Restriction map for
pF1KSDA0150
(Linear) (Six-base) MAPSORT of: KIAA0150.seq Check: 8036 from: 1 to: 2844
KIAA0150.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1404 2030 2128 2844
Size: 1404 626 98 716
Fragments arranged by size:
1404 716 626 98
Acc65I G'GTAC_C
Cuts at: 0 94 2844
Size: 94 2750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1489 2218 2483 2844
Size: 1489 729 265 361
Fragments arranged by size:
1489 729 361 265
AfeI AGC'GCT
Cuts at: 0 2059 2844
Size: 2059 785
AflIII A'CryG_T
Cuts at: 0 2237 2243 2844
Size: 2237 6 601
Fragments arranged by size:
2237 601 6
AleI CACnn'nnGTG
Cuts at: 0 718 2844
Size: 718 2126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2718 2844
Size: 2718 126
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 2844
Size: 726 32 2086
Fragments arranged by size:
2086 726 32
AlwNI CAG_nnn'CTG
Cuts at: 0 48 109 1114 2222 2717 2844
Size: 48 61 1005 1108 495 127
Fragments arranged by size:
1108 1005 495 127 61 48
ApaI G_GGCC'C
Cuts at: 0 294 423 844 981 1542 1695 2444 2489
Size: 294 129 421 137 561 153 749 45
Cuts at: 2489 2787 2796 2844
Size: 298 9 48
Fragments arranged by size:
749 561 421 298 294 153 137 129
48 45 9
ApaLI G'TGCA_C
Cuts at: 0 2097 2844
Size: 2097 747
ApoI r'AATT_y
Cuts at: 0 442 2844
Size: 442 2402
AvaI C'yCGr_G
Cuts at: 0 240 293 476 500 519 635 875 962
Size: 240 53 183 24 19 116 240 87
Cuts at: 962 2080 2844
Size: 1118 764
Fragments arranged by size:
1118 764 240 240 183 116 87 53
24 19
AvrII C'CTAG_G
Cuts at: 0 2788 2844
Size: 2788 56
BamHI G'GATC_C
Cuts at: 0 1911 2844
Size: 1911 933
BanI G'GyrC_C
Cuts at: 0 94 1387 2116 2368 2667 2844
Size: 94 1293 729 252 299 177
Fragments arranged by size:
1293 729 299 252 177 94
BanII G_rGCy'C
Cuts at: 0 294 423 433 497 644 844 981 1076
Size: 294 129 10 64 147 200 137 95
Cuts at: 1076 1542 1695 1777 2444 2489 2761 2769 2787
Size: 466 153 82 667 45 272 8 18
Cuts at: 2787 2796 2844
Size: 9 48
Fragments arranged by size:
667 466 294 272 200 153 147 137
129 95 82 64 48 45 18 10
9 8
BbeI G_GCGC'C
Cuts at: 0 2372 2844
Size: 2372 472
BbsI GAAGACnn'nnnn_
Cuts at: 0 1480 1639 2844
Size: 1480 159 1205
Fragments arranged by size:
1480 1205 159
BbvCI CC'TCA_GC
Cuts at: 0 1420 2844
Size: 1420 1424
BclI T'GATC_A
Cuts at: 0 1604 2844
Size: 1604 1240
BglI GCCn_nnn'nGGC
Cuts at: 0 179 207 418 522 679 853 880 1686
Size: 179 28 211 104 157 174 27 806
Cuts at: 1686 1948 2791 2844
Size: 262 843 53
Fragments arranged by size:
843 806 262 211 179 174 157 104
53 28 27
Bme1580I G_kGCm'C
Cuts at: 0 156 294 423 844 981 1542 1695 2101
Size: 156 138 129 421 137 561 153 406
Cuts at: 2101 2119 2335 2444 2489 2787 2796 2844
Size: 18 216 109 45 298 9 48
Fragments arranged by size:
561 421 406 298 216 156 153 138
137 129 109 48 45 18 9
BmgBI CAC'GTC
Cuts at: 0 1097 2426 2839 2844
Size: 1097 1329 413 5
Fragments arranged by size:
1329 1097 413 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 1219 1862 2367 2844
Size: 1219 643 505 477
Fragments arranged by size:
1219 643 505 477
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1837 2844
Size: 1837 1007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1128 1391 2662 2844
Size: 480 648 263 1271 182
Fragments arranged by size:
1271 648 480 263 182
Bpu10I CC'TnA_GC
Cuts at: 0 1420 2691 2844
Size: 1420 1271 153
Fragments arranged by size:
1420 1271 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 2844
Size: 352 2492
BsaI GGTCTCn'nnnn_
Cuts at: 0 1291 2475 2844
Size: 1291 1184 369
Fragments arranged by size:
1291 1184 369
BsaAI yAC'GTr
Cuts at: 0 2238 2244 2844
Size: 2238 6 600
Fragments arranged by size:
2238 600 6
BsaHI Gr'CG_yC
Cuts at: 0 1636 2369 2527 2844
Size: 1636 733 158 317
Fragments arranged by size:
1636 733 317 158
BsaWI w'CCGG_w
Cuts at: 0 917 1343 2844
Size: 917 426 1501
Fragments arranged by size:
1501 917 426
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1195 1225 1831 1861 2660 2690 2844
Size: 1195 30 606 30 799 30 154
Fragments arranged by size:
1195 799 606 154 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 939 1149 1152 2610 2613 2616 2619 2622
Size: 939 210 3 1458 3 3 3 3
Cuts at: 2622 2625 2844
Size: 3 219
Fragments arranged by size:
1458 939 219 210 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 99 422 738 1120 1214 2443 2507 2530
Size: 99 323 316 382 94 1229 64 23
Cuts at: 2530 2768 2844
Size: 238 76
Fragments arranged by size:
1229 382 323 316 238 99 94 76
64 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1571 1975 2549 2809 2844
Size: 1571 404 574 260 35
Fragments arranged by size:
1571 574 404 260 35
BsiEI CG_ry'CG
Cuts at: 0 280 2844
Size: 280 2564
BsiHKAI G_wGCw'C
Cuts at: 0 768 2101 2188 2707 2844
Size: 768 1333 87 519 137
Fragments arranged by size:
1333 768 519 137 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1341 2844
Size: 1341 1503
Bsp1286I G_dGCh'C
Cuts at: 0 156 294 423 433 497 644 768 844
Size: 156 138 129 10 64 147 124 76
Cuts at: 844 981 1076 1542 1695 1777 2101 2119 2188
Size: 137 95 466 153 82 324 18 69
Cuts at: 2188 2335 2444 2489 2707 2761 2769 2787 2796
Size: 147 109 45 218 54 8 18 9
Cuts at: 2796 2844
Size: 48
Fragments arranged by size:
466 324 218 156 153 147 147 138
137 129 124 109 95 82 76 69
64 54 48 45 18 18 10 9
8
BspEI T'CCGG_A
Cuts at: 0 917 1343 2844
Size: 917 426 1501
Fragments arranged by size:
1501 917 426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 553 1404 2030 2128 2149 2844
Size: 553 851 626 98 21 695
Fragments arranged by size:
851 695 626 553 98 21
BsrBI CCG'CTC
Cuts at: 0 700 1305 2844
Size: 700 605 1539
Fragments arranged by size:
1539 700 605
BsrDI GCAATG_nn'
Cuts at: 0 84 2844
Size: 84 2760
BsrFI r'CCGG_y
Cuts at: 0 852 1192 1638 1904 2178 2844
Size: 852 340 446 266 274 666
Fragments arranged by size:
852 666 446 340 274 266
BssSI C'ACGA_G
Cuts at: 0 226 2662 2844
Size: 226 2436 182
Fragments arranged by size:
2436 226 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1578 2844
Size: 1578 1266
BstEII G'GTnAC_C
Cuts at: 0 1500 2844
Size: 1500 1344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1719 2844
Size: 1719 1125
BstYI r'GATC_y
Cuts at: 0 29 558 1911 2844
Size: 29 529 1353 933
Fragments arranged by size:
1353 933 529 29
Bsu36I CC'TnA_GG
Cuts at: 0 414 858 1444 2844
Size: 414 444 586 1400
Fragments arranged by size:
1400 586 444 414
BtgI C'CryG_G
Cuts at: 0 72 195 752 2049 2364 2481 2558 2844
Size: 72 123 557 1297 315 117 77 286
Fragments arranged by size:
1297 557 315 286 123 117 77 72
BtsI GCAGTG_nn'
Cuts at: 0 555 624 1958 2546 2574 2844
Size: 555 69 1334 588 28 270
Fragments arranged by size:
1334 588 555 270 69 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1022 1057 2844
Size: 1022 35 1787
Fragments arranged by size:
1787 1022 35
DraIII CAC_nnn'GTG
Cuts at: 0 2160 2844
Size: 2160 684
EaeI y'GGCC_r
Cuts at: 0 277 755 801 1860 1940 1967 2090 2474
Size: 277 478 46 1059 80 27 123 384
Cuts at: 2474 2844
Size: 370
Fragments arranged by size:
1059 478 384 370 277 123 80 46
27
EagI C'GGCC_G
Cuts at: 0 277 2844
Size: 277 2567
EarI CTCTTCn'nnn_
Cuts at: 0 192 569 949 1170 1318 1447 1996 2844
Size: 192 377 380 221 148 129 549 848
Fragments arranged by size:
848 549 380 377 221 192 148 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1446 1803 2844
Size: 22 1424 357 1041
Fragments arranged by size:
1424 1041 357 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1128 1391 1489 2218 2483 2662 2844
Size: 480 648 263 98 729 265 179 182
Fragments arranged by size:
729 648 480 265 263 182 179 98
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 1495 2803 2844
Size: 40 1455 1308 41
Fragments arranged by size:
1455 1308 41 40
EcoO109I rG'GnC_Cy
Cuts at: 0 244 290 419 473 542 840 841 862
Size: 244 46 129 54 69 298 1 21
Cuts at: 862 977 978 1238 1440 1488 1715 1818 1886
Size: 115 1 260 202 48 227 103 68
Cuts at: 1886 2138 2440 2784 2792 2793 2844
Size: 252 302 344 8 1 51
Fragments arranged by size:
344 302 298 260 252 244 227 202
129 115 103 69 68 54 51 48
46 21 8 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 580 702 2844
Size: 580 122 2142
Fragments arranged by size:
2142 580 122
FspI TGC'GCA
Cuts at: 0 1897 2844
Size: 1897 947
HaeII r_GCGC'y
Cuts at: 0 1658 2061 2372 2514 2844
Size: 1658 403 311 142 330
Fragments arranged by size:
1658 403 330 311 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1195 1227 1427 1459 2656 2688 2844
Size: 1195 32 200 32 1197 32 156
Fragments arranged by size:
1197 1195 200 156 32 32 32
KasI G'GCGC_C
Cuts at: 0 2368 2844
Size: 2368 476
KpnI G_GTAC'C
Cuts at: 0 98 2844
Size: 98 2746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 2732 2844
Size: 1166 1566 112
Fragments arranged by size:
1566 1166 112
MscI TGG'CCA
Cuts at: 0 757 803 1942 1969 2844
Size: 757 46 1139 27 875
Fragments arranged by size:
1139 875 757 46 27
MslI CAynn'nnrTG
Cuts at: 0 77 718 2844
Size: 77 641 2126
Fragments arranged by size:
2126 641 77
MspA1I CmG'CkG
Cuts at: 0 426 510 761 1751 1805 1925 2366 2382
Size: 426 84 251 990 54 120 441 16
Cuts at: 2382 2844
Size: 462
Fragments arranged by size:
990 462 441 426 251 120 84 54
16
NaeI GCC'GGC
Cuts at: 0 1194 1640 1906 2844
Size: 1194 446 266 938
Fragments arranged by size:
1194 938 446 266
NarI GG'CG_CC
Cuts at: 0 2369 2844
Size: 2369 475
NcoI C'CATG_G
Cuts at: 0 195 2844
Size: 195 2649
NgoMIV G'CCGG_C
Cuts at: 0 1192 1638 1904 2844
Size: 1192 446 266 940
Fragments arranged by size:
1192 940 446 266
NspI r_CATG'y
Cuts at: 0 328 1576 2022 2844
Size: 328 1248 446 822
Fragments arranged by size:
1248 822 446 328
PasI CC'CwG_GG
Cuts at: 0 726 2295 2437 2581 2774 2844
Size: 726 1569 142 144 193 70
Fragments arranged by size:
1569 726 193 144 142 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 715 2844
Size: 715 2129
PfoI T'CCnGG_A
Cuts at: 0 1368 2678 2844
Size: 1368 1310 166
Fragments arranged by size:
1368 1310 166
PmlI CAC'GTG
Cuts at: 0 2238 2844
Size: 2238 606
PpuMI rG'GwC_Cy
Cuts at: 0 244 542 862 1238 1715 1818 2138 2844
Size: 244 298 320 376 477 103 320 706
Fragments arranged by size:
706 477 376 320 320 298 244 103
PspOMI G'GGCC_C
Cuts at: 0 290 419 840 977 1538 1691 2440 2485
Size: 290 129 421 137 561 153 749 45
Cuts at: 2485 2783 2792 2844
Size: 298 9 52
Fragments arranged by size:
749 561 421 298 290 153 137 129
52 45 9
PspXI vC'TCGA_Gb
Cuts at: 0 519 2844
Size: 519 2325
PstI C_TGCA'G
Cuts at: 0 44 515 550 1002 1920 2212 2269 2747
Size: 44 471 35 452 918 292 57 478
Cuts at: 2747 2844
Size: 97
Fragments arranged by size:
918 478 471 452 292 97 57 44
35
PvuII CAG'CTG
Cuts at: 0 510 761 1925 2382 2844
Size: 510 251 1164 457 462
Fragments arranged by size:
1164 510 462 457 251
SacII CC_GC'GG
Cuts at: 0 2367 2844
Size: 2367 477
SanDI GG'GwC_CC
Cuts at: 0 244 2844
Size: 244 2600
SapI GCTCTTCn'nnn_
Cuts at: 0 1318 1447 2844
Size: 1318 129 1397
Fragments arranged by size:
1397 1318 129
SbfI CC_TGCA'GG
Cuts at: 0 1920 2844
Size: 1920 924
ScaI AGT'ACT
Cuts at: 0 2014 2844
Size: 2014 830
SexAI A'CCwGG_T
Cuts at: 0 2101 2844
Size: 2101 743
SfcI C'TryA_G
Cuts at: 0 40 511 546 998 1916 1926 2208 2229
Size: 40 471 35 452 918 10 282 21
Cuts at: 2229 2265 2743 2844
Size: 36 478 101
Fragments arranged by size:
918 478 471 452 282 101 40 36
35 21 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 880 2791 2844
Size: 880 1911 53
Fragments arranged by size:
1911 880 53
SfoI GGC'GCC
Cuts at: 0 2370 2844
Size: 2370 474
SmaI CCC'GGG
Cuts at: 0 242 295 478 637 877 964 2844
Size: 242 53 183 159 240 87 1880
Fragments arranged by size:
1880 242 240 183 159 87 53
SmlI C'TyrA_G
Cuts at: 0 331 519 2844
Size: 331 188 2325
Fragments arranged by size:
2325 331 188
SphI G_CATG'C
Cuts at: 0 1576 2844
Size: 1576 1268
StuI AGG'CCT
Cuts at: 0 883 1018 1141 2844
Size: 883 135 123 1703
Fragments arranged by size:
1703 883 135 123
StyI C'CwwG_G
Cuts at: 0 195 777 1553 1780 2165 2654 2788 2804
Size: 195 582 776 227 385 489 134 16
Cuts at: 2804 2844
Size: 40
Fragments arranged by size:
776 582 489 385 227 195 134 40
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 138 927 1605 2287 2844
Size: 101 37 789 678 682 557
Fragments arranged by size:
789 682 678 557 101 37
TatI w'GTAC_w
Cuts at: 0 1127 2012 2021 2844
Size: 1127 885 9 823
Fragments arranged by size:
1127 885 823 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 534 566 642 674 1350 1382 2146 2178
Size: 534 32 76 32 676 32 764 32
Cuts at: 2178 2660 2692 2844
Size: 482 32 152
Fragments arranged by size:
764 676 534 482 152 76 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1517 2844
Size: 1517 1327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 583 1488 2844
Size: 583 905 1356
Fragments arranged by size:
1356 905 583
XhoI C'TCGA_G
Cuts at: 0 519 2844
Size: 519 2325
XmaI C'CCGG_G
Cuts at: 0 240 293 476 635 875 962 2844
Size: 240 53 183 159 240 87 1882
Fragments arranged by size:
1882 240 240 183 159 87 53
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacII
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AscI AseI
AsiSI BaeI BcgI BciVI BfrBI BglII BlpI BmtI
BsaBI BsiWI BsmI BspHI BsrGI BssHII BstBI BstZ17I
BtgZI ClaI DraI DrdI EcoICRI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SalI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI XmnI ZraI