Restriction map for
pF1KSDA0154
(Linear) (Six-base) MAPSORT of: KIAA0154.seq Check: 1454 from: 1 to: 2070
KIAA0154.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1163 1926 2070
Size: 1163 763 144
Fragments arranged by size:
1163 763 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1173 1978 2070
Size: 334 839 805 92
Fragments arranged by size:
839 805 334 92
AflIII A'CryG_T
Cuts at: 0 548 2070
Size: 548 1522
AhdI GACnn_n'nnGTC
Cuts at: 0 216 539 2070
Size: 216 323 1531
Fragments arranged by size:
1531 323 216
AleI CACnn'nnGTG
Cuts at: 0 2020 2070
Size: 2020 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1672 1846 2070
Size: 1672 174 224
Fragments arranged by size:
1672 224 174
AlwNI CAG_nnn'CTG
Cuts at: 0 195 1303 1402 1593 2070
Size: 195 1108 99 191 477
Fragments arranged by size:
1108 477 195 191 99
ApaI G_GGCC'C
Cuts at: 0 1040 1571 2070
Size: 1040 531 499
Fragments arranged by size:
1040 531 499
AvaI C'yCGr_G
Cuts at: 0 761 1488 2070
Size: 761 727 582
Fragments arranged by size:
761 727 582
BamHI G'GATC_C
Cuts at: 0 521 2070
Size: 521 1549
BanI G'GyrC_C
Cuts at: 0 1151 1219 1406 2070
Size: 1151 68 187 664
Fragments arranged by size:
1151 664 187 68
BanII G_rGCy'C
Cuts at: 0 1040 1342 1394 1571 1637 2070
Size: 1040 302 52 177 66 433
Fragments arranged by size:
1040 433 302 177 66 52
BbeI G_GCGC'C
Cuts at: 0 1223 2070
Size: 1223 847
BbsI GAAGACnn'nnnn_
Cuts at: 0 238 515 2070
Size: 238 277 1555
Fragments arranged by size:
1555 277 238
BbvCI CC'TCA_GC
Cuts at: 0 1248 2070
Size: 1248 822
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 895 2070
Size: 861 34 1175
Fragments arranged by size:
1175 861 34
BclI T'GATC_A
Cuts at: 0 99 657 2070
Size: 99 558 1413
Fragments arranged by size:
1413 558 99
BlpI GC'TnA_GC
Cuts at: 0 2011 2070
Size: 2011 59
Bme1580I G_kGCm'C
Cuts at: 0 1040 1409 1571 1661 1689 1847 2070
Size: 1040 369 162 90 28 158 223
Fragments arranged by size:
1040 369 223 162 158 90 28
BmgBI CAC'GTC
Cuts at: 0 1770 2070
Size: 1770 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 1325 2070
Size: 84 1241 745
Fragments arranged by size:
1241 745 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 172 1144 1225 1379 2070
Size: 46 126 972 81 154 691
Fragments arranged by size:
972 691 154 126 81 46
Bpu10I CC'TnA_GC
Cuts at: 0 1248 1631 2070
Size: 1248 383 439
Fragments arranged by size:
1248 439 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1245 2070
Size: 1245 825
BsaAI yAC'GTr
Cuts at: 0 1656 2070
Size: 1656 414
BsaHI Gr'CG_yC
Cuts at: 0 299 1220 2070
Size: 299 921 850
Fragments arranged by size:
921 850 299
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 1045 1075 2070
Size: 742 30 273 30 995
Fragments arranged by size:
995 742 273 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 939 999 1090 1223 1248 1332 2070
Size: 430 509 60 91 133 25 84 738
Fragments arranged by size:
738 509 430 133 91 84 60 25
BseYI C'CCAG_C
Cuts at: 0 930 1039 1104 1135 1296 1311 1368 1536
Size: 930 109 65 31 161 15 57 168
Cuts at: 1536 2070
Size: 534
Fragments arranged by size:
930 534 168 161 109 65 57 31
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 529 879 1423 2070
Size: 352 177 350 544 647
Fragments arranged by size:
647 544 352 350 177
BsiHKAI G_wGCw'C
Cuts at: 0 1637 2017 2070
Size: 1637 380 53
Fragments arranged by size:
1637 380 53
Bsp1286I G_dGCh'C
Cuts at: 0 1040 1342 1394 1409 1571 1637 1661 1689
Size: 1040 302 52 15 162 66 24 28
Cuts at: 1689 1847 2017 2070
Size: 158 170 53
Fragments arranged by size:
1040 302 170 162 158 66 53 52
28 24 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 789 1163 1926 2070
Size: 476 313 374 763 144
Fragments arranged by size:
763 476 374 313 144
BsrBI CCG'CTC
Cuts at: 0 1007 1581 2070
Size: 1007 574 489
Fragments arranged by size:
1007 574 489
BsrGI T'GTAC_A
Cuts at: 0 254 2070
Size: 254 1816
BssHII G'CGCG_C
Cuts at: 0 1433 2070
Size: 1433 637
BstEII G'GTnAC_C
Cuts at: 0 531 2070
Size: 531 1539
BstXI CCAn_nnnn'nTGG
Cuts at: 0 176 2070
Size: 176 1894
BstYI r'GATC_y
Cuts at: 0 158 521 2070
Size: 158 363 1549
Fragments arranged by size:
1549 363 158
Bsu36I CC'TnA_GG
Cuts at: 0 951 987 2070
Size: 951 36 1083
Fragments arranged by size:
1083 951 36
BtgI C'CryG_G
Cuts at: 0 266 497 997 1410 1562 2070
Size: 266 231 500 413 152 508
Fragments arranged by size:
508 500 413 266 231 152
BtsI GCAGTG_nn'
Cuts at: 0 1279 1689 1847 2070
Size: 1279 410 158 223
Fragments arranged by size:
1279 410 223 158
ClaI AT'CG_AT
Cuts at: 0 1669 2070
Size: 1669 401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 335 370 2070
Size: 335 35 1700
Fragments arranged by size:
1700 335 35
DraI TTT'AAA
Cuts at: 0 690 2070
Size: 690 1380
EaeI y'GGCC_r
Cuts at: 0 1643 1946 2070
Size: 1643 303 124
Fragments arranged by size:
1643 303 124
EarI CTCTTCn'nnn_
Cuts at: 0 310 624 1466 1588 1735 2070
Size: 310 314 842 122 147 335
Fragments arranged by size:
842 335 314 310 147 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 25 693 2070
Size: 19 6 668 1377
Fragments arranged by size:
1377 668 19 6
EcoICRI GAG'CTC
Cuts at: 0 1635 2070
Size: 1635 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 172 334 1144 1173 1225 1379 1978
Size: 46 126 162 810 29 52 154 599
Cuts at: 1978 2070
Size: 92
Fragments arranged by size:
810 599 162 154 126 92 52 46
29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 2070
Size: 472 1598
EcoO109I rG'GnC_Cy
Cuts at: 0 192 991 1036 1544 1567 1627 2070
Size: 192 799 45 508 23 60 443
Fragments arranged by size:
799 508 443 192 60 45 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2007 2070
Size: 2007 63
HaeII r_GCGC'y
Cuts at: 0 187 1223 2070
Size: 187 1036 847
Fragments arranged by size:
1036 847 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 360 392 406 438 525 557
Size: 202 32 126 32 14 32 87 32
Cuts at: 557 742 774 2070
Size: 185 32 1296
Fragments arranged by size:
1296 202 185 126 87 32 32 32
32 32 14
HincII GTy'rAC
Cuts at: 0 564 2070
Size: 564 1506
HindIII A'AGCT_T
Cuts at: 0 427 2070
Size: 427 1643
HpaI GTT'AAC
Cuts at: 0 564 2070
Size: 564 1506
KasI G'GCGC_C
Cuts at: 0 1219 2070
Size: 1219 851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 165 1199 1250 2070
Size: 83 82 1034 51 820
Fragments arranged by size:
1034 820 83 82 51
MscI TGG'CCA
Cuts at: 0 1645 1948 2070
Size: 1645 303 122
Fragments arranged by size:
1645 303 122
MslI CAynn'nnrTG
Cuts at: 0 174 1448 1654 2020 2070
Size: 174 1274 206 366 50
Fragments arranged by size:
1274 366 206 174 50
MspA1I CmG'CkG
Cuts at: 0 260 1135 1210 2010 2070
Size: 260 875 75 800 60
Fragments arranged by size:
875 800 260 75 60
NarI GG'CG_CC
Cuts at: 0 1220 2070
Size: 1220 850
NcoI C'CATG_G
Cuts at: 0 266 497 1410 2070
Size: 266 231 913 660
Fragments arranged by size:
913 660 266 231
NspI r_CATG'y
Cuts at: 0 312 2070
Size: 312 1758
PasI CC'CwG_GG
Cuts at: 0 1032 1402 1608 1998 2070
Size: 1032 370 206 390 72
Fragments arranged by size:
1032 390 370 206 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 1416 1879 2070
Size: 1416 463 191
Fragments arranged by size:
1416 463 191
PmlI CAC'GTG
Cuts at: 0 1656 2070
Size: 1656 414
PpuMI rG'GwC_Cy
Cuts at: 0 991 2070
Size: 991 1079
PspOMI G'GGCC_C
Cuts at: 0 1036 1567 2070
Size: 1036 531 503
Fragments arranged by size:
1036 531 503
PstI C_TGCA'G
Cuts at: 0 473 1835 1842 1871 1923 2070
Size: 473 1362 7 29 52 147
Fragments arranged by size:
1362 473 147 52 29 7
PvuII CAG'CTG
Cuts at: 0 260 1135 2010 2070
Size: 260 875 875 60
Fragments arranged by size:
875 875 260 60
SacI G_AGCT'C
Cuts at: 0 1637 2070
Size: 1637 433
SapI GCTCTTCn'nnn_
Cuts at: 0 1588 2070
Size: 1588 482
SbfI CC_TGCA'GG
Cuts at: 0 473 2070
Size: 473 1597
SfcI C'TryA_G
Cuts at: 0 469 1831 1838 1867 1919 2070
Size: 469 1362 7 29 52 151
Fragments arranged by size:
1362 469 151 52 29 7
SfoI GGC'GCC
Cuts at: 0 1221 2070
Size: 1221 849
SmaI CCC'GGG
Cuts at: 0 763 2070
Size: 763 1307
SmlI C'TyrA_G
Cuts at: 0 1260 2070
Size: 1260 810
StuI AGG'CCT
Cuts at: 0 985 2070
Size: 985 1085
StyI C'CwwG_G
Cuts at: 0 236 266 497 867 1373 1410 1742 2070
Size: 236 30 231 370 506 37 332 328
Fragments arranged by size:
506 370 332 328 236 231 37 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1945 2070
Size: 1945 125
TatI w'GTAC_w
Cuts at: 0 254 2070
Size: 254 1816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1583 1615 2070
Size: 1583 32 455
Fragments arranged by size:
1583 455 32
XbaI T'CTAG_A
Cuts at: 0 1467 2070
Size: 1467 603
XmaI C'CCGG_G
Cuts at: 0 761 2070
Size: 761 1309
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrGI BssHII BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI CspCI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII HpaI KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PasI PflMI PmlI PpuMI PspOMI PstI
PvuII SacI SapI SbfI SfcI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BfrBI BglI BglII BmtI BplI BsaI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BssSI BstAPI BstBI BstZ17I BtgZI DraIII DrdI EagI
EcoRI EcoRV FseI FspI FspAI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XcmI XhoI XmnI ZraI