Restriction map for
pF1KSDA0195
(Linear) (Six-base) MAPSORT of: KIAA0195.seq Check: 3052 from: 1 to: 4068
KIAA0195.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2891 4068
Size: 2891 1177
AccI GT'mk_AC
Cuts at: 0 2555 4068
Size: 2555 1513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 97 376 888 1287 1539 4068
Size: 28 69 279 512 399 252 2529
Fragments arranged by size:
2529 512 399 279 252 69 28
AhdI GACnn_n'nnGTC
Cuts at: 0 520 4068
Size: 520 3548
AleI CACnn'nnGTG
Cuts at: 0 3751 4068
Size: 3751 317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3430 4068
Size: 3430 638
AlwNI CAG_nnn'CTG
Cuts at: 0 799 1291 2100 2393 2451 3545 3820 4029
Size: 799 492 809 293 58 1094 275 209
Cuts at: 4029 4068
Size: 39
Fragments arranged by size:
1094 809 799 492 293 275 209 58
39
AvaI C'yCGr_G
Cuts at: 0 677 912 1168 1381 1489 2175 2732 2855
Size: 677 235 256 213 108 686 557 123
Cuts at: 2855 3593 4068
Size: 738 475
Fragments arranged by size:
738 686 677 557 475 256 235 213
123 108
BamHI G'GATC_C
Cuts at: 0 1259 3063 4068
Size: 1259 1804 1005
Fragments arranged by size:
1804 1259 1005
BanI G'GyrC_C
Cuts at: 0 767 2110 2313 4068
Size: 767 1343 203 1755
Fragments arranged by size:
1755 1343 767 203
BanII G_rGCy'C
Cuts at: 0 709 1225 1322 1388 1496 1589 2401 2579
Size: 709 516 97 66 108 93 812 178
Cuts at: 2579 2753 3654 3787 4068
Size: 174 901 133 281
Fragments arranged by size:
901 812 709 516 281 178 174 133
108 97 93 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 2441 3493 3726 4068
Size: 2441 1052 233 342
Fragments arranged by size:
2441 1052 342 233
BbvCI CC'TCA_GC
Cuts at: 0 36 67 2044 4068
Size: 36 31 1977 2024
Fragments arranged by size:
2024 1977 36 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2280 2314 4068
Size: 2280 34 1754
Fragments arranged by size:
2280 1754 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 464 1083 2872 4068
Size: 464 619 1789 1196
Fragments arranged by size:
1789 1196 619 464
BclI T'GATC_A
Cuts at: 0 2054 4068
Size: 2054 2014
BglI GCCn_nnn'nGGC
Cuts at: 0 1408 1910 2699 4068
Size: 1408 502 789 1369
Fragments arranged by size:
1408 1369 789 502
BglII A'GATC_T
Cuts at: 0 2462 4068
Size: 2462 1606
BlpI GC'TnA_GC
Cuts at: 0 1189 1666 3403 4068
Size: 1189 477 1737 665
Fragments arranged by size:
1737 1189 665 477
Bme1580I G_kGCm'C
Cuts at: 0 857 912 1880 2318 2906 2918 3917 4068
Size: 857 55 968 438 588 12 999 151
Fragments arranged by size:
999 968 857 588 438 151 55 12
BmgBI CAC'GTC
Cuts at: 0 137 3891 3912 4068
Size: 137 3754 21 156
Fragments arranged by size:
3754 156 137 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 507 1970 2481 4068
Size: 437 70 1463 511 1587
Fragments arranged by size:
1587 1463 511 437 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 51 1134 1420 2793 4068
Size: 51 1083 286 1373 1275
Fragments arranged by size:
1373 1275 1083 286 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 652 928 934 1678 2415 2446 2632
Size: 109 543 276 6 744 737 31 186
Cuts at: 2632 2667 2848 3022 3454 3638 3925 4068
Size: 35 181 174 432 184 287 143
Fragments arranged by size:
744 737 543 432 287 276 186 184
181 174 143 109 35 31 6
Bpu10I CC'TnA_GC
Cuts at: 0 36 67 2044 2452 4068
Size: 36 31 1977 408 1616
Fragments arranged by size:
1977 1616 408 36 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 3505 4068
Size: 757 2748 563
Fragments arranged by size:
2748 757 563
BsaI GGTCTCn'nnnn_
Cuts at: 0 635 733 2935 3088 3205 4068
Size: 635 98 2202 153 117 863
Fragments arranged by size:
2202 863 635 153 117 98
BsaBI GATnn'nnATC
Cuts at: 0 2962 4068
Size: 2962 1106
BsaHI Gr'CG_yC
Cuts at: 0 962 1400 4068
Size: 962 438 2668
Fragments arranged by size:
2668 962 438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 421 451 1723 1753 2686 2716
Size: 169 30 222 30 1272 30 933 30
Cuts at: 2716 3604 3634 4068
Size: 888 30 434
Fragments arranged by size:
1272 933 888 434 222 169 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 1110 2743 2746 2779 2782 3223 3609
Size: 931 179 1633 3 33 3 441 386
Cuts at: 3609 4068
Size: 459
Fragments arranged by size:
1633 931 459 441 386 179 33 3
3
BseYI C'CCAG_C
Cuts at: 0 240 710 1060 1279 1588 1598 2004 2353
Size: 240 470 350 219 309 10 406 349
Cuts at: 2353 2689 2818 3417 3491 3634 3831 4047 4068
Size: 336 129 599 74 143 197 216 21
Fragments arranged by size:
599 470 406 350 349 336 309 240
219 216 197 143 129 74 21 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1195 1417 1714 2121 2916 3709 4068
Size: 847 348 222 297 407 795 793 359
Fragments arranged by size:
847 795 793 407 359 348 297 222
BsiEI CG_ry'CG
Cuts at: 0 3710 4068
Size: 3710 358
BsiHKAI G_wGCw'C
Cuts at: 0 335 623 1169 2401 2579 4068
Size: 335 288 546 1232 178 1489
Fragments arranged by size:
1489 1232 546 335 288 178
BsmI GAATG_Cn'
Cuts at: 0 297 4068
Size: 297 3771
BsmBI CGTCTCn'nnnn_
Cuts at: 0 503 1391 4068
Size: 503 888 2677
Fragments arranged by size:
2677 888 503
Bsp1286I G_dGCh'C
Cuts at: 0 335 623 709 857 912 1169 1225 1322
Size: 335 288 86 148 55 257 56 97
Cuts at: 1322 1388 1496 1589 1880 2318 2401 2579 2753
Size: 66 108 93 291 438 83 178 174
Cuts at: 2753 2906 2918 3654 3787 3917 4068
Size: 153 12 736 133 130 151
Fragments arranged by size:
736 438 335 291 288 257 178 174
153 151 148 133 130 108 97 93
86 83 66 56 55 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 2891 2998 3023 3869 4068
Size: 536 2355 107 25 846 199
Fragments arranged by size:
2355 846 536 199 107 25
BsrBI CCG'CTC
Cuts at: 0 120 375 816 1367 2456 4068
Size: 120 255 441 551 1089 1612
Fragments arranged by size:
1612 1089 551 441 255 120
BsrDI GCAATG_nn'
Cuts at: 0 1832 3053 4068
Size: 1832 1221 1015
Fragments arranged by size:
1832 1221 1015
BsrFI r'CCGG_y
Cuts at: 0 350 719 868 4068
Size: 350 369 149 3200
Fragments arranged by size:
3200 369 350 149
BstEII G'GTnAC_C
Cuts at: 0 2985 3963 4068
Size: 2985 978 105
Fragments arranged by size:
2985 978 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 958 998 2826 4068
Size: 958 40 1828 1242
Fragments arranged by size:
1828 1242 958 40
BstYI r'GATC_y
Cuts at: 0 431 1259 2179 2462 2672 3063 4068
Size: 431 828 920 283 210 391 1005
Fragments arranged by size:
1005 920 828 431 391 283 210
Bsu36I CC'TnA_GG
Cuts at: 0 415 1159 4068
Size: 415 744 2909
Fragments arranged by size:
2909 744 415
BtgI C'CryG_G
Cuts at: 0 273 857 2106 4068
Size: 273 584 1249 1962
Fragments arranged by size:
1962 1249 584 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1163 1985 3742 4068
Size: 1163 822 1757 326
Fragments arranged by size:
1757 1163 822 326
BtsI GCAGTG_nn'
Cuts at: 0 101 797 3298 3568 4068
Size: 101 696 2501 270 500
Fragments arranged by size:
2501 696 500 270 101
DraIII CAC_nnn'GTG
Cuts at: 0 2342 2871 4068
Size: 2342 529 1197
Fragments arranged by size:
2342 1197 529
DrdI GACnn_nn'nnGTC
Cuts at: 0 3889 4068
Size: 3889 179
EaeI y'GGCC_r
Cuts at: 0 199 224 576 866 1270 1518 2319 2457
Size: 199 25 352 290 404 248 801 138
Cuts at: 2457 3707 4068
Size: 1250 361
Fragments arranged by size:
1250 801 404 361 352 290 248 199
138 25
EagI C'GGCC_G
Cuts at: 0 3707 4068
Size: 3707 361
EarI CTCTTCn'nnn_
Cuts at: 0 652 1577 2068 3037 3286 4068
Size: 652 925 491 969 249 782
Fragments arranged by size:
969 925 782 652 491 249
EcoICRI GAG'CTC
Cuts at: 0 2399 2577 4068
Size: 2399 178 1491
Fragments arranged by size:
2399 1491 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 97 109 376 652 888 928 934
Size: 28 69 12 267 276 236 40 6
Cuts at: 934 1287 1539 1678 2415 2446 2632 2667 2848
Size: 353 252 139 737 31 186 35 181
Cuts at: 2848 3022 3454 3638 3925 4068
Size: 174 432 184 287 143
Fragments arranged by size:
737 432 353 287 276 267 252 236
186 184 181 174 143 139 69 40
35 31 28 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1675 2722 2761 4068
Size: 1675 1047 39 1307
Fragments arranged by size:
1675 1307 1047 39
EcoO109I rG'GnC_Cy
Cuts at: 0 63 206 1341 1884 2448 4068
Size: 63 143 1135 543 564 1620
Fragments arranged by size:
1620 1135 564 543 143 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3804 4068
Size: 3804 264
HaeII r_GCGC'y
Cuts at: 0 1803 1859 1963 4068
Size: 1803 56 104 2105
Fragments arranged by size:
2105 1803 104 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 1342 1374 2147 2179 4068
Size: 506 32 804 32 773 32 1889
Fragments arranged by size:
1889 804 773 506 32 32 32
HincII GTy'rAC
Cuts at: 0 525 2535 4068
Size: 525 2010 1533
Fragments arranged by size:
2010 1533 525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1568 1742 1841 2861 4068
Size: 1568 174 99 1020 1207
Fragments arranged by size:
1568 1207 1020 174 99
MscI TGG'CCA
Cuts at: 0 578 1272 4068
Size: 578 694 2796
Fragments arranged by size:
2796 694 578
MslI CAynn'nnrTG
Cuts at: 0 2473 3121 3448 3751 4068
Size: 2473 648 327 303 317
Fragments arranged by size:
2473 648 327 317 303
MspA1I CmG'CkG
Cuts at: 0 87 182 1307 1728 2116 2393 3026 3159
Size: 87 95 1125 421 388 277 633 133
Cuts at: 3159 3300 3342 3867 4068
Size: 141 42 525 201
Fragments arranged by size:
1125 633 525 421 388 277 201 141
133 95 87 42
NaeI GCC'GGC
Cuts at: 0 870 4068
Size: 870 3198
NcoI C'CATG_G
Cuts at: 0 2106 4068
Size: 2106 1962
NgoMIV G'CCGG_C
Cuts at: 0 868 4068
Size: 868 3200
NspI r_CATG'y
Cuts at: 0 471 2445 2661 3253 4068
Size: 471 1974 216 592 815
Fragments arranged by size:
1974 815 592 471 216
PasI CC'CwG_GG
Cuts at: 0 42 1752 1880 4068
Size: 42 1710 128 2188
Fragments arranged by size:
2188 1710 128 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 3800 4068
Size: 3800 268
PfoI T'CCnGG_A
Cuts at: 0 629 2825 4068
Size: 629 2196 1243
Fragments arranged by size:
2196 1243 629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 1214 4068
Size: 1182 32 2854
Fragments arranged by size:
2854 1182 32
PpuMI rG'GwC_Cy
Cuts at: 0 1341 4068
Size: 1341 2727
PshAI GACnn'nnGTC
Cuts at: 0 3687 4068
Size: 3687 381
PstI C_TGCA'G
Cuts at: 0 2888 3158 3293 3575 3866 4034 4068
Size: 2888 270 135 282 291 168 34
Fragments arranged by size:
2888 291 282 270 168 135 34
PvuII CAG'CTG
Cuts at: 0 87 1728 2393 3026 3159 3300 3342 3867
Size: 87 1641 665 633 133 141 42 525
Cuts at: 3867 4068
Size: 201
Fragments arranged by size:
1641 665 633 525 201 141 133 87
42
SacI G_AGCT'C
Cuts at: 0 2401 2579 4068
Size: 2401 178 1489
Fragments arranged by size:
2401 1489 178
SapI GCTCTTCn'nnn_
Cuts at: 0 1577 4068
Size: 1577 2491
SexAI A'CCwGG_T
Cuts at: 0 518 4068
Size: 518 3550
SfcI C'TryA_G
Cuts at: 0 504 2884 3154 3289 3571 3862 4030 4068
Size: 504 2380 270 135 282 291 168 38
Fragments arranged by size:
2380 504 291 282 270 168 135 38
SmaI CCC'GGG
Cuts at: 0 679 914 2857 3595 4068
Size: 679 235 1943 738 473
Fragments arranged by size:
1943 738 679 473 235
SmlI C'TyrA_G
Cuts at: 0 736 3520 4068
Size: 736 2784 548
Fragments arranged by size:
2784 736 548
SphI G_CATG'C
Cuts at: 0 2445 3253 4068
Size: 2445 808 815
Fragments arranged by size:
2445 815 808
StuI AGG'CCT
Cuts at: 0 573 1054 2134 3133 4068
Size: 573 481 1080 999 935
Fragments arranged by size:
1080 999 935 573 481
StyI C'CwwG_G
Cuts at: 0 1928 2106 4068
Size: 1928 178 1962
Fragments arranged by size:
1962 1928 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1074 1547 2661 3763 4068
Size: 1074 473 1114 1102 305
Fragments arranged by size:
1114 1102 1074 473 305
TatI w'GTAC_w
Cuts at: 0 932 1293 4068
Size: 932 361 2775
Fragments arranged by size:
2775 932 361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 453 1627 1659 1723 1755 2686 2718
Size: 421 32 1174 32 64 32 931 32
Cuts at: 2718 4068
Size: 1350
Fragments arranged by size:
1350 1174 931 421 64 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1068 1513 3120 3136 3447 3895 4068
Size: 1068 445 1607 16 311 448 173
Fragments arranged by size:
1607 1068 448 445 311 173 16
XmaI C'CCGG_G
Cuts at: 0 677 912 2855 3593 4068
Size: 677 235 1943 738 475
Fragments arranged by size:
1943 738 677 475 235
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AlwNI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PshAI PstI PvuII SacI SapI
SexAI SfcI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BfrBI BmtI BsaAI BsaWI BsiWI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI
DraI EciI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI