Restriction map for
pF1KSDA0170
(Linear) (Six-base) MAPSORT of: KIAA0170.seq Check: 2987 from: 1 to: 6267
KIAA0170.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3045 6267
Size: 3045 3222
AccI GT'mk_AC
Cuts at: 0 2445 6267
Size: 2445 3822
Acc65I G'GTAC_C
Cuts at: 0 2221 6267
Size: 2221 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 770 2072 2093 3149 4717 6229 6267
Size: 764 6 1302 21 1056 1568 1512 38
Fragments arranged by size:
1568 1512 1302 1056 764 38 21 6
AfeI AGC'GCT
Cuts at: 0 1238 6267
Size: 1238 5029
AflII C'TTAA_G
Cuts at: 0 1843 6267
Size: 1843 4424
AflIII A'CryG_T
Cuts at: 0 1311 2028 6267
Size: 1311 717 4239
Fragments arranged by size:
4239 1311 717
AleI CACnn'nnGTG
Cuts at: 0 661 904 1366 1552 1570 5695 6267
Size: 661 243 462 186 18 4125 572
Fragments arranged by size:
4125 661 572 462 243 186 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3467 3499 3713 3745 5176 5208 6267
Size: 3467 32 214 32 1431 32 1059
Fragments arranged by size:
3467 1431 1059 214 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2121 2905 3127 3496 3742 3865 3988 4357
Size: 2121 784 222 369 246 123 123 369
Cuts at: 4357 4480 4603 4849 4972 6267
Size: 123 123 246 123 1295
Fragments arranged by size:
2121 1295 784 369 369 246 246 222
123 123 123 123 123
ApaI G_GGCC'C
Cuts at: 0 430 1875 2476 6267
Size: 430 1445 601 3791
Fragments arranged by size:
3791 1445 601 430
ApoI r'AATT_y
Cuts at: 0 5095 6267
Size: 5095 1172
AvaI C'yCGr_G
Cuts at: 0 151 422 4166 4240 4658 4781 5708 5969
Size: 151 271 3744 74 418 123 927 261
Cuts at: 5969 6267
Size: 298
Fragments arranged by size:
3744 927 418 298 271 261 151 123
74
AvrII C'CTAG_G
Cuts at: 0 2344 6267
Size: 2344 3923
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 803 836 1121 1154 6267
Size: 803 33 285 33 5113
Fragments arranged by size:
5113 803 285 33 33
BamHI G'GATC_C
Cuts at: 0 1010 1520 2980 6267
Size: 1010 510 1460 3287
Fragments arranged by size:
3287 1460 1010 510
BanI G'GyrC_C
Cuts at: 0 115 255 718 1750 2221 2808 6267
Size: 115 140 463 1032 471 587 3459
Fragments arranged by size:
3459 1032 587 471 463 140 115
BanII G_rGCy'C
Cuts at: 0 325 430 963 1385 1437 1513 1860 1875
Size: 325 105 533 422 52 76 347 15
Cuts at: 1875 1998 2380 2476 2995 3380 3503 3545 3626
Size: 123 382 96 519 385 123 42 81
Cuts at: 3626 3749 3791 3872 3995 4118 4160 4283 4364
Size: 123 42 81 123 123 42 123 81
Cuts at: 4364 4529 4610 4652 4775 4856 5408 5618 5970
Size: 165 81 42 123 81 552 210 352
Cuts at: 5970 6267
Size: 297
Fragments arranged by size:
552 533 519 422 385 382 352 347
325 297 210 165 123 123 123 123
123 123 123 105 96 81 81 81
81 81 76 52 42 42 42 42
15
BbsI GAAGACnn'nnnn_
Cuts at: 0 2060 4333 5048 5509 6267
Size: 2060 2273 715 461 758
Fragments arranged by size:
2273 2060 758 715 461
BbvCI CC'TCA_GC
Cuts at: 0 494 702 941 2940 6267
Size: 494 208 239 1999 3327
Fragments arranged by size:
3327 1999 494 239 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1010 6267
Size: 976 34 5257
Fragments arranged by size:
5257 976 34
BclI T'GATC_A
Cuts at: 0 6116 6267
Size: 6116 151
BfrBI ATG'CAT
Cuts at: 0 1608 6267
Size: 1608 4659
BglI GCCn_nnn'nGGC
Cuts at: 0 93 1159 6267
Size: 93 1066 5108
Fragments arranged by size:
5108 1066 93
BglII A'GATC_T
Cuts at: 0 1052 1938 2076 5386 5999 6267
Size: 1052 886 138 3310 613 268
Fragments arranged by size:
3310 1052 886 613 268 138
BlpI GC'TnA_GC
Cuts at: 0 962 6267
Size: 962 5305
Bme1580I G_kGCm'C
Cuts at: 0 120 430 1753 1875 2476 2813 3611 3857
Size: 120 310 1323 122 601 337 798 246
Cuts at: 3857 4595 5820 6267
Size: 738 1225 447
Fragments arranged by size:
1323 1225 798 738 601 447 337 310
246 122 120
BmgBI CAC'GTC
Cuts at: 0 1368 6267
Size: 1368 4899
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 1018 1381 2091 2374 2980 5147 5743
Size: 33 985 363 710 283 606 2167 596
Cuts at: 5743 6267
Size: 524
Fragments arranged by size:
2167 985 710 606 596 524 363 283
33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 714 717 896 1770 1948 5337 5984 6267
Size: 714 3 179 874 178 3389 647 283
Fragments arranged by size:
3389 874 714 647 283 179 178 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 905 1103 1192 1549 2128 2845 2871
Size: 886 19 198 89 357 579 717 26
Cuts at: 2871 3083 3251 3313 3321 3487 3610 3733 3979
Size: 212 168 62 8 166 123 123 246
Cuts at: 3979 4348 4594 4731 5392 6035 6221 6267
Size: 369 246 137 661 643 186 46
Fragments arranged by size:
886 717 661 643 579 369 357 246
246 212 198 186 168 166 137 123
123 89 62 46 26 19 8
Bpu10I CC'TnA_GC
Cuts at: 0 494 702 941 2940 3374 3497 3743 3866
Size: 494 208 239 1999 434 123 246 123
Cuts at: 3866 3989 4031 4112 4154 4235 4277 4358 4400
Size: 123 42 81 42 81 42 81 42
Cuts at: 4400 4481 4604 4646 4727 4769 4826 4850 5027
Size: 81 123 42 81 42 57 24 177
Cuts at: 5027 5135 5312 5918 6267
Size: 108 177 606 349
Fragments arranged by size:
1999 606 494 434 349 246 239 208
177 177 123 123 123 123 108 81
81 81 81 81 57 42 42 42
42 42 42 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 1396 1905 2335 3640 4999 5241 5356
Size: 90 1306 509 430 1305 1359 242 115
Cuts at: 5356 6267
Size: 911
Fragments arranged by size:
1359 1306 1305 911 509 430 242 115
90
BsaI GGTCTCn'nnnn_
Cuts at: 0 297 2284 2354 2754 5591 6267
Size: 297 1987 70 400 2837 676
Fragments arranged by size:
2837 1987 676 400 297 70
BsaBI GATnn'nnATC
Cuts at: 0 235 6267
Size: 235 6032
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 248 278 1985 2015 5176 5206 6267
Size: 248 30 1707 30 3161 30 1061
Fragments arranged by size:
3161 1707 1061 248 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 58 520 690 706 885 1387 1759
Size: 55 3 462 170 16 179 502 372
Cuts at: 1759 5623 5626 6166 6267
Size: 3864 3 540 101
Fragments arranged by size:
3864 540 502 462 372 179 170 101
55 16 3 3
BseYI C'CCAG_C
Cuts at: 0 853 1014 1816 1976 2294 3135 3401 5179
Size: 853 161 802 160 318 841 266 1778
Cuts at: 5179 5327 6079 6267
Size: 148 752 188
Fragments arranged by size:
1778 853 841 802 752 318 266 188
161 160 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 4815 5213 6267
Size: 851 3964 398 1054
Fragments arranged by size:
3964 1054 851 398
BsiEI CG_ry'CG
Cuts at: 0 3648 6267
Size: 3648 2619
BsiHKAI G_wGCw'C
Cuts at: 0 1089 1385 1449 1513 1860 3503 3626 3749
Size: 1089 296 64 64 347 1643 123 123
Cuts at: 3749 3872 3995 4118 4364 4610 5408 5687 6267
Size: 123 123 123 246 246 798 279 580
Fragments arranged by size:
1643 1089 798 580 347 296 279 246
246 123 123 123 123 123 64 64
BsmI GAATG_Cn'
Cuts at: 0 179 600 6267
Size: 179 421 5667
Fragments arranged by size:
5667 421 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4415 6267
Size: 4415 1852
Bsp1286I G_dGCh'C
Cuts at: 0 120 325 430 963 1089 1385 1437 1449
Size: 120 205 105 533 126 296 52 12
Cuts at: 1449 1513 1753 1860 1875 1998 2380 2476 2813
Size: 64 240 107 15 123 382 96 337
Cuts at: 2813 2995 3380 3503 3545 3611 3626 3749 3791
Size: 182 385 123 42 66 15 123 42
Cuts at: 3791 3857 3872 3995 4118 4160 4283 4364 4529
Size: 66 15 123 123 42 123 81 165
Cuts at: 4529 4595 4610 4652 4775 4856 5408 5618 5687
Size: 66 15 42 123 81 552 210 69
Cuts at: 5687 5820 5970 6267
Size: 133 150 297
Fragments arranged by size:
552 533 385 382 337 297 296 240
210 205 182 165 150 133 126 123
123 123 123 123 123 123 120 107
105 96 81 81 69 66 66 66
64 52 42 42 42 42 15 15
15 15 12
BspHI T'CATG_A
Cuts at: 0 1860 6267
Size: 1860 4407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3045 5371 6267
Size: 3045 2326 896
Fragments arranged by size:
3045 2326 896
BsrBI CCG'CTC
Cuts at: 0 5718 6267
Size: 5718 549
BsrDI GCAATG_nn'
Cuts at: 0 2199 6141 6267
Size: 2199 3942 126
Fragments arranged by size:
3942 2199 126
BsrFI r'CCGG_y
Cuts at: 0 5076 6267
Size: 5076 1191
BstEII G'GTnAC_C
Cuts at: 0 5907 6267
Size: 5907 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 883 1554 2188 2971 6267
Size: 883 671 634 783 3296
Fragments arranged by size:
3296 883 783 671 634
BstYI r'GATC_y
Cuts at: 0 1010 1052 1520 1938 2076 2980 3577 3700
Size: 1010 42 468 418 138 904 597 123
Cuts at: 3700 3823 4192 4438 5360 5386 5999 6267
Size: 123 369 246 922 26 613 268
Fragments arranged by size:
1010 922 904 613 597 468 418 369
268 246 138 123 123 42 26
BstZ17I GTA'TAC
Cuts at: 0 2446 6267
Size: 2446 3821
Bsu36I CC'TnA_GG
Cuts at: 0 311 5015 5414 6128 6267
Size: 311 4704 399 714 139
Fragments arranged by size:
4704 714 399 311 139
BtgI C'CryG_G
Cuts at: 0 120 1059 1190 2236 5450 6267
Size: 120 939 131 1046 3214 817
Fragments arranged by size:
3214 1046 939 817 131 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 376 6267
Size: 376 5891
BtsI GCAGTG_nn'
Cuts at: 0 5285 6267
Size: 5285 982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3600 3635 6267
Size: 3600 35 2632
Fragments arranged by size:
3600 2632 35
DraIII CAC_nnn'GTG
Cuts at: 0 4793 6267
Size: 4793 1474
DrdI GACnn_nn'nnGTC
Cuts at: 0 3453 3529 3652 3775 3898 3945 4021 4068
Size: 3453 76 123 123 123 47 76 47
Cuts at: 4068 4267 4314 4513 4636 4683 4806 4882 4929
Size: 199 47 199 123 47 123 76 47
Cuts at: 4929 5005 6267
Size: 76 1262
Fragments arranged by size:
3453 1262 199 199 123 123 123 123
123 76 76 76 76 47 47 47
47 47
EarI CTCTTCn'nnn_
Cuts at: 0 32 437 587 708 998 1039 1289 1373
Size: 32 405 150 121 290 41 250 84
Cuts at: 1373 2795 5516 5552 5692 6267
Size: 1422 2721 36 140 575
Fragments arranged by size:
2721 1422 575 405 290 250 150 140
121 84 41 36 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3179 4485 5782 6267
Size: 3179 1306 1297 485
Fragments arranged by size:
3179 1306 1297 485
EcoICRI GAG'CTC
Cuts at: 0 1383 1511 1858 3501 3624 3747 3870 3993
Size: 1383 128 347 1643 123 123 123 123
Cuts at: 3993 4116 4362 4608 5406 6267
Size: 123 246 246 798 861
Fragments arranged by size:
1643 1383 861 798 347 246 246 128
123 123 123 123 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 770 886 905 1103 1192 1549 2072
Size: 764 6 116 19 198 89 357 523
Cuts at: 2072 2093 2128 2845 2871 3083 3149 3251 3313
Size: 21 35 717 26 212 66 102 62
Cuts at: 3313 3321 3487 3610 3733 3979 4348 4594 4717
Size: 8 166 123 123 246 369 246 123
Cuts at: 4717 4731 5392 6035 6221 6229 6267
Size: 14 661 643 186 8 38
Fragments arranged by size:
764 717 661 643 523 369 357 246
246 212 198 186 166 123 123 123
116 102 89 66 62 38 35 26
21 19 14 8 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2480 6267
Size: 2480 3787
EcoO109I rG'GnC_Cy
Cuts at: 0 426 427 1078 1152 1871 1908 2001 2118
Size: 426 1 651 74 719 37 93 117
Cuts at: 2118 2244 2473 2922 2932 3455 4316 4685 4808
Size: 126 229 449 10 523 861 369 123
Cuts at: 4808 4931 6267
Size: 123 1336
Fragments arranged by size:
1336 861 719 651 523 449 426 369
229 126 123 123 117 93 74 37
10 1
EcoRI G'AATT_C
Cuts at: 0 5095 6267
Size: 5095 1172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1202 6267
Size: 1202 5065
HaeII r_GCGC'y
Cuts at: 0 1240 6267
Size: 1240 5027
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 324 356 1391 1423 1495 1527 1985 2017
Size: 324 32 1035 32 72 32 458 32
Cuts at: 2017 3079 3111 3532 3564 3569 3601 3692 3724
Size: 1062 32 421 32 5 32 91 32
Cuts at: 3724 3815 3847 4147 4179 4270 4302 4393 4425
Size: 91 32 300 32 91 32 91 32
Cuts at: 4425 4426 4430 4458 4462 4516 4548 4639 4671
Size: 1 4 28 4 54 32 91 32
Cuts at: 4671 4762 4794 5477 5509 6101 6133 6244 6267
Size: 91 32 683 32 592 32 111 23
Fragments arranged by size:
1062 1035 683 592 458 421 324 300
111 91 91 91 91 91 91 72
54 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 28 23 5 4 4 1
HincII GTy'rAC
Cuts at: 0 2171 6267
Size: 2171 4096
HindIII A'AGCT_T
Cuts at: 0 1184 6267
Size: 1184 5083
KpnI G_GTAC'C
Cuts at: 0 2225 6267
Size: 2225 4042
MfeI C'AATT_G
Cuts at: 0 4709 6267
Size: 4709 1558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5667 6267
Size: 5667 600
MslI CAynn'nnrTG
Cuts at: 0 661 904 1036 1366 1552 1570 2033 5695
Size: 661 243 132 330 186 18 463 3662
Cuts at: 5695 6267
Size: 572
Fragments arranged by size:
3662 661 572 463 330 243 186 132
18
MspA1I CmG'CkG
Cuts at: 0 706 853 940 1837 5179 5290 5758 6267
Size: 706 147 87 897 3342 111 468 509
Fragments arranged by size:
3342 897 706 509 468 147 111 87
NcoI C'CATG_G
Cuts at: 0 120 1059 1190 2236 5450 6267
Size: 120 939 131 1046 3214 817
Fragments arranged by size:
3214 1046 939 817 131 120
NsiI A_TGCA'T
Cuts at: 0 1610 6267
Size: 1610 4657
NspI r_CATG'y
Cuts at: 0 226 1305 2032 6087 6125 6267
Size: 226 1079 727 4055 38 142
Fragments arranged by size:
4055 1079 727 226 142 38
PasI CC'CwG_GG
Cuts at: 0 325 1875 5820 6267
Size: 325 1550 3945 447
Fragments arranged by size:
3945 1550 447 325
PciI A'CATG_T
Cuts at: 0 2028 6267
Size: 2028 4239
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 1314 2139 2905 5370 6267
Size: 396 918 825 766 2465 897
Fragments arranged by size:
2465 918 897 825 766 396
PfoI T'CCnGG_A
Cuts at: 0 882 927 1080 2822 2928 3052 6267
Size: 882 45 153 1742 106 124 3215
Fragments arranged by size:
3215 1742 882 153 124 106 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5176 5208 6267
Size: 5176 32 1059
Fragments arranged by size:
5176 1059 32
PpuMI rG'GwC_Cy
Cuts at: 0 1078 1908 2001 2244 2922 2932 3455 4316
Size: 1078 830 93 243 678 10 523 861
Cuts at: 4316 4685 4808 4931 6267
Size: 369 123 123 1336
Fragments arranged by size:
1336 1078 861 830 678 523 369 243
123 123 93 10
PspOMI G'GGCC_C
Cuts at: 0 426 1871 2472 6267
Size: 426 1445 601 3795
Fragments arranged by size:
3795 1445 601 426
PspXI vC'TCGA_Gb
Cuts at: 0 4240 6267
Size: 4240 2027
PstI C_TGCA'G
Cuts at: 0 1109 1328 1836 3123 5541 6267
Size: 1109 219 508 1287 2418 726
Fragments arranged by size:
2418 1287 1109 726 508 219
PvuII CAG'CTG
Cuts at: 0 706 853 940 1837 5290 6267
Size: 706 147 87 897 3453 977
Fragments arranged by size:
3453 977 897 706 147 87
SacI G_AGCT'C
Cuts at: 0 1385 1513 1860 3503 3626 3749 3872 3995
Size: 1385 128 347 1643 123 123 123 123
Cuts at: 3995 4118 4364 4610 5408 6267
Size: 123 246 246 798 859
Fragments arranged by size:
1643 1385 859 798 347 246 246 128
123 123 123 123 123
SapI GCTCTTCn'nnn_
Cuts at: 0 1373 5692 6267
Size: 1373 4319 575
Fragments arranged by size:
4319 1373 575
SexAI A'CCwGG_T
Cuts at: 0 5774 6267
Size: 5774 493
SfcI C'TryA_G
Cuts at: 0 725 1105 1324 1832 3119 4103 4486 4718
Size: 725 380 219 508 1287 984 383 232
Cuts at: 4718 5537 6267
Size: 819 730
Fragments arranged by size:
1287 984 819 730 725 508 383 380
232 219
SmaI CCC'GGG
Cuts at: 0 424 6267
Size: 424 5843
SmlI C'TyrA_G
Cuts at: 0 69 1411 1843 1920 2314 3619 4240 4978
Size: 69 1342 432 77 394 1305 621 738
Cuts at: 4978 5256 5335 6267
Size: 278 79 932
Fragments arranged by size:
1342 1305 932 738 621 432 394 278
79 77 69
SphI G_CATG'C
Cuts at: 0 6087 6267
Size: 6087 180
StuI AGG'CCT
Cuts at: 0 697 924 1094 2161 2320 2884 3014 5254
Size: 697 227 170 1067 159 564 130 2240
Cuts at: 5254 5419 6232 6267
Size: 165 813 35
Fragments arranged by size:
2240 1067 813 697 564 227 170 165
159 130 35
StyI C'CwwG_G
Cuts at: 0 120 621 1059 1190 2042 2236 2344 2558
Size: 120 501 438 131 852 194 108 214
Cuts at: 2558 5234 5450 6267
Size: 2676 216 817
Fragments arranged by size:
2676 852 817 501 438 216 214 194
131 120 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 465 1990 2023 2620 3320 3662 3908
Size: 25 440 1525 33 597 700 342 246
Cuts at: 3908 4550 4646 5926 6080 6267
Size: 642 96 1280 154 187
Fragments arranged by size:
1525 1280 700 642 597 440 342 246
187 154 96 33 25
TatI w'GTAC_w
Cuts at: 0 456 2712 6267
Size: 456 2256 3555
Fragments arranged by size:
3555 2256 456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 248 280 1148 1180 1505 1537 2972 3004
Size: 248 32 868 32 325 32 1435 32
Cuts at: 3004 6267
Size: 3263
Fragments arranged by size:
3263 1435 868 325 248 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1698 6267
Size: 1698 4569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1471 2820 5844 6267
Size: 1471 1349 3024 423
Fragments arranged by size:
3024 1471 1349 423
XhoI C'TCGA_G
Cuts at: 0 4240 6267
Size: 4240 2027
XmaI C'CCGG_G
Cuts at: 0 422 6267
Size: 422 5845
XmnI GAAnn'nnTTC
Cuts at: 0 359 1054 6267
Size: 359 695 5213
Fragments arranged by size:
5213 695 359
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AleI
AloI AlwNI ApaI ApoI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KpnI MfeI MmeI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SapI SexAI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AgeI AhdI AlfI ApaLI AscI AseI
AsiSI BbeI BciVI BmtI BsaAI BsaHI BsaWI BsiWI
BspEI BsrGI BssHII BssSI BstAPI BstBI ClaI DraI
EaeI EagI EcoRV FseI FspI FspAI HpaI KasI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
ZraI