Restriction map for
pF1KA0196
(Linear) (Six-base) MAPSORT of: KIAA0196.seq Check: 5896 from: 1 to: 3477
KIAA0196.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 320 681 2466 3477
Size: 320 361 1785 1011
Fragments arranged by size:
1785 1011 361 320
AclI AA'CG_TT
Cuts at: 0 2455 3477
Size: 2455 1022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1915 2072 2320 3271 3449 3477
Size: 1915 157 248 951 178 28
Fragments arranged by size:
1915 951 248 178 157 28
AfeI AGC'GCT
Cuts at: 0 3103 3477
Size: 3103 374
AflIII A'CryG_T
Cuts at: 0 2509 3435 3477
Size: 2509 926 42
Fragments arranged by size:
2509 926 42
AlwNI CAG_nnn'CTG
Cuts at: 0 1239 2115 2868 3285 3477
Size: 1239 876 753 417 192
Fragments arranged by size:
1239 876 753 417 192
ApoI r'AATT_y
Cuts at: 0 247 1528 2667 2772 2783 3350 3477
Size: 247 1281 1139 105 11 567 127
Fragments arranged by size:
1281 1139 567 247 127 105 11
AseI AT'TA_AT
Cuts at: 0 1799 3477
Size: 1799 1678
AvaI C'yCGr_G
Cuts at: 0 622 2189 2833 3477
Size: 622 1567 644 644
Fragments arranged by size:
1567 644 644 622
BamHI G'GATC_C
Cuts at: 0 1223 2100 3477
Size: 1223 877 1377
Fragments arranged by size:
1377 1223 877
BanI G'GyrC_C
Cuts at: 0 598 3370 3477
Size: 598 2772 107
Fragments arranged by size:
2772 598 107
BbvCI CC'TCA_GC
Cuts at: 0 1981 3477
Size: 1981 1496
BciVI GTATCCnnnnn_n'
Cuts at: 0 731 1239 1869 3477
Size: 731 508 630 1608
Fragments arranged by size:
1608 731 630 508
BclI T'GATC_A
Cuts at: 0 138 1601 1634 3477
Size: 138 1463 33 1843
Fragments arranged by size:
1843 1463 138 33
BglII A'GATC_T
Cuts at: 0 688 1433 3477
Size: 688 745 2044
Fragments arranged by size:
2044 745 688
BlpI GC'TnA_GC
Cuts at: 0 218 1825 1994 3477
Size: 218 1607 169 1483
Fragments arranged by size:
1607 1483 218 169
Bme1580I G_kGCm'C
Cuts at: 0 3375 3477
Size: 3375 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1868 2959 3070 3406 3477
Size: 1868 1091 111 336 71
Fragments arranged by size:
1868 1091 336 111 71
Bpu10I CC'TnA_GC
Cuts at: 0 1981 3477
Size: 1981 1496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 3477
Size: 1001 2476
BsaI GGTCTCn'nnnn_
Cuts at: 0 717 3477
Size: 717 2760
BsaAI yAC'GTr
Cuts at: 0 777 3477
Size: 777 2700
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1841 1871 2338 2368 3477
Size: 1841 30 467 30 1109
Fragments arranged by size:
1841 1109 467 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1069 1639 3477
Size: 1069 570 1838
Fragments arranged by size:
1838 1069 570
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1235 3477
Size: 1042 193 2242
Fragments arranged by size:
2242 1042 193
BsiEI CG_ry'CG
Cuts at: 0 3203 3477
Size: 3203 274
BsmI GAATG_Cn'
Cuts at: 0 2745 3062 3477
Size: 2745 317 415
Fragments arranged by size:
2745 415 317
Bsp1286I G_dGCh'C
Cuts at: 0 3375 3477
Size: 3375 102
BspHI T'CATG_A
Cuts at: 0 2735 3477
Size: 2735 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1829 3477
Size: 1829 1648
BsrBI CCG'CTC
Cuts at: 0 1344 3477
Size: 1344 2133
BsrDI GCAATG_nn'
Cuts at: 0 74 1113 2798 3477
Size: 74 1039 1685 679
Fragments arranged by size:
1685 1039 679 74
BsrGI T'GTAC_A
Cuts at: 0 303 1494 3328 3477
Size: 303 1191 1834 149
Fragments arranged by size:
1834 1191 303 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 934 3477
Size: 934 2543
BstBI TT'CG_AA
Cuts at: 0 570 3477
Size: 570 2907
BstXI CCAn_nnnn'nTGG
Cuts at: 0 197 860 3477
Size: 197 663 2617
Fragments arranged by size:
2617 663 197
BstYI r'GATC_y
Cuts at: 0 688 1223 1433 2100 3477
Size: 688 535 210 667 1377
Fragments arranged by size:
1377 688 667 535 210
Bsu36I CC'TnA_GG
Cuts at: 0 46 3477
Size: 46 3431
BtgI C'CryG_G
Cuts at: 0 1730 2240 3314 3477
Size: 1730 510 1074 163
Fragments arranged by size:
1730 1074 510 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 721 3477
Size: 721 2756
BtsI GCAGTG_nn'
Cuts at: 0 3331 3477
Size: 3331 146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 12 47 3477
Size: 12 35 3430
Fragments arranged by size:
3430 35 12
DraI TTT'AAA
Cuts at: 0 941 1457 2726 3477
Size: 941 516 1269 751
Fragments arranged by size:
1269 941 751 516
EaeI y'GGCC_r
Cuts at: 0 33 3217 3297 3477
Size: 33 3184 80 180
Fragments arranged by size:
3184 180 80 33
EarI CTCTTCn'nnn_
Cuts at: 0 1234 1511 1916 2584 3477
Size: 1234 277 405 668 893
Fragments arranged by size:
1234 893 668 405 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2784 3477
Size: 2784 693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1868 1915 2072 2320 2959 3070 3271 3406
Size: 1868 47 157 248 639 111 201 135
Cuts at: 3406 3449 3477
Size: 43 28
Fragments arranged by size:
1868 639 248 201 157 135 111 47
43 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1656 2988 3477
Size: 1656 1332 489
Fragments arranged by size:
1656 1332 489
EcoRI G'AATT_C
Cuts at: 0 1528 3477
Size: 1528 1949
EcoRV GAT'ATC
Cuts at: 0 159 334 3477
Size: 159 175 3143
Fragments arranged by size:
3143 175 159
HaeII r_GCGC'y
Cuts at: 0 3105 3292 3477
Size: 3105 187 185
Fragments arranged by size:
3105 187 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 632 664 1843 1875 2814 2846 3477
Size: 632 32 1179 32 939 32 631
Fragments arranged by size:
1179 939 632 631 32 32 32
HincII GTy'rAC
Cuts at: 0 682 2286 2467 3477
Size: 682 1604 181 1010
Fragments arranged by size:
1604 1010 682 181
HindIII A'AGCT_T
Cuts at: 0 1081 1418 1508 1762 1927 2023 3477
Size: 1081 337 90 254 165 96 1454
Fragments arranged by size:
1454 1081 337 254 165 96 90
MfeI C'AATT_G
Cuts at: 0 3119 3477
Size: 3119 358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1223 1520 3477
Size: 638 585 297 1957
Fragments arranged by size:
1957 638 585 297
MscI TGG'CCA
Cuts at: 0 35 3219 3299 3477
Size: 35 3184 80 178
Fragments arranged by size:
3184 178 80 35
MslI CAynn'nnrTG
Cuts at: 0 667 2062 2095 3477
Size: 667 1395 33 1382
Fragments arranged by size:
1395 1382 667 33
MspA1I CmG'CkG
Cuts at: 0 1236 2526 2932 3058 3477
Size: 1236 1290 406 126 419
Fragments arranged by size:
1290 1236 419 406 126
NcoI C'CATG_G
Cuts at: 0 1730 2240 3477
Size: 1730 510 1237
Fragments arranged by size:
1730 1237 510
NspI r_CATG'y
Cuts at: 0 1015 1902 2400 2513 3439 3477
Size: 1015 887 498 113 926 38
Fragments arranged by size:
1015 926 887 498 113 38
PciI A'CATG_T
Cuts at: 0 2509 3435 3477
Size: 2509 926 42
Fragments arranged by size:
2509 926 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 1121 1539 3477
Size: 1121 418 1938
Fragments arranged by size:
1938 1121 418
PfoI T'CCnGG_A
Cuts at: 0 3383 3477
Size: 3383 94
PpuMI rG'GwC_Cy
Cuts at: 0 1656 2988 3477
Size: 1656 1332 489
Fragments arranged by size:
1656 1332 489
PspXI vC'TCGA_Gb
Cuts at: 0 2189 3477
Size: 2189 1288
PstI C_TGCA'G
Cuts at: 0 2476 3360 3477
Size: 2476 884 117
Fragments arranged by size:
2476 884 117
PvuII CAG'CTG
Cuts at: 0 1236 2526 2932 3058 3477
Size: 1236 1290 406 126 419
Fragments arranged by size:
1290 1236 419 406 126
SalI G'TCGA_C
Cuts at: 0 680 2465 3477
Size: 680 1785 1012
Fragments arranged by size:
1785 1012 680
ScaI AGT'ACT
Cuts at: 0 1840 3477
Size: 1840 1637
SexAI A'CCwGG_T
Cuts at: 0 594 707 3477
Size: 594 113 2770
Fragments arranged by size:
2770 594 113
SfcI C'TryA_G
Cuts at: 0 229 2472 3356 3477
Size: 229 2243 884 121
Fragments arranged by size:
2243 884 229 121
SmlI C'TyrA_G
Cuts at: 0 1016 2189 2833 3477
Size: 1016 1173 644 644
Fragments arranged by size:
1173 1016 644 644
SphI G_CATG'C
Cuts at: 0 1015 3477
Size: 1015 2462
SspI AAT'ATT
Cuts at: 0 1448 3477
Size: 1448 2029
StyI C'CwwG_G
Cuts at: 0 1730 2240 2600 3477
Size: 1730 510 360 877
Fragments arranged by size:
1730 877 510 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 3217 3358 3477
Size: 826 2391 141 119
Fragments arranged by size:
2391 826 141 119
TatI w'GTAC_w
Cuts at: 0 303 428 574 1494 1838 3328 3477
Size: 303 125 146 920 344 1490 149
Fragments arranged by size:
1490 920 344 303 149 146 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2338 2370 2400 2432 3212 3244 3477
Size: 2338 32 30 32 780 32 233
Fragments arranged by size:
2338 780 233 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 2281 3477
Size: 2281 1196
XbaI T'CTAG_A
Cuts at: 0 336 3477
Size: 336 3141
XhoI C'TCGA_G
Cuts at: 0 2189 2833 3477
Size: 2189 644 644
Fragments arranged by size:
2189 644 644
XmnI GAAnn'nnTTC
Cuts at: 0 248 1412 1520 3256 3477
Size: 248 1164 108 1736 221
Fragments arranged by size:
1736 1164 248 221 108
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AlwNI ApoI AseI
AvaI BamHI BanI BbvCI BciVI BclI BglII BlpI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NspI PciI PflMI PfoI
PpuMI PspXI PstI PvuII SalI ScaI SexAI SfcI
SmlI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BanII
BbeI BbsI BcgI BfrBI BglI BmgBI BmrI BmtI
BplI BsaBI BsaHI BsaWI BseYI BsiHKAI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstEII BstZ17I ClaI DraIII
DrdI EagI EcoICRI EcoNI FalI FseI FspI FspAI
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI XcmI XmaI
ZraI