Restriction map for
pF1KSDA0193
(Linear) (Six-base) MAPSORT of: KIAA0193.seq Check: 4641 from: 1 to: 1242
KIAA0193.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 766 1242
Size: 462 304 476
Fragments arranged by size:
476 462 304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 879 911 991 1023 1216 1242
Size: 879 32 80 32 193 26
Fragments arranged by size:
879 193 80 32 32 26
ApaLI G'TGCA_C
Cuts at: 0 159 1242
Size: 159 1083
ApoI r'AATT_y
Cuts at: 0 78 1242
Size: 78 1164
AvaI C'yCGr_G
Cuts at: 0 502 1242
Size: 502 740
BbvCI CC'TCA_GC
Cuts at: 0 1102 1242
Size: 1102 140
BclI T'GATC_A
Cuts at: 0 132 1242
Size: 132 1110
Bme1580I G_kGCm'C
Cuts at: 0 163 1062 1242
Size: 163 899 180
Fragments arranged by size:
899 180 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1242
Size: 531 711
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 638 1242
Size: 638 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1138 1144 1242
Size: 665 473 6 98
Fragments arranged by size:
665 473 98 6
Bpu10I CC'TnA_GC
Cuts at: 0 50 1102 1242
Size: 50 1052 140
Fragments arranged by size:
1052 140 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 1242
Size: 499 743
BsaAI yAC'GTr
Cuts at: 0 46 1063 1242
Size: 46 1017 179
Fragments arranged by size:
1017 179 46
BsaWI w'CCGG_w
Cuts at: 0 141 603 1242
Size: 141 462 639
Fragments arranged by size:
639 462 141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 824 854 948 978 991 1021 1216 1242
Size: 824 30 94 30 13 30 195 26
Fragments arranged by size:
824 195 94 30 30 30 26 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2 1119 1242
Size: 2 1117 123
Fragments arranged by size:
1117 123 2
BseYI C'CCAG_C
Cuts at: 0 321 1242
Size: 321 921
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1242
Size: 772 470
BsiHKAI G_wGCw'C
Cuts at: 0 163 503 1111 1242
Size: 163 340 608 131
Fragments arranged by size:
608 340 163 131
BsmI GAATG_Cn'
Cuts at: 0 869 1242
Size: 869 373
Bsp1286I G_dGCh'C
Cuts at: 0 163 503 1062 1111 1242
Size: 163 340 559 49 131
Fragments arranged by size:
559 340 163 131 49
BspEI T'CCGG_A
Cuts at: 0 603 1242
Size: 603 639
BsrFI r'CCGG_y
Cuts at: 0 1028 1242
Size: 1028 214
BsrGI T'GTAC_A
Cuts at: 0 1040 1242
Size: 1040 202
BssSI C'ACGA_G
Cuts at: 0 1051 1242
Size: 1051 191
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 1242
Size: 588 654
BstYI r'GATC_y
Cuts at: 0 330 1242
Size: 330 912
BtgI C'CryG_G
Cuts at: 0 1145 1242
Size: 1145 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 619 654 1242
Size: 619 35 588
Fragments arranged by size:
619 588 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1138 1144 1242
Size: 665 473 6 98
Fragments arranged by size:
665 473 98 6
EcoO109I rG'GnC_Cy
Cuts at: 0 191 1197 1242
Size: 191 1006 45
Fragments arranged by size:
1006 191 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 465 497 824 856 1242
Size: 427 32 6 32 327 32 386
Fragments arranged by size:
427 386 327 32 32 32 6
HincII GTy'rAC
Cuts at: 0 1218 1242
Size: 1218 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1242
Size: 380 862
MslI CAynn'nnrTG
Cuts at: 0 586 748 1242
Size: 586 162 494
Fragments arranged by size:
586 494 162
MspA1I CmG'CkG
Cuts at: 0 301 788 1242
Size: 301 487 454
Fragments arranged by size:
487 454 301
NaeI GCC'GGC
Cuts at: 0 1030 1242
Size: 1030 212
NcoI C'CATG_G
Cuts at: 0 1145 1242
Size: 1145 97
NgoMIV G'CCGG_C
Cuts at: 0 1028 1242
Size: 1028 214
PfoI T'CCnGG_A
Cuts at: 0 1010 1242
Size: 1010 232
PmlI CAC'GTG
Cuts at: 0 46 1063 1242
Size: 46 1017 179
Fragments arranged by size:
1017 179 46
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 991 1023 1216 1242
Size: 640 32 319 32 193 26
Fragments arranged by size:
640 319 193 32 32 26
PpuMI rG'GwC_Cy
Cuts at: 0 191 1197 1242
Size: 191 1006 45
Fragments arranged by size:
1006 191 45
PstI C_TGCA'G
Cuts at: 0 9 1242
Size: 9 1233
PvuII CAG'CTG
Cuts at: 0 301 1242
Size: 301 941
ScaI AGT'ACT
Cuts at: 0 520 1242
Size: 520 722
SfcI C'TryA_G
Cuts at: 0 5 1242
Size: 5 1237
SmlI C'TyrA_G
Cuts at: 0 502 1242
Size: 502 740
StuI AGG'CCT
Cuts at: 0 1136 1242
Size: 1136 106
StyI C'CwwG_G
Cuts at: 0 187 630 1145 1242
Size: 187 443 515 97
Fragments arranged by size:
515 443 187 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1242
Size: 486 756
TatI w'GTAC_w
Cuts at: 0 518 1040 1242
Size: 518 522 202
Fragments arranged by size:
522 518 202
XbaI T'CTAG_A
Cuts at: 0 696 1242
Size: 696 546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1119 1242
Size: 1119 123
XhoI C'TCGA_G
Cuts at: 0 502 1242
Size: 502 740
Enzymes that do cut:
AlfI AloI ApaLI ApoI AvaI BbvCI BclI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrFI
BsrGI BssSI BstXI BstYI BtgI CspCI Eco57MI EcoO109I
Hin4I HincII MmeI MslI MspA1I NaeI NcoI NgoMIV
PfoI PmlI PpiI PpuMI PstI PvuII ScaI SfcI
SmlI StuI StyI TaqII TatI XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BpuEI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BspMI
BsrBI BsrDI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XmaI XmnI ZraI