Restriction map for
pF1KA0144
(Linear) (Six-base) MAPSORT of: KIAA0144.seq Check: 4555 from: 1 to: 2949
KIAA0144.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1326 2949
Size: 1326 1623
AccI GT'mk_AC
Cuts at: 0 1648 1732 2949
Size: 1648 84 1217
Fragments arranged by size:
1648 1217 84
Acc65I G'GTAC_C
Cuts at: 0 1802 2949
Size: 1802 1147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 779 968 1288 1394 1845 2333 2461
Size: 758 21 189 320 106 451 488 128
Cuts at: 2461 2949
Size: 488
Fragments arranged by size:
758 488 488 451 320 189 128 106
21
AhdI GACnn_n'nnGTC
Cuts at: 0 1419 2949
Size: 1419 1530
AleI CACnn'nnGTG
Cuts at: 0 2162 2164 2949
Size: 2162 2 785
Fragments arranged by size:
2162 785 2
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1452 1579 1921 2230 2949
Size: 939 513 127 342 309 719
Fragments arranged by size:
939 719 513 342 309 127
ApaI G_GGCC'C
Cuts at: 0 1768 2949
Size: 1768 1181
ApaLI G'TGCA_C
Cuts at: 0 1240 2949
Size: 1240 1709
AvaI C'yCGr_G
Cuts at: 0 29 2751 2949
Size: 29 2722 198
Fragments arranged by size:
2722 198 29
AvrII C'CTAG_G
Cuts at: 0 2295 2949
Size: 2295 654
BamHI G'GATC_C
Cuts at: 0 906 2949
Size: 906 2043
BanI G'GyrC_C
Cuts at: 0 427 475 1802 2949
Size: 427 48 1327 1147
Fragments arranged by size:
1327 1147 427 48
BanII G_rGCy'C
Cuts at: 0 30 1180 1768 2949
Size: 30 1150 588 1181
Fragments arranged by size:
1181 1150 588 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 785 874 1349 2949
Size: 785 89 475 1600
Fragments arranged by size:
1600 785 475 89
BbvCI CC'TCA_GC
Cuts at: 0 1433 2290 2949
Size: 1433 857 659
Fragments arranged by size:
1433 857 659
BciVI GTATCCnnnnn_n'
Cuts at: 0 2884 2949
Size: 2884 65
BfrBI ATG'CAT
Cuts at: 0 2845 2949
Size: 2845 104
BglII A'GATC_T
Cuts at: 0 753 770 2949
Size: 753 17 2179
Fragments arranged by size:
2179 753 17
BlpI GC'TnA_GC
Cuts at: 0 1130 1442 1521 2949
Size: 1130 312 79 1428
Fragments arranged by size:
1428 1130 312 79
Bme1580I G_kGCm'C
Cuts at: 0 19 1244 1768 2949
Size: 19 1225 524 1181
Fragments arranged by size:
1225 1181 524 19
BmgBI CAC'GTC
Cuts at: 0 400 2949
Size: 400 2549
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 1074 2236 2549 2949
Size: 48 1026 1162 313 400
Fragments arranged by size:
1162 1026 400 313 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2181 2244 2402 2949
Size: 2181 63 158 547
Fragments arranged by size:
2181 547 158 63
Bpu10I CC'TnA_GC
Cuts at: 0 1433 1760 2290 2949
Size: 1433 327 530 659
Fragments arranged by size:
1433 659 530 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 2055 2949
Size: 563 1492 894
Fragments arranged by size:
1492 894 563
BsaI GGTCTCn'nnnn_
Cuts at: 0 365 757 2888 2949
Size: 365 392 2131 61
Fragments arranged by size:
2131 392 365 61
BsaAI yAC'GTr
Cuts at: 0 662 2949
Size: 662 2287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1371 1401 1795 1825 2535 2565 2949
Size: 1371 30 394 30 710 30 384
Fragments arranged by size:
1371 710 394 384 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 575 739 1152 1352 2062 2247 2351
Size: 166 409 164 413 200 710 185 104
Cuts at: 2351 2601 2949
Size: 250 348
Fragments arranged by size:
710 413 409 348 250 200 185 166
164 104
BseYI C'CCAG_C
Cuts at: 0 600 861 932 1807 2616 2761 2949
Size: 600 261 71 875 809 145 188
Fragments arranged by size:
875 809 600 261 188 145 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 1325 1891 2216 2949
Size: 1225 100 566 325 733
Fragments arranged by size:
1225 733 566 325 100
BsiEI CG_ry'CG
Cuts at: 0 2598 2949
Size: 2598 351
BsiHKAI G_wGCw'C
Cuts at: 0 1244 2949
Size: 1244 1705
BsmI GAATG_Cn'
Cuts at: 0 279 1134 2013 2847 2949
Size: 279 855 879 834 102
Fragments arranged by size:
879 855 834 279 102
BsmBI CGTCTCn'nnnn_
Cuts at: 0 917 2949
Size: 917 2032
Bsp1286I G_dGCh'C
Cuts at: 0 19 30 1180 1244 1768 2949
Size: 19 11 1150 64 524 1181
Fragments arranged by size:
1181 1150 524 64 19 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1326 1553 2949
Size: 1326 227 1396
Fragments arranged by size:
1396 1326 227
BsrBI CCG'CTC
Cuts at: 0 1718 2949
Size: 1718 1231
BsrDI GCAATG_nn'
Cuts at: 0 231 1558 2949
Size: 231 1327 1391
Fragments arranged by size:
1391 1327 231
BsrFI r'CCGG_y
Cuts at: 0 1437 2949
Size: 1437 1512
BssSI C'ACGA_G
Cuts at: 0 1611 2949
Size: 1611 1338
BstBI TT'CG_AA
Cuts at: 0 955 2949
Size: 955 1994
BstEII G'GTnAC_C
Cuts at: 0 2578 2949
Size: 2578 371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 2634 2949
Size: 328 2306 315
Fragments arranged by size:
2306 328 315
BstYI r'GATC_y
Cuts at: 0 753 770 906 1405 1783 2949
Size: 753 17 136 499 378 1166
Fragments arranged by size:
1166 753 499 378 136 17
BstZ17I GTA'TAC
Cuts at: 0 1649 2949
Size: 1649 1300
BtgI C'CryG_G
Cuts at: 0 435 528 2598 2949
Size: 435 93 2070 351
Fragments arranged by size:
2070 435 351 93
BtsI GCAGTG_nn'
Cuts at: 0 92 776 827 1244 1328 1931 2949
Size: 92 684 51 417 84 603 1018
Fragments arranged by size:
1018 684 603 417 92 84 51
EaeI y'GGCC_r
Cuts at: 0 82 333 531 675 1658 2558 2595 2624
Size: 82 251 198 144 983 900 37 29
Cuts at: 2624 2849 2949
Size: 225 100
Fragments arranged by size:
983 900 251 225 198 144 100 82
37 29
EagI C'GGCC_G
Cuts at: 0 2595 2949
Size: 2595 354
EarI CTCTTCn'nnn_
Cuts at: 0 495 1626 1824 2814 2949
Size: 495 1131 198 990 135
Fragments arranged by size:
1131 990 495 198 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 779 968 1288 1394 1845 2181 2244
Size: 758 21 189 320 106 451 336 63
Cuts at: 2244 2333 2402 2461 2949
Size: 89 69 59 488
Fragments arranged by size:
758 488 451 336 320 189 106 89
69 63 59 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 983 1843 2949
Size: 983 860 1106
Fragments arranged by size:
1106 983 860
EcoO109I rG'GnC_Cy
Cuts at: 0 490 1764 1765 1836 2382 2748 2949
Size: 490 1274 1 71 546 366 201
Fragments arranged by size:
1274 546 490 366 201 71 1
EcoRV GAT'ATC
Cuts at: 0 1530 2949
Size: 1530 1419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1875 2949
Size: 1875 1074
HaeII r_GCGC'y
Cuts at: 0 1263 1619 2704 2949
Size: 1263 356 1085 245
Fragments arranged by size:
1263 1085 356 245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1162 1194 1405 1437 1482 1514 1582 1614
Size: 1162 32 211 32 45 32 68 32
Cuts at: 1614 1992 2024 2133 2165 2596 2628 2649 2681
Size: 378 32 109 32 431 32 21 32
Cuts at: 2681 2949
Size: 268
Fragments arranged by size:
1162 431 378 268 211 109 68 45
32 32 32 32 32 32 32 32
21
HincII GTy'rAC
Cuts at: 0 237 1733 2949
Size: 237 1496 1216
Fragments arranged by size:
1496 1216 237
KpnI G_GTAC'C
Cuts at: 0 1806 2949
Size: 1806 1143
MfeI C'AATT_G
Cuts at: 0 166 2949
Size: 166 2783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2886 2949
Size: 2886 63
MscI TGG'CCA
Cuts at: 0 335 677 2560 2949
Size: 335 342 1883 389
Fragments arranged by size:
1883 389 342 335
MslI CAynn'nnrTG
Cuts at: 0 137 2162 2164 2949
Size: 137 2025 2 785
Fragments arranged by size:
2025 785 137 2
MspA1I CmG'CkG
Cuts at: 0 81 604 653 1339 2535 2949
Size: 81 523 49 686 1196 414
Fragments arranged by size:
1196 686 523 414 81 49
NaeI GCC'GGC
Cuts at: 0 1439 2949
Size: 1439 1510
NgoMIV G'CCGG_C
Cuts at: 0 1437 2949
Size: 1437 1512
NsiI A_TGCA'T
Cuts at: 0 2847 2949
Size: 2847 102
NspI r_CATG'y
Cuts at: 0 1023 2434 2949
Size: 1023 1411 515
Fragments arranged by size:
1411 1023 515
PflMI CCAn_nnn'nTGG
Cuts at: 0 694 939 1086 1113 2949
Size: 694 245 147 27 1836
Fragments arranged by size:
1836 694 245 147 27
PpuMI rG'GwC_Cy
Cuts at: 0 1836 2382 2748 2949
Size: 1836 546 366 201
Fragments arranged by size:
1836 546 366 201
PshAI GACnn'nnGTC
Cuts at: 0 389 2949
Size: 389 2560
PspOMI G'GGCC_C
Cuts at: 0 1764 2949
Size: 1764 1185
PstI C_TGCA'G
Cuts at: 0 99 1323 1550 1926 1986 2949
Size: 99 1224 227 376 60 963
Fragments arranged by size:
1224 963 376 227 99 60
PvuII CAG'CTG
Cuts at: 0 604 1339 2949
Size: 604 735 1610
Fragments arranged by size:
1610 735 604
SalI G'TCGA_C
Cuts at: 0 1731 2949
Size: 1731 1218
SanDI GG'GwC_CC
Cuts at: 0 2382 2748 2949
Size: 2382 366 201
Fragments arranged by size:
2382 366 201
ScaI AGT'ACT
Cuts at: 0 1068 2949
Size: 1068 1881
SfcI C'TryA_G
Cuts at: 0 95 387 1070 1319 1546 1913 1922 1982
Size: 95 292 683 249 227 367 9 60
Cuts at: 1982 2502 2715 2949
Size: 520 213 234
Fragments arranged by size:
683 520 367 292 249 234 227 213
95 60 9
SmaI CCC'GGG
Cuts at: 0 31 2753 2949
Size: 31 2722 196
Fragments arranged by size:
2722 196 31
SmlI C'TyrA_G
Cuts at: 0 578 2034 2949
Size: 578 1456 915
Fragments arranged by size:
1456 915 578
StyI C'CwwG_G
Cuts at: 0 2024 2295 2633 2949
Size: 2024 271 338 316
Fragments arranged by size:
2024 338 316 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2017 2538 2949
Size: 2017 521 411
Fragments arranged by size:
2017 521 411
TatI w'GTAC_w
Cuts at: 0 1066 2949
Size: 1066 1883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1371 1403 2949
Size: 1371 32 1546
Fragments arranged by size:
1546 1371 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 328 2869 2949
Size: 328 2541 80
Fragments arranged by size:
2541 328 80
XmaI C'CCGG_G
Cuts at: 0 29 2751 2949
Size: 29 2722 198
Fragments arranged by size:
2722 198 29
XmnI GAAnn'nnTTC
Cuts at: 0 570 1858 2949
Size: 570 1288 1091
Fragments arranged by size:
1288 1091 570
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlwNI ApaI
ApaLI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII KpnI MfeI MmeI MscI
MslI MspA1I NaeI NgoMIV NsiI NspI PflMI PpuMI
PshAI PspOMI PstI PvuII SalI SanDI ScaI SfcI
SmaI SmlI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AlfI AloI
ApoI AscI AseI AsiSI BaeI BbeI BcgI BclI
BglI BmtI BplI BsaBI BsaHI BsaWI BsiWI BspEI
BspHI BsrGI BssHII BstAPI Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoRI FseI FspI
FspAI HindIII HpaI KasI MluI NarI NcoI NdeI
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI ZraI