Restriction map for
pF1KSDA0174
(Linear) (Six-base) MAPSORT of: KIAA0174.seq Check: 1520 from: 1 to: 1092
KIAA0174.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 301 1092
Size: 301 791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1092
Size: 1055 37
AfeI AGC'GCT
Cuts at: 0 28 1092
Size: 28 1064
AflII C'TTAA_G
Cuts at: 0 30 1092
Size: 30 1062
AlwNI CAG_nnn'CTG
Cuts at: 0 1033 1092
Size: 1033 59
ApoI r'AATT_y
Cuts at: 0 39 1092
Size: 39 1053
AseI AT'TA_AT
Cuts at: 0 857 1092
Size: 857 235
BamHI G'GATC_C
Cuts at: 0 158 1092
Size: 158 934
BanI G'GyrC_C
Cuts at: 0 681 1015 1092
Size: 681 334 77
Fragments arranged by size:
681 334 77
BanII G_rGCy'C
Cuts at: 0 10 156 1092
Size: 10 146 936
Fragments arranged by size:
936 146 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 1043 1092
Size: 191 852 49
Fragments arranged by size:
852 191 49
BbvCI CC'TCA_GC
Cuts at: 0 1026 1092
Size: 1026 66
BclI T'GATC_A
Cuts at: 0 363 1092
Size: 363 729
BglII A'GATC_T
Cuts at: 0 579 1092
Size: 579 513
BlpI GC'TnA_GC
Cuts at: 0 23 1092
Size: 23 1069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 313 1092
Size: 235 78 779
Fragments arranged by size:
779 235 78
Bpu10I CC'TnA_GC
Cuts at: 0 1026 1092
Size: 1026 66
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 311 551 647 1092
Size: 311 240 96 445
Fragments arranged by size:
445 311 240 96
BseYI C'CCAG_C
Cuts at: 0 3 136 1092
Size: 3 133 956
Fragments arranged by size:
956 133 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1092
Size: 866 226
BsiHKAI G_wGCw'C
Cuts at: 0 156 173 1092
Size: 156 17 919
Fragments arranged by size:
919 156 17
Bsp1286I G_dGCh'C
Cuts at: 0 10 156 173 1092
Size: 10 146 17 919
Fragments arranged by size:
919 146 17 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 948 1092
Size: 239 709 144
Fragments arranged by size:
709 239 144
BssSI C'ACGA_G
Cuts at: 0 193 1092
Size: 193 899
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 1092
Size: 480 612
BstYI r'GATC_y
Cuts at: 0 158 209 579 1092
Size: 158 51 370 513
Fragments arranged by size:
513 370 158 51
BtgI C'CryG_G
Cuts at: 0 203 1092
Size: 203 889
BtsI GCAGTG_nn'
Cuts at: 0 736 1092
Size: 736 356
DraI TTT'AAA
Cuts at: 0 18 1092
Size: 18 1074
DrdI GACnn_nn'nnGTC
Cuts at: 0 544 961 1092
Size: 544 417 131
Fragments arranged by size:
544 417 131
EarI CTCTTCn'nnn_
Cuts at: 0 624 882 1067 1092
Size: 624 258 185 25
Fragments arranged by size:
624 258 185 25
EcoICRI GAG'CTC
Cuts at: 0 154 1092
Size: 154 938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 313 1055 1092
Size: 235 78 742 37
Fragments arranged by size:
742 235 78 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 629 1092
Size: 196 433 463
Fragments arranged by size:
463 433 196
EcoO109I rG'GnC_Cy
Cuts at: 0 622 1092
Size: 622 470
HaeII r_GCGC'y
Cuts at: 0 30 1092
Size: 30 1062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 879 911 964 996 1092
Size: 143 32 704 32 53 32 96
Fragments arranged by size:
704 143 96 53 32 32 32
HindIII A'AGCT_T
Cuts at: 0 925 1092
Size: 925 167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1092
Size: 573 519
MslI CAynn'nnrTG
Cuts at: 0 697 703 1092
Size: 697 6 389
Fragments arranged by size:
697 389 6
NcoI C'CATG_G
Cuts at: 0 203 1092
Size: 203 889
PflMI CCAn_nnn'nTGG
Cuts at: 0 664 1092
Size: 664 428
PfoI T'CCnGG_A
Cuts at: 0 179 212 897 1060 1092
Size: 179 33 685 163 32
Fragments arranged by size:
685 179 163 33 32
PshAI GACnn'nnGTC
Cuts at: 0 330 1092
Size: 330 762
PstI C_TGCA'G
Cuts at: 0 945 1092
Size: 945 147
SacI G_AGCT'C
Cuts at: 0 156 1092
Size: 156 936
SapI GCTCTTCn'nnn_
Cuts at: 0 1067 1092
Size: 1067 25
SfcI C'TryA_G
Cuts at: 0 845 941 1092
Size: 845 96 151
Fragments arranged by size:
845 151 96
SmlI C'TyrA_G
Cuts at: 0 30 1092
Size: 30 1062
SspI AAT'ATT
Cuts at: 0 801 1092
Size: 801 291
StuI AGG'CCT
Cuts at: 0 790 1092
Size: 790 302
StyI C'CwwG_G
Cuts at: 0 203 1092
Size: 203 889
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1092
Size: 486 606
TatI w'GTAC_w
Cuts at: 0 221 1092
Size: 221 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 461 493 1092
Size: 461 32 599
Fragments arranged by size:
599 461 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 480 1092
Size: 210 270 612
Fragments arranged by size:
612 270 210
Enzymes that do cut:
AccI AcuI AfeI AflII AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BglII BlpI BpmI
Bpu10I BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BssSI
BstXI BstYI BtgI BtsI DraI DrdI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MslI
NcoI PflMI PfoI PshAI PstI SacI SapI SfcI
SmlI SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BfrBI BglI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI