Restriction map for
pF1KA0133
(Linear) (Six-base) MAPSORT of: KIAA0133.seq Check: 6955 from: 1 to: 4572
KIAA0133.orf
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 405 608 669 1025 4572
Size: 405 203 61 356 3547
Fragments arranged by size:
3547 405 356 203 61
AatII G_ACGT'C
Cuts at: 0 4457 4572
Size: 4457 115
AccI GT'mk_AC
Cuts at: 0 391 4572
Size: 391 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 268 1255 1280 2099 4023 4093 4572
Size: 205 63 987 25 819 1924 70 479
Fragments arranged by size:
1924 987 819 479 205 70 63 25
AflII C'TTAA_G
Cuts at: 0 28 4572
Size: 28 4544
AflIII A'CryG_T
Cuts at: 0 1535 3773 4358 4572
Size: 1535 2238 585 214
Fragments arranged by size:
2238 1535 585 214
AhdI GACnn_n'nnGTC
Cuts at: 0 1539 3763 4572
Size: 1539 2224 809
Fragments arranged by size:
2224 1539 809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 262 1610 1699 4572
Size: 262 1348 89 2873
Fragments arranged by size:
2873 1348 262 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1840 1872 2608 2640 4572
Size: 1840 32 736 32 1932
Fragments arranged by size:
1932 1840 736 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 432 684 1444 1530 1746 3225 3289 3483
Size: 432 252 760 86 216 1479 64 194
Cuts at: 3483 3637 3759 4464 4572
Size: 154 122 705 108
Fragments arranged by size:
1479 760 705 432 252 216 194 154
122 108 86 64
ApaI G_GGCC'C
Cuts at: 0 1480 1842 1898 3321 4572
Size: 1480 362 56 1423 1251
Fragments arranged by size:
1480 1423 1251 362 56
ApaLI G'TGCA_C
Cuts at: 0 4271 4572
Size: 4271 301
ApoI r'AATT_y
Cuts at: 0 1136 2986 3641 4572
Size: 1136 1850 655 931
Fragments arranged by size:
1850 1136 931 655
AseI AT'TA_AT
Cuts at: 0 278 4572
Size: 278 4294
AvaI C'yCGr_G
Cuts at: 0 1473 3320 4101 4572
Size: 1473 1847 781 471
Fragments arranged by size:
1847 1473 781 471
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2014 2047 4572
Size: 2014 33 2525
Fragments arranged by size:
2525 2014 33
BamHI G'GATC_C
Cuts at: 0 340 4572
Size: 340 4232
BanI G'GyrC_C
Cuts at: 0 2476 3329 4572
Size: 2476 853 1243
Fragments arranged by size:
2476 1243 853
BanII G_rGCy'C
Cuts at: 0 773 1480 1736 1842 1898 3321 4511 4572
Size: 773 707 256 106 56 1423 1190 61
Fragments arranged by size:
1423 1190 773 707 256 106 61 56
BbeI G_GCGC'C
Cuts at: 0 3333 4572
Size: 3333 1239
BbsI GAAGACnn'nnnn_
Cuts at: 0 805 1243 1359 4089 4572
Size: 805 438 116 2730 483
Fragments arranged by size:
2730 805 483 438 116
BbvCI CC'TCA_GC
Cuts at: 0 673 1445 3820 4316 4572
Size: 673 772 2375 496 256
Fragments arranged by size:
2375 772 673 496 256
BciVI GTATCCnnnnn_n'
Cuts at: 0 2272 4572
Size: 2272 2300
BclI T'GATC_A
Cuts at: 0 3513 4572
Size: 3513 1059
BglI GCCn_nnn'nGGC
Cuts at: 0 1348 1603 4572
Size: 1348 255 2969
Fragments arranged by size:
2969 1348 255
BglII A'GATC_T
Cuts at: 0 1919 4572
Size: 1919 2653
BlpI GC'TnA_GC
Cuts at: 0 448 685 1181 3049 4572
Size: 448 237 496 1868 1523
Fragments arranged by size:
1868 1523 496 448 237
Bme1580I G_kGCm'C
Cuts at: 0 646 1480 1842 1898 2481 3321 4275 4572
Size: 646 834 362 56 583 840 954 297
Fragments arranged by size:
954 840 834 646 583 362 297 56
BmgBI CAC'GTC
Cuts at: 0 574 4026 4572
Size: 574 3452 546
Fragments arranged by size:
3452 574 546
BmrI ACTGGGnnnn_n'
Cuts at: 0 495 4347 4572
Size: 495 3852 225
Fragments arranged by size:
3852 495 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1528 1573 1936 2442 2677 3736 4102
Size: 589 939 45 363 506 235 1059 366
Cuts at: 4102 4572
Size: 470
Fragments arranged by size:
1059 939 589 506 470 366 363 235
45
Bpu10I CC'TnA_GC
Cuts at: 0 673 743 1445 3820 4316 4572
Size: 673 70 702 2375 496 256
Fragments arranged by size:
2375 702 673 496 256 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 2800 3747 4510 4572
Size: 244 2556 947 763 62
Fragments arranged by size:
2556 947 763 244 62
BsaAI yAC'GTr
Cuts at: 0 1176 1538 4359 4572
Size: 1176 362 2821 213
Fragments arranged by size:
2821 1176 362 213
BsaBI GATnn'nnATC
Cuts at: 0 3034 4572
Size: 3034 1538
BsaHI Gr'CG_yC
Cuts at: 0 3330 4454 4572
Size: 3330 1124 118
Fragments arranged by size:
3330 1124 118
BsaWI w'CCGG_w
Cuts at: 0 2072 4572
Size: 2072 2500
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 479 509 4572
Size: 479 30 4063
Fragments arranged by size:
4063 479 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 740 1039 1674 2615 2790 2793 3031
Size: 247 493 299 635 941 175 3 238
Cuts at: 3031 4015 4333 4572
Size: 984 318 239
Fragments arranged by size:
984 941 635 493 318 299 247 239
238 175 3
BseYI C'CCAG_C
Cuts at: 0 2480 3225 3240 4572
Size: 2480 745 15 1332
Fragments arranged by size:
2480 1332 745 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1621 1759 3811 3965 4093 4572
Size: 893 728 138 2052 154 128 479
Fragments arranged by size:
2052 893 728 479 154 138 128
BsiHKAI G_wGCw'C
Cuts at: 0 1736 1820 2874 3062 4121 4275 4511 4572
Size: 1736 84 1054 188 1059 154 236 61
Fragments arranged by size:
1736 1059 1054 236 188 154 84 61
BsmI GAATG_Cn'
Cuts at: 0 2462 3082 4572
Size: 2462 620 1490
Fragments arranged by size:
2462 1490 620
Bsp1286I G_dGCh'C
Cuts at: 0 646 773 1480 1736 1820 1842 1898 2481
Size: 646 127 707 256 84 22 56 583
Cuts at: 2481 2874 3062 3321 4121 4275 4511 4572
Size: 393 188 259 800 154 236 61
Fragments arranged by size:
800 707 646 583 393 259 256 236
188 154 127 84 61 56 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 405 549 608 669 1025 1295 2042 3258
Size: 405 144 59 61 356 270 747 1216
Cuts at: 3258 4259 4572
Size: 1001 313
Fragments arranged by size:
1216 1001 747 405 356 313 270 144
61 59
BsrFI r'CCGG_y
Cuts at: 0 1215 4572
Size: 1215 3357
BsrGI T'GTAC_A
Cuts at: 0 4292 4572
Size: 4292 280
BssSI C'ACGA_G
Cuts at: 0 1735 4310 4572
Size: 1735 2575 262
Fragments arranged by size:
2575 1735 262
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1464 1746 3273 4116 4572
Size: 1464 282 1527 843 456
Fragments arranged by size:
1527 1464 843 456 282
BstBI TT'CG_AA
Cuts at: 0 1329 4572
Size: 1329 3243
BstEII G'GTnAC_C
Cuts at: 0 4377 4572
Size: 4377 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 909 1790 2845 4572
Size: 909 881 1055 1727
Fragments arranged by size:
1727 1055 909 881
BstYI r'GATC_y
Cuts at: 0 212 340 1526 1919 3633 4572
Size: 212 128 1186 393 1714 939
Fragments arranged by size:
1714 1186 939 393 212 128
Bsu36I CC'TnA_GG
Cuts at: 0 622 4145 4572
Size: 622 3523 427
Fragments arranged by size:
3523 622 427
BtgI C'CryG_G
Cuts at: 0 830 1399 1484 2312 2768 4572
Size: 830 569 85 828 456 1804
Fragments arranged by size:
1804 830 828 569 456 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1107 2120 4287 4572
Size: 1107 1013 2167 285
Fragments arranged by size:
2167 1107 1013 285
BtsI GCAGTG_nn'
Cuts at: 0 1638 3390 3748 3814 3982 4141 4572
Size: 1638 1752 358 66 168 159 431
Fragments arranged by size:
1752 1638 431 358 168 159 66
ClaI AT'CG_AT
Cuts at: 0 1307 4572
Size: 1307 3265
DraIII CAC_nnn'GTG
Cuts at: 0 3847 4116 4572
Size: 3847 269 456
Fragments arranged by size:
3847 456 269
EaeI y'GGCC_r
Cuts at: 0 419 531 660 899 1100 2172 2285 2771
Size: 419 112 129 239 201 1072 113 486
Cuts at: 2771 4572
Size: 1801
Fragments arranged by size:
1801 1072 486 419 239 201 129 113
112
EarI CTCTTCn'nnn_
Cuts at: 0 1142 1406 2339 3615 4126 4572
Size: 1142 264 933 1276 511 446
Fragments arranged by size:
1276 1142 933 511 446 264
EcoICRI GAG'CTC
Cuts at: 0 1734 4509 4572
Size: 1734 2775 63
Fragments arranged by size:
2775 1734 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 268 589 1255 1280 1528 1573 1936
Size: 205 63 321 666 25 248 45 363
Cuts at: 1936 2099 2442 2677 3736 4023 4093 4102 4572
Size: 163 343 235 1059 287 70 9 470
Fragments arranged by size:
1059 666 470 363 343 321 287 248
235 205 163 70 63 45 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1021 3460 4572
Size: 1021 2439 1112
Fragments arranged by size:
2439 1112 1021
EcoO109I rG'GnC_Cy
Cuts at: 0 1477 1895 3202 3237 3317 4572
Size: 1477 418 1307 35 80 1255
Fragments arranged by size:
1477 1307 1255 418 80 35
EcoRI G'AATT_C
Cuts at: 0 1136 2986 4572
Size: 1136 1850 1586
Fragments arranged by size:
1850 1586 1136
EcoRV GAT'ATC
Cuts at: 0 1247 4572
Size: 1247 3325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 148 2401 2658 3069 4398 4572
Size: 148 2253 257 411 1329 174
Fragments arranged by size:
2253 1329 411 257 174 148
HaeII r_GCGC'y
Cuts at: 0 1354 3333 4572
Size: 1354 1979 1239
Fragments arranged by size:
1979 1354 1239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 736 768 1419 1451 1525 1557
Size: 264 32 440 32 651 32 74 32
Cuts at: 1557 2398 2430 2955 2987 3194 3226 3749 3781
Size: 841 32 525 32 207 32 523 32
Cuts at: 3781 4371 4403 4572
Size: 590 32 169
Fragments arranged by size:
841 651 590 525 523 440 264 207
169 74 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 392 564 4572
Size: 392 172 4008
Fragments arranged by size:
4008 392 172
HindIII A'AGCT_T
Cuts at: 0 31 175 2139 3920 4572
Size: 31 144 1964 1781 652
Fragments arranged by size:
1964 1781 652 144 31
KasI G'GCGC_C
Cuts at: 0 3329 4572
Size: 3329 1243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1413 2651 3467 4343 4572
Size: 1413 1238 816 876 229
Fragments arranged by size:
1413 1238 876 816 229
MscI TGG'CCA
Cuts at: 0 533 662 901 1102 2174 2287 4572
Size: 533 129 239 201 1072 113 2285
Fragments arranged by size:
2285 1072 533 239 201 129 113
MslI CAynn'nnrTG
Cuts at: 0 2542 3040 3697 4572
Size: 2542 498 657 875
Fragments arranged by size:
2542 875 657 498
MspA1I CmG'CkG
Cuts at: 0 672 1229 1441 2028 2655 2715 3808 4080
Size: 672 557 212 587 627 60 1093 272
Cuts at: 4080 4393 4572
Size: 313 179
Fragments arranged by size:
1093 672 627 587 557 313 272 212
179 60
NarI GG'CG_CC
Cuts at: 0 3330 4572
Size: 3330 1242
NcoI C'CATG_G
Cuts at: 0 830 2312 2768 4572
Size: 830 1482 456 1804
Fragments arranged by size:
1804 1482 830 456
NspI r_CATG'y
Cuts at: 0 1502 3777 4572
Size: 1502 2275 795
Fragments arranged by size:
2275 1502 795
PasI CC'CwG_GG
Cuts at: 0 3150 4572
Size: 3150 1422
PciI A'CATG_T
Cuts at: 0 3773 4572
Size: 3773 799
PflMI CCAn_nnn'nTGG
Cuts at: 0 3198 3759 3847 4572
Size: 3198 561 88 725
Fragments arranged by size:
3198 725 561 88
PfoI T'CCnGG_A
Cuts at: 0 1505 1529 1760 4430 4572
Size: 1505 24 231 2670 142
Fragments arranged by size:
2670 1505 231 142 24
PmlI CAC'GTG
Cuts at: 0 1176 1538 4572
Size: 1176 362 3034
Fragments arranged by size:
3034 1176 362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1141 1173 4572
Size: 703 32 406 32 3399
Fragments arranged by size:
3399 703 406 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3202 4572
Size: 3202 1370
PshAI GACnn'nnGTC
Cuts at: 0 2492 3383 4572
Size: 2492 891 1189
Fragments arranged by size:
2492 1189 891
PsiI TTA'TAA
Cuts at: 0 167 4572
Size: 167 4405
PspOMI G'GGCC_C
Cuts at: 0 1476 1838 1894 3317 4572
Size: 1476 362 56 1423 1255
Fragments arranged by size:
1476 1423 1255 362 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 113 145 4572
Size: 113 32 4427
Fragments arranged by size:
4427 113 32
PstI C_TGCA'G
Cuts at: 0 475 554 1022 1799 2039 3266 3281 3299
Size: 475 79 468 777 240 1227 15 18
Cuts at: 3299 3397 4136 4412 4481 4572
Size: 98 739 276 69 91
Fragments arranged by size:
1227 777 739 475 468 276 240 98
91 79 69 18 15
PvuII CAG'CTG
Cuts at: 0 672 1441 2028 2655 2715 3808 4393 4572
Size: 672 769 587 627 60 1093 585 179
Fragments arranged by size:
1093 769 672 627 587 585 179 60
SacI G_AGCT'C
Cuts at: 0 1736 4511 4572
Size: 1736 2775 61
Fragments arranged by size:
2775 1736 61
SalI G'TCGA_C
Cuts at: 0 390 4572
Size: 390 4182
SapI GCTCTTCn'nnn_
Cuts at: 0 4126 4572
Size: 4126 446
SbfI CC_TGCA'GG
Cuts at: 0 475 1022 4572
Size: 475 547 3550
Fragments arranged by size:
3550 547 475
SexAI A'CCwGG_T
Cuts at: 0 3181 4572
Size: 3181 1391
SfcI C'TryA_G
Cuts at: 0 471 550 1018 1795 1869 2035 2207 3262
Size: 471 79 468 777 74 166 172 1055
Cuts at: 3262 3277 3295 3393 4132 4408 4477 4572
Size: 15 18 98 739 276 69 95
Fragments arranged by size:
1055 777 739 471 468 276 172 166
98 95 79 74 69 18 15
SfoI GGC'GCC
Cuts at: 0 3331 4572
Size: 3331 1241
SmaI CCC'GGG
Cuts at: 0 3322 4572
Size: 3322 1250
SmlI C'TyrA_G
Cuts at: 0 28 259 2815 3726 4525 4572
Size: 28 231 2556 911 799 47
Fragments arranged by size:
2556 911 799 231 47 28
SpeI A'CTAG_T
Cuts at: 0 2065 4572
Size: 2065 2507
SphI G_CATG'C
Cuts at: 0 1502 4572
Size: 1502 3070
StyI C'CwwG_G
Cuts at: 0 528 830 2312 2768 3420 4572
Size: 528 302 1482 456 652 1152
Fragments arranged by size:
1482 1152 652 528 456 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1224 3330 3709 4572
Size: 1224 2106 379 863
Fragments arranged by size:
2106 1224 863 379
TatI w'GTAC_w
Cuts at: 0 2919 4292 4572
Size: 2919 1373 280
Fragments arranged by size:
2919 1373 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2175 2207 4572
Size: 2175 32 2365
Fragments arranged by size:
2365 2175 32
XbaI T'CTAG_A
Cuts at: 0 933 4572
Size: 933 3639
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1068 4572
Size: 1068 3504
XmaI C'CCGG_G
Cuts at: 0 3320 4572
Size: 3320 1252
XmnI GAAnn'nnTTC
Cuts at: 0 4183 4572
Size: 4183 389
ZraI GAC'GTC
Cuts at: 0 4455 4572
Size: 4455 117
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SacI SalI SapI
SbfI SexAI SfcI SfoI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI AscI AsiSI AvrII
BcgI BfrBI BmtI BplI BsaI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BssHII BstZ17I CspCI DraI
DrdI EagI EciI FseI FspI FspAI HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PspXI PvuI RsrII SacII SanDI
ScaI SfiI SgfI SgrAI SnaBI SrfI SspI StuI
SwaI Tth111I XhoI