Restriction map for
pF1KA0143
(Linear) (Six-base) MAPSORT of: KIAA0143.seq Check: 2011 from: 1 to: 2463
KIAA0143.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AclI AA'CG_TT
Cuts at: 0 1628 2463
Size: 1628 835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 1905 2189 2463
Size: 95 1810 284 274
Fragments arranged by size:
1810 284 274 95
AhdI GACnn_n'nnGTC
Cuts at: 0 1432 2463
Size: 1432 1031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 840 2463
Size: 840 1623
AlwNI CAG_nnn'CTG
Cuts at: 0 136 951 2463
Size: 136 815 1512
Fragments arranged by size:
1512 815 136
ApoI r'AATT_y
Cuts at: 0 360 373 811 1048 1292 1541 1877 2260
Size: 360 13 438 237 244 249 336 383
Cuts at: 2260 2313 2463
Size: 53 150
Fragments arranged by size:
438 383 360 336 249 244 237 150
53 13
AvaI C'yCGr_G
Cuts at: 0 8 923 2463
Size: 8 915 1540
Fragments arranged by size:
1540 915 8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1573 1606 2463
Size: 1573 33 857
Fragments arranged by size:
1573 857 33
BamHI G'GATC_C
Cuts at: 0 801 1383 2463
Size: 801 582 1080
Fragments arranged by size:
1080 801 582
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 1002 1354 1628 2463
Size: 398 604 352 274 835
Fragments arranged by size:
835 604 398 352 274
BbvCI CC'TCA_GC
Cuts at: 0 572 2463
Size: 572 1891
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 2463
Size: 33 2430
BciVI GTATCCnnnnn_n'
Cuts at: 0 2118 2463
Size: 2118 345
BfrBI ATG'CAT
Cuts at: 0 1449 2463
Size: 1449 1014
BglII A'GATC_T
Cuts at: 0 1312 2448 2463
Size: 1312 1136 15
Fragments arranged by size:
1312 1136 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 2363 2419 2463
Size: 2363 56 44
Fragments arranged by size:
2363 56 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 2463
Size: 125 2338
Bpu10I CC'TnA_GC
Cuts at: 0 572 2463
Size: 572 1891
BsaBI GATnn'nnATC
Cuts at: 0 2069 2463
Size: 2069 394
BsaHI Gr'CG_yC
Cuts at: 0 2244 2463
Size: 2244 219
BsaWI w'CCGG_w
Cuts at: 0 1506 2463
Size: 1506 957
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2354 2463
Size: 2354 109
BseYI C'CCAG_C
Cuts at: 0 723 2463
Size: 723 1740
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1346 2463
Size: 1153 193 1117
Fragments arranged by size:
1153 1117 193
BsmI GAATG_Cn'
Cuts at: 0 1143 2463
Size: 1143 1320
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1042 2463
Size: 1042 1421
BspHI T'CATG_A
Cuts at: 0 1202 1467 1565 2322 2463
Size: 1202 265 98 757 141
Fragments arranged by size:
1202 757 265 141 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 925 2463
Size: 925 1538
BsrDI GCAATG_nn'
Cuts at: 0 968 1724 2463
Size: 968 756 739
Fragments arranged by size:
968 756 739
BstAPI GCAn_nnn'nTGC
Cuts at: 0 136 1801 2463
Size: 136 1665 662
Fragments arranged by size:
1665 662 136
BstBI TT'CG_AA
Cuts at: 0 1052 1881 2463
Size: 1052 829 582
Fragments arranged by size:
1052 829 582
BstEII G'GTnAC_C
Cuts at: 0 1326 2463
Size: 1326 1137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1783 2118 2217 2463
Size: 1783 335 99 246
Fragments arranged by size:
1783 335 246 99
BstYI r'GATC_y
Cuts at: 0 78 801 1075 1312 1383 2448 2463
Size: 78 723 274 237 71 1065 15
Fragments arranged by size:
1065 723 274 237 78 71 15
BtsI GCAGTG_nn'
Cuts at: 0 822 1092 1363 2463
Size: 822 270 271 1100
Fragments arranged by size:
1100 822 271 270
DraI TTT'AAA
Cuts at: 0 831 2463
Size: 831 1632
DraIII CAC_nnn'GTG
Cuts at: 0 873 2299 2463
Size: 873 1426 164
Fragments arranged by size:
1426 873 164
EaeI y'GGCC_r
Cuts at: 0 1721 2463
Size: 1721 742
EarI CTCTTCn'nnn_
Cuts at: 0 674 2463
Size: 674 1789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 125 1905 2189 2463
Size: 95 30 1780 284 274
Fragments arranged by size:
1780 284 274 95 30
EcoO109I rG'GnC_Cy
Cuts at: 0 646 2463
Size: 646 1817
EcoRI G'AATT_C
Cuts at: 0 1048 1292 2463
Size: 1048 244 1171
Fragments arranged by size:
1171 1048 244
EcoRV GAT'ATC
Cuts at: 0 1260 2463
Size: 1260 1203
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 2092 2463
Size: 109 1983 371
Fragments arranged by size:
1983 371 109
FspI TGC'GCA
Cuts at: 0 776 2463
Size: 776 1687
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 1418 1450 2020 2052 2092 2113
Size: 385 32 1001 32 570 32 40 21
Cuts at: 2113 2124 2145 2463
Size: 11 21 318
Fragments arranged by size:
1001 570 385 318 40 32 32 32
21 21 11
HincII GTy'rAC
Cuts at: 0 540 633 1092 1267 2463
Size: 540 93 459 175 1196
Fragments arranged by size:
1196 540 459 175 93
HindIII A'AGCT_T
Cuts at: 0 294 340 1483 2463
Size: 294 46 1143 980
Fragments arranged by size:
1143 980 294 46
MfeI C'AATT_G
Cuts at: 0 2201 2463
Size: 2201 262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 2156 2463
Size: 1018 1138 307
Fragments arranged by size:
1138 1018 307
MscI TGG'CCA
Cuts at: 0 1723 2463
Size: 1723 740
MslI CAynn'nnrTG
Cuts at: 0 899 1781 2116 2463
Size: 899 882 335 347
Fragments arranged by size:
899 882 347 335
MspA1I CmG'CkG
Cuts at: 0 1587 2463
Size: 1587 876
NdeI CA'TA_TG
Cuts at: 0 305 578 2463
Size: 305 273 1885
Fragments arranged by size:
1885 305 273
NsiI A_TGCA'T
Cuts at: 0 1451 2463
Size: 1451 1012
NspI r_CATG'y
Cuts at: 0 615 1861 1938 2407 2463
Size: 615 1246 77 469 56
Fragments arranged by size:
1246 615 469 77 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 88 563 1254 2343 2463
Size: 88 475 691 1089 120
Fragments arranged by size:
1089 691 475 120 88
PfoI T'CCnGG_A
Cuts at: 0 878 1379 2463
Size: 878 501 1084
Fragments arranged by size:
1084 878 501
PsiI TTA'TAA
Cuts at: 0 838 1672 2463
Size: 838 834 791
Fragments arranged by size:
838 834 791
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2339 2371 2463
Size: 2339 32 92
Fragments arranged by size:
2339 92 32
PvuII CAG'CTG
Cuts at: 0 1587 2463
Size: 1587 876
SfcI C'TryA_G
Cuts at: 0 1079 2463
Size: 1079 1384
SmaI CCC'GGG
Cuts at: 0 925 2463
Size: 925 1538
SphI G_CATG'C
Cuts at: 0 1938 2407 2463
Size: 1938 469 56
Fragments arranged by size:
1938 469 56
SspI AAT'ATT
Cuts at: 0 384 2342 2463
Size: 384 1958 121
Fragments arranged by size:
1958 384 121
TatI w'GTAC_w
Cuts at: 0 1973 2463
Size: 1973 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2343 2375 2463
Size: 2343 32 88
Fragments arranged by size:
2343 88 32
XbaI T'CTAG_A
Cuts at: 0 2080 2463
Size: 2080 383
XmaI C'CCGG_G
Cuts at: 0 923 2463
Size: 923 1540
XmnI GAAnn'nnTTC
Cuts at: 0 162 296 360 2463
Size: 162 134 64 2103
Fragments arranged by size:
2103 162 134 64
Enzymes that do cut:
AclI AcuI AhdI AlfI AlwNI ApoI AvaI BaeI
BamHI BbsI BbvCI BcgI BciVI BfrBI BglII BmrI
BpmI Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsmI BsmBI BspHI BspMI BsrDI BstAPI BstBI BstEII
BstXI BstYI BtsI DraI DraIII EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI
PflMI PfoI PsiI PsrI PvuII SfcI SmaI SphI
SspI TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BanI BanII BbeI BclI BglI BlpI Bme1580I BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaXI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI
EciI EcoICRI EcoNI FseI FspAI HaeII HpaI KasI
KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI StuI StyI
SwaI TaqII Tth111I XcmI XhoI ZraI