Restriction map for
pF1KSDA0141
(Linear) (Six-base) MAPSORT of: KIAA0141.seq Check: 863 from: 1 to: 1545
KIAA0141.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
Acc65I G'GTAC_C
Cuts at: 0 1163 1545
Size: 1163 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1363 1545
Size: 1045 318 182
Fragments arranged by size:
1045 318 182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 860 1545
Size: 860 685
ApaI G_GGCC'C
Cuts at: 0 1319 1545
Size: 1319 226
ApoI r'AATT_y
Cuts at: 0 1175 1545
Size: 1175 370
AvaI C'yCGr_G
Cuts at: 0 12 1069 1311 1416 1545
Size: 12 1057 242 105 129
Fragments arranged by size:
1057 242 129 105 12
BanI G'GyrC_C
Cuts at: 0 5 398 874 962 1163 1545
Size: 5 393 476 88 201 382
Fragments arranged by size:
476 393 382 201 88 5
BanII G_rGCy'C
Cuts at: 0 33 546 1094 1287 1319 1545
Size: 33 513 548 193 32 226
Fragments arranged by size:
548 513 226 193 33 32
BbeI G_GCGC'C
Cuts at: 0 9 1545
Size: 9 1536
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 1545
Size: 212 1333
BbvCI CC'TCA_GC
Cuts at: 0 335 501 638 1545
Size: 335 166 137 907
Fragments arranged by size:
907 335 166 137
BglI GCCn_nnn'nGGC
Cuts at: 0 280 443 1545
Size: 280 163 1102
Fragments arranged by size:
1102 280 163
BlpI GC'TnA_GC
Cuts at: 0 506 1545
Size: 506 1039
Bme1580I G_kGCm'C
Cuts at: 0 268 1319 1545
Size: 268 1051 226
Fragments arranged by size:
1051 268 226
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 1545
Size: 55 1490
BmtI G_CTAG'C
Cuts at: 0 1390 1545
Size: 1390 155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 560 1545
Size: 86 474 985
Fragments arranged by size:
985 474 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1338 1464 1545
Size: 81 1257 126 81
Fragments arranged by size:
1257 126 81 81
Bpu10I CC'TnA_GC
Cuts at: 0 335 501 638 998 1545
Size: 335 166 137 360 547
Fragments arranged by size:
547 360 335 166 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1068 1245 1545
Size: 706 362 177 300
Fragments arranged by size:
706 362 300 177
BsaI GGTCTCn'nnnn_
Cuts at: 0 968 1545
Size: 968 577
BsaHI Gr'CG_yC
Cuts at: 0 6 1545
Size: 6 1539
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 413 421 443 1545
Size: 391 22 8 22 1102
Fragments arranged by size:
1102 391 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 399 554 703 1429 1545
Size: 99 300 155 149 726 116
Fragments arranged by size:
726 300 155 149 116 99
BseYI C'CCAG_C
Cuts at: 0 433 447 632 978 1318 1545
Size: 433 14 185 346 340 227
Fragments arranged by size:
433 346 340 227 185 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1545
Size: 1222 323
BsiHKAI G_wGCw'C
Cuts at: 0 33 546 1423 1545
Size: 33 513 877 122
Fragments arranged by size:
877 513 122 33
Bsp1286I G_dGCh'C
Cuts at: 0 33 268 546 1094 1287 1319 1423 1545
Size: 33 235 278 548 193 32 104 122
Fragments arranged by size:
548 278 235 193 122 104 33 32
BspHI T'CATG_A
Cuts at: 0 858 1545
Size: 858 687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1151 1545
Size: 1151 394
BsrBI CCG'CTC
Cuts at: 0 173 1002 1249 1545
Size: 173 829 247 296
Fragments arranged by size:
829 296 247 173
BssSI C'ACGA_G
Cuts at: 0 168 1545
Size: 168 1377
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 1545
Size: 120 1425
BstYI r'GATC_y
Cuts at: 0 302 1545
Size: 302 1243
Bsu36I CC'TnA_GG
Cuts at: 0 469 551 1545
Size: 469 82 994
Fragments arranged by size:
994 469 82
BtgI C'CryG_G
Cuts at: 0 156 189 819 1295 1545
Size: 156 33 630 476 250
Fragments arranged by size:
630 476 250 156 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 1545
Size: 720 825
BtsI GCAGTG_nn'
Cuts at: 0 348 412 1019 1545
Size: 348 64 607 526
Fragments arranged by size:
607 526 348 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 75 1545
Size: 75 1470
EaeI y'GGCC_r
Cuts at: 0 272 486 1545
Size: 272 214 1059
Fragments arranged by size:
1059 272 214
EarI CTCTTCn'nnn_
Cuts at: 0 38 721 763 990 1545
Size: 38 683 42 227 555
Fragments arranged by size:
683 555 227 42 38
EcoICRI GAG'CTC
Cuts at: 0 31 544 1545
Size: 31 513 1001
Fragments arranged by size:
1001 513 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1045 1338 1363 1464 1545
Size: 81 964 293 25 101 81
Fragments arranged by size:
964 293 101 81 81 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 1482 1545
Size: 688 794 63
Fragments arranged by size:
794 688 63
EcoO109I rG'GnC_Cy
Cuts at: 0 49 151 185 331 555 571 1315 1545
Size: 49 102 34 146 224 16 744 230
Fragments arranged by size:
744 230 224 146 102 49 34 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 222 1545
Size: 222 1323
HaeII r_GCGC'y
Cuts at: 0 9 1545
Size: 9 1536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 41 73 162 194 241 273 410 442
Size: 41 32 89 32 47 32 137 32
Cuts at: 442 967 999 1545
Size: 525 32 546
Fragments arranged by size:
546 525 137 89 47 41 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1061 1545
Size: 1061 484
KasI G'GCGC_C
Cuts at: 0 5 1545
Size: 5 1540
KpnI G_GTAC'C
Cuts at: 0 1167 1545
Size: 1167 378
MscI TGG'CCA
Cuts at: 0 488 1545
Size: 488 1057
MslI CAynn'nnrTG
Cuts at: 0 863 1545
Size: 863 682
MspA1I CmG'CkG
Cuts at: 0 291 339 821 1545
Size: 291 48 482 724
Fragments arranged by size:
724 482 291 48
NarI GG'CG_CC
Cuts at: 0 6 1545
Size: 6 1539
NcoI C'CATG_G
Cuts at: 0 156 189 1295 1545
Size: 156 33 1106 250
Fragments arranged by size:
1106 250 156 33
NheI G'CTAG_C
Cuts at: 0 1386 1545
Size: 1386 159
NspI r_CATG'y
Cuts at: 0 598 1414 1545
Size: 598 816 131
Fragments arranged by size:
816 598 131
PasI CC'CwG_GG
Cuts at: 0 881 1545
Size: 881 664
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 939 1545
Size: 260 679 606
Fragments arranged by size:
679 606 260
PpuMI rG'GwC_Cy
Cuts at: 0 49 151 185 331 555 571 1545
Size: 49 102 34 146 224 16 974
Fragments arranged by size:
974 224 146 102 49 34 16
PshAI GACnn'nnGTC
Cuts at: 0 1344 1545
Size: 1344 201
PspOMI G'GGCC_C
Cuts at: 0 1315 1545
Size: 1315 230
PspXI vC'TCGA_Gb
Cuts at: 0 1416 1545
Size: 1416 129
PstI C_TGCA'G
Cuts at: 0 290 816 1038 1308 1372 1447 1545
Size: 290 526 222 270 64 75 98
Fragments arranged by size:
526 290 270 222 98 75 64
PvuII CAG'CTG
Cuts at: 0 291 1545
Size: 291 1254
SacI G_AGCT'C
Cuts at: 0 33 546 1545
Size: 33 513 999
Fragments arranged by size:
999 513 33
SacII CC_GC'GG
Cuts at: 0 822 1545
Size: 822 723
SapI GCTCTTCn'nnn_
Cuts at: 0 38 721 1545
Size: 38 683 824
Fragments arranged by size:
824 683 38
SfcI C'TryA_G
Cuts at: 0 286 812 825 1034 1304 1368 1443 1545
Size: 286 526 13 209 270 64 75 102
Fragments arranged by size:
526 286 270 209 102 75 64 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 280 1545
Size: 280 1265
SfoI GGC'GCC
Cuts at: 0 7 1545
Size: 7 1538
SmaI CCC'GGG
Cuts at: 0 14 1313 1545
Size: 14 1299 232
Fragments arranged by size:
1299 232 14
SmlI C'TyrA_G
Cuts at: 0 685 1047 1224 1416 1545
Size: 685 362 177 192 129
Fragments arranged by size:
685 362 192 177 129
SphI G_CATG'C
Cuts at: 0 598 1545
Size: 598 947
StuI AGG'CCT
Cuts at: 0 1193 1222 1545
Size: 1193 29 323
Fragments arranged by size:
1193 323 29
StyI C'CwwG_G
Cuts at: 0 156 181 189 1085 1170 1194 1295 1431
Size: 156 25 8 896 85 24 101 136
Cuts at: 1431 1545
Size: 114
Fragments arranged by size:
896 156 136 114 101 85 25 24
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 416 1545
Size: 416 1129
TatI w'GTAC_w
Cuts at: 0 839 1545
Size: 839 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 1545
Size: 391 32 1122
Fragments arranged by size:
1122 391 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 986 1545
Size: 986 559
XhoI C'TCGA_G
Cuts at: 0 1416 1545
Size: 1416 129
XmaI C'CCGG_G
Cuts at: 0 12 1311 1545
Size: 12 1299 234
Fragments arranged by size:
1299 234 12
XmnI GAAnn'nnTTC
Cuts at: 0 1348 1545
Size: 1348 197
Enzymes that do cut:
Acc65I AcuI AlfI ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BssSI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HindIII KasI KpnI MscI MslI MspA1I NarI NcoI
NheI NspI PasI PflMI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SapI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmgBI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HpaI MfeI MluI
MmeI NaeI NdeI NgoMIV NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PsiI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI