Restriction map for
pF1KSDA0194
(Linear) (Six-base) MAPSORT of: KIAA0194.seq Check: 1573 from: 1 to: 3876
KIAA0194.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2802 2998 3047 3876
Size: 2802 196 49 829
Fragments arranged by size:
2802 829 196 49
AccI GT'mk_AC
Cuts at: 0 1895 3876
Size: 1895 1981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1687 1702 2542 2737 3876
Size: 1687 15 840 195 1139
Fragments arranged by size:
1687 1139 840 195 15
AhdI GACnn_n'nnGTC
Cuts at: 0 1661 3876
Size: 1661 2215
AleI CACnn'nnGTG
Cuts at: 0 993 3313 3876
Size: 993 2320 563
Fragments arranged by size:
2320 993 563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 558 1618 2104 3876
Size: 558 1060 486 1772
Fragments arranged by size:
1772 1060 558 486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 880 912 3876
Size: 451 32 397 32 2964
Fragments arranged by size:
2964 451 397 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 546 598 1200 2076 2344 2586 2947 3039
Size: 546 52 602 876 268 242 361 92
Cuts at: 3039 3466 3876
Size: 427 410
Fragments arranged by size:
876 602 546 427 410 361 268 242
92 52
ApaI G_GGCC'C
Cuts at: 0 2300 3876
Size: 2300 1576
ApoI r'AATT_y
Cuts at: 0 1137 1176 1919 2743 2845 3876
Size: 1137 39 743 824 102 1031
Fragments arranged by size:
1137 1031 824 743 102 39
AvaI C'yCGr_G
Cuts at: 0 1291 3876
Size: 1291 2585
AvrII C'CTAG_G
Cuts at: 0 1017 3876
Size: 1017 2859
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 11 44 1295 1328 3876
Size: 11 33 1251 33 2548
Fragments arranged by size:
2548 1251 33 33 11
BamHI G'GATC_C
Cuts at: 0 306 1031 3876
Size: 306 725 2845
Fragments arranged by size:
2845 725 306
BanI G'GyrC_C
Cuts at: 0 2986 3560 3591 3876
Size: 2986 574 31 285
Fragments arranged by size:
2986 574 285 31
BanII G_rGCy'C
Cuts at: 0 191 582 1491 1993 2027 2300 2755 3431
Size: 191 391 909 502 34 273 455 676
Cuts at: 3431 3876
Size: 445
Fragments arranged by size:
909 676 502 455 445 391 273 191
34
BbsI GAAGACnn'nnnn_
Cuts at: 0 2118 2554 3080 3876
Size: 2118 436 526 796
Fragments arranged by size:
2118 796 526 436
BbvCI CC'TCA_GC
Cuts at: 0 434 566 628 2120 3876
Size: 434 132 62 1492 1756
Fragments arranged by size:
1756 1492 434 132 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1897 1931 3876
Size: 1897 34 1945
Fragments arranged by size:
1945 1897 34
BclI T'GATC_A
Cuts at: 0 1253 3876
Size: 1253 2623
BglI GCCn_nnn'nGGC
Cuts at: 0 527 1618 3868 3876
Size: 527 1091 2250 8
Fragments arranged by size:
2250 1091 527 8
BlpI GC'TnA_GC
Cuts at: 0 2569 3876
Size: 2569 1307
Bme1580I G_kGCm'C
Cuts at: 0 518 764 1000 2300 2989 3193 3243 3563
Size: 518 246 236 1300 689 204 50 320
Cuts at: 3563 3583 3876
Size: 20 293
Fragments arranged by size:
1300 689 518 320 293 246 236 204
50 20
BmgBI CAC'GTC
Cuts at: 0 3157 3311 3876
Size: 3157 154 565
Fragments arranged by size:
3157 565 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 582 1564 3481 3545 3876
Size: 582 982 1917 64 331
Fragments arranged by size:
1917 982 582 331 64
BmtI G_CTAG'C
Cuts at: 0 2713 3876
Size: 2713 1163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 449 843 2007 2223 3027 3876
Size: 449 394 1164 216 804 849
Fragments arranged by size:
1164 849 804 449 394 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1406 1410 1475 2962 3876
Size: 1192 214 4 65 1487 914
Fragments arranged by size:
1487 1192 914 214 65 4
Bpu10I CC'TnA_GC
Cuts at: 0 434 566 628 2029 2120 3043 3876
Size: 434 132 62 1401 91 923 833
Fragments arranged by size:
1401 923 833 434 132 91 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 2419 3876
Size: 706 1713 1457
Fragments arranged by size:
1713 1457 706
BsaHI Gr'CG_yC
Cuts at: 0 1079 1321 3876
Size: 1079 242 2555
Fragments arranged by size:
2555 1079 242
BsaWI w'CCGG_w
Cuts at: 0 345 422 1955 3876
Size: 345 77 1533 1921
Fragments arranged by size:
1921 1533 345 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 722 752 880 910 1225 1255 1379 1409
Size: 722 30 128 30 315 30 124 30
Cuts at: 1409 2575 2605 2885 2915 3876
Size: 1166 30 280 30 961
Fragments arranged by size:
1166 961 722 315 280 128 124 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 276 473 583 819 1202 1246 2197 2578
Size: 276 197 110 236 383 44 951 381
Cuts at: 2578 2706 2713 2906 3850 3853 3868 3876
Size: 128 7 193 944 3 15 8
Fragments arranged by size:
951 944 383 381 276 236 197 193
128 110 44 15 8 7 3
BseYI C'CCAG_C
Cuts at: 0 517 1689 1927 2293 3556 3876
Size: 517 1172 238 366 1263 320
Fragments arranged by size:
1263 1172 517 366 320 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 311 818 1363 1803 2371 2551 2956
Size: 199 112 507 545 440 568 180 405
Cuts at: 2956 3876
Size: 920
Fragments arranged by size:
920 568 545 507 440 405 199 180
112
BsiHKAI G_wGCw'C
Cuts at: 0 191 644 961 1491 1512 1993 2035 3431
Size: 191 453 317 530 21 481 42 1396
Cuts at: 3431 3473 3876
Size: 42 403
Fragments arranged by size:
1396 530 481 453 403 317 191 42
42 21
BsmI GAATG_Cn'
Cuts at: 0 2019 3876
Size: 2019 1857
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1086 3876
Size: 1086 2790
Bsp1286I G_dGCh'C
Cuts at: 0 191 518 582 644 764 961 1000 1491
Size: 191 327 64 62 120 197 39 491
Cuts at: 1491 1512 1993 2027 2035 2300 2755 2989 3193
Size: 21 481 34 8 265 455 234 204
Cuts at: 3193 3243 3431 3473 3563 3583 3876
Size: 50 188 42 90 20 293
Fragments arranged by size:
491 481 455 327 293 265 234 204
197 191 188 120 90 64 62 50
42 39 34 21 20 8
BspEI T'CCGG_A
Cuts at: 0 345 3876
Size: 345 3531
BspHI T'CATG_A
Cuts at: 0 803 2139 2885 3876
Size: 803 1336 746 991
Fragments arranged by size:
1336 991 803 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1475 2802 2998 3047 3266 3876
Size: 1475 1327 196 49 219 610
Fragments arranged by size:
1475 1327 610 219 196 49
BsrDI GCAATG_nn'
Cuts at: 0 2601 3656 3876
Size: 2601 1055 220
Fragments arranged by size:
2601 1055 220
BsrGI T'GTAC_A
Cuts at: 0 600 3123 3753 3876
Size: 600 2523 630 123
Fragments arranged by size:
2523 630 600 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 334 2076 2586 2824 3039 3876
Size: 334 1742 510 238 215 837
Fragments arranged by size:
1742 837 510 334 238 215
BstEII G'GTnAC_C
Cuts at: 0 3544 3876
Size: 3544 332
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3315 3876
Size: 3315 561
BstYI r'GATC_y
Cuts at: 0 306 368 623 1031 2048 3740 3876
Size: 306 62 255 408 1017 1692 136
Fragments arranged by size:
1692 1017 408 306 255 136 62
Bsu36I CC'TnA_GG
Cuts at: 0 1349 3876
Size: 1349 2527
BtgI C'CryG_G
Cuts at: 0 933 3876
Size: 933 2943
BtsI GCAGTG_nn'
Cuts at: 0 497 547 2065 2248 2958 2964 3055 3872
Size: 497 50 1518 183 710 6 91 817
Cuts at: 3872 3876
Size: 4
Fragments arranged by size:
1518 817 710 497 183 91 50 6
4
DraIII CAC_nnn'GTG
Cuts at: 0 2243 3876
Size: 2243 1633
EaeI y'GGCC_r
Cuts at: 0 254 3280 3305 3860 3876
Size: 254 3026 25 555 16
Fragments arranged by size:
3026 555 254 25 16
EarI CTCTTCn'nnn_
Cuts at: 0 704 1089 2713 3017 3273 3845 3876
Size: 704 385 1624 304 256 572 31
Fragments arranged by size:
1624 704 572 385 304 256 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3634 3876
Size: 3634 242
EcoICRI GAG'CTC
Cuts at: 0 189 1489 1991 3429 3876
Size: 189 1300 502 1438 447
Fragments arranged by size:
1438 1300 502 447 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1406 1410 1475 1687 1702 2542 2737
Size: 1192 214 4 65 212 15 840 195
Cuts at: 2737 2962 3876
Size: 225 914
Fragments arranged by size:
1192 914 840 225 214 212 195 65
15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 415 565 631 1650 2967 3876
Size: 415 150 66 1019 1317 909
Fragments arranged by size:
1317 1019 909 415 150 66
EcoO109I rG'GnC_Cy
Cuts at: 0 478 558 2297 2493 3876
Size: 478 80 1739 196 1383
Fragments arranged by size:
1739 1383 478 196 80
EcoRI G'AATT_C
Cuts at: 0 1176 2743 3876
Size: 1176 1567 1133
Fragments arranged by size:
1567 1176 1133
FspI TGC'GCA
Cuts at: 0 2080 3876
Size: 2080 1796
HaeII r_GCGC'y
Cuts at: 0 2058 3876
Size: 2058 1818
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 772 804 1647 1679 2092 2124
Size: 298 32 442 32 843 32 413 32
Cuts at: 2124 2218 2250 2492 2524 2885 2917 3876
Size: 94 32 242 32 361 32 959
Fragments arranged by size:
959 843 442 413 361 298 242 94
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1464 1575 1941 2907 3876
Size: 1464 111 366 966 969
Fragments arranged by size:
1464 969 966 366 111
HindIII A'AGCT_T
Cuts at: 0 2995 3876
Size: 2995 881
HpaI GTT'AAC
Cuts at: 0 1575 1941 3876
Size: 1575 366 1935
Fragments arranged by size:
1935 1575 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 518 1775 2195 3876
Size: 217 301 1257 420 1681
Fragments arranged by size:
1681 1257 420 301 217
MscI TGG'CCA
Cuts at: 0 3307 3876
Size: 3307 569
MslI CAynn'nnrTG
Cuts at: 0 993 3313 3876
Size: 993 2320 563
Fragments arranged by size:
2320 993 563
MspA1I CmG'CkG
Cuts at: 0 1689 1817 1931 2073 2577 3651 3876
Size: 1689 128 114 142 504 1074 225
Fragments arranged by size:
1689 1074 504 225 142 128 114
NcoI C'CATG_G
Cuts at: 0 933 3876
Size: 933 2943
NdeI CA'TA_TG
Cuts at: 0 12 969 3876
Size: 12 957 2907
Fragments arranged by size:
2907 957 12
NheI G'CTAG_C
Cuts at: 0 2709 3876
Size: 2709 1167
NspI r_CATG'y
Cuts at: 0 944 3876
Size: 944 2932
PasI CC'CwG_GG
Cuts at: 0 952 1477 1607 3876
Size: 952 525 130 2269
Fragments arranged by size:
2269 952 525 130
PflMI CCAn_nnn'nTGG
Cuts at: 0 1275 2379 2947 3201 3570 3876
Size: 1275 1104 568 254 369 306
Fragments arranged by size:
1275 1104 568 369 306 254
PfoI T'CCnGG_A
Cuts at: 0 1383 2978 3876
Size: 1383 1595 898
Fragments arranged by size:
1595 1383 898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 880 912 3876
Size: 880 32 2964
Fragments arranged by size:
2964 880 32
PpuMI rG'GwC_Cy
Cuts at: 0 478 2493 3876
Size: 478 2015 1383
Fragments arranged by size:
2015 1383 478
PshAI GACnn'nnGTC
Cuts at: 0 1324 3876
Size: 1324 2552
PspOMI G'GGCC_C
Cuts at: 0 2296 3876
Size: 2296 1580
PstI C_TGCA'G
Cuts at: 0 416 1539 2072 2093 2336 2582 3035 3876
Size: 416 1123 533 21 243 246 453 841
Fragments arranged by size:
1123 841 533 453 416 246 243 21
PvuII CAG'CTG
Cuts at: 0 1689 1817 1931 2073 2577 3876
Size: 1689 128 114 142 504 1299
Fragments arranged by size:
1689 1299 504 142 128 114
SacI G_AGCT'C
Cuts at: 0 191 1491 1993 3431 3876
Size: 191 1300 502 1438 445
Fragments arranged by size:
1438 1300 502 445 191
SapI GCTCTTCn'nnn_
Cuts at: 0 2713 3876
Size: 2713 1163
SbfI CC_TGCA'GG
Cuts at: 0 416 3876
Size: 416 3460
SfcI C'TryA_G
Cuts at: 0 412 1454 1535 2068 2089 2253 2332 2578
Size: 412 1042 81 533 21 164 79 246
Cuts at: 2578 3015 3031 3529 3876
Size: 437 16 498 347
Fragments arranged by size:
1042 533 498 437 412 347 246 164
81 79 21 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1618 3876
Size: 1618 2258
SmaI CCC'GGG
Cuts at: 0 1293 3876
Size: 1293 2583
StuI AGG'CCT
Cuts at: 0 1621 3527 3876
Size: 1621 1906 349
Fragments arranged by size:
1906 1621 349
StyI C'CwwG_G
Cuts at: 0 716 933 1017 1973 2963 3080 3569 3804
Size: 716 217 84 956 990 117 489 235
Cuts at: 3804 3876
Size: 72
Fragments arranged by size:
990 956 716 489 235 217 117 84
72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 99 3500 3876
Size: 99 3401 376
Fragments arranged by size:
3401 376 99
TatI w'GTAC_w
Cuts at: 0 152 600 1811 2007 3123 3753 3876
Size: 152 448 1211 196 1116 630 123
Fragments arranged by size:
1211 1116 630 448 196 152 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 722 754 1225 1257 2575 2607 3876
Size: 722 32 471 32 1318 32 1269
Fragments arranged by size:
1318 1269 722 471 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 772 1059 1095 1167 2679 3876
Size: 750 22 287 36 72 1512 1197
Fragments arranged by size:
1512 1197 750 287 72 36 22
XmaI C'CCGG_G
Cuts at: 0 1291 3876
Size: 1291 2585
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AloI AlwNI
ApaI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI
NdeI NheI NspI PasI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SapI SbfI SfcI
SfiI SmaI StuI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI ApaLI
AscI AseI AsiSI BbeI BciVI BfrBI BglII BpuEI
BsaAI BsaBI BsiEI BsiWI BsrBI BsrFI BssHII BssSI
BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EcoRV FalI FseI FspAI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NotI NruI NsiI PacI PciI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI ScaI SexAI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI ZraI