Restriction map for
pF1KSDA0200
(Linear) (Six-base) MAPSORT of: KIAA0200.seq Check: 872 from: 1 to: 3048
KIAA0200.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 22 115 1123 3048
Size: 22 93 1008 1925
Fragments arranged by size:
1925 1008 93 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1924 2162 2805 2987 3010 3048
Size: 667 1257 238 643 182 23 38
Fragments arranged by size:
1257 667 643 238 182 38 23
AfeI AGC'GCT
Cuts at: 0 184 3048
Size: 184 2864
AgeI A'CCGG_T
Cuts at: 0 2469 3048
Size: 2469 579
AhdI GACnn_n'nnGTC
Cuts at: 0 843 3048
Size: 843 2205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 736 1223 3048
Size: 736 487 1825
Fragments arranged by size:
1825 736 487
AlwNI CAG_nnn'CTG
Cuts at: 0 107 1638 2689 2806 2907 3048
Size: 107 1531 1051 117 101 141
Fragments arranged by size:
1531 1051 141 117 107 101
ApaI G_GGCC'C
Cuts at: 0 1159 1304 2167 3048
Size: 1159 145 863 881
Fragments arranged by size:
1159 881 863 145
ApaLI G'TGCA_C
Cuts at: 0 2720 3048
Size: 2720 328
ApoI r'AATT_y
Cuts at: 0 901 2505 3048
Size: 901 1604 543
Fragments arranged by size:
1604 901 543
AseI AT'TA_AT
Cuts at: 0 887 3048
Size: 887 2161
AvaI C'yCGr_G
Cuts at: 0 136 292 1061 1269 1587 2126 3048
Size: 136 156 769 208 318 539 922
Fragments arranged by size:
922 769 539 318 208 156 136
AvrII C'CTAG_G
Cuts at: 0 1020 3048
Size: 1020 2028
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1999 2032 3048
Size: 1999 33 1016
Fragments arranged by size:
1999 1016 33
BanI G'GyrC_C
Cuts at: 0 92 249 783 1260 3048
Size: 92 157 534 477 1788
Fragments arranged by size:
1788 534 477 157 92
BanII G_rGCy'C
Cuts at: 0 761 1030 1128 1159 1304 2167 2683 2797
Size: 761 269 98 31 145 863 516 114
Cuts at: 2797 2833 2948 3048
Size: 36 115 100
Fragments arranged by size:
863 761 516 269 145 115 114 100
98 36 31
BbeI G_GCGC'C
Cuts at: 0 96 253 3048
Size: 96 157 2795
Fragments arranged by size:
2795 157 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 421 671 800 839 3048
Size: 421 250 129 39 2209
Fragments arranged by size:
2209 421 250 129 39
BbvCI CC'TCA_GC
Cuts at: 0 2771 3048
Size: 2771 277
BclI T'GATC_A
Cuts at: 0 680 3048
Size: 680 2368
BglI GCCn_nnn'nGGC
Cuts at: 0 241 262 1025 1258 1618 2270 2661 3048
Size: 241 21 763 233 360 652 391 387
Fragments arranged by size:
763 652 391 387 360 241 233 21
BlpI GC'TnA_GC
Cuts at: 0 2682 3048
Size: 2682 366
Bme1580I G_kGCm'C
Cuts at: 0 788 1159 1304 2087 2167 2657 2724 3048
Size: 788 371 145 783 80 490 67 324
Fragments arranged by size:
788 783 490 371 324 145 80 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 605 695 1070 1142 2007 2707 3048
Size: 605 90 375 72 865 700 341
Fragments arranged by size:
865 700 605 375 341 90 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1197 1439 1680 3048
Size: 1197 242 241 1368
Fragments arranged by size:
1368 1197 242 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 172 452 524 564 893 1180 1219
Size: 166 6 280 72 40 329 287 39
Cuts at: 1219 1429 2100 2574 3048
Size: 210 671 474 474
Fragments arranged by size:
671 474 474 329 287 280 210 166
72 40 39 6
Bpu10I CC'TnA_GC
Cuts at: 0 2771 2791 2807 3048
Size: 2771 20 16 241
Fragments arranged by size:
2771 241 20 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 3047 3048
Size: 3047 1
BsaHI Gr'CG_yC
Cuts at: 0 93 250 278 512 3048
Size: 93 157 28 234 2536
Fragments arranged by size:
2536 234 157 93 28
BsaWI w'CCGG_w
Cuts at: 0 2469 3048
Size: 2469 579
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1807 1837 3048
Size: 1807 30 1211
Fragments arranged by size:
1807 1211 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 781 841 3022 3048
Size: 781 60 2181 26
Fragments arranged by size:
2181 781 60 26
BseYI C'CCAG_C
Cuts at: 0 1015 1450 1631 1784 2143 2303 2423 2748
Size: 1015 435 181 153 359 160 120 325
Cuts at: 2748 2796 2811 3048
Size: 48 15 237
Fragments arranged by size:
1015 435 359 325 237 181 160 153
120 48 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 2848 2853 3048
Size: 160 2688 5 195
Fragments arranged by size:
2688 195 160 5
BsiEI CG_ry'CG
Cuts at: 0 303 2853 3048
Size: 303 2550 195
Fragments arranged by size:
2550 303 195
BsiHKAI G_wGCw'C
Cuts at: 0 299 761 2683 2724 3048
Size: 299 462 1922 41 324
Fragments arranged by size:
1922 462 324 299 41
BsmI GAATG_Cn'
Cuts at: 0 2534 3048
Size: 2534 514
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2382 3048
Size: 2382 666
Bsp1286I G_dGCh'C
Cuts at: 0 299 761 788 1030 1128 1159 1304 2087
Size: 299 462 27 242 98 31 145 783
Cuts at: 2087 2167 2657 2683 2724 2797 2833 2948 3048
Size: 80 490 26 41 73 36 115 100
Fragments arranged by size:
783 490 462 299 242 145 115 100
98 80 73 41 36 31 27 26
BspHI T'CATG_A
Cuts at: 0 321 1394 3048
Size: 321 1073 1654
Fragments arranged by size:
1654 1073 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 22 115 677 1123 1472 2040 3048
Size: 22 93 562 446 349 568 1008
Fragments arranged by size:
1008 568 562 446 349 93 22
BsrFI r'CCGG_y
Cuts at: 0 71 89 1092 2469 3048
Size: 71 18 1003 1377 579
Fragments arranged by size:
1377 1003 579 71 18
BssHII G'CGCG_C
Cuts at: 0 207 3048
Size: 207 2841
BssSI C'ACGA_G
Cuts at: 0 1654 3048
Size: 1654 1394
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1875 2781 3048
Size: 1875 906 267
Fragments arranged by size:
1875 906 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1051 3048
Size: 1051 1997
Bsu36I CC'TnA_GG
Cuts at: 0 2138 3048
Size: 2138 910
BtgI C'CryG_G
Cuts at: 0 20 40 239 1334 1802 2344 2492 2666
Size: 20 20 199 1095 468 542 148 174
Cuts at: 2666 2693 2894 3048
Size: 27 201 154
Fragments arranged by size:
1095 542 468 201 199 174 154 148
27 20 20
BtsI GCAGTG_nn'
Cuts at: 0 2579 2657 2865 2946 3048
Size: 2579 78 208 81 102
Fragments arranged by size:
2579 208 102 81 78
EaeI y'GGCC_r
Cuts at: 0 300 308 1596 1725 1805 2544 3048
Size: 300 8 1288 129 80 739 504
Fragments arranged by size:
1288 739 504 300 129 80 8
EagI C'GGCC_G
Cuts at: 0 300 3048
Size: 300 2748
EarI CTCTTCn'nnn_
Cuts at: 0 456 3048
Size: 456 2592
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 1081 3048
Size: 40 1041 1967
Fragments arranged by size:
1967 1041 40
EcoICRI GAG'CTC
Cuts at: 0 759 2681 3048
Size: 759 1922 367
Fragments arranged by size:
1922 759 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 172 452 524 564 667 893 1180
Size: 166 6 280 72 40 103 226 287
Cuts at: 1180 1219 1429 1924 2100 2162 2574 2805 2987
Size: 39 210 495 176 62 412 231 182
Cuts at: 2987 3010 3048
Size: 23 38
Fragments arranged by size:
495 412 287 280 231 226 210 182
176 166 103 72 62 40 39 38
23 6
EcoO109I rG'GnC_Cy
Cuts at: 0 393 429 804 837 982 1155 1166 1300
Size: 393 36 375 33 145 173 11 134
Cuts at: 1300 1301 1435 1781 2069 2164 2441 2699 2803
Size: 1 134 346 288 95 277 258 104
Cuts at: 2803 2816 3048
Size: 13 232
Fragments arranged by size:
393 375 346 288 277 258 232 173
145 134 134 104 95 36 33 13
11 1
EcoRI G'AATT_C
Cuts at: 0 901 2505 3048
Size: 901 1604 543
Fragments arranged by size:
1604 901 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1389 3048
Size: 1389 1659
HaeII r_GCGC'y
Cuts at: 0 66 96 144 159 186 253 353 1992
Size: 66 30 48 15 27 67 100 1639
Cuts at: 1992 2741 3048
Size: 749 307
Fragments arranged by size:
1639 749 307 100 67 66 48 30
27 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 431 463 829 861 1608 1640
Size: 115 32 284 32 366 32 747 32
Cuts at: 1640 2835 2867 3048
Size: 1195 32 181
Fragments arranged by size:
1195 747 366 284 181 115 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 92 249 3048
Size: 92 157 2799
Fragments arranged by size:
2799 157 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2034 2135 3048
Size: 2034 101 913
Fragments arranged by size:
2034 913 101
MscI TGG'CCA
Cuts at: 0 1598 1727 1807 2546 3048
Size: 1598 129 80 739 502
Fragments arranged by size:
1598 739 502 129 80
MslI CAynn'nnrTG
Cuts at: 0 320 593 3048
Size: 320 273 2455
Fragments arranged by size:
2455 320 273
MspA1I CmG'CkG
Cuts at: 0 42 283 1638 1855 2120 2383 2689 2896
Size: 42 241 1355 217 265 263 306 207
Cuts at: 2896 3048
Size: 152
Fragments arranged by size:
1355 306 265 263 241 217 207 152
42
NaeI GCC'GGC
Cuts at: 0 73 91 1094 3048
Size: 73 18 1003 1954
Fragments arranged by size:
1954 1003 73 18
NarI GG'CG_CC
Cuts at: 0 93 250 3048
Size: 93 157 2798
Fragments arranged by size:
2798 157 93
NcoI C'CATG_G
Cuts at: 0 20 1334 2492 2666 3048
Size: 20 1314 1158 174 382
Fragments arranged by size:
1314 1158 382 174 20
NgoMIV G'CCGG_C
Cuts at: 0 71 89 1092 3048
Size: 71 18 1003 1956
Fragments arranged by size:
1956 1003 71 18
NspI r_CATG'y
Cuts at: 0 2176 2253 2335 2604 3048
Size: 2176 77 82 269 444
Fragments arranged by size:
2176 444 269 82 77
PasI CC'CwG_GG
Cuts at: 0 2554 3048
Size: 2554 494
PpuMI rG'GwC_Cy
Cuts at: 0 393 804 837 1781 2069 2441 2699 3048
Size: 393 411 33 944 288 372 258 349
Fragments arranged by size:
944 411 393 372 349 288 258 33
PspOMI G'GGCC_C
Cuts at: 0 1155 1300 2163 3048
Size: 1155 145 863 885
Fragments arranged by size:
1155 885 863 145
PspXI vC'TCGA_Gb
Cuts at: 0 2126 3048
Size: 2126 922
PstI C_TGCA'G
Cuts at: 0 521 966 2586 3048
Size: 521 445 1620 462
Fragments arranged by size:
1620 521 462 445
PvuII CAG'CTG
Cuts at: 0 1638 2120 2383 2689 3048
Size: 1638 482 263 306 359
Fragments arranged by size:
1638 482 359 306 263
RsrII CG'GwC_CG
Cuts at: 0 2891 3048
Size: 2891 157
SacI G_AGCT'C
Cuts at: 0 761 2683 3048
Size: 761 1922 365
Fragments arranged by size:
1922 761 365
SacII CC_GC'GG
Cuts at: 0 43 2897 3048
Size: 43 2854 151
Fragments arranged by size:
2854 151 43
SanDI GG'GwC_CC
Cuts at: 0 1781 2441 3048
Size: 1781 660 607
Fragments arranged by size:
1781 660 607
SfcI C'TryA_G
Cuts at: 0 517 962 2582 2919 3048
Size: 517 445 1620 337 129
Fragments arranged by size:
1620 517 445 337 129
SfoI GGC'GCC
Cuts at: 0 94 251 3048
Size: 94 157 2797
Fragments arranged by size:
2797 157 94
SgrAI Cr'CCGG_yG
Cuts at: 0 71 3048
Size: 71 2977
SmaI CCC'GGG
Cuts at: 0 1063 1271 1589 3048
Size: 1063 208 318 1459
Fragments arranged by size:
1459 1063 318 208
SmlI C'TyrA_G
Cuts at: 0 2126 3048
Size: 2126 922
SspI AAT'ATT
Cuts at: 0 891 3048
Size: 891 2157
StuI AGG'CCT
Cuts at: 0 2878 3048
Size: 2878 170
StyI C'CwwG_G
Cuts at: 0 20 197 872 1020 1334 2099 2476 2492
Size: 20 177 675 148 314 765 377 16
Cuts at: 2492 2666 3048
Size: 174 382
Fragments arranged by size:
765 675 382 377 314 177 174 148
20 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 2887 3048
Size: 472 2415 161
Fragments arranged by size:
2415 472 161
TatI w'GTAC_w
Cuts at: 0 1003 3048
Size: 1003 2045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1807 1839 2088 2120 3048
Size: 1807 32 249 32 928
Fragments arranged by size:
1807 928 249 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 2664 3048
Size: 18 2646 384
Fragments arranged by size:
2646 384 18
XhoI C'TCGA_G
Cuts at: 0 2126 3048
Size: 2126 922
XmaI C'CCGG_G
Cuts at: 0 1061 1269 1587 3048
Size: 1061 208 318 1461
Fragments arranged by size:
1461 1061 318 208
Enzymes that do cut:
AarI AcuI AfeI AgeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AseI AvaI AvrII BaeI BanI BanII
BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrFI BssHII BssSI BstAPI BstXI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PpuMI
PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI
SfcI SfoI SgrAI SmaI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AleI AloI
AscI AsiSI BamHI BcgI BciVI BfrBI BglII BmgBI
BmtI BpuEI BsaAI BsaBI BsiWI BspEI BsrBI BsrDI
BsrGI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoNI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI SalI SapI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XmnI ZraI