Restriction map for
pF1KSDA0142
(Linear) (Six-base) MAPSORT of: KIAA0142.seq Check: 2167 from: 1 to: 1938
KIAA0142.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 95 1938
Size: 95 1843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 319 1529 1918 1938
Size: 250 69 1210 389 20
Fragments arranged by size:
1210 389 250 69 20
AflIII A'CryG_T
Cuts at: 0 911 1938
Size: 911 1027
AleI CACnn'nnGTG
Cuts at: 0 109 1207 1938
Size: 109 1098 731
Fragments arranged by size:
1098 731 109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 496 1938
Size: 193 303 1442
Fragments arranged by size:
1442 303 193
AlwNI CAG_nnn'CTG
Cuts at: 0 849 924 1161 1605 1938
Size: 849 75 237 444 333
Fragments arranged by size:
849 444 333 237 75
AvaI C'yCGr_G
Cuts at: 0 724 1938
Size: 724 1214
AvrII C'CTAG_G
Cuts at: 0 1009 1938
Size: 1009 929
BanI G'GyrC_C
Cuts at: 0 634 1354 1938
Size: 634 720 584
Fragments arranged by size:
720 634 584
BanII G_rGCy'C
Cuts at: 0 238 725 1495 1863 1938
Size: 238 487 770 368 75
Fragments arranged by size:
770 487 368 238 75
BbvCI CC'TCA_GC
Cuts at: 0 1455 1938
Size: 1455 483
BciVI GTATCCnnnnn_n'
Cuts at: 0 1767 1938
Size: 1767 171
BfrBI ATG'CAT
Cuts at: 0 1099 1786 1938
Size: 1099 687 152
Fragments arranged by size:
1099 687 152
BglI GCCn_nnn'nGGC
Cuts at: 0 155 1938
Size: 155 1783
BlpI GC'TnA_GC
Cuts at: 0 491 1938
Size: 491 1447
Bme1580I G_kGCm'C
Cuts at: 0 139 1938
Size: 139 1799
BmgBI CAC'GTC
Cuts at: 0 1205 1938
Size: 1205 733
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 1386 1938
Size: 879 507 552
Fragments arranged by size:
879 552 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1408 1434 1894 1938
Size: 1408 26 460 44
Fragments arranged by size:
1408 460 44 26
Bpu10I CC'TnA_GC
Cuts at: 0 673 1420 1455 1938
Size: 673 747 35 483
Fragments arranged by size:
747 673 483 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1093 1938
Size: 856 237 845
Fragments arranged by size:
856 845 237
BsaI GGTCTCn'nnnn_
Cuts at: 0 619 1921 1938
Size: 619 1302 17
Fragments arranged by size:
1302 619 17
BsaAI yAC'GTr
Cuts at: 0 177 554 1938
Size: 177 377 1384
Fragments arranged by size:
1384 377 177
BsaHI Gr'CG_yC
Cuts at: 0 92 1315 1938
Size: 92 1223 623
Fragments arranged by size:
1223 623 92
BsaWI w'CCGG_w
Cuts at: 0 818 863 1938
Size: 818 45 1075
Fragments arranged by size:
1075 818 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1546 1576 1938
Size: 1546 30 362
Fragments arranged by size:
1546 362 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 1567 1938
Size: 619 948 371
Fragments arranged by size:
948 619 371
BseYI C'CCAG_C
Cuts at: 0 166 1938
Size: 166 1772
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1611 1938
Size: 1611 327
BsiEI CG_ry'CG
Cuts at: 0 150 1938
Size: 150 1788
BsiHKAI G_wGCw'C
Cuts at: 0 725 1181 1938
Size: 725 456 757
Fragments arranged by size:
757 725 456
BsmI GAATG_Cn'
Cuts at: 0 475 1938
Size: 475 1463
BsmBI CGTCTCn'nnnn_
Cuts at: 0 83 1938
Size: 83 1855
Bsp1286I G_dGCh'C
Cuts at: 0 139 238 725 1181 1495 1863 1938
Size: 139 99 487 456 314 368 75
Fragments arranged by size:
487 456 368 314 139 99 75
BspEI T'CCGG_A
Cuts at: 0 818 863 1938
Size: 818 45 1075
Fragments arranged by size:
1075 818 45
BsrBI CCG'CTC
Cuts at: 0 1128 1938
Size: 1128 810
BsrDI GCAATG_nn'
Cuts at: 0 364 1938
Size: 364 1574
BsrFI r'CCGG_y
Cuts at: 0 154 1938
Size: 154 1784
BssSI C'ACGA_G
Cuts at: 0 655 1938
Size: 655 1283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1161 1938
Size: 1161 777
BstYI r'GATC_y
Cuts at: 0 845 1158 1479 1938
Size: 845 313 321 459
Fragments arranged by size:
845 459 321 313
BtgI C'CryG_G
Cuts at: 0 665 776 1350 1938
Size: 665 111 574 588
Fragments arranged by size:
665 588 574 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 893 1938
Size: 893 1045
BtsI GCAGTG_nn'
Cuts at: 0 584 1938
Size: 584 1354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1673 1708 1938
Size: 1673 35 230
Fragments arranged by size:
1673 230 35
DraI TTT'AAA
Cuts at: 0 518 1938
Size: 518 1420
EaeI y'GGCC_r
Cuts at: 0 147 361 1938
Size: 147 214 1577
Fragments arranged by size:
1577 214 147
EagI C'GGCC_G
Cuts at: 0 147 1938
Size: 147 1791
EarI CTCTTCn'nnn_
Cuts at: 0 110 226 817 979 1700 1938
Size: 110 116 591 162 721 238
Fragments arranged by size:
721 591 238 162 116 110
EcoICRI GAG'CTC
Cuts at: 0 723 1938
Size: 723 1215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 319 1408 1434 1529 1894 1918 1938
Size: 250 69 1089 26 95 365 24 20
Fragments arranged by size:
1089 365 250 95 69 26 24 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 242 1938
Size: 242 1696
EcoO109I rG'GnC_Cy
Cuts at: 0 921 1381 1938
Size: 921 460 557
Fragments arranged by size:
921 557 460
EcoRV GAT'ATC
Cuts at: 0 964 1938
Size: 964 974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 1711 1938
Size: 63 1648 227
Fragments arranged by size:
1648 227 63
HaeII r_GCGC'y
Cuts at: 0 1637 1938
Size: 1637 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 1546 1578 1938
Size: 80 32 1434 32 360
Fragments arranged by size:
1434 360 80 32 32
HindIII A'AGCT_T
Cuts at: 0 1039 1069 1619 1938
Size: 1039 30 550 319
Fragments arranged by size:
1039 550 319 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 1069 1938
Size: 483 586 869
Fragments arranged by size:
869 586 483
MslI CAynn'nnrTG
Cuts at: 0 109 910 1094 1207 1938
Size: 109 801 184 113 731
Fragments arranged by size:
801 731 184 113 109
MspA1I CmG'CkG
Cuts at: 0 1311 1459 1579 1938
Size: 1311 148 120 359
Fragments arranged by size:
1311 359 148 120
NcoI C'CATG_G
Cuts at: 0 776 1938
Size: 776 1162
NsiI A_TGCA'T
Cuts at: 0 1101 1788 1938
Size: 1101 687 150
Fragments arranged by size:
1101 687 150
NspI r_CATG'y
Cuts at: 0 915 1938
Size: 915 1023
PasI CC'CwG_GG
Cuts at: 0 646 1938
Size: 646 1292
PciI A'CATG_T
Cuts at: 0 911 1938
Size: 911 1027
PflMI CCAn_nnn'nTGG
Cuts at: 0 1376 1412 1938
Size: 1376 36 526
Fragments arranged by size:
1376 526 36
PfoI T'CCnGG_A
Cuts at: 0 1564 1938
Size: 1564 374
PpuMI rG'GwC_Cy
Cuts at: 0 921 1381 1938
Size: 921 460 557
Fragments arranged by size:
921 557 460
PstI C_TGCA'G
Cuts at: 0 579 845 1166 1938
Size: 579 266 321 772
Fragments arranged by size:
772 579 321 266
PvuII CAG'CTG
Cuts at: 0 1459 1579 1938
Size: 1459 120 359
Fragments arranged by size:
1459 359 120
RsrII CG'GwC_CG
Cuts at: 0 152 1938
Size: 152 1786
SacI G_AGCT'C
Cuts at: 0 725 1938
Size: 725 1213
SanDI GG'GwC_CC
Cuts at: 0 1381 1938
Size: 1381 557
SapI GCTCTTCn'nnn_
Cuts at: 0 226 1938
Size: 226 1712
SfcI C'TryA_G
Cuts at: 0 292 575 841 1162 1183 1938
Size: 292 283 266 321 21 755
Fragments arranged by size:
755 321 292 283 266 21
SmlI C'TyrA_G
Cuts at: 0 724 835 1072 1938
Size: 724 111 237 866
Fragments arranged by size:
866 724 237 111
SspI AAT'ATT
Cuts at: 0 300 322 1938
Size: 300 22 1616
Fragments arranged by size:
1616 300 22
StyI C'CwwG_G
Cuts at: 0 776 1009 1938
Size: 776 233 929
Fragments arranged by size:
929 776 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 1161 1419 1938
Size: 19 1142 258 519
Fragments arranged by size:
1142 519 258 19
XbaI T'CTAG_A
Cuts at: 0 420 1842 1938
Size: 420 1422 96
Fragments arranged by size:
1422 420 96
XhoI C'TCGA_G
Cuts at: 0 724 1938
Size: 724 1214
ZraI GAC'GTC
Cuts at: 0 93 1938
Size: 93 1845
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI AvaI AvrII
BanI BanII BbvCI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssSI BstAPI
BstYI BtgI BtgZI BtsI CspCI DraI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HindIII MmeI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PfoI PpuMI PstI PvuII RsrII
SacI SanDI SapI SfcI SmlI SspI StyI TaqII
XbaI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BcgI BclI BglII BmtI BplI
BsaBI BsiWI BspHI BspMI BsrGI BssHII BstBI BstEII
BstXI BstZ17I Bsu36I ClaI DraIII DrdI EciI EcoRI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI Tth111I XcmI XmaI
XmnI