Restriction map for
pF1KA0130
(Linear) (Six-base) MAPSORT of: KIAA0130.seq Check: 1748 from: 1 to: 2967
KIAA0130.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 2171 2222 2967
Size: 2171 51 745
Fragments arranged by size:
2171 745 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 211 469 1045 2278 2967
Size: 37 174 258 576 1233 689
Fragments arranged by size:
1233 689 576 258 174 37
AfeI AGC'GCT
Cuts at: 0 49 1060 2967
Size: 49 1011 1907
Fragments arranged by size:
1907 1011 49
AflIII A'CryG_T
Cuts at: 0 305 2967
Size: 305 2662
AleI CACnn'nnGTG
Cuts at: 0 1657 2071 2851 2967
Size: 1657 414 780 116
Fragments arranged by size:
1657 780 414 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 363 2845 2967
Size: 363 2482 122
Fragments arranged by size:
2482 363 122
AlwNI CAG_nnn'CTG
Cuts at: 0 412 457 492 786 1558 2967
Size: 412 45 35 294 772 1409
Fragments arranged by size:
1409 772 412 294 45 35
ApaI G_GGCC'C
Cuts at: 0 528 1470 2967
Size: 528 942 1497
Fragments arranged by size:
1497 942 528
ApoI r'AATT_y
Cuts at: 0 85 1399 1417 2967
Size: 85 1314 18 1550
Fragments arranged by size:
1550 1314 85 18
AvaI C'yCGr_G
Cuts at: 0 266 748 889 2412 2967
Size: 266 482 141 1523 555
Fragments arranged by size:
1523 555 482 266 141
BanI G'GyrC_C
Cuts at: 0 655 754 1563 2967
Size: 655 99 809 1404
Fragments arranged by size:
1404 809 655 99
BanII G_rGCy'C
Cuts at: 0 369 528 878 908 1244 1470 2587 2620
Size: 369 159 350 30 336 226 1117 33
Cuts at: 2620 2967
Size: 347
Fragments arranged by size:
1117 369 350 347 336 226 159 33
30
BbsI GAAGACnn'nnnn_
Cuts at: 0 2519 2967
Size: 2519 448
BbvCI CC'TCA_GC
Cuts at: 0 505 1559 2917 2967
Size: 505 1054 1358 50
Fragments arranged by size:
1358 1054 505 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2553 2587 2967
Size: 2553 34 380
Fragments arranged by size:
2553 380 34
BclI T'GATC_A
Cuts at: 0 1991 2967
Size: 1991 976
BfrBI ATG'CAT
Cuts at: 0 350 2967
Size: 350 2617
BglI GCCn_nnn'nGGC
Cuts at: 0 2434 2967
Size: 2434 533
BglII A'GATC_T
Cuts at: 0 851 2967
Size: 851 2116
BlpI GC'TnA_GC
Cuts at: 0 1148 2581 2967
Size: 1148 1433 386
Fragments arranged by size:
1433 1148 386
Bme1580I G_kGCm'C
Cuts at: 0 528 757 1470 1568 2419 2451 2967
Size: 528 229 713 98 851 32 516
Fragments arranged by size:
851 713 528 516 229 98 32
BmgBI CAC'GTC
Cuts at: 0 1881 2028 2967
Size: 1881 147 939
Fragments arranged by size:
1881 939 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 2757 2967
Size: 2757 210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 2967
Size: 573 2394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 434 457 514 868 2029 2176 2824
Size: 72 362 23 57 354 1161 147 648
Cuts at: 2824 2967
Size: 143
Fragments arranged by size:
1161 648 362 354 147 143 72 57
23
Bpu10I CC'TnA_GC
Cuts at: 0 505 1559 2917 2967
Size: 505 1054 1358 50
Fragments arranged by size:
1358 1054 505 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 916 1246 2967
Size: 892 24 330 1721
Fragments arranged by size:
1721 892 330 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1570 2744 2967
Size: 1570 1174 223
Fragments arranged by size:
1570 1174 223
BsaHI Gr'CG_yC
Cuts at: 0 2219 2482 2967
Size: 2219 263 485
Fragments arranged by size:
2219 485 263
BsaWI w'CCGG_w
Cuts at: 0 632 1293 1883 2967
Size: 632 661 590 1084
Fragments arranged by size:
1084 661 632 590
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1547 1577 2002 2032 2164 2194
Size: 895 30 622 30 425 30 132 30
Cuts at: 2194 2967
Size: 773
Fragments arranged by size:
895 773 622 425 132 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 916 1618 1680 2373 2454 2715 2800
Size: 565 351 702 62 693 81 261 85
Cuts at: 2800 2967
Size: 167
Fragments arranged by size:
702 693 565 351 261 167 85 81
62
BseYI C'CCAG_C
Cuts at: 0 457 1178 1305 1890 2165 2349 2967
Size: 457 721 127 585 275 184 618
Fragments arranged by size:
721 618 585 457 275 184 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 517 1729 2191 2575 2791 2945 2967
Size: 384 133 1212 462 384 216 154 22
Fragments arranged by size:
1212 462 384 384 216 154 133 22
BsiHKAI G_wGCw'C
Cuts at: 0 908 1172 2279 2587 2967
Size: 908 264 1107 308 380
Fragments arranged by size:
1107 908 380 308 264
BsmI GAATG_Cn'
Cuts at: 0 352 579 1203 2967
Size: 352 227 624 1764
Fragments arranged by size:
1764 624 352 227
BsmBI CGTCTCn'nnnn_
Cuts at: 0 769 2257 2967
Size: 769 1488 710
Fragments arranged by size:
1488 769 710
Bsp1286I G_dGCh'C
Cuts at: 0 369 528 757 878 908 1172 1244 1470
Size: 369 159 229 121 30 264 72 226
Cuts at: 1470 1568 2279 2419 2451 2587 2620 2967
Size: 98 711 140 32 136 33 347
Fragments arranged by size:
711 369 347 264 229 226 159 140
136 121 98 72 33 32 30
BspEI T'CCGG_A
Cuts at: 0 632 1883 2967
Size: 632 1251 1084
Fragments arranged by size:
1251 1084 632
BspHI T'CATG_A
Cuts at: 0 1994 2967
Size: 1994 973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1600 2105 2967
Size: 1600 505 862
Fragments arranged by size:
1600 862 505
BsrBI CCG'CTC
Cuts at: 0 420 2180 2967
Size: 420 1760 787
Fragments arranged by size:
1760 787 420
BsrDI GCAATG_nn'
Cuts at: 0 286 2954 2967
Size: 286 2668 13
Fragments arranged by size:
2668 286 13
BsrFI r'CCGG_y
Cuts at: 0 442 715 1452 2967
Size: 442 273 737 1515
Fragments arranged by size:
1515 737 442 273
BsrGI T'GTAC_A
Cuts at: 0 1075 2967
Size: 1075 1892
BstAPI GCAn_nnn'nTGC
Cuts at: 0 412 2283 2838 2967
Size: 412 1871 555 129
Fragments arranged by size:
1871 555 412 129
BstBI TT'CG_AA
Cuts at: 0 2563 2967
Size: 2563 404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1099 1519 1659 2073 2154 2967
Size: 1099 420 140 414 81 813
Fragments arranged by size:
1099 813 420 414 140 81
BstYI r'GATC_y
Cuts at: 0 602 851 1613 2967
Size: 602 249 762 1354
Fragments arranged by size:
1354 762 602 249
Bsu36I CC'TnA_GG
Cuts at: 0 1601 2967
Size: 1601 1366
BtgI C'CryG_G
Cuts at: 0 2817 2967
Size: 2817 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 522 2967
Size: 522 2445
BtsI GCAGTG_nn'
Cuts at: 0 655 2297 2417 2779 2967
Size: 655 1642 120 362 188
Fragments arranged by size:
1642 655 362 188 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 929 964 2967
Size: 929 35 2003
Fragments arranged by size:
2003 929 35
DraIII CAC_nnn'GTG
Cuts at: 0 738 2967
Size: 738 2229
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 2967
Size: 277 2690
EaeI y'GGCC_r
Cuts at: 0 2435 2702 2814 2903 2967
Size: 2435 267 112 89 64
Fragments arranged by size:
2435 267 112 89 64
EarI CTCTTCn'nnn_
Cuts at: 0 231 564 1330 2494 2542 2967
Size: 231 333 766 1164 48 425
Fragments arranged by size:
1164 766 425 333 231 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1252 1630 1918 2967
Size: 1252 378 288 1049
Fragments arranged by size:
1252 1049 378 288
EcoICRI GAG'CTC
Cuts at: 0 906 2585 2967
Size: 906 1679 382
Fragments arranged by size:
1679 906 382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 72 211 434 457 469 514 868
Size: 37 35 139 223 23 12 45 354
Cuts at: 868 1045 2029 2176 2278 2824 2967
Size: 177 984 147 102 546 143
Fragments arranged by size:
984 546 354 223 177 147 143 139
102 45 37 35 23 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 748 2245 2806 2967
Size: 748 1497 561 161
Fragments arranged by size:
1497 748 561 161
EcoO109I rG'GnC_Cy
Cuts at: 0 270 525 1467 1785 2682 2967
Size: 270 255 942 318 897 285
Fragments arranged by size:
942 897 318 285 270 255
EcoRI G'AATT_C
Cuts at: 0 1417 2967
Size: 1417 1550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 105 735 2967
Size: 105 630 2232
Fragments arranged by size:
2232 630 105
HaeII r_GCGC'y
Cuts at: 0 51 380 492 1062 2967
Size: 51 329 112 570 1905
Fragments arranged by size:
1905 570 329 112 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 766 798 2164 2196 2967
Size: 766 32 1366 32 771
Fragments arranged by size:
1366 771 766 32 32
HincII GTy'rAC
Cuts at: 0 12 1002 1134 2673 2967
Size: 12 990 132 1539 294
Fragments arranged by size:
1539 990 294 132 12
HindIII A'AGCT_T
Cuts at: 0 860 2967
Size: 860 2107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 2837 2967
Size: 1022 1815 130
Fragments arranged by size:
1815 1022 130
MscI TGG'CCA
Cuts at: 0 2704 2905 2967
Size: 2704 201 62
Fragments arranged by size:
2704 201 62
MslI CAynn'nnrTG
Cuts at: 0 304 1657 2071 2851 2967
Size: 304 1353 414 780 116
Fragments arranged by size:
1353 780 414 304 116
MspA1I CmG'CkG
Cuts at: 0 457 798 1224 1935 2749 2967
Size: 457 341 426 711 814 218
Fragments arranged by size:
814 711 457 426 341 218
NaeI GCC'GGC
Cuts at: 0 444 2967
Size: 444 2523
NgoMIV G'CCGG_C
Cuts at: 0 442 2967
Size: 442 2525
NsiI A_TGCA'T
Cuts at: 0 352 2967
Size: 352 2615
NspI r_CATG'y
Cuts at: 0 309 697 819 1326 1599 2019 2328 2679
Size: 309 388 122 507 273 420 309 351
Cuts at: 2679 2967
Size: 288
Fragments arranged by size:
507 420 388 351 309 309 288 273
122
PasI CC'CwG_GG
Cuts at: 0 2928 2967
Size: 2928 39
PciI A'CATG_T
Cuts at: 0 305 2967
Size: 305 2662
PflMI CCAn_nnn'nTGG
Cuts at: 0 457 2967
Size: 457 2510
PfoI T'CCnGG_A
Cuts at: 0 845 1330 2006 2967
Size: 845 485 676 961
Fragments arranged by size:
961 845 676 485
PpuMI rG'GwC_Cy
Cuts at: 0 270 1785 2682 2967
Size: 270 1515 897 285
Fragments arranged by size:
1515 897 285 270
PshAI GACnn'nnGTC
Cuts at: 0 2341 2481 2967
Size: 2341 140 486
Fragments arranged by size:
2341 486 140
PspOMI G'GGCC_C
Cuts at: 0 524 1466 2967
Size: 524 942 1501
Fragments arranged by size:
1501 942 524
PspXI vC'TCGA_Gb
Cuts at: 0 748 2967
Size: 748 2219
PstI C_TGCA'G
Cuts at: 0 417 1922 1970 2036 2834 2967
Size: 417 1505 48 66 798 133
Fragments arranged by size:
1505 798 417 133 66 48
PvuII CAG'CTG
Cuts at: 0 798 1224 1935 2967
Size: 798 426 711 1032
Fragments arranged by size:
1032 798 711 426
RsrII CG'GwC_CG
Cuts at: 0 1161 2967
Size: 1161 1806
SacI G_AGCT'C
Cuts at: 0 908 2587 2967
Size: 908 1679 380
Fragments arranged by size:
1679 908 380
SanDI GG'GwC_CC
Cuts at: 0 2682 2967
Size: 2682 285
ScaI AGT'ACT
Cuts at: 0 967 2967
Size: 967 2000
SfcI C'TryA_G
Cuts at: 0 413 1918 1966 2032 2830 2967
Size: 413 1505 48 66 798 137
Fragments arranged by size:
1505 798 413 137 66 48
SmaI CCC'GGG
Cuts at: 0 2414 2967
Size: 2414 553
SmlI C'TyrA_G
Cuts at: 0 748 907 931 1261 2967
Size: 748 159 24 330 1706
Fragments arranged by size:
1706 748 330 159 24
SphI G_CATG'C
Cuts at: 0 697 819 1599 2967
Size: 697 122 780 1368
Fragments arranged by size:
1368 780 697 122
StuI AGG'CCT
Cuts at: 0 556 1729 2967
Size: 556 1173 1238
Fragments arranged by size:
1238 1173 556
StyI C'CwwG_G
Cuts at: 0 2147 2891 2967
Size: 2147 744 76
Fragments arranged by size:
2147 744 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1178 2642 2776 2967
Size: 1178 1464 134 191
Fragments arranged by size:
1464 1178 191 134
TatI w'GTAC_w
Cuts at: 0 965 1075 2967
Size: 965 110 1892
Fragments arranged by size:
1892 965 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 827 859 895 927 1312 1344 1547 1579
Size: 827 32 36 32 385 32 203 32
Cuts at: 1579 2002 2034 2967
Size: 423 32 933
Fragments arranged by size:
933 827 423 385 203 36 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 780 2967
Size: 780 2187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2316 2362 2892 2898 2967
Size: 2316 46 530 6 69
Fragments arranged by size:
2316 530 69 46 6
XhoI C'TCGA_G
Cuts at: 0 748 2967
Size: 748 2219
XmaI C'CCGG_G
Cuts at: 0 2412 2967
Size: 2412 555
XmnI GAAnn'nnTTC
Cuts at: 0 85 1387 1417 2967
Size: 85 1302 30 1550
Fragments arranged by size:
1550 1302 85 30
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PpuMI PshAI PspOMI PspXI PstI PvuII RsrII
SacI SanDI ScaI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BmtI BsaAI BsaBI BsiEI BsiWI BssHII BssSI
BstEII BstZ17I ClaI DraI EagI EcoRV FseI FspI
FspAI HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI PacI PmeI PmlI PpiI
PsiI PsrI PvuI SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI ZraI