Restriction map for
pF1KSDA0147
(Linear) (Six-base) MAPSORT of: KIAA0147.seq Check: 7140 from: 1 to: 4890
KIAA0147.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1243 4890
Size: 1243 3647
AccI GT'mk_AC
Cuts at: 0 818 4890
Size: 818 4072
Acc65I G'GTAC_C
Cuts at: 0 3846 4890
Size: 3846 1044
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 814 2066 2604 3811 4838 4890
Size: 318 496 1252 538 1207 1027 52
Fragments arranged by size:
1252 1207 1027 538 496 318 52
AfeI AGC'GCT
Cuts at: 0 3828 4890
Size: 3828 1062
AflIII A'CryG_T
Cuts at: 0 263 677 4890
Size: 263 414 4213
Fragments arranged by size:
4213 414 263
AhdI GACnn_n'nnGTC
Cuts at: 0 1506 4890
Size: 1506 3384
AleI CACnn'nnGTG
Cuts at: 0 2416 4648 4890
Size: 2416 2232 242
Fragments arranged by size:
2416 2232 242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2434 3169 4890
Size: 2434 735 1721
Fragments arranged by size:
2434 1721 735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 3290 3322 4890
Size: 334 32 2924 32 1568
Fragments arranged by size:
2924 1568 334 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 426 759 1116 1350 1504 1594 3492 3738
Size: 426 333 357 234 154 90 1898 246
Cuts at: 3738 3789 4209 4228 4890
Size: 51 420 19 662
Fragments arranged by size:
1898 662 426 420 357 333 246 234
154 90 51 19
ApaI G_GGCC'C
Cuts at: 0 1615 1915 1971 2567 2857 3037 3706 3752
Size: 1615 300 56 596 290 180 669 46
Cuts at: 3752 4117 4476 4595 4816 4879 4890
Size: 365 359 119 221 63 11
Fragments arranged by size:
1615 669 596 365 359 300 290 221
180 119 63 56 46 11
AseI AT'TA_AT
Cuts at: 0 2753 4890
Size: 2753 2137
AvaI C'yCGr_G
Cuts at: 0 226 430 646 970 985 1270 1606 1732
Size: 226 204 216 324 15 285 336 126
Cuts at: 1732 1864 1924 1964 2282 2309 2488 2507 2560
Size: 132 60 40 318 27 179 19 53
Cuts at: 2560 2935 3285 3751 4311 4441 4683 4890
Size: 375 350 466 560 130 242 207
Fragments arranged by size:
560 466 375 350 336 324 318 285
242 226 216 207 204 179 132 130
126 60 53 40 27 19 15
BanI G'GyrC_C
Cuts at: 0 659 2368 2940 3279 3315 3352 3846 4363
Size: 659 1709 572 339 36 37 494 517
Cuts at: 4363 4394 4437 4862 4890
Size: 31 43 425 28
Fragments arranged by size:
1709 659 572 517 494 425 339 43
37 36 31 28
BanII G_rGCy'C
Cuts at: 0 473 585 647 716 1442 1607 1615 1700
Size: 473 112 62 69 726 165 8 85
Cuts at: 1700 1915 1962 1971 2242 2567 2857 3037 3421
Size: 215 47 9 271 325 290 180 384
Cuts at: 3421 3697 3706 3752 4117 4318 4336 4469 4476
Size: 276 9 46 365 201 18 133 7
Cuts at: 4476 4595 4816 4879 4890
Size: 119 221 63 11
Fragments arranged by size:
726 473 384 365 325 290 276 271
221 215 201 180 165 133 119 112
85 69 63 62 47 46 18 11
9 9 8 7
BbeI G_GCGC'C
Cuts at: 0 663 2372 4367 4398 4866 4890
Size: 663 1709 1995 31 468 24
Fragments arranged by size:
1995 1709 663 468 31 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 1571 1679 4661 4890
Size: 1571 108 2982 229
Fragments arranged by size:
2982 1571 229 108
BbvCI CC'TCA_GC
Cuts at: 0 1379 1622 4558 4709 4890
Size: 1379 243 2936 151 181
Fragments arranged by size:
2936 1379 243 181 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1059 1093 2683 2717 4474 4508 4890
Size: 1059 34 1590 34 1757 34 382
Fragments arranged by size:
1757 1590 1059 382 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1876 2392 2484 2593 3368 3497 3701 3964
Size: 1876 516 92 109 775 129 204 263
Cuts at: 3964 3973 4480 4890
Size: 9 507 410
Fragments arranged by size:
1876 775 516 507 410 263 204 129
109 92 9
BglII A'GATC_T
Cuts at: 0 89 1844 4890
Size: 89 1755 3046
Fragments arranged by size:
3046 1755 89
BlpI GC'TnA_GC
Cuts at: 0 710 1601 3691 4890
Size: 710 891 2090 1199
Fragments arranged by size:
2090 1199 891 710
Bme1580I G_kGCm'C
Cuts at: 0 1615 1915 1971 2567 2722 2857 3037 3231
Size: 1615 300 56 596 155 135 180 194
Cuts at: 3231 3706 3752 3824 3983 4103 4117 4452 4476
Size: 475 46 72 159 120 14 335 24
Cuts at: 4476 4585 4595 4816 4879 4886 4890
Size: 109 10 221 63 7 4
Fragments arranged by size:
1615 596 475 335 300 221 194 180
159 155 135 120 109 72 63 56
46 24 14 10 7 4
BmgBI CAC'GTC
Cuts at: 0 264 438 678 1092 2766 3195 4890
Size: 264 174 240 414 1674 429 1695
Fragments arranged by size:
1695 1674 429 414 264 240 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 2213 2995 3753 4890
Size: 2213 782 758 1137
Fragments arranged by size:
2213 1137 782 758
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 888 3687 4890
Size: 888 2799 1203
Fragments arranged by size:
2799 1203 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 336 340 487 715 1329 1372 2336
Size: 133 203 4 147 228 614 43 964
Cuts at: 2336 2362 3268 3619 3682 3875 3884 4108 4498
Size: 26 906 351 63 193 9 224 390
Cuts at: 4498 4618 4890
Size: 120 272
Fragments arranged by size:
964 906 614 390 351 272 228 224
203 193 147 133 120 63 43 26
9 4
Bpu10I CC'TnA_GC
Cuts at: 0 196 1379 1622 4121 4558 4709 4890
Size: 196 1183 243 2499 437 151 181
Fragments arranged by size:
2499 1183 437 243 196 181 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1044 1129 1426 4308 4890
Size: 472 572 85 297 2882 582
Fragments arranged by size:
2882 582 572 472 297 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 1297 4890
Size: 1297 3593
BsaAI yAC'GTr
Cuts at: 0 39 1083 2583 4890
Size: 39 1044 1500 2307
Fragments arranged by size:
2307 1500 1044 39
BsaHI Gr'CG_yC
Cuts at: 0 131 660 1676 2369 4339 4364 4395 4689
Size: 131 529 1016 693 1970 25 31 294
Cuts at: 4689 4863 4890
Size: 174 27
Fragments arranged by size:
1970 1016 693 529 294 174 131 31
27 25
BsaWI w'CCGG_w
Cuts at: 0 614 959 1187 3650 3927 4425 4698 4890
Size: 614 345 228 2463 277 498 273 192
Fragments arranged by size:
2463 614 498 345 277 273 228 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 2309 2339 2750 2779 2780 2809
Size: 334 30 1945 30 411 29 1 29
Cuts at: 2809 4039 4069 4705 4735 4890
Size: 1230 30 636 30 155
Fragments arranged by size:
1945 1230 636 411 334 155 30 30
30 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 130 712 1470 1540 1543 1768 1952
Size: 100 30 582 758 70 3 225 184
Cuts at: 1952 1993 1996 1999 2002 2026 2029 2038 2041
Size: 41 3 3 3 24 3 9 3
Cuts at: 2041 2044 2083 2086 2182 3022 4183 4288 4726
Size: 3 39 3 96 840 1161 105 438
Cuts at: 4726 4771 4890
Size: 45 119
Fragments arranged by size:
1161 840 758 582 438 225 184 119
105 100 96 70 45 41 39 30
24 9 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 189 1250 1279 1432 1859 2613 2848 2866
Size: 189 1061 29 153 427 754 235 18
Cuts at: 2866 2965 3028 3330 3495 3823 4013 4228 4266
Size: 99 63 302 165 328 190 215 38
Cuts at: 4266 4738 4890
Size: 472 152
Fragments arranged by size:
1061 754 472 427 328 302 235 215
190 189 165 153 152 99 63 38
29 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 2440 3541 3724 3859 4438 4854 4890
Size: 1063 1377 1101 183 135 579 416 36
Fragments arranged by size:
1377 1101 1063 579 416 183 135 36
BsiEI CG_ry'CG
Cuts at: 0 2739 3447 3684 3977 4890
Size: 2739 708 237 293 913
Fragments arranged by size:
2739 913 708 293 237
BsiHKAI G_wGCw'C
Cuts at: 0 473 647 716 1232 1442 2378 3125 4469
Size: 473 174 69 516 210 936 747 1344
Cuts at: 4469 4548 4890
Size: 79 342
Fragments arranged by size:
1344 936 747 516 473 342 210 174
79 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2685 2799 4255 4890
Size: 2685 114 1456 635
Fragments arranged by size:
2685 1456 635 114
Bsp1286I G_dGCh'C
Cuts at: 0 473 585 647 716 1232 1442 1607 1615
Size: 473 112 62 69 516 210 165 8
Cuts at: 1615 1700 1915 1962 1971 2242 2378 2567 2722
Size: 85 215 47 9 271 136 189 155
Cuts at: 2722 2857 3037 3125 3231 3421 3697 3706 3752
Size: 135 180 88 106 190 276 9 46
Cuts at: 3752 3824 3983 4103 4117 4318 4336 4452 4469
Size: 72 159 120 14 201 18 116 17
Cuts at: 4469 4476 4548 4585 4595 4816 4879 4886 4890
Size: 7 72 37 10 221 63 7 4
Fragments arranged by size:
516 473 276 271 221 215 210 201
190 189 180 165 159 155 136 135
120 116 112 106 88 85 72 72
69 63 62 47 46 37 18 17
14 10 9 9 8 7 7 4
BspEI T'CCGG_A
Cuts at: 0 3927 4425 4890
Size: 3927 498 465
Fragments arranged by size:
3927 498 465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 491 662 749 767 905 1243 2150 2718
Size: 491 171 87 18 138 338 907 568
Cuts at: 2718 3760 4160 4553 4890
Size: 1042 400 393 337
Fragments arranged by size:
1042 907 568 491 400 393 338 337
171 138 87 18
BsrBI CCG'CTC
Cuts at: 0 917 2514 2841 3142 4068 4407 4890
Size: 917 1597 327 301 926 339 483
Fragments arranged by size:
1597 926 917 483 339 327 301
BsrDI GCAATG_nn'
Cuts at: 0 2222 2624 2687 3617 4890
Size: 2222 402 63 930 1273
Fragments arranged by size:
2222 1273 930 402 63
BsrFI r'CCGG_y
Cuts at: 0 32 689 1216 2407 4434 4890
Size: 32 657 527 1191 2027 456
Fragments arranged by size:
2027 1191 657 527 456 32
BssSI C'ACGA_G
Cuts at: 0 2380 4890
Size: 2380 2510
BstAPI GCAn_nnn'nTGC
Cuts at: 0 78 426 1116 1182 1893 3195 3226 3492
Size: 78 348 690 66 711 1302 31 266
Cuts at: 3492 4209 4890
Size: 717 681
Fragments arranged by size:
1302 717 711 690 681 348 266 78
66 31
BstEII G'GTnAC_C
Cuts at: 0 459 2466 4890
Size: 459 2007 2424
Fragments arranged by size:
2424 2007 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2973 3085 3100 4650 4890
Size: 2973 112 15 1550 240
Fragments arranged by size:
2973 1550 240 112 15
BstYI r'GATC_y
Cuts at: 0 89 209 287 537 1844 3011 4890
Size: 89 120 78 250 1307 1167 1879
Fragments arranged by size:
1879 1307 1167 250 120 89 78
Bsu36I CC'TnA_GG
Cuts at: 0 1766 2006 3698 3998 4890
Size: 1766 240 1692 300 892
Fragments arranged by size:
1766 1692 892 300 240
BtgI C'CryG_G
Cuts at: 0 1700 2090 2387 3127 3345 3422 4866 4890
Size: 1700 390 297 740 218 77 1444 24
Fragments arranged by size:
1700 1444 740 390 297 218 77 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1328 2808 4890
Size: 1328 1480 2082
Fragments arranged by size:
2082 1480 1328
BtsI GCAGTG_nn'
Cuts at: 0 424 631 1723 2411 2722 2924 3185 3540
Size: 424 207 1092 688 311 202 261 355
Cuts at: 3540 4890
Size: 1350
Fragments arranged by size:
1350 1092 688 424 355 311 261 207
202
DraIII CAC_nnn'GTG
Cuts at: 0 1633 4890
Size: 1633 3257
DrdI GACnn_nn'nnGTC
Cuts at: 0 2791 4890
Size: 2791 2099
EaeI y'GGCC_r
Cuts at: 0 233 1040 3137 3645 3812 3900 3974 4379
Size: 233 807 2097 508 167 88 74 405
Cuts at: 4379 4607 4890
Size: 228 283
Fragments arranged by size:
2097 807 508 405 283 233 228 167
88 74
EagI C'GGCC_G
Cuts at: 0 3974 4890
Size: 3974 916
EarI CTCTTCn'nnn_
Cuts at: 0 1447 1676 1988 2015 2042 2048 2168 2864
Size: 1447 229 312 27 27 6 120 696
Cuts at: 2864 3678 4189 4890
Size: 814 511 701
Fragments arranged by size:
1447 814 701 696 511 312 229 120
27 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1488 4890
Size: 1488 3402
EcoICRI GAG'CTC
Cuts at: 0 471 645 714 1440 4467 4890
Size: 471 174 69 726 3027 423
Fragments arranged by size:
3027 726 471 423 174 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 318 336 340 487 715 814 1329
Size: 133 185 18 4 147 228 99 515
Cuts at: 1329 1372 2066 2336 2362 2604 3268 3619 3682
Size: 43 694 270 26 242 664 351 63
Cuts at: 3682 3811 3875 3884 4108 4498 4618 4838 4890
Size: 129 64 9 224 390 120 220 52
Fragments arranged by size:
694 664 515 390 351 270 242 228
224 220 185 147 133 129 120 99
64 63 52 43 26 18 9 4
EcoO109I rG'GnC_Cy
Cuts at: 0 1149 1356 1404 1611 1770 1911 1912 2208
Size: 1149 207 48 207 159 141 1 296
Cuts at: 2208 2299 2564 2853 2854 3033 3034 3132 3273
Size: 91 265 289 1 179 1 98 141
Cuts at: 3273 3702 3748 3965 4113 4114 4473 4565 4591
Size: 429 46 217 148 1 359 92 26
Cuts at: 4591 4592 4614 4721 4793 4813 4876 4890
Size: 1 22 107 72 20 63 14
Fragments arranged by size:
1149 429 359 296 289 265 217 207
207 179 159 148 141 141 107 98
92 91 72 63 48 46 26 22
20 14 1 1 1 1 1
EcoRV GAT'ATC
Cuts at: 0 279 4890
Size: 279 4611
FspI TGC'GCA
Cuts at: 0 184 382 4890
Size: 184 198 4508
Fragments arranged by size:
4508 198 184
HaeII r_GCGC'y
Cuts at: 0 27 663 979 1416 2372 2398 2580 3830
Size: 27 636 316 437 956 26 182 1250
Cuts at: 3830 4367 4398 4808 4866 4890
Size: 537 31 410 58 24
Fragments arranged by size:
1250 956 636 537 437 410 316 182
58 31 27 26 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1492 1524 1600 1632 4039 4071 4705 4737
Size: 1492 32 76 32 2407 32 634 32
Cuts at: 4737 4890
Size: 153
Fragments arranged by size:
2407 1492 634 153 76 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 659 2368 4363 4394 4862 4890
Size: 659 1709 1995 31 468 28
Fragments arranged by size:
1995 1709 659 468 31 28
KpnI G_GTAC'C
Cuts at: 0 3850 4890
Size: 3850 1040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 2816 2966 3036 3089 3452 4890
Size: 662 2154 150 70 53 363 1438
Fragments arranged by size:
2154 1438 662 363 150 70 53
MscI TGG'CCA
Cuts at: 0 235 3902 4890
Size: 235 3667 988
Fragments arranged by size:
3667 988 235
MslI CAynn'nnrTG
Cuts at: 0 2416 4648 4890
Size: 2416 2232 242
Fragments arranged by size:
2416 2232 242
MspA1I CmG'CkG
Cuts at: 0 18 141 216 249 338 378 534 624
Size: 18 123 75 33 89 40 156 90
Cuts at: 624 792 1393 1800 2481 2809 2899 3129 3149
Size: 168 601 407 681 328 90 230 20
Cuts at: 3149 3347 3525 3862 4053 4890
Size: 198 178 337 191 837
Fragments arranged by size:
837 681 601 407 337 328 230 198
191 178 168 156 123 90 90 89
75 40 33 20 18
NaeI GCC'GGC
Cuts at: 0 2409 4890
Size: 2409 2481
NarI GG'CG_CC
Cuts at: 0 660 2369 4364 4395 4863 4890
Size: 660 1709 1995 31 468 27
Fragments arranged by size:
1995 1709 660 468 31 27
NgoMIV G'CCGG_C
Cuts at: 0 2407 4890
Size: 2407 2483
NspI r_CATG'y
Cuts at: 0 1896 4890
Size: 1896 2994
PasI CC'CwG_GG
Cuts at: 0 921 1955 4301 4353 4890
Size: 921 1034 2346 52 537
Fragments arranged by size:
2346 1034 921 537 52
PflMI CCAn_nnn'nTGG
Cuts at: 0 1350 1504 3028 4890
Size: 1350 154 1524 1862
Fragments arranged by size:
1862 1524 1350 154
PfoI T'CCnGG_A
Cuts at: 0 268 290 473 1349 2339 3092 4061 4890
Size: 268 22 183 876 990 753 969 829
Fragments arranged by size:
990 969 876 829 753 268 183 22
PmlI CAC'GTG
Cuts at: 0 39 1083 2583 4890
Size: 39 1044 1500 2307
Fragments arranged by size:
2307 1500 1044 39
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 4890
Size: 334 32 4524
Fragments arranged by size:
4524 334 32
PpuMI rG'GwC_Cy
Cuts at: 0 3273 4565 4721 4890
Size: 3273 1292 156 169
Fragments arranged by size:
3273 1292 169 156
PshAI GACnn'nnGTC
Cuts at: 0 4692 4890
Size: 4692 198
PspOMI G'GGCC_C
Cuts at: 0 1611 1911 1967 2563 2853 3033 3702 3748
Size: 1611 300 56 596 290 180 669 46
Cuts at: 3748 4113 4472 4591 4812 4875 4890
Size: 365 359 119 221 63 15
Fragments arranged by size:
1611 669 596 365 359 300 290 221
180 119 63 56 46 15
PspXI vC'TCGA_Gb
Cuts at: 0 970 1270 4890
Size: 970 300 3620
Fragments arranged by size:
3620 970 300
PstI C_TGCA'G
Cuts at: 0 74 422 1112 1392 1524 1889 2366 2729
Size: 74 348 690 280 132 365 477 363
Cuts at: 2729 3785 4038 4890
Size: 1056 253 852
Fragments arranged by size:
1056 852 690 477 365 363 348 280
253 132 74
PvuII CAG'CTG
Cuts at: 0 141 249 378 534 624 792 1393 3525
Size: 141 108 129 156 90 168 601 2132
Cuts at: 3525 3862 4053 4890
Size: 337 191 837
Fragments arranged by size:
2132 837 601 337 191 168 156 141
129 108 90
RsrII CG'GwC_CG
Cuts at: 0 1214 4890
Size: 1214 3676
SacI G_AGCT'C
Cuts at: 0 473 647 716 1442 4469 4890
Size: 473 174 69 726 3027 421
Fragments arranged by size:
3027 726 473 421 174 69
SacII CC_GC'GG
Cuts at: 0 3130 3348 4890
Size: 3130 218 1542
Fragments arranged by size:
3130 1542 218
SanDI GG'GwC_CC
Cuts at: 0 3273 4890
Size: 3273 1617
SapI GCTCTTCn'nnn_
Cuts at: 0 2168 4890
Size: 2168 2722
ScaI AGT'ACT
Cuts at: 0 4078 4890
Size: 4078 812
SexAI A'CCwGG_T
Cuts at: 0 455 4890
Size: 455 4435
SfcI C'TryA_G
Cuts at: 0 70 99 418 1108 1388 1408 1520 1885
Size: 70 29 319 690 280 20 112 365
Cuts at: 1885 2362 2652 2725 3781 3960 4034 4186 4890
Size: 477 290 73 1056 179 74 152 704
Fragments arranged by size:
1056 704 690 477 365 319 290 280
179 152 112 74 73 70 29 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3964 3973 4480 4890
Size: 3964 9 507 410
Fragments arranged by size:
3964 507 410 9
SfoI GGC'GCC
Cuts at: 0 661 2370 4365 4396 4864 4890
Size: 661 1709 1995 31 468 26
Fragments arranged by size:
1995 1709 661 468 31 26
SmaI CCC'GGG
Cuts at: 0 432 1734 1926 2311 2562 2937 3287 4685
Size: 432 1302 192 385 251 375 350 1398
Cuts at: 4685 4890
Size: 205
Fragments arranged by size:
1398 1302 432 385 375 350 251 205
192
SmlI C'TyrA_G
Cuts at: 0 4 487 970 1023 1144 1270 1441 4323
Size: 4 483 483 53 121 126 171 2882
Cuts at: 4323 4890
Size: 567
Fragments arranged by size:
2882 567 483 483 171 126 121 53
4
SphI G_CATG'C
Cuts at: 0 1896 4890
Size: 1896 2994
SrfI GCCC'GGGC
Cuts at: 0 2311 4890
Size: 2311 2579
StuI AGG'CCT
Cuts at: 0 1483 3958 4857 4890
Size: 1483 2475 899 33
Fragments arranged by size:
2475 1483 899 33
StyI C'CwwG_G
Cuts at: 0 3116 3410 4659 4890
Size: 3116 294 1249 231
Fragments arranged by size:
3116 1249 294 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 1147 2317 4129 4890
Size: 775 372 1170 1812 761
Fragments arranged by size:
1812 1170 775 761 372
TatI w'GTAC_w
Cuts at: 0 4076 4890
Size: 4076 814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 998 1030 2309 2341 2750 2779 2782 2811
Size: 998 32 1279 32 409 29 3 29
Cuts at: 2811 4538 4570 4890
Size: 1727 32 320
Fragments arranged by size:
1727 1279 998 409 320 32 32 32
29 29 3
Tth111I GACn'n_nGTC
Cuts at: 0 2740 4890
Size: 2740 2150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 951 1501 3316 4890
Size: 951 550 1815 1574
Fragments arranged by size:
1815 1574 951 550
XhoI C'TCGA_G
Cuts at: 0 970 1270 4890
Size: 970 300 3620
Fragments arranged by size:
3620 970 300
XmaI C'CCGG_G
Cuts at: 0 430 1732 1924 2309 2560 2935 3285 4683
Size: 430 1302 192 385 251 375 350 1398
Cuts at: 4683 4890
Size: 207
Fragments arranged by size:
1398 1302 430 385 375 350 251 207
192
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI AseI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FspI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII RsrII SacI SacII SanDI SapI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI ApaLI ApoI AscI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BmtI BsaBI
BsiWI BsmI BspHI BsrGI BssHII BstBI BstZ17I ClaI
CspCI DraI EcoNI EcoRI FalI FseI FspAI HincII
HindIII HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PsiI PsrI PvuI
SalI SbfI SgfI SgrAI SnaBI SpeI SspI SwaI
XbaI XmnI ZraI