Restriction map for
pF1KSDA0161
(Linear) (Six-base) MAPSORT of: KIAA0161.seq Check: 2144 from: 1 to: 876
KIAA0161.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 352 442 876
Size: 352 90 434
Fragments arranged by size:
434 352 90
AccI GT'mk_AC
Cuts at: 0 569 876
Size: 569 307
Acc65I G'GTAC_C
Cuts at: 0 14 876
Size: 14 862
AfeI AGC'GCT
Cuts at: 0 550 876
Size: 550 326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 207 876
Size: 207 669
AlwNI CAG_nnn'CTG
Cuts at: 0 202 876
Size: 202 674
ApoI r'AATT_y
Cuts at: 0 421 876
Size: 421 455
AvaI C'yCGr_G
Cuts at: 0 496 716 876
Size: 496 220 160
Fragments arranged by size:
496 220 160
BanI G'GyrC_C
Cuts at: 0 14 329 449 876
Size: 14 315 120 427
Fragments arranged by size:
427 315 120 14
BanII G_rGCy'C
Cuts at: 0 158 876
Size: 158 718
BclI T'GATC_A
Cuts at: 0 161 876
Size: 161 715
BfrBI ATG'CAT
Cuts at: 0 119 876
Size: 119 757
Bme1580I G_kGCm'C
Cuts at: 0 334 876
Size: 334 542
BmgBI CAC'GTC
Cuts at: 0 372 876
Size: 372 504
BmrI ACTGGGnnnn_n'
Cuts at: 0 76 102 876
Size: 76 26 774
Fragments arranged by size:
774 76 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 664 876
Size: 349 315 212
Fragments arranged by size:
349 315 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 466 496 876
Size: 466 30 380
Fragments arranged by size:
466 380 30
BsaHI Gr'CG_yC
Cuts at: 0 337 876
Size: 337 539
BseYI C'CCAG_C
Cuts at: 0 389 705 876
Size: 389 316 171
Fragments arranged by size:
389 316 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 864 876
Size: 418 446 12
Fragments arranged by size:
446 418 12
BsiHKAI G_wGCw'C
Cuts at: 0 158 876
Size: 158 718
BsmBI CGTCTCn'nnnn_
Cuts at: 0 575 876
Size: 575 301
Bsp1286I G_dGCh'C
Cuts at: 0 158 334 876
Size: 158 176 542
Fragments arranged by size:
542 176 158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 352 442 876
Size: 352 90 434
Fragments arranged by size:
434 352 90
BsrFI r'CCGG_y
Cuts at: 0 17 876
Size: 17 859
BssHII G'CGCG_C
Cuts at: 0 593 876
Size: 593 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 410 614 876
Size: 410 204 262
Fragments arranged by size:
410 262 204
BtsI GCAGTG_nn'
Cuts at: 0 406 876
Size: 406 470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 801 836 876
Size: 801 35 40
Fragments arranged by size:
801 40 35
EaeI y'GGCC_r
Cuts at: 0 456 809 876
Size: 456 353 67
Fragments arranged by size:
456 353 67
EcoICRI GAG'CTC
Cuts at: 0 156 876
Size: 156 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 664 876
Size: 349 315 212
Fragments arranged by size:
349 315 212
EcoO109I rG'GnC_Cy
Cuts at: 0 30 688 876
Size: 30 658 188
Fragments arranged by size:
658 188 30
EcoRI G'AATT_C
Cuts at: 0 421 876
Size: 421 455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 642 876
Size: 642 234
HaeII r_GCGC'y
Cuts at: 0 552 876
Size: 552 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 720 752 876
Size: 720 32 124
Fragments arranged by size:
720 124 32
KpnI G_GTAC'C
Cuts at: 0 18 876
Size: 18 858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 876
Size: 301 575
MscI TGG'CCA
Cuts at: 0 458 811 876
Size: 458 353 65
Fragments arranged by size:
458 353 65
MspA1I CmG'CkG
Cuts at: 0 48 259 446 876
Size: 48 211 187 430
Fragments arranged by size:
430 211 187 48
NsiI A_TGCA'T
Cuts at: 0 121 876
Size: 121 755
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 876
Size: 372 504
PfoI T'CCnGG_A
Cuts at: 0 365 876
Size: 365 511
PpuMI rG'GwC_Cy
Cuts at: 0 30 688 876
Size: 30 658 188
Fragments arranged by size:
658 188 30
PstI C_TGCA'G
Cuts at: 0 383 876
Size: 383 493
PvuII CAG'CTG
Cuts at: 0 259 446 876
Size: 259 187 430
Fragments arranged by size:
430 259 187
SacI G_AGCT'C
Cuts at: 0 158 876
Size: 158 718
SanDI GG'GwC_CC
Cuts at: 0 688 876
Size: 688 188
SfcI C'TryA_G
Cuts at: 0 226 379 876
Size: 226 153 497
Fragments arranged by size:
497 226 153
SmaI CCC'GGG
Cuts at: 0 498 718 876
Size: 498 220 158
Fragments arranged by size:
498 220 158
TatI w'GTAC_w
Cuts at: 0 127 822 876
Size: 127 695 54
Fragments arranged by size:
695 127 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 876
Size: 30 846
XmaI C'CCGG_G
Cuts at: 0 496 716 876
Size: 496 220 160
Fragments arranged by size:
496 220 160
Enzymes that do cut:
AarI AccI Acc65I AfeI AlfI AlwNI ApoI AvaI
BanI BanII BclI BfrBI Bme1580I BmgBI BmrI BpmI
BsaI BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrFI BssHII BstAPI BtsI CspCI EaeI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I KpnI MmeI MscI
MspA1I NsiI PflMI PfoI PpuMI PstI PvuII SacI
SanDI SfcI SmaI TatI XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BglI BglII
BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI