Restriction map for
pF1KSDA0149
(Linear) (Six-base) MAPSORT of: KIAA0149.seq Check: 3837 from: 1 to: 2490
KIAA0149.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 1672 2490
Size: 721 951 818
Fragments arranged by size:
951 818 721
Acc65I G'GTAC_C
Cuts at: 0 1033 1783 2490
Size: 1033 750 707
Fragments arranged by size:
1033 750 707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2444 2490
Size: 2444 46
AfeI AGC'GCT
Cuts at: 0 982 2490
Size: 982 1508
AgeI A'CCGG_T
Cuts at: 0 1979 2490
Size: 1979 511
AleI CACnn'nnGTG
Cuts at: 0 86 791 2490
Size: 86 705 1699
Fragments arranged by size:
1699 705 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1300 2490
Size: 1300 1190
AlwNI CAG_nnn'CTG
Cuts at: 0 967 1246 1972 2011 2205 2383 2490
Size: 967 279 726 39 194 178 107
Fragments arranged by size:
967 726 279 194 178 107 39
ApaI G_GGCC'C
Cuts at: 0 252 289 1007 1136 1341 2059 2490
Size: 252 37 718 129 205 718 431
Fragments arranged by size:
718 718 431 252 205 129 37
ApaLI G'TGCA_C
Cuts at: 0 708 2490
Size: 708 1782
AvaI C'yCGr_G
Cuts at: 0 41 270 1748 1859 1930 1957 2052 2490
Size: 41 229 1478 111 71 27 95 438
Fragments arranged by size:
1478 438 229 111 95 71 41 27
BamHI G'GATC_C
Cuts at: 0 2098 2406 2490
Size: 2098 308 84
Fragments arranged by size:
2098 308 84
BanI G'GyrC_C
Cuts at: 0 529 560 898 1033 1783 2347 2490
Size: 529 31 338 135 750 564 143
Fragments arranged by size:
750 564 529 338 143 135 31
BanII G_rGCy'C
Cuts at: 0 252 289 381 1007 1136 1341 1435 1826
Size: 252 37 92 626 129 205 94 391
Cuts at: 1826 2053 2059 2260 2370 2490
Size: 227 6 201 110 120
Fragments arranged by size:
626 391 252 227 205 201 129 120
110 94 92 37 6
BbeI G_GCGC'C
Cuts at: 0 533 564 2490
Size: 533 31 1926
Fragments arranged by size:
1926 533 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 1022 1052 2115 2490
Size: 1022 30 1063 375
Fragments arranged by size:
1063 1022 375 30
BbvCI CC'TCA_GC
Cuts at: 0 1456 2490
Size: 1456 1034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2058 2092 2490
Size: 2058 34 398
Fragments arranged by size:
2058 398 34
BglI GCCn_nnn'nGGC
Cuts at: 0 635 722 761 896 1241 1549 1675 1851
Size: 635 87 39 135 345 308 126 176
Cuts at: 1851 2490
Size: 639
Fragments arranged by size:
639 635 345 308 176 135 126 87
39
BlpI GC'TnA_GC
Cuts at: 0 110 2012 2490
Size: 110 1902 478
Fragments arranged by size:
1902 478 110
Bme1580I G_kGCm'C
Cuts at: 0 252 289 712 931 1007 1136 1341 2059
Size: 252 37 423 219 76 129 205 718
Cuts at: 2059 2490
Size: 431
Fragments arranged by size:
718 431 423 252 219 205 129 76
37
BmgBI CAC'GTC
Cuts at: 0 84 2490
Size: 84 2406
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 867 1137 2490
Size: 291 576 270 1353
Fragments arranged by size:
1353 576 291 270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 627 837 1554 1725 2397 2490
Size: 627 210 717 171 672 93
Fragments arranged by size:
717 672 627 210 171 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 809 1607 1722 1812 2457 2490
Size: 153 656 798 115 90 645 33
Fragments arranged by size:
798 656 645 153 115 90 33
Bpu10I CC'TnA_GC
Cuts at: 0 1456 1815 2186 2490
Size: 1456 359 371 304
Fragments arranged by size:
1456 371 359 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 2490
Size: 1339 1151
BsaI GGTCTCn'nnnn_
Cuts at: 0 1773 2490
Size: 1773 717
BsaAI yAC'GTr
Cuts at: 0 473 2019 2490
Size: 473 1546 471
Fragments arranged by size:
1546 473 471
BsaHI Gr'CG_yC
Cuts at: 0 182 530 561 1706 2490
Size: 182 348 31 1145 784
Fragments arranged by size:
1145 784 348 182 31
BsaWI w'CCGG_w
Cuts at: 0 1979 2490
Size: 1979 511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 1370 1400 2490
Size: 244 30 1096 30 1090
Fragments arranged by size:
1096 1090 244 30 30
BseYI C'CCAG_C
Cuts at: 0 6 371 999 1135 1155 1334 1547 2278
Size: 6 365 628 136 20 179 213 731
Cuts at: 2278 2337 2490
Size: 59 153
Fragments arranged by size:
731 628 365 213 179 153 136 59
20 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 2490
Size: 796 1694
BsiEI CG_ry'CG
Cuts at: 0 369 434 618 634 2490
Size: 369 65 184 16 1856
Fragments arranged by size:
1856 369 184 65 16
BsiHKAI G_wGCw'C
Cuts at: 0 712 823 1826 2018 2053 2490
Size: 712 111 1003 192 35 437
Fragments arranged by size:
1003 712 437 192 111 35
BsmI GAATG_Cn'
Cuts at: 0 157 658 1201 2490
Size: 157 501 543 1289
Fragments arranged by size:
1289 543 501 157
Bsp1286I G_dGCh'C
Cuts at: 0 252 289 381 712 823 931 1007 1136
Size: 252 37 92 331 111 108 76 129
Cuts at: 1136 1341 1435 1826 2018 2053 2059 2260 2370
Size: 205 94 391 192 35 6 201 110
Cuts at: 2370 2490
Size: 120
Fragments arranged by size:
391 331 252 205 201 192 129 120
111 110 108 94 92 76 37 35
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 1069 1395 1672 2490
Size: 721 348 326 277 818
Fragments arranged by size:
818 721 348 326 277
BsrBI CCG'CTC
Cuts at: 0 1836 2117 2490
Size: 1836 281 373
Fragments arranged by size:
1836 373 281
BsrFI r'CCGG_y
Cuts at: 0 433 629 1120 1159 1543 1979 2490
Size: 433 196 491 39 384 436 511
Fragments arranged by size:
511 491 436 433 384 196 39
BssHII G'CGCG_C
Cuts at: 0 508 736 2490
Size: 508 228 1754
Fragments arranged by size:
1754 508 228
BstYI r'GATC_y
Cuts at: 0 2098 2406 2490
Size: 2098 308 84
Fragments arranged by size:
2098 308 84
Bsu36I CC'TnA_GG
Cuts at: 0 1700 1880 2490
Size: 1700 180 610
Fragments arranged by size:
1700 610 180
BtgI C'CryG_G
Cuts at: 0 341 411 500 524 588 729 768 1167
Size: 341 70 89 24 64 141 39 399
Cuts at: 1167 2094 2490
Size: 927 396
Fragments arranged by size:
927 399 396 341 141 89 70 64
39 24
BtsI GCAGTG_nn'
Cuts at: 0 124 252 444 670 784 931 933 1122
Size: 124 128 192 226 114 147 2 189
Cuts at: 1122 1259 2490
Size: 137 1231
Fragments arranged by size:
1231 226 192 189 147 137 128 124
114 2
DraIII CAC_nnn'GTG
Cuts at: 0 774 2490
Size: 774 1716
DrdI GACnn_nn'nnGTC
Cuts at: 0 54 1504 2490
Size: 54 1450 986
Fragments arranged by size:
1450 986 54
EaeI y'GGCC_r
Cuts at: 0 92 324 615 631 795 969 1759 1974
Size: 92 232 291 16 164 174 790 215
Cuts at: 1974 2250 2490
Size: 276 240
Fragments arranged by size:
790 291 276 240 232 215 174 164
92 16
EagI C'GGCC_G
Cuts at: 0 615 631 2490
Size: 615 16 1859
Fragments arranged by size:
1859 615 16
EarI CTCTTCn'nnn_
Cuts at: 0 1306 1892 2420 2490
Size: 1306 586 528 70
Fragments arranged by size:
1306 586 528 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2116 2173 2490
Size: 2116 57 317
Fragments arranged by size:
2116 317 57
EcoICRI GAG'CTC
Cuts at: 0 1824 2051 2490
Size: 1824 227 439
Fragments arranged by size:
1824 439 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 809 1607 1722 1812 2444 2457 2490
Size: 153 656 798 115 90 632 13 33
Fragments arranged by size:
798 656 632 153 115 90 33 13
EcoO109I rG'GnC_Cy
Cuts at: 0 248 285 286 646 869 1003 1082 1132
Size: 248 37 1 360 223 134 79 50
Cuts at: 1132 1229 1338 1506 1876 2056 2107 2202 2299
Size: 97 109 168 370 180 51 95 97
Cuts at: 2299 2490
Size: 191
Fragments arranged by size:
370 360 248 223 191 180 168 134
109 97 97 95 79 51 50 37
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2299 2490
Size: 2299 191
FspI TGC'GCA
Cuts at: 0 126 783 2490
Size: 126 657 1707
Fragments arranged by size:
1707 657 126
FspAI rTGC'GCAy
Cuts at: 0 783 2490
Size: 783 1707
HaeII r_GCGC'y
Cuts at: 0 420 533 564 695 984 2490
Size: 420 113 31 131 289 1506
Fragments arranged by size:
1506 420 289 131 113 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 1337 1369 1552 1567 1584 1599
Size: 1039 32 266 32 183 15 17 15
Cuts at: 1599 2490
Size: 891
Fragments arranged by size:
1039 891 266 183 32 32 17 15
15
KasI G'GCGC_C
Cuts at: 0 529 560 2490
Size: 529 31 1930
Fragments arranged by size:
1930 529 31
KpnI G_GTAC'C
Cuts at: 0 1037 1787 2490
Size: 1037 750 703
Fragments arranged by size:
1037 750 703
MscI TGG'CCA
Cuts at: 0 94 971 1976 2490
Size: 94 877 1005 514
Fragments arranged by size:
1005 877 514 94
MslI CAynn'nnrTG
Cuts at: 0 86 791 2490
Size: 86 705 1699
Fragments arranged by size:
1699 705 86
MspA1I CmG'CkG
Cuts at: 0 24 266 371 383 502 566 578 620
Size: 24 242 105 12 119 64 12 42
Cuts at: 620 686 731 791 800 839 1139 2096 2490
Size: 66 45 60 9 39 300 957 394
Fragments arranged by size:
957 394 300 242 119 105 66 64
60 45 42 39 24 12 12 9
NaeI GCC'GGC
Cuts at: 0 631 1122 1545 2490
Size: 631 491 423 945
Fragments arranged by size:
945 631 491 423
NarI GG'CG_CC
Cuts at: 0 530 561 2490
Size: 530 31 1929
Fragments arranged by size:
1929 530 31
NcoI C'CATG_G
Cuts at: 0 1167 2490
Size: 1167 1323
NgoMIV G'CCGG_C
Cuts at: 0 629 1120 1543 2490
Size: 629 491 423 947
Fragments arranged by size:
947 629 491 423
NspI r_CATG'y
Cuts at: 0 2079 2490
Size: 2079 411
PasI CC'CwG_GG
Cuts at: 0 1475 2490
Size: 1475 1015
PflMI CCAn_nnn'nTGG
Cuts at: 0 967 1246 2278 2383 2490
Size: 967 279 1032 105 107
Fragments arranged by size:
1032 967 279 107 105
PfoI T'CCnGG_A
Cuts at: 0 1390 2042 2490
Size: 1390 652 448
Fragments arranged by size:
1390 652 448
PmlI CAC'GTG
Cuts at: 0 473 2019 2490
Size: 473 1546 471
Fragments arranged by size:
1546 473 471
PpuMI rG'GwC_Cy
Cuts at: 0 646 869 1082 1229 1506 1876 2490
Size: 646 223 213 147 277 370 614
Fragments arranged by size:
646 614 370 277 223 213 147
PspOMI G'GGCC_C
Cuts at: 0 248 285 1003 1132 1337 2055 2490
Size: 248 37 718 129 205 718 435
Fragments arranged by size:
718 718 435 248 205 129 37
PstI C_TGCA'G
Cuts at: 0 119 259 571 691 1117 1406 1683 2490
Size: 119 140 312 120 426 289 277 807
Fragments arranged by size:
807 426 312 289 277 140 120 119
PvuII CAG'CTG
Cuts at: 0 791 839 1139 2490
Size: 791 48 300 1351
Fragments arranged by size:
1351 791 300 48
RsrII CG'GwC_CG
Cuts at: 0 2328 2490
Size: 2328 162
SacI G_AGCT'C
Cuts at: 0 1826 2053 2490
Size: 1826 227 437
Fragments arranged by size:
1826 437 227
SacII CC_GC'GG
Cuts at: 0 503 732 2097 2490
Size: 503 229 1365 393
Fragments arranged by size:
1365 503 393 229
SanDI GG'GwC_CC
Cuts at: 0 646 869 2490
Size: 646 223 1621
Fragments arranged by size:
1621 646 223
SapI GCTCTTCn'nnn_
Cuts at: 0 1306 2490
Size: 1306 1184
ScaI AGT'ACT
Cuts at: 0 280 2490
Size: 280 2210
SfcI C'TryA_G
Cuts at: 0 115 255 567 687 1113 1402 1679 2490
Size: 115 140 312 120 426 289 277 811
Fragments arranged by size:
811 426 312 289 277 140 120 115
SfoI GGC'GCC
Cuts at: 0 531 562 2490
Size: 531 31 1928
Fragments arranged by size:
1928 531 31
SgrAI Cr'CCGG_yG
Cuts at: 0 1979 2490
Size: 1979 511
SmaI CCC'GGG
Cuts at: 0 272 1861 1959 2490
Size: 272 1589 98 531
Fragments arranged by size:
1589 531 272 98
SmlI C'TyrA_G
Cuts at: 0 1354 2490
Size: 1354 1136
SrfI GCCC'GGGC
Cuts at: 0 272 2490
Size: 272 2218
StuI AGG'CCT
Cuts at: 0 952 2490
Size: 952 1538
StyI C'CwwG_G
Cuts at: 0 1167 2165 2490
Size: 1167 998 325
Fragments arranged by size:
1167 998 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1467 1755 2490
Size: 1467 288 735
Fragments arranged by size:
1467 735 288
TatI w'GTAC_w
Cuts at: 0 278 2490
Size: 278 2212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 1370 1402 2490
Size: 244 32 1094 32 1088
Fragments arranged by size:
1094 1088 244 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1108 2490
Size: 1108 1382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1222 1243 2301 2490
Size: 1222 21 1058 189
Fragments arranged by size:
1222 1058 189 21
XmaI C'CCGG_G
Cuts at: 0 270 1859 1957 2490
Size: 270 1589 98 533
Fragments arranged by size:
1589 533 270 98
Enzymes that do cut:
AarI Acc65I AcuI AfeI AgeI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BssHII BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
FspI FspAI HaeII Hin4I KasI KpnI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII RsrII SacI
SacII SanDI SapI ScaI SfcI SfoI SgrAI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BglII BmtI BsaBI BseRI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI EcoNI EcoRI EcoRV FseI
HincII HindIII HpaI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI SalI SbfI SexAI SfiI
SgfI SnaBI SpeI SphI SspI SwaI XbaI XhoI
XmnI ZraI