Restriction map for
pF1KSDA0163
(Linear) (Six-base) MAPSORT of: KIAA0163.seq Check: 6044 from: 1 to: 1650
KIAA0163.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 898 1650
Size: 898 752
AccI GT'mk_AC
Cuts at: 0 392 1650
Size: 392 1258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 614 691 1650
Size: 306 308 77 959
Fragments arranged by size:
959 308 306 77
AflIII A'CryG_T
Cuts at: 0 689 808 1650
Size: 689 119 842
Fragments arranged by size:
842 689 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1564 1650
Size: 1564 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 829 861 1363 1395 1650
Size: 829 32 502 32 255
Fragments arranged by size:
829 502 255 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 441 480 1438 1488 1650
Size: 441 39 958 50 162
Fragments arranged by size:
958 441 162 50 39
AvaI C'yCGr_G
Cuts at: 0 514 1650
Size: 514 1136
AvrII C'CTAG_G
Cuts at: 0 333 1650
Size: 333 1317
BamHI G'GATC_C
Cuts at: 0 246 1184 1650
Size: 246 938 466
Fragments arranged by size:
938 466 246
BanI G'GyrC_C
Cuts at: 0 3 22 118 356 734 1121 1472 1650
Size: 3 19 96 238 378 387 351 178
Fragments arranged by size:
387 378 351 238 178 96 19 3
BbeI G_GCGC'C
Cuts at: 0 26 1125 1476 1650
Size: 26 1099 351 174
Fragments arranged by size:
1099 351 174 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 1529 1650
Size: 773 756 121
Fragments arranged by size:
773 756 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 332 1650
Size: 298 34 1318
Fragments arranged by size:
1318 298 34
BglI GCCn_nnn'nGGC
Cuts at: 0 72 446 1510 1650
Size: 72 374 1064 140
Fragments arranged by size:
1064 374 140 72
Bme1580I G_kGCm'C
Cuts at: 0 8 346 702 1559 1650
Size: 8 338 356 857 91
Fragments arranged by size:
857 356 338 91 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 423 1650
Size: 423 1227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1650
Size: 1498 152
Bpu10I CC'TnA_GC
Cuts at: 0 397 880 1650
Size: 397 483 770
Fragments arranged by size:
770 483 397
BsaHI Gr'CG_yC
Cuts at: 0 23 1122 1473 1650
Size: 23 1099 351 177
Fragments arranged by size:
1099 351 177 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 513 543 1342 1372 1443 1473 1650
Size: 513 30 799 30 71 30 177
Fragments arranged by size:
799 513 177 71 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 284 1056 1650
Size: 284 772 594
Fragments arranged by size:
772 594 284
BseYI C'CCAG_C
Cuts at: 0 158 605 701 1587 1650
Size: 158 447 96 886 63
Fragments arranged by size:
886 447 158 96 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1650
Size: 870 780
BsiEI CG_ry'CG
Cuts at: 0 1179 1650
Size: 1179 471
BsiHKAI G_wGCw'C
Cuts at: 0 53 1585 1650
Size: 53 1532 65
Fragments arranged by size:
1532 65 53
BsmI GAATG_Cn'
Cuts at: 0 616 1650
Size: 616 1034
Bsp1286I G_dGCh'C
Cuts at: 0 8 53 346 702 1559 1585 1650
Size: 8 45 293 356 857 26 65
Fragments arranged by size:
857 356 293 65 45 26 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 439 898 1650
Size: 439 459 752
Fragments arranged by size:
752 459 439
BsrBI CCG'CTC
Cuts at: 0 401 519 590 1577 1650
Size: 401 118 71 987 73
Fragments arranged by size:
987 401 118 73 71
BsrFI r'CCGG_y
Cuts at: 0 1106 1650
Size: 1106 544
BssSI C'ACGA_G
Cuts at: 0 46 1552 1650
Size: 46 1506 98
Fragments arranged by size:
1506 98 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1488 1650
Size: 1488 162
BstYI r'GATC_y
Cuts at: 0 246 1184 1650
Size: 246 938 466
Fragments arranged by size:
938 466 246
Bsu36I CC'TnA_GG
Cuts at: 0 860 1650
Size: 860 790
BtgI C'CryG_G
Cuts at: 0 250 894 1028 1650
Size: 250 644 134 622
Fragments arranged by size:
644 622 250 134
BtsI GCAGTG_nn'
Cuts at: 0 154 1084 1493 1600 1650
Size: 154 930 409 107 50
Fragments arranged by size:
930 409 154 107 50
DraIII CAC_nnn'GTG
Cuts at: 0 297 1650
Size: 297 1353
EaeI y'GGCC_r
Cuts at: 0 73 374 579 668 1031 1176 1323 1614
Size: 73 301 205 89 363 145 147 291
Cuts at: 1614 1650
Size: 36
Fragments arranged by size:
363 301 291 205 147 145 89 73
36
EagI C'GGCC_G
Cuts at: 0 1176 1650
Size: 1176 474
EarI CTCTTCn'nnn_
Cuts at: 0 43 633 1099 1650
Size: 43 590 466 551
Fragments arranged by size:
590 551 466 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1331 1650
Size: 1331 319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 614 691 1498 1650
Size: 306 308 77 807 152
Fragments arranged by size:
807 308 306 152 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 1650
Size: 496 1154
EcoO109I rG'GnC_Cy
Cuts at: 0 938 966 1502 1650
Size: 938 28 536 148
Fragments arranged by size:
938 536 148 28
HaeII r_GCGC'y
Cuts at: 0 26 441 1125 1476 1547 1650
Size: 26 415 684 351 71 103
Fragments arranged by size:
684 415 351 103 71 26
HincII GTy'rAC
Cuts at: 0 816 1650
Size: 816 834
KasI G'GCGC_C
Cuts at: 0 22 1121 1472 1650
Size: 22 1099 351 178
Fragments arranged by size:
1099 351 178 22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1650
Size: 511 1139
MscI TGG'CCA
Cuts at: 0 1033 1650
Size: 1033 617
MslI CAynn'nnrTG
Cuts at: 0 789 1650
Size: 789 861
MspA1I CmG'CkG
Cuts at: 0 20 69 116 252 380 752 1037 1058
Size: 20 49 47 136 128 372 285 21
Cuts at: 1058 1119 1236 1591 1650
Size: 61 117 355 59
Fragments arranged by size:
372 355 285 136 128 117 61 59
49 47 21 20
NarI GG'CG_CC
Cuts at: 0 23 1122 1473 1650
Size: 23 1099 351 177
Fragments arranged by size:
1099 351 177 23
NcoI C'CATG_G
Cuts at: 0 894 1028 1650
Size: 894 134 622
Fragments arranged by size:
894 622 134
NspI r_CATG'y
Cuts at: 0 812 1650
Size: 812 838
PasI CC'CwG_GG
Cuts at: 0 94 942 1438 1650
Size: 94 848 496 212
Fragments arranged by size:
848 496 212 94
PciI A'CATG_T
Cuts at: 0 808 1650
Size: 808 842
PflMI CCAn_nnn'nTGG
Cuts at: 0 1438 1612 1650
Size: 1438 174 38
Fragments arranged by size:
1438 174 38
PpuMI rG'GwC_Cy
Cuts at: 0 966 1650
Size: 966 684
PstI C_TGCA'G
Cuts at: 0 1395 1596 1650
Size: 1395 201 54
Fragments arranged by size:
1395 201 54
PvuII CAG'CTG
Cuts at: 0 380 752 1058 1591 1650
Size: 380 372 306 533 59
Fragments arranged by size:
533 380 372 306 59
SacII CC_GC'GG
Cuts at: 0 253 1650
Size: 253 1397
SapI GCTCTTCn'nnn_
Cuts at: 0 43 633 1099 1650
Size: 43 590 466 551
Fragments arranged by size:
590 551 466 43
SfcI C'TryA_G
Cuts at: 0 1391 1592 1650
Size: 1391 201 58
Fragments arranged by size:
1391 201 58
SfoI GGC'GCC
Cuts at: 0 24 1123 1474 1650
Size: 24 1099 351 176
Fragments arranged by size:
1099 351 176 24
StuI AGG'CCT
Cuts at: 0 1357 1650
Size: 1357 293
StyI C'CwwG_G
Cuts at: 0 8 333 593 894 1028 1650
Size: 8 325 260 301 134 622
Fragments arranged by size:
622 325 301 260 134 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1443 1475 1650
Size: 1443 32 175
Fragments arranged by size:
1443 175 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 442 1650
Size: 442 1208
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AloI AlwNI AvaI
AvrII BamHI BanI BbeI BbsI BcgI BglI Bme1580I
BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI
BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII HincII KasI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PciI PflMI PpuMI PstI PvuII SacII SapI SfcI
SfoI StuI StyI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BanII
BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI