Restriction map for
pF1KSDA0180
(Linear) (Six-base) MAPSORT of: KIAA0180.seq Check: 4247 from: 1 to: 1164
KIAA0180.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 917 1016 1164
Size: 917 99 148
Fragments arranged by size:
917 148 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 775 880 1015 1164
Size: 492 283 105 135 149
Fragments arranged by size:
492 283 149 135 105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 730 1164
Size: 730 434
AvaI C'yCGr_G
Cuts at: 0 63 1164
Size: 63 1101
BanI G'GyrC_C
Cuts at: 0 4 336 1164
Size: 4 332 828
Fragments arranged by size:
828 332 4
BanII G_rGCy'C
Cuts at: 0 82 160 284 585 956 1164
Size: 82 78 124 301 371 208
Fragments arranged by size:
371 301 208 124 82 78
BbeI G_GCGC'C
Cuts at: 0 8 1164
Size: 8 1156
BbsI GAAGACnn'nnnn_
Cuts at: 0 418 664 1109 1164
Size: 418 246 445 55
Fragments arranged by size:
445 418 246 55
BglI GCCn_nnn'nGGC
Cuts at: 0 16 1164
Size: 16 1148
BlpI GC'TnA_GC
Cuts at: 0 28 1164
Size: 28 1136
Bme1580I G_kGCm'C
Cuts at: 0 21 421 1164
Size: 21 400 743
Fragments arranged by size:
743 400 21
BmgBI CAC'GTC
Cuts at: 0 416 1164
Size: 416 748
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 903 1164
Size: 585 318 261
Fragments arranged by size:
585 318 261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 563 1164
Size: 563 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 298 1087 1164
Size: 271 27 789 77
Fragments arranged by size:
789 271 77 27
Bpu10I CC'TnA_GC
Cuts at: 0 950 1145 1164
Size: 950 195 19
Fragments arranged by size:
950 195 19
BsaHI Gr'CG_yC
Cuts at: 0 5 1164
Size: 5 1159
BsaWI w'CCGG_w
Cuts at: 0 561 1164
Size: 561 603
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1048 1078 1164
Size: 1048 30 86
Fragments arranged by size:
1048 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1056 1164
Size: 1056 108
BseYI C'CCAG_C
Cuts at: 0 392 585 1164
Size: 392 193 579
Fragments arranged by size:
579 392 193
BsiEI CG_ry'CG
Cuts at: 0 25 95 1164
Size: 25 70 1069
Fragments arranged by size:
1069 70 25
BsiHKAI G_wGCw'C
Cuts at: 0 956 1164
Size: 956 208
BsmI GAATG_Cn'
Cuts at: 0 707 1164
Size: 707 457
Bsp1286I G_dGCh'C
Cuts at: 0 21 82 160 284 421 585 956 1164
Size: 21 61 78 124 137 164 371 208
Fragments arranged by size:
371 208 164 137 124 78 61 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 1164
Size: 110 1054
BsrBI CCG'CTC
Cuts at: 0 60 1095 1164
Size: 60 1035 69
Fragments arranged by size:
1035 69 60
BsrFI r'CCGG_y
Cuts at: 0 94 1164
Size: 94 1070
BsrGI T'GTAC_A
Cuts at: 0 1022 1164
Size: 1022 142
BstEII G'GTnAC_C
Cuts at: 0 97 1164
Size: 97 1067
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1051 1164
Size: 1051 113
BstYI r'GATC_y
Cuts at: 0 866 1164
Size: 866 298
Bsu36I CC'TnA_GG
Cuts at: 0 1001 1164
Size: 1001 163
BtsI GCAGTG_nn'
Cuts at: 0 802 1073 1164
Size: 802 271 91
Fragments arranged by size:
802 271 91
EaeI y'GGCC_r
Cuts at: 0 22 92 1032 1164
Size: 22 70 940 132
Fragments arranged by size:
940 132 70 22
EagI C'GGCC_G
Cuts at: 0 22 92 1164
Size: 22 70 1072
Fragments arranged by size:
1072 70 22
EarI CTCTTCn'nnn_
Cuts at: 0 970 1164
Size: 970 194
EcoICRI GAG'CTC
Cuts at: 0 954 1164
Size: 954 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 298 492 775 880 1015 1087 1164
Size: 271 27 194 283 105 135 72 77
Fragments arranged by size:
283 271 194 135 105 77 72 27
EcoO109I rG'GnC_Cy
Cuts at: 0 87 1098 1139 1164
Size: 87 1011 41 25
Fragments arranged by size:
1011 87 41 25
FspI TGC'GCA
Cuts at: 0 718 1164
Size: 718 446
HaeII r_GCGC'y
Cuts at: 0 8 129 1164
Size: 8 121 1035
Fragments arranged by size:
1035 121 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 1164
Size: 895 32 237
Fragments arranged by size:
895 237 32
HincII GTy'rAC
Cuts at: 0 1017 1164
Size: 1017 147
KasI G'GCGC_C
Cuts at: 0 4 1164
Size: 4 1160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 981 1164
Size: 464 517 183
Fragments arranged by size:
517 464 183
MspA1I CmG'CkG
Cuts at: 0 27 823 1164
Size: 27 796 341
Fragments arranged by size:
796 341 27
NarI GG'CG_CC
Cuts at: 0 5 1164
Size: 5 1159
PflMI CCAn_nnn'nTGG
Cuts at: 0 347 1164
Size: 347 817
PpuMI rG'GwC_Cy
Cuts at: 0 87 1098 1164
Size: 87 1011 66
Fragments arranged by size:
1011 87 66
PstI C_TGCA'G
Cuts at: 0 771 1164
Size: 771 393
SacI G_AGCT'C
Cuts at: 0 956 1164
Size: 956 208
SalI G'TCGA_C
Cuts at: 0 1015 1164
Size: 1015 149
SanDI GG'GwC_CC
Cuts at: 0 87 1164
Size: 87 1077
SapI GCTCTTCn'nnn_
Cuts at: 0 970 1164
Size: 970 194
ScaI AGT'ACT
Cuts at: 0 268 1164
Size: 268 896
SfcI C'TryA_G
Cuts at: 0 519 622 767 807 1164
Size: 519 103 145 40 357
Fragments arranged by size:
519 357 145 103 40
SfoI GGC'GCC
Cuts at: 0 6 1164
Size: 6 1158
SmaI CCC'GGG
Cuts at: 0 65 1164
Size: 65 1099
StyI C'CwwG_G
Cuts at: 0 191 205 1164
Size: 191 14 959
Fragments arranged by size:
959 191 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 358 879 903 1164
Size: 354 4 521 24 261
Fragments arranged by size:
521 354 261 24 4
TatI w'GTAC_w
Cuts at: 0 266 629 1022 1164
Size: 266 363 393 142
Fragments arranged by size:
393 363 266 142
XmaI C'CCGG_G
Cuts at: 0 63 1164
Size: 63 1101
XmnI GAAnn'nnTTC
Cuts at: 0 268 1164
Size: 268 896
Enzymes that do cut:
AccI AcuI AlfI AvaI BanI BanII BbeI BbsI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstEII BstXI BstYI
Bsu36I BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I HincII KasI MmeI MspA1I NarI
PflMI PpuMI PstI SacI SalI SanDI SapI ScaI
SfcI SfoI SmaI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BmtI BpuEI BsaI BsaAI BsaBI
BsgI BsiWI BsmBI BspEI BspHI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MfeI MluI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI