Restriction map for
pF1KSDA0198
(Linear) (Six-base) MAPSORT of: KIAA0198.seq Check: 2136 from: 1 to: 1488
KIAA0198.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1488
Size: 544 944
AflIII A'CryG_T
Cuts at: 0 703 764 1488
Size: 703 61 724
Fragments arranged by size:
724 703 61
AleI CACnn'nnGTG
Cuts at: 0 575 925 1488
Size: 575 350 563
Fragments arranged by size:
575 563 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 1477 1488
Size: 943 534 11
Fragments arranged by size:
943 534 11
AlwNI CAG_nnn'CTG
Cuts at: 0 510 735 1344 1387 1488
Size: 510 225 609 43 101
Fragments arranged by size:
609 510 225 101 43
ApaLI G'TGCA_C
Cuts at: 0 634 943 1488
Size: 634 309 545
Fragments arranged by size:
634 545 309
ApoI r'AATT_y
Cuts at: 0 128 1488
Size: 128 1360
AvaI C'yCGr_G
Cuts at: 0 86 839 1488
Size: 86 753 649
Fragments arranged by size:
753 649 86
BanI G'GyrC_C
Cuts at: 0 428 553 623 895 1488
Size: 428 125 70 272 593
Fragments arranged by size:
593 428 272 125 70
BanII G_rGCy'C
Cuts at: 0 758 820 1198 1488
Size: 758 62 378 290
Fragments arranged by size:
758 378 290 62
BbeI G_GCGC'C
Cuts at: 0 432 1488
Size: 432 1056
BbsI GAAGACnn'nnnn_
Cuts at: 0 1425 1488
Size: 1425 63
BbvCI CC'TCA_GC
Cuts at: 0 163 212 466 1145 1331 1488
Size: 163 49 254 679 186 157
Fragments arranged by size:
679 254 186 163 157 49
BfrBI ATG'CAT
Cuts at: 0 444 1488
Size: 444 1044
BlpI GC'TnA_GC
Cuts at: 0 814 1007 1364 1488
Size: 814 193 357 124
Fragments arranged by size:
814 357 193 124
Bme1580I G_kGCm'C
Cuts at: 0 638 675 900 947 1488
Size: 638 37 225 47 541
Fragments arranged by size:
638 541 225 47 37
BmgBI CAC'GTC
Cuts at: 0 1439 1488
Size: 1439 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 1261 1488
Size: 1261 227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 992 1488
Size: 992 496
Bpu10I CC'TnA_GC
Cuts at: 0 163 212 466 1145 1331 1488
Size: 163 49 254 679 186 157
Fragments arranged by size:
679 254 186 163 157 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1355 1488
Size: 724 631 133
Fragments arranged by size:
724 631 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 189 1488
Size: 189 1299
BsaHI Gr'CG_yC
Cuts at: 0 429 1488
Size: 429 1059
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1295 1325 1488
Size: 1295 30 163
Fragments arranged by size:
1295 163 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1488
Size: 67 1421
BseYI C'CCAG_C
Cuts at: 0 417 674 916 1002 1130 1387 1488
Size: 417 257 242 86 128 257 101
Fragments arranged by size:
417 257 257 242 128 101 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1399 1488
Size: 202 1197 89
Fragments arranged by size:
1197 202 89
BsiEI CG_ry'CG
Cuts at: 0 591 1488
Size: 591 897
BsiHKAI G_wGCw'C
Cuts at: 0 521 638 947 1488
Size: 521 117 309 541
Fragments arranged by size:
541 521 309 117
BsmI GAATG_Cn'
Cuts at: 0 1480 1488
Size: 1480 8
Bsp1286I G_dGCh'C
Cuts at: 0 521 638 675 758 820 900 947 1198
Size: 521 117 37 83 62 80 47 251
Cuts at: 1198 1488
Size: 290
Fragments arranged by size:
521 290 251 117 83 80 62 47
37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1280 1488
Size: 1280 208
BsrFI r'CCGG_y
Cuts at: 0 425 590 1149 1488
Size: 425 165 559 339
Fragments arranged by size:
559 425 339 165
BstAPI GCAn_nnn'nTGC
Cuts at: 0 479 553 735 1186 1422 1488
Size: 479 74 182 451 236 66
Fragments arranged by size:
479 451 236 182 74 66
BstEII G'GTnAC_C
Cuts at: 0 460 1488
Size: 460 1028
BstXI CCAn_nnnn'nTGG
Cuts at: 0 682 1488
Size: 682 806
BstYI r'GATC_y
Cuts at: 0 1033 1488
Size: 1033 455
BtgI C'CryG_G
Cuts at: 0 758 881 959 1488
Size: 758 123 78 529
Fragments arranged by size:
758 529 123 78
BtsI GCAGTG_nn'
Cuts at: 0 381 582 1180 1488
Size: 381 201 598 308
Fragments arranged by size:
598 381 308 201
DraIII CAC_nnn'GTG
Cuts at: 0 630 1488
Size: 630 858
EaeI y'GGCC_r
Cuts at: 0 266 437 684 1488
Size: 266 171 247 804
Fragments arranged by size:
804 266 247 171
EarI CTCTTCn'nnn_
Cuts at: 0 712 803 1002 1488
Size: 712 91 199 486
Fragments arranged by size:
712 486 199 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 1105 1488
Size: 115 990 383
Fragments arranged by size:
990 383 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1488
Size: 544 944
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1210 1488
Size: 1210 278
EcoO109I rG'GnC_Cy
Cuts at: 0 375 507 1215 1488
Size: 375 132 708 273
Fragments arranged by size:
708 375 273 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1201 1488
Size: 1201 287
HaeII r_GCGC'y
Cuts at: 0 432 1488
Size: 432 1056
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 345 377 1295 1327 1488
Size: 345 32 918 32 161
Fragments arranged by size:
918 345 161 32 32
KasI G'GCGC_C
Cuts at: 0 428 1488
Size: 428 1060
MluI A'CGCG_T
Cuts at: 0 703 1488
Size: 703 785
MscI TGG'CCA
Cuts at: 0 268 439 1488
Size: 268 171 1049
Fragments arranged by size:
1049 268 171
MslI CAynn'nnrTG
Cuts at: 0 575 886 925 964 1488
Size: 575 311 39 39 524
Fragments arranged by size:
575 524 311 39 39
MspA1I CmG'CkG
Cuts at: 0 216 458 470 678 782 1123 1159 1488
Size: 216 242 12 208 104 341 36 329
Fragments arranged by size:
341 329 242 216 208 104 36 12
NaeI GCC'GGC
Cuts at: 0 1151 1488
Size: 1151 337
NarI GG'CG_CC
Cuts at: 0 429 1488
Size: 429 1059
NcoI C'CATG_G
Cuts at: 0 881 959 1488
Size: 881 78 529
Fragments arranged by size:
881 529 78
NgoMIV G'CCGG_C
Cuts at: 0 1149 1488
Size: 1149 339
NsiI A_TGCA'T
Cuts at: 0 446 1488
Size: 446 1042
NspI r_CATG'y
Cuts at: 0 448 768 876 891 1488
Size: 448 320 108 15 597
Fragments arranged by size:
597 448 320 108 15
PciI A'CATG_T
Cuts at: 0 764 1488
Size: 764 724
PflMI CCAn_nnn'nTGG
Cuts at: 0 1251 1387 1488
Size: 1251 136 101
Fragments arranged by size:
1251 136 101
PfoI T'CCnGG_A
Cuts at: 0 1098 1488
Size: 1098 390
PstI C_TGCA'G
Cuts at: 0 353 488 787 1488
Size: 353 135 299 701
Fragments arranged by size:
701 353 299 135
PvuII CAG'CTG
Cuts at: 0 216 470 782 1159 1488
Size: 216 254 312 377 329
Fragments arranged by size:
377 329 312 254 216
SapI GCTCTTCn'nnn_
Cuts at: 0 712 803 1488
Size: 712 91 685
Fragments arranged by size:
712 685 91
ScaI AGT'ACT
Cuts at: 0 667 1488
Size: 667 821
SfcI C'TryA_G
Cuts at: 0 349 484 783 1488
Size: 349 135 299 705
Fragments arranged by size:
705 349 299 135
SfoI GGC'GCC
Cuts at: 0 430 1488
Size: 430 1058
SmlI C'TyrA_G
Cuts at: 0 739 1370 1488
Size: 739 631 118
Fragments arranged by size:
739 631 118
SpeI A'CTAG_T
Cuts at: 0 72 1488
Size: 72 1416
SphI G_CATG'C
Cuts at: 0 448 1488
Size: 448 1040
StuI AGG'CCT
Cuts at: 0 84 168 773 862 1307 1488
Size: 84 84 605 89 445 181
Fragments arranged by size:
605 445 181 89 84 84
StyI C'CwwG_G
Cuts at: 0 557 857 881 959 1488
Size: 557 300 24 78 529
Fragments arranged by size:
557 529 300 78 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 286 1488
Size: 286 1202
TatI w'GTAC_w
Cuts at: 0 302 665 1488
Size: 302 363 823
Fragments arranged by size:
823 363 302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 643 675 1488
Size: 643 32 813
Fragments arranged by size:
813 643 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1305 1488
Size: 1071 234 183
Fragments arranged by size:
1071 234 183
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BfrBI BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MluI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PstI
PvuII SapI ScaI SfcI SfoI SmlI SpeI SphI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BglI BglII BmtI BpmI
BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MmeI NdeI NheI NotI NruI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI