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HindIII HpaI MfeI MluI MslI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI RsrII SalI
SanDI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI