Restriction map for
pF1KA0189
(Linear) (Six-base) MAPSORT of: KIAA0189.seq Check: 1467 from: 1 to: 3069
KIAA0189.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1154 3069
Size: 899 255 1915
Fragments arranged by size:
1915 899 255
AfeI AGC'GCT
Cuts at: 0 2500 3069
Size: 2500 569
AflII C'TTAA_G
Cuts at: 0 2103 3069
Size: 2103 966
AflIII A'CryG_T
Cuts at: 0 941 3069
Size: 941 2128
AleI CACnn'nnGTG
Cuts at: 0 641 2602 2996 3069
Size: 641 1961 394 73
Fragments arranged by size:
1961 641 394 73
AlwNI CAG_nnn'CTG
Cuts at: 0 1469 1752 1800 1854 2091 2295 2315 2478
Size: 1469 283 48 54 237 204 20 163
Cuts at: 2478 2688 2829 3069
Size: 210 141 240
Fragments arranged by size:
1469 283 240 237 210 204 163 141
54 48 20
ApaI G_GGCC'C
Cuts at: 0 1128 2531 2655 2682 3055 3069
Size: 1128 1403 124 27 373 14
Fragments arranged by size:
1403 1128 373 124 27 14
ApaLI G'TGCA_C
Cuts at: 0 655 3069
Size: 655 2414
AvaI C'yCGr_G
Cuts at: 0 259 300 977 3069
Size: 259 41 677 2092
Fragments arranged by size:
2092 677 259 41
AvrII C'CTAG_G
Cuts at: 0 2257 3069
Size: 2257 812
BamHI G'GATC_C
Cuts at: 0 1844 2832 3069
Size: 1844 988 237
Fragments arranged by size:
1844 988 237
BanI G'GyrC_C
Cuts at: 0 571 779 1713 2166 2334 2559 2570 2742
Size: 571 208 934 453 168 225 11 172
Cuts at: 2742 3069
Size: 327
Fragments arranged by size:
934 571 453 327 225 208 172 168
11
BanII G_rGCy'C
Cuts at: 0 172 446 790 1128 1585 1961 2387 2531
Size: 172 274 344 338 457 376 426 144
Cuts at: 2531 2655 2682 2871 3055 3069
Size: 124 27 189 184 14
Fragments arranged by size:
457 426 376 344 338 274 189 184
172 144 124 27 14
BbeI G_GCGC'C
Cuts at: 0 783 2170 3069
Size: 783 1387 899
Fragments arranged by size:
1387 899 783
BbsI GAAGACnn'nnnn_
Cuts at: 0 68 3069
Size: 68 3001
BbvCI CC'TCA_GC
Cuts at: 0 319 555 872 1073 1175 1205 1382 3069
Size: 319 236 317 201 102 30 177 1687
Fragments arranged by size:
1687 319 317 236 201 177 102 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 236 3069
Size: 202 34 2833
Fragments arranged by size:
2833 202 34
BclI T'GATC_A
Cuts at: 0 2306 3069
Size: 2306 763
BglI GCCn_nnn'nGGC
Cuts at: 0 595 759 2243 2396 3069
Size: 595 164 1484 153 673
Fragments arranged by size:
1484 673 595 164 153
BglII A'GATC_T
Cuts at: 0 1997 3069
Size: 1997 1072
BlpI GC'TnA_GC
Cuts at: 0 851 2381 2386 2411 2495 3069
Size: 851 1530 5 25 84 574
Fragments arranged by size:
1530 851 574 84 25 5
Bme1580I G_kGCm'C
Cuts at: 0 294 659 1128 1628 2339 2531 2573 2655
Size: 294 365 469 500 711 192 42 82
Cuts at: 2655 2682 3055 3069
Size: 27 373 14
Fragments arranged by size:
711 500 469 373 365 294 192 82
42 27 14
BmgBI CAC'GTC
Cuts at: 0 1917 3069
Size: 1917 1152
BmrI ACTGGGnnnn_n'
Cuts at: 0 111 552 2817 2880 3069
Size: 111 441 2265 63 189
Fragments arranged by size:
2265 441 189 111 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 3069
Size: 492 2577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 919 1025 1154 1326 1393 1555 1978
Size: 52 867 106 129 172 67 162 423
Cuts at: 1978 3069
Size: 1091
Fragments arranged by size:
1091 867 423 172 162 129 106 67
52
Bpu10I CC'TnA_GC
Cuts at: 0 319 555 836 872 1073 1163 1175 1205
Size: 319 236 281 36 201 90 12 30
Cuts at: 1205 1382 1955 2446 3069
Size: 177 573 491 623
Fragments arranged by size:
623 573 491 319 281 236 201 177
90 36 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 373 3069
Size: 174 199 2696
Fragments arranged by size:
2696 199 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 137 480 980 1864 3069
Size: 137 343 500 884 1205
Fragments arranged by size:
1205 884 500 343 137
BsaAI yAC'GTr
Cuts at: 0 821 942 1704 1731 3069
Size: 821 121 762 27 1338
Fragments arranged by size:
1338 821 762 121 27
BsaBI GATnn'nnATC
Cuts at: 0 2666 3069
Size: 2666 403
BsaHI Gr'CG_yC
Cuts at: 0 780 2167 3069
Size: 780 1387 902
Fragments arranged by size:
1387 902 780
BsaWI w'CCGG_w
Cuts at: 0 2562 2835 3069
Size: 2562 273 234
Fragments arranged by size:
2562 273 234
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 910 1033 1036 1329 1683 1999 3069
Size: 307 603 123 3 293 354 316 1070
Fragments arranged by size:
1070 603 354 316 307 293 123 3
BseYI C'CCAG_C
Cuts at: 0 295 846 1116 1218 1236 1513 1640 2400
Size: 295 551 270 102 18 277 127 760
Cuts at: 2400 2531 2616 3069
Size: 131 85 453
Fragments arranged by size:
760 551 453 295 277 270 131 127
102 85 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1558 1753 2382 3069
Size: 1558 195 629 687
Fragments arranged by size:
1558 687 629 195
BsiHKAI G_wGCw'C
Cuts at: 0 659 2387 3069
Size: 659 1728 682
Fragments arranged by size:
1728 682 659
BsmI GAATG_Cn'
Cuts at: 0 1763 3069
Size: 1763 1306
Bsp1286I G_dGCh'C
Cuts at: 0 172 294 446 659 790 1128 1585 1628
Size: 172 122 152 213 131 338 457 43
Cuts at: 1628 1961 2339 2387 2531 2573 2655 2682 2871
Size: 333 378 48 144 42 82 27 189
Cuts at: 2871 3055 3069
Size: 184 14
Fragments arranged by size:
457 378 338 333 213 189 184 172
152 144 131 122 82 48 43 42
27 14
BspEI T'CCGG_A
Cuts at: 0 2835 3069
Size: 2835 234
BspHI T'CATG_A
Cuts at: 0 1664 3069
Size: 1664 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1862 1934 1958 2486 2801 3069
Size: 1862 72 24 528 315 268
Fragments arranged by size:
1862 528 315 268 72 24
BsrBI CCG'CTC
Cuts at: 0 2649 2918 3069
Size: 2649 269 151
Fragments arranged by size:
2649 269 151
BsrDI GCAATG_nn'
Cuts at: 0 2111 3069
Size: 2111 958
BsrFI r'CCGG_y
Cuts at: 0 308 559 1544 3069
Size: 308 251 985 1525
Fragments arranged by size:
1525 985 308 251
BssSI C'ACGA_G
Cuts at: 0 2798 3069
Size: 2798 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1776 1796 2577 3069
Size: 1776 20 781 492
Fragments arranged by size:
1776 781 492 20
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2604 3009 3069
Size: 2604 405 60
Fragments arranged by size:
2604 405 60
BstYI r'GATC_y
Cuts at: 0 1844 1997 2667 2832 3017 3069
Size: 1844 153 670 165 185 52
Fragments arranged by size:
1844 670 185 165 153 52
Bsu36I CC'TnA_GG
Cuts at: 0 722 2950 3069
Size: 722 2228 119
Fragments arranged by size:
2228 722 119
BtgI C'CryG_G
Cuts at: 0 1104 3002 3069
Size: 1104 1898 67
Fragments arranged by size:
1898 1104 67
BtsI GCAGTG_nn'
Cuts at: 0 88 573 1585 1750 2162 3069
Size: 88 485 1012 165 412 907
Fragments arranged by size:
1012 907 485 412 165 88
EarI CTCTTCn'nnn_
Cuts at: 0 65 71 1010 1193 2456 3069
Size: 65 6 939 183 1263 613
Fragments arranged by size:
1263 939 613 183 65 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 426 3069
Size: 426 2643
EcoICRI GAG'CTC
Cuts at: 0 2385 3069
Size: 2385 684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 899 919 1025 1154 1326 1393 1555
Size: 52 847 20 106 129 172 67 162
Cuts at: 1555 1978 3069
Size: 423 1091
Fragments arranged by size:
1091 847 423 172 162 129 106 67
52 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1576 2014 2214 3069
Size: 1576 438 200 855
Fragments arranged by size:
1576 855 438 200
EcoO109I rG'GnC_Cy
Cuts at: 0 143 343 1000 1209 1394 1451 1947 2254
Size: 143 200 657 209 185 57 496 307
Cuts at: 2254 2262 2292 2475 2527 2528 2652 3052 3069
Size: 8 30 183 52 1 124 400 17
Fragments arranged by size:
657 496 400 307 209 200 185 183
143 124 57 52 30 17 8 1
FspI TGC'GCA
Cuts at: 0 1789 3069
Size: 1789 1280
HaeII r_GCGC'y
Cuts at: 0 369 783 2170 2502 2787 3069
Size: 369 414 1387 332 285 282
Fragments arranged by size:
1387 414 369 332 285 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 702 734 958 990 1888 1920 2101 2133
Size: 702 32 224 32 898 32 181 32
Cuts at: 2133 2194 2226 3069
Size: 61 32 843
Fragments arranged by size:
898 843 702 224 181 61 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 779 2166 3069
Size: 779 1387 903
Fragments arranged by size:
1387 903 779
MslI CAynn'nnrTG
Cuts at: 0 244 641 1729 2186 2602 2996 3007 3069
Size: 244 397 1088 457 416 394 11 62
Fragments arranged by size:
1088 457 416 397 394 244 62 11
MspA1I CmG'CkG
Cuts at: 0 520 850 1174 2358 2380 2620 3049 3069
Size: 520 330 324 1184 22 240 429 20
Fragments arranged by size:
1184 520 429 330 324 240 22 20
NarI GG'CG_CC
Cuts at: 0 780 2167 3069
Size: 780 1387 902
Fragments arranged by size:
1387 902 780
NcoI C'CATG_G
Cuts at: 0 1104 3002 3069
Size: 1104 1898 67
Fragments arranged by size:
1898 1104 67
PasI CC'CwG_GG
Cuts at: 0 330 2289 3069
Size: 330 1959 780
Fragments arranged by size:
1959 780 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 542 1800 2346 2688 2829 3069
Size: 542 1258 546 342 141 240
Fragments arranged by size:
1258 546 542 342 240 141
PfoI T'CCnGG_A
Cuts at: 0 1840 1943 2471 2642 3020 3069
Size: 1840 103 528 171 378 49
Fragments arranged by size:
1840 528 378 171 103 49
PmlI CAC'GTG
Cuts at: 0 942 1731 3069
Size: 942 789 1338
Fragments arranged by size:
1338 942 789
PpuMI rG'GwC_Cy
Cuts at: 0 143 343 1394 1947 2262 2475 3069
Size: 143 200 1051 553 315 213 594
Fragments arranged by size:
1051 594 553 315 213 200 143
PspOMI G'GGCC_C
Cuts at: 0 1124 2527 2651 2678 3051 3069
Size: 1124 1403 124 27 373 18
Fragments arranged by size:
1403 1124 373 124 27 18
PspXI vC'TCGA_Gb
Cuts at: 0 300 3069
Size: 300 2769
PstI C_TGCA'G
Cuts at: 0 1474 2357 2436 3044 3069
Size: 1474 883 79 608 25
Fragments arranged by size:
1474 883 608 79 25
PvuII CAG'CTG
Cuts at: 0 520 850 1174 2358 2380 2620 3069
Size: 520 330 324 1184 22 240 449
Fragments arranged by size:
1184 520 449 330 324 240 22
SacI G_AGCT'C
Cuts at: 0 2387 3069
Size: 2387 682
SanDI GG'GwC_CC
Cuts at: 0 343 1394 3069
Size: 343 1051 1675
Fragments arranged by size:
1675 1051 343
SapI GCTCTTCn'nnn_
Cuts at: 0 71 3069
Size: 71 2998
SbfI CC_TGCA'GG
Cuts at: 0 1474 3044 3069
Size: 1474 1570 25
Fragments arranged by size:
1570 1474 25
SfcI C'TryA_G
Cuts at: 0 952 1470 2083 2353 2370 2432 3040 3069
Size: 952 518 613 270 17 62 608 29
Fragments arranged by size:
952 613 608 518 270 62 29 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 595 3069
Size: 595 2474
SfoI GGC'GCC
Cuts at: 0 781 2168 3069
Size: 781 1387 901
Fragments arranged by size:
1387 901 781
SmlI C'TyrA_G
Cuts at: 0 189 300 388 2103 3069
Size: 189 111 88 1715 966
Fragments arranged by size:
1715 966 189 111 88
StuI AGG'CCT
Cuts at: 0 589 598 3069
Size: 589 9 2471
Fragments arranged by size:
2471 589 9
StyI C'CwwG_G
Cuts at: 0 1104 2015 2257 3002 3069
Size: 1104 911 242 745 67
Fragments arranged by size:
1104 911 745 242 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 343 2302 3069
Size: 343 1959 767
Fragments arranged by size:
1959 767 343
TatI w'GTAC_w
Cuts at: 0 1616 3069
Size: 1616 1453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 109 141 1709 1741 3069
Size: 67 32 10 32 1568 32 1328
Fragments arranged by size:
1568 1328 67 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 0 1885 3069
Size: 1885 1184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 761 3069
Size: 761 2308
XhoI C'TCGA_G
Cuts at: 0 300 3069
Size: 300 2769
XmnI GAAnn'nnTTC
Cuts at: 0 2326 3069
Size: 2326 743
Enzymes that do cut:
AcuI AfeI AflII AflIII AleI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MslI MspA1I
NarI NcoI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SacI SanDI SapI SbfI SfcI
SfiI SfoI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI BaeI BciVI BfrBI
BmtI BsaXI BsiEI BsiWI BsmBI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI ScaI SexAI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XmaI ZraI