Restriction map for
pF1KA0138
(Linear) (Six-base) MAPSORT of: KIAA0138.seq Check: 5934 from: 1 to: 2859
KIAA0138.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 1366 1523 2859
Size: 1366 157 1336
Fragments arranged by size:
1366 1336 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 298 431 1631 2859
Size: 145 153 133 1200 1228
Fragments arranged by size:
1228 1200 153 145 133
AhdI GACnn_n'nnGTC
Cuts at: 0 1833 2859
Size: 1833 1026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 734 977 2859
Size: 734 243 1882
Fragments arranged by size:
1882 734 243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1880 1912 2859
Size: 1880 32 947
Fragments arranged by size:
1880 947 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2028 2859
Size: 2028 831
ApaI G_GGCC'C
Cuts at: 0 37 2663 2859
Size: 37 2626 196
Fragments arranged by size:
2626 196 37
AvaI C'yCGr_G
Cuts at: 0 16 30 57 1332 1951 2008 2179 2664
Size: 16 14 27 1275 619 57 171 485
Cuts at: 2664 2859
Size: 195
Fragments arranged by size:
1275 619 485 195 171 57 27 16
14
BanI G'GyrC_C
Cuts at: 0 2138 2590 2748 2859
Size: 2138 452 158 111
Fragments arranged by size:
2138 452 158 111
BanII G_rGCy'C
Cuts at: 0 37 887 977 1001 1147 1341 1742 2663
Size: 37 850 90 24 146 194 401 921
Cuts at: 2663 2859
Size: 196
Fragments arranged by size:
921 850 401 196 194 146 90 37
24
BbeI G_GCGC'C
Cuts at: 0 2142 2859
Size: 2142 717
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 1058 1619 2859
Size: 323 735 561 1240
Fragments arranged by size:
1240 735 561 323
BclI T'GATC_A
Cuts at: 0 1131 2859
Size: 1131 1728
BglI GCCn_nnn'nGGC
Cuts at: 0 933 2667 2859
Size: 933 1734 192
Fragments arranged by size:
1734 933 192
BlpI GC'TnA_GC
Cuts at: 0 88 524 2859
Size: 88 436 2335
Fragments arranged by size:
2335 436 88
Bme1580I G_kGCm'C
Cuts at: 0 37 2663 2678 2859
Size: 37 2626 15 181
Fragments arranged by size:
2626 181 37 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 83 2547 2859
Size: 83 2464 312
Fragments arranged by size:
2464 312 83
BmtI G_CTAG'C
Cuts at: 0 2615 2859
Size: 2615 244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1800 2859
Size: 1800 1059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 416 727 2050 2080 2859
Size: 379 37 311 1323 30 779
Fragments arranged by size:
1323 779 379 311 37 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1252 2859
Size: 163 1089 1607
Fragments arranged by size:
1607 1089 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 2306 2369 2859
Size: 2306 63 490
Fragments arranged by size:
2306 490 63
BsaAI yAC'GTr
Cuts at: 0 495 2745 2859
Size: 495 2250 114
Fragments arranged by size:
2250 495 114
BsaHI Gr'CG_yC
Cuts at: 0 2139 2859
Size: 2139 720
BsaWI w'CCGG_w
Cuts at: 0 603 792 2859
Size: 603 189 2067
Fragments arranged by size:
2067 603 189
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1727 1757 2859
Size: 1727 30 1102
Fragments arranged by size:
1727 1102 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 1009 2143 2578 2859
Size: 301 708 1134 435 281
Fragments arranged by size:
1134 708 435 301 281
BseYI C'CCAG_C
Cuts at: 0 1462 2462 2859
Size: 1462 1000 397
Fragments arranged by size:
1462 1000 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 2285 2859
Size: 485 1800 574
Fragments arranged by size:
1800 574 485
BsiEI CG_ry'CG
Cuts at: 0 1715 2445 2859
Size: 1715 730 414
Fragments arranged by size:
1715 730 414
BsiHKAI G_wGCw'C
Cuts at: 0 432 977 1001 1147 2549 2570 2624 2859
Size: 432 545 24 146 1402 21 54 235
Fragments arranged by size:
1402 545 432 235 146 54 24 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 323 2859
Size: 323 2536
Bsp1286I G_dGCh'C
Cuts at: 0 37 432 887 977 1001 1147 1341 1742
Size: 37 395 455 90 24 146 194 401
Cuts at: 1742 2549 2570 2624 2663 2678 2859
Size: 807 21 54 39 15 181
Fragments arranged by size:
807 455 401 395 194 181 146 90
54 39 37 24 21 15
BspEI T'CCGG_A
Cuts at: 0 603 792 2859
Size: 603 189 2067
Fragments arranged by size:
2067 603 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 1757 2859
Size: 374 1383 1102
Fragments arranged by size:
1383 1102 374
BsrBI CCG'CTC
Cuts at: 0 174 1707 1938 1968 2034 2049 2055 2172
Size: 174 1533 231 30 66 15 6 117
Cuts at: 2172 2859
Size: 687
Fragments arranged by size:
1533 687 231 174 117 66 30 15
6
BsrDI GCAATG_nn'
Cuts at: 0 2252 2859
Size: 2252 607
BsrFI r'CCGG_y
Cuts at: 0 942 2859
Size: 942 1917
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2386 2859
Size: 2386 473
BstYI r'GATC_y
Cuts at: 0 207 1263 1895 2859
Size: 207 1056 632 964
Fragments arranged by size:
1056 964 632 207
BtgI C'CryG_G
Cuts at: 0 887 1013 2412 2418 2439 2594 2688 2859
Size: 887 126 1399 6 21 155 94 171
Fragments arranged by size:
1399 887 171 155 126 94 21 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 917 2859
Size: 917 1942
BtsI GCAGTG_nn'
Cuts at: 0 432 2859
Size: 432 2427
ClaI AT'CG_AT
Cuts at: 0 107 2859
Size: 107 2752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 719 754 2859
Size: 719 35 2105
Fragments arranged by size:
2105 719 35
DraIII CAC_nnn'GTG
Cuts at: 0 2725 2748 2859
Size: 2725 23 111
Fragments arranged by size:
2725 111 23
EaeI y'GGCC_r
Cuts at: 0 815 2224 2278 2415 2442 2529 2780 2859
Size: 815 1409 54 137 27 87 251 79
Fragments arranged by size:
1409 815 251 137 87 79 54 27
EagI C'GGCC_G
Cuts at: 0 2442 2859
Size: 2442 417
EarI CTCTTCn'nnn_
Cuts at: 0 191 761 791 1571 2859
Size: 191 570 30 780 1288
Fragments arranged by size:
1288 780 570 191 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 133 2199 2774 2789 2859
Size: 19 114 2066 575 15 70
Fragments arranged by size:
2066 575 114 70 19 15
EcoICRI GAG'CTC
Cuts at: 0 975 999 1145 2859
Size: 975 24 146 1714
Fragments arranged by size:
1714 975 146 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 298 379 416 431 727 1631 2050
Size: 145 153 81 37 15 296 904 419
Cuts at: 2050 2080 2859
Size: 30 779
Fragments arranged by size:
904 779 419 296 153 145 81 37
30 15
EcoO109I rG'GnC_Cy
Cuts at: 0 34 771 2189 2425 2649 2660 2859
Size: 34 737 1418 236 224 11 199
Fragments arranged by size:
1418 737 236 224 199 34 11
EcoRV GAT'ATC
Cuts at: 0 2260 2859
Size: 2260 599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 637 2859
Size: 637 2222
HaeII r_GCGC'y
Cuts at: 0 788 1130 1875 2022 2142 2439 2859
Size: 788 342 745 147 120 297 420
Fragments arranged by size:
788 745 420 342 297 147 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1415 1447 1727 1759 1782 1789 1814 1819
Size: 1415 32 280 32 23 7 25 5
Cuts at: 1819 1821 1851 2859
Size: 2 30 1008
Fragments arranged by size:
1415 1008 280 32 32 30 25 23
7 5 2
HincII GTy'rAC
Cuts at: 0 827 1367 1524 2859
Size: 827 540 157 1335
Fragments arranged by size:
1335 827 540 157
HindIII A'AGCT_T
Cuts at: 0 754 1471 2859
Size: 754 717 1388
Fragments arranged by size:
1388 754 717
KasI G'GCGC_C
Cuts at: 0 2138 2859
Size: 2138 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1198 1502 2504 2859
Size: 1198 304 1002 355
Fragments arranged by size:
1198 1002 355 304
MscI TGG'CCA
Cuts at: 0 817 2226 2417 2782 2859
Size: 817 1409 191 365 77
Fragments arranged by size:
1409 817 365 191 77
MslI CAynn'nnrTG
Cuts at: 0 394 1750 2077 2384 2859
Size: 394 1356 327 307 475
Fragments arranged by size:
1356 475 394 327 307
MspA1I CmG'CkG
Cuts at: 0 507 889 1208 1235 1884 1932 1992 2025
Size: 507 382 319 27 649 48 60 33
Cuts at: 2025 2154 2556 2690 2859
Size: 129 402 134 169
Fragments arranged by size:
649 507 402 382 319 169 134 129
60 48 33 27
NaeI GCC'GGC
Cuts at: 0 944 2859
Size: 944 1915
NarI GG'CG_CC
Cuts at: 0 2139 2859
Size: 2139 720
NcoI C'CATG_G
Cuts at: 0 2412 2594 2859
Size: 2412 182 265
Fragments arranged by size:
2412 265 182
NgoMIV G'CCGG_C
Cuts at: 0 942 2859
Size: 942 1917
NheI G'CTAG_C
Cuts at: 0 2611 2859
Size: 2611 248
NspI r_CATG'y
Cuts at: 0 541 2076 2859
Size: 541 1535 783
Fragments arranged by size:
1535 783 541
PasI CC'CwG_GG
Cuts at: 0 2522 2736 2859
Size: 2522 214 123
Fragments arranged by size:
2522 214 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 439 904 2028 2859
Size: 439 465 1124 831
Fragments arranged by size:
1124 831 465 439
PmlI CAC'GTG
Cuts at: 0 2745 2859
Size: 2745 114
PpuMI rG'GwC_Cy
Cuts at: 0 771 2425 2859
Size: 771 1654 434
Fragments arranged by size:
1654 771 434
PspOMI G'GGCC_C
Cuts at: 0 33 2659 2859
Size: 33 2626 200
Fragments arranged by size:
2626 200 33
PspXI vC'TCGA_Gb
Cuts at: 0 1951 2008 2859
Size: 1951 57 851
Fragments arranged by size:
1951 851 57
PstI C_TGCA'G
Cuts at: 0 371 2006 2859
Size: 371 1635 853
Fragments arranged by size:
1635 853 371
PvuII CAG'CTG
Cuts at: 0 507 2154 2859
Size: 507 1647 705
Fragments arranged by size:
1647 705 507
SacI G_AGCT'C
Cuts at: 0 977 1001 1147 2859
Size: 977 24 146 1712
Fragments arranged by size:
1712 977 146 24
SacII CC_GC'GG
Cuts at: 0 890 2691 2859
Size: 890 1801 168
Fragments arranged by size:
1801 890 168
SalI G'TCGA_C
Cuts at: 0 1365 1522 2859
Size: 1365 157 1337
Fragments arranged by size:
1365 1337 157
SfcI C'TryA_G
Cuts at: 0 367 929 1987 2002 2859
Size: 367 562 1058 15 857
Fragments arranged by size:
1058 857 562 367 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2667 2859
Size: 2667 192
SfoI GGC'GCC
Cuts at: 0 2140 2859
Size: 2140 719
SmaI CCC'GGG
Cuts at: 0 18 59 1334 2181 2859
Size: 18 41 1275 847 678
Fragments arranged by size:
1275 847 678 41 18
SmlI C'TyrA_G
Cuts at: 0 178 1267 1951 2008 2859
Size: 178 1089 684 57 851
Fragments arranged by size:
1089 851 684 178 57
SphI G_CATG'C
Cuts at: 0 541 2076 2859
Size: 541 1535 783
Fragments arranged by size:
1535 783 541
SrfI GCCC'GGGC
Cuts at: 0 59 2181 2859
Size: 59 2122 678
Fragments arranged by size:
2122 678 59
StuI AGG'CCT
Cuts at: 0 985 1957 2859
Size: 985 972 902
Fragments arranged by size:
985 972 902
StyI C'CwwG_G
Cuts at: 0 2412 2594 2859
Size: 2412 182 265
Fragments arranged by size:
2412 265 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1026 1658 2219 2270 2828 2859
Size: 1026 632 561 51 558 31
Fragments arranged by size:
1026 632 561 558 51 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 906 938 2225 2257 2859
Size: 906 32 1287 32 602
Fragments arranged by size:
1287 906 602 32 32
XbaI T'CTAG_A
Cuts at: 0 1672 2859
Size: 1672 1187
XhoI C'TCGA_G
Cuts at: 0 1951 2008 2859
Size: 1951 57 851
Fragments arranged by size:
1951 851 57
XmaI C'CCGG_G
Cuts at: 0 16 57 1332 2179 2859
Size: 16 41 1275 847 680
Fragments arranged by size:
1275 847 680 41 16
XmnI GAAnn'nnTTC
Cuts at: 0 1276 2859
Size: 1276 1583
Enzymes that do cut:
AccI AcuI AhdI AlfI AloI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI
PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SalI SfcI SfiI SfoI SmaI SmlI SphI SrfI
StuI StyI TaqII TstI XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglII BmgBI Bpu10I
BsaBI BsiWI BsmI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I DraI DrdI EcoNI EcoRI
FseI FspI FspAI HpaI KpnI MfeI MluI NdeI
NotI NruI NsiI PacI PciI PfoI PmeI PpiI
PshAI PsiI PsrI PvuI RsrII SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SspI SwaI
TatI Tth111I XcmI ZraI